/

/

A Protocol to Isolate, Culture, and Identify Human Limbal Niche Cells

/

Limbal Niş Hücrelerin İzolasyonu ve Tanımlanması

JoVE Journal
Medicina
Author Produced

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Medicina

Isolation and Identification of Limbal Niche Cells

Please note that all translations are automatically generated. Click here for the English version.

Limbal Niş Hücrelerin İzolasyonu ve Tanımlanması

A Protocol to Isolate, Culture, and Identify Human Limbal Niche Cells

DOI:

10.3791/65618-v

•

01:28 min

•

October 27, 2023

•

Guanyu Su, Wei Wang, Lingjuan Xu, Rong Liu, Yongyao Tan, Bihui Jin, Chunxiu You, Xiao Zhou, Yihong Xiong, Huatao Xie, Guigang Li

¹Department of Ophthalmology, Tongji Hospital, Tongji Medical College,Huazhong University of Science and Technology, ²Institute of Medicine Nursing,Hubei University of Medicine, ³Department of Ophthalmology, Union Hospital, Tongji Medical College,Huazhong University of Science and Technology

Tags

Limbal Niche Cells Limbal Stem Cell Deficiency Single Cell Sequencing Mesenchymal Stem Cells Collagenase A Trypsin-EDTA Modified Embryonic Stem Cell Medium Matrigel Immunofluorescence Real-time Quantitative PCR Flow Cytometry Stem Cell Markers VIM CD90 CD105 PDGFR-beta Pan-CK CD73 SCF

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Limbal Niş Hücrelerin İzolasyonu ve Tanımlanması

Limbal Niş Hücrelerin (LNC'ler) Limbus Dokusundan İzolasyonu

Limbal Niş Hücrelerin (LNC'ler) Tanımlanması

Read Article