Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / 利用相扑融合策略生成国产、未加标签的杭丁顿蛋白 Exon1…
JoVE Journal
Biochemistry

This Contenuto is Open Access.

JoVE Journal Biochemistry
利用相扑融合策略生成国产、未加标签的杭丁顿蛋白 Exon1 单体和纤维
Preparing Electron Microscopy (EM) Grids for Cellular Cryotomography (Video) | JoVE
Journal (Biochemistry)
Preparing Electron Microscopy (EM) Grids for Cellular Cryotomography (Video) | JoVE
Ion Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization
Journal (Biochemistry)
Ion Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization
 
Click here for the English version

利用相扑融合策略生成国产、未加标签的杭丁顿蛋白 Exon1 单体和纤维

Article DOI: 10.3791/57506-v • 11:22 min • June 27th, 2018
June 27th, 2018 •
1, 1, 1, 1
1Laboratory of Molecular and Chemical Biology of Neurodegeneration, Ecole Polytechnique Fédérale de Lausanne (EPFL)

Capitoli

Riepilogo
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

在这里, 我们提出了一个健壮和优化的协议, 以生产毫克数量的本地, 无标签单体和纤维的 exon1 的杭丁顿蛋白蛋白 (Httex1) 的基础上的瞬态融合的小泛素相关修饰剂 (相扑)。

Tags

生物化学 问题 136 HD 亨廷顿氏病 杭丁顿蛋白 变性 polyglutamine polyQ 聚合 纤维 相扑融合技术 蛋白质的表达和纯化
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter