-1::1
Simple Hit Counter
Skip to content

Products

Solutions

×
×
Sign In

JA

EN - EnglishCN - 简体中文DE - DeutschES - EspañolKR - 한국어IT - ItalianoFR - FrançaisPT - Português do BrasilPL - PolskiHE - עִבְרִיתRU - РусскийJA - 日本語TR - TürkçeAR - العربية
Sign In Start Free Trial

RESEARCH

JoVE Journal

Peer reviewed scientific video journal

Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
View All
JoVE Encyclopedia of Experiments

Video encyclopedia of advanced research methods

Biological Techniques
Biology
Cancer Research
Immunology
Neuroscience
Microbiology
JoVE Visualize

Visualizing science through experiment videos

EDUCATION

JoVE Core

Video textbooks for undergraduate courses

Analytical Chemistry
Anatomy and Physiology
Biology
Calculus
Cell Biology
Chemistry
Civil Engineering
Electrical Engineering
View All
JoVE Science Education

Visual demonstrations of key scientific experiments

Advanced Biology
Basic Biology
Chemistry
View All
JoVE Lab Manual

Videos of experiments for undergraduate lab courses

Biology
Chemistry

BUSINESS

JoVE Business

Video textbooks for business education

Accounting
Finance
Macroeconomics
Marketing
Microeconomics

OTHERS

JoVE Quiz

Interactive video based quizzes for formative assessments

Authors

Teaching Faculty

Librarians

K12 Schools

Biopharma

Products

RESEARCH

JoVE Journal

Peer reviewed scientific video journal

JoVE Encyclopedia of Experiments

Video encyclopedia of advanced research methods

JoVE Visualize

Visualizing science through experiment videos

EDUCATION

JoVE Core

Video textbooks for undergraduates

JoVE Science Education

Visual demonstrations of key scientific experiments

JoVE Lab Manual

Videos of experiments for undergraduate lab courses

BUSINESS

JoVE Business

Video textbooks for business education

OTHERS

JoVE Quiz

Interactive video based quizzes for formative assessments

Solutions

Authors
Teaching Faculty
Librarians
K12 Schools
Biopharma

Language

ja

EN

English

CN

简体中文

DE

Deutsch

ES

Español

KR

한국어

IT

Italiano

FR

Français

PT

Português do Brasil

PL

Polski

HE

עִבְרִית

RU

Русский

JA

日本語

TR

Türkçe

AR

العربية

    Menu

    JoVE Journal

    Behavior

    Biochemistry

    Bioengineering

    Biology

    Cancer Research

    Chemistry

    Developmental Biology

    Engineering

    Environment

    Genetics

    Immunology and Infection

    Medicine

    Neuroscience

    Menu

    JoVE Encyclopedia of Experiments

    Biological Techniques

    Biology

    Cancer Research

    Immunology

    Neuroscience

    Microbiology

    Menu

    JoVE Core

    Analytical Chemistry

    Anatomy and Physiology

    Biology

    Calculus

    Cell Biology

    Chemistry

    Civil Engineering

    Electrical Engineering

    Introduction to Psychology

    Mechanical Engineering

    Medical-Surgical Nursing

    View All

    Menu

    JoVE Science Education

    Advanced Biology

    Basic Biology

    Chemistry

    Clinical Skills

    Engineering

    Environmental Sciences

    Physics

    Psychology

    View All

    Menu

    JoVE Lab Manual

    Biology

    Chemistry

    Menu

    JoVE Business

    Accounting

    Finance

    Macroeconomics

    Marketing

    Microeconomics

Start Free Trial
Loading...
Home
JoVE Journal
Bioengineering
大麻のクローン伝播のためのエアロポニックシステムの採用
大麻のクローン伝播のためのエアロポニックシステムの採用
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Employing Aeroponic Systems for the Clonal Propagation of Cannabis

大麻のクローン伝播のためのエアロポニックシステムの採用

Full Text
4,696 Views
03:41 min
December 1, 2021

DOI: 10.3791/63117-v

Trevor Regas1, Joon-Hee Han2, Christopher S. Pauli3, Sang-Hyuck Park1,3

1Biology Department,Colorado State University-Pueblo, 2Chuncheon Bioindustry Foundation, 3Institute of Cannabis Research,Colorado State University-Pueblo

AI Banner

Please note that some of the translations on this page are AI generated. Click here for the English version.

Overview

This protocol provides instructional information for the clonal propagation of Cannabis sativa L. using aeroponic systems. It aims to induce early root formation in cannabis cuttings while minimizing susceptibility to pathogens.

Key Study Components

Area of Science

  • Plant propagation
  • Aeroponic systems
  • Cannabis cultivation

Background

  • Propagation of Cannabis sativa L. is essential for research and commercial production.
  • Inducing early root formation can enhance survival rates of cuttings.
  • Minimizing pathogen susceptibility is crucial for successful cultivation.
  • This method offers a systematic approach to achieve desirable traits in cannabis.

Purpose of Study

  • To provide a reliable protocol for cannabis propagation.
  • To improve the efficiency of cannabis cultivation techniques.
  • To facilitate the regeneration of desired cannabis genetics.

Methods Used

  • Use of a sterilized scalpel or scissors for cutting shoots.
  • Cutting shoots to approximately 10 centimeters in length.
  • Removing foliage except for the top three nodes.
  • Dipping cuttings into a rooting solution containing indole-3-butyric acid.

Main Results

  • Early root formation was successfully induced in cannabis cuttings.
  • Reduced susceptibility to pathogens was observed.
  • The protocol can be applied in both research and commercial settings.
  • Desirable morphological and chemical properties were reproduced.

Conclusions

  • This method enhances the propagation of Cannabis sativa L.
  • It provides a systematic approach for researchers and cultivators.
  • Future applications may improve cannabis genetics and production.

Frequently Asked Questions

What is the main advantage of this propagation method?
The main advantage is inducing early root formation while limiting susceptibility to pathogens.
What supplies are needed for this protocol?
You will need a sterilized scalpel or scissors, rooting solution, and an aeroponic system.
How long should the cuttings be?
Cuttings should be approximately 10 centimeters in length.
What should be done with the foliage on the cuttings?
Remove all foliage except for the top three nodes.
What is indole-3-butyric acid used for?
It is used in the rooting solution to promote root formation in cuttings.
Can this method be used for commercial production?
Yes, this method is applicable in both research and commercial settings.

このプロトコルは、エアロポニックシステムを実装することにより 、大麻サティバ L.のクローン伝播のための教育情報を提供するように設計されています。ここで説明する方法は 、大麻属の望ましい形態学的および化学的特性を正常に再現するために必要なすべての供給およびプロトコルを含む。

このプロトコルは、研究や商業生産の設定で所望の大麻遺伝学の再生を進める方法に関する情報に基づいた意思決定を行う際に役立つことができます。この技術の主な利点は、潜在的な病原体への感受性を制限しながら、大麻挿し木の早期根形成を誘導することです。この技術の影響は、研究や生産の設定で適用することができる挿し木の早期根形成を誘導することによって大麻の伝播で観察される課題の改善に向かって拡張します。

滅菌メスまたはハサミで、頭頂部メリステムの近くの長さ約10センチメートルの数個の節を斜め45度で切ります。上の 3 つのノードに存在するフォリーを除くすべての葉を削除します。新たに切り取った切断を茎の基部から約2〜5センチメートル上に浸し、インドール-3酪酸を含む根付き溶液に約5秒間浸します。

View the full transcript and gain access to thousands of scientific videos

View the full transcript and gain access to thousands of scientific videos

Sign In Start Free Trial

Explore More Videos

バイオエンジニアリング 問題178

Related Videos

HSPCクローンの培養と採取:シングルソーティングされたHSPCクローンの入手方法

05:21

HSPCクローンの培養と採取:シングルソーティングされたHSPCクローンの入手方法

Related Videos

1.6K Views

側根誘導系でシロイヌナズナとトウモロコシ

09:23

側根誘導系でシロイヌナズナとトウモロコシ

Related Videos

14.6K Views

水耕:栄養アベイラビリティと毒性元素への曝露に栄養の割り当てと植物の応答を研究するための汎用性の高いシステム

09:13

水耕:栄養アベイラビリティと毒性元素への曝露に栄養の割り当てと植物の応答を研究するための汎用性の高いシステム

Related Videos

32.8K Views

植物/微生物の分子相互作用およびシグナル伝達の同時および系統的分析のための水耕栽培共生システム

11:16

植物/微生物の分子相互作用およびシグナル伝達の同時および系統的分析のための水耕栽培共生システム

Related Videos

14.8K Views

抱合の単細胞藻類の栄養文化の確立

10:53

抱合の単細胞藻類の栄養文化の確立

Related Videos

14.3K Views

無菌状態で小さな分子に対する植物の反応を評価するための柔軟な低コスト化の養液栽培システム

11:27

無菌状態で小さな分子に対する植物の反応を評価するための柔軟な低コスト化の養液栽培システム

Related Videos

11.6K Views

浮遊水耕システムにおけるアラビドプシス・タリアナ根の細菌の植民地化と維持のモニタリング

09:04

浮遊水耕システムにおけるアラビドプシス・タリアナ根の細菌の植民地化と維持のモニタリング

Related Videos

11.5K Views

植物の根系アーキテクチャ特性をマッピングするための簡単なプロトコル

11:09

植物の根系アーキテクチャ特性をマッピングするための簡単なプロトコル

Related Videos

3.9K Views

半水耕栽培の根滲出液プロファイリングのための汎用性の高いガラスジャーシステム

06:33

半水耕栽培の根滲出液プロファイリングのための汎用性の高いガラスジャーシステム

Related Videos

2.8K Views

高吸水性ポリマー系独立栄養系におけるアーバスキュラー菌根培養物の接種と観察

06:43

高吸水性ポリマー系独立栄養系におけるアーバスキュラー菌根培養物の接種と観察

Related Videos

2.9K Views

JoVE logo
Contact Us Recommend to Library
Research
  • JoVE Journal
  • JoVE Encyclopedia of Experiments
  • JoVE Visualize
Business
  • JoVE Business
Education
  • JoVE Core
  • JoVE Science Education
  • JoVE Lab Manual
  • JoVE Quizzes
Solutions
  • Authors
  • Teaching Faculty
  • Librarians
  • K12 Schools
  • Biopharma
About JoVE
  • Overview
  • Leadership
Others
  • JoVE Newsletters
  • JoVE Help Center
  • Blogs
  • JoVE Newsroom
  • Site Maps
Contact Us Recommend to Library
JoVE logo

Copyright © 2026 MyJoVE Corporation. All rights reserved

Privacy Terms of Use Policies
WeChat QR code