Generazione di Genomic eliminazioni in linee cellulari di mammiferi via CRISPR / Cas9
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January 03, 2015
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CRISPR/Cas9 is a robust system to produce disruption of genes and genetic elements. Here we describe a protocol for the efficient creation of genomic deletions in mammalian cell lines using CRISPR/Cas9.
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Bauer, D. E., Canver, M. C., Orkin, S. H. Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9. J. Vis. Exp. (95), e52118, doi:10.3791/52118 (2015).
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