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Rat Intestinal Crypts Isolation and Intestinal Organoids Generation

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Establishment and Passaging of Small Intestine Organoids

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Establishment and Passaging of Small Intestine Organoids

Establishment and Passaging of Small Intestine Organoids

Rat Intestinal Crypts Isolation and Intestinal Organoids Generation

DOI:

10.3791/200273-v

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05:32 min

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June 23, 2023

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Eleanor Zagoren*¹, Anderson K. Santos*^1,2, Nadia A. Ameen⁴, Kaelyn Sumigray^5,6

¹Department of Genetics,Yale School of Medicine, ²Department of Pediatrics,Yale School of Medicine, ³Department of Pediatrics/Gastroenterology and Hepatology,Yale School of Medicine, ⁴Department of Cellular and Molecular Physiology,Yale School of Medicine, ⁵Yale Stem Cell Center,Yale School of Medicine, ⁶Yale Cancer Center,Yale School of Medicine

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Intestinal Organoids Small Intestine Rat Dissection Intestinal Fragment Mesentery PBS EDTA Epithelium Villi Crypts Cell Culture

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