Jove Lab Chem

Lab 58: Column Chromatography — Materials List

Preparation for 10 Students

• 50-mL glass beaker
  25
• 100-mL glass beaker
  5
• 5- or 10-mL glass graduated cylinder
  5
• 50-mL glass graduated cylinder
  10
• Small powder funnel
  10
• Glass stirring rod
  5
• Labeling tape
  5 rolls
• Labeling pen
  5
• Ruler
  5
• Pasteur pipette bulbs
  10
• Laboratory stand
  5
• Burette clamp
  5
• 50-mL glass burette
  5
• Weighing boats
  20
• Cotton or glass wool plugs
  10
• Long-stem Pasteur pipettes
  50
• Sand (40-100 mesh)
  15 g
• Silica gel powder for column chromatography (35-70 mesh, 100 grade)
  50 g
• Ethanol (95%)
  400 mL
• Green food coloring containing tartrazine and erioglaucine
  5 mL
• 5-mL glass volumetric flask with cap
  1
• Long blunt-ended wooden/metal rod
  1
• 250-mL plastic wash bottle
  1
• Paraffin film
  1 box
• Spatulas
  5
• Lab wipes
  3 boxes
• Acetone
  250 mL
• Analytical balance (at least 1)
  Dependent on lab size
• Deionized water
  Dependent on lab size