Hydrogel Preparation and Channel Embedding of a Vessels-on-a-Plate (VOP) Platform for the Culture of Human Umbilical Vein Endothelial Cells (HUVEC)

To begin dissolve, 0.01 grams of LAP into a 50 milliliter conical tube containing 20 milliliters of PBS. Add three grams of gelatin methacrylate and 0.2 grams of fibrinogen to the LAP solution. Place the mixture in a 37 degree Celsius water bath.

Add 300 microliters of the pre warmed gel fib into each hydrogel chamber of the VOP platform to embed the sacrificial pattern. Use UV light to rapidly cross-link the gelatin methacrylate. After repeating the UV cross-linking for all other wells, pipette one milliliter of DPBS into each side of the vascular channel of each well.

Place the plate at four degrees Celsius for 15 minutes to liquefy the Pluronic F127. Before introducing cells into the channels profuse DMEMF12 containing 1%matcha gel through the channels for 30 minutes.