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Differentierade musadipocyter i primärkultur
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Differentierade musadipocyter i primärkultur: En modell av insulinresistens

Article DOI: 10.3791/63979-v • 09:48 min • February 17th, 2023
February 17th, 2023 •
1, 1, 1, 1, 1
1Instituto de Neurobiología, Universidad Nacional Autónoma de México (UNAM)

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Detta protokoll beskriver isoleringen av muspreadipocyter från subkutant fett, deras differentiering till mogna adipocyter och induktion av insulinresistens. Insulinverkan utvärderas genom fosforylering/aktivering av medlemmar av insulinsignalvägen genom western blot. Denna metod möjliggör direkt bestämning av insulinresistens/känslighet i primära adipocyter.

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Biologi utgåva 192 preadipocytisolering subkutant fett insulinverkan TNF-α
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