NOTE: For this lab to run smoothly, it is recommended to not exceed 12 samples per laboratory class.
First gather the necessary materials, including popsicle sticks, funnels, 15 mL conical tubes, micropipettes, ice buckets with ice, small cups and microcentrifuge tubes.
Next set the water bath used in part one of the laboratory exercise, to 56 °C.
Set a second water bath to be used in part two of the laboratory exercise to 37 °C.
To make TE buffer, first measure out 400 mL of RO water.
Then add five mL of 1M Tris-HCL pH 8, and then 1 mL of 0.5 M EDTA solution.
Next, add sufficient RO water to bring the solution up to 500 mL.
To make TE buffer with SDS or TES buffer, again measure out 400 mL of RO water.
Add five milliliters of 1M Tris-HCL pH 8, and then add 1 mL of 0.5 molar EDTA solution.
Now add 5 mL of 10% SDS solution.
Bring the volume up to 500 mL, using RO water.
Next, prepare the 1% saline solution that the students will use to collect cheek cells. In an empty, clean soda bottle, dissolve 5 g of NaCl in 500 mL of RO water.
Pour 10 mL aliquots into small drinking cups for the students to use, one per student group.
After this, prepare a sufficient quantity of 10 mL aliquots of 100% ethanol for the students to use. NOTE: Store these ethanol aliquots in the freezer or refrigerator to keep them preferably ice-cold.