Recording Temperature-induced Neuronal Activity through Monitoring Calcium Changes in the Olfactory Bulb of <em>Xenopus laevis</em>

Alexander Brinkmann; Camille Okom; Eugen Kludt; Detlev Schild

doi:10.3791/54108

ジャーナル

/

神経科学

/

の嗅球にカルシウム変化をモニタリングを通じて温度によって誘発される神経活動を記録<em>アフリカツメガエル</em

Recording Temperature-induced Neuronal Activity through Monitoring Calcium Changes in the Olfactory Bulb of <em>Xenopus laevis</em>

JoVE Journal
神経科学

This content is Free Access.

JoVE Journal 神経科学

Recording Temperature-induced Neuronal Activity through Monitoring Calcium Changes in the Olfactory Bulb of Xenopus laevis

の嗅球にカルシウム変化をモニタリングを通じて温度によって誘発される神経活動を記録<em>アフリカツメガエル</em

Please note that all translations are automatically generated. Click here for the English version.

7,266 Views

•

11:08 min

•

June 03, 2016

DOI:

10.3791/54108-v

DOI:

10.3791/54108-v

•

11:08 min

June 03, 2016

•

7246 Views

Alexander Brinkmann*^1,2, Camille Okom*^1,2, Eugen Kludt^1,2,4, Detlev Schild^1,2,3

¹Institute of Neurophysiology and Cellular Biophysics,Georg-August-Universität Göttingen ²Center for Nanoscale Microscopy and Molecular Physiology of the Brain,Georg-August-Universität Göttingen ³DFG Excellence Cluster 171,Georg-August-Universität Göttingen ⁴German Hearing Center Hannover

概要

Here we describe a protocol for measuring and analyzing temperature responses in the olfactory bulb of Xenopus laevis. Olfactory receptor neurons and mitral cells are differentially stained, after which calcium changes are recorded, reflecting a sensitivity of some neural networks in the bulb to temperature drops induced at the nose.