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Hyaluronic-Acid Based Hydrogels for 3-Dimensional Culture of Patient-Derived Glioblastoma Cells

Article DOI: 10.3791/58176-v • 08:35 min • August 24th, 2018
August 24th, 2018 •
1, 1, 1, 2
1Department of Bioengineering, University of California, Los Angeles, 2Department of Bioengineering, Jonsson Comprehensive Cancer Center, Broad Stem Cell Research Center, Brain Research Institute, University of California, Los Angeles

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Here, we present a protocol for three-dimensional culture of patient-derived glioblastoma cells within orthogonally tunable biomaterials designed to mimic the brain matrix. This approach provides an in vitro, experimental platform that maintains many characteristics of in vivo glioblastoma cells typically lost in culture.

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Hyaluronic-acid Hydrogels 3-dimensional Culture Patient-derived Glioblastoma Cells Extracellular Matrix Treatment Resistance Cell Culture Platforms Native Brain Silicone Rubber Molds Non-tissue Culture Treated Plate Pipette Tip Centrifugation Cell Dissociation Enzyme Complete Medium Cell Straining 70 Micrometer Filter Polyethylene Glycol Maleimide (PEG-Mal-RGD)
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