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Mouse Hindbrain Ex Vivo Culture to Study Facial Branchiomotor Neuron Migration
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Capitoli
- 00:05Titolo
- 01:26Coating of Culture Inserts
- 02:08Dissection of Hindbrains from E11.5 Mouse Embryos
- 05:40Hindbrain Explant Culture
- 07:26Wholemount Immunofluorescent Staining of Hindbrain Explants
- 09:00Results: Neuronal Migration in Mouse Hindbrain in Ex Vivo Culture
- 10:28Conclusion
Embryonic neurons are born in the ventricular zone of the neural tube, but migrate to reach appropriate targets. Facial branchiomotor (FBM) neurons are a useful model to study neuronal migration. This protocol describes the wholemount ex vivo culture of mouse embryo hindbrains to investigate mechanisms that regulate FBM migration.
