An Ex Vivo Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time

Starlyn L. M. Okada; Nicole S. Stivers; Peter K. Stys; David P. Stirling

doi:10.3791/52173

ジャーナル

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神経科学

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アンエクスビボレーザー誘起脊髄損傷モデルがリアルタイムで軸索変性のメカニズムを評価するために

An <em>Ex Vivo</em> Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time

JoVE Journal
神経科学

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JoVE Journal 神経科学

An Ex Vivo Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time

アンエクスビボレーザー誘起脊髄損傷モデルがリアルタイムで軸索変性のメカニズムを評価するために

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10,816 Views

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11:18 min

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November 25, 2014

DOI:

10.3791/52173-v

DOI:

10.3791/52173-v

•

11:18 min

November 25, 2014

•

2 Views

Starlyn L. M. Okada¹, Nicole S. Stivers¹, Peter K. Stys², David P. Stirling¹

¹KY Spinal Cord Injury Research Center, Department of Neurological Surgery,University of Louisville, ²Hotchkiss Brain Institute, Department of Clinical Neurosciences,University of Calgary

概要

We present a protocol utilizing two-photon excitation time-lapse microscopy to simultaneously visualize the dynamics of axon and myelin injuries in real time. This proposed protocol permits studies of both intrinsic and extrinsic factors which can influence central myelinated axon fate after injury and contribute to permanent clinical disability.