JoVE Journal

Biochemistry

JoVE Biochemistry includes methods and experimental approaches to study the structure, function, and interactions of biological molecules.

1 2 3 4 5 6 7 8 9…16 › 361 VIDEO ARTICLES

Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays

Judith A. Stolwijk¹, Anne-Kathrin Mildner¹, Christian Kade¹, Michael Skiba¹, Guenther Bernhardt², Armin Buschauer², Harald Huebner³, Peter Gmeiner³, Joachim Wegener^1,4

¹Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, ²Institute of Pharmacy, University of Regensburg, ³Department of Chemistry and Pharmacy, Friedrich-Alexander University Erlangen-Nuernberg, ⁴Fraunhofer Research Institution for Microsystems and Solid State Technologies EMFT

JoVE 60686

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

Sen Takeda¹, Kentaro Yoshimura¹, Hiroshi Tanihata²

¹Department of Anatomy and Cell Biology, University of Yamanashi School of Medicine, ²Shimadzu Corporation

JoVE 59942

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

Yibing Deng¹, Bin Zheng¹, Yutong Liu¹, Shengchao Shi¹, Jingyuan Nie¹, Tao Wu¹, Peng Zheng¹

¹State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University

JoVE 60774

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

Jillian Rosenberg¹, Jun Huang^1,2

¹Committee on Cancer Biology, The University of Chicago, ²Pritzker School of Molecular Engineering, The University of Chicago

JoVE 59914

A Rapidly Incremented Tethered-Swimming Maximal Protocol for Cardiorespiratory Assessment of Swimmers

Dalton M. Pessôa Filho*^1,2, Danilo A. Massini*², Leandro O. C. Siqueira*², Luiz Gustavo A. Santos*², Camila M. T. Vasconcelos*², Tiago A. F. Almeida*^1,3, Mário A. C. Espada*^4,5, Joana F. Reis*^3,6, Francisco B. Alves*³, Fred J. DiMenna*^7,8

¹Department of Physical Education, São Paulo State University (UNESP) at Bauru, ²Institute of Bioscience, Graduate Program in Human Development and Technology, São Paulo State University (UNESP) at Rio Claro, ³Ciper, Faculdade de Motricidade Humana, Universidade de Lisboa, ⁴Department of Science and Technology, School of Education, Polytechnic Institute of Setúbal, ⁵Quality of Life Research Center, Polytechnic Institute of Santarem, ⁶Universidade Europeia at Lisbon, ⁷Division of Endocrinology, Diabetes and Bone Disease, Icahn School of Medicine at Mount Sinai, ⁸Department of Biobehavioral Sciences, Teachers College, Columbia University

JoVE 60630

Using an Extracellular Flux Analyzer to Measure Changes in Glycolysis and Oxidative Phosphorylation during Mouse Sperm Capacitation

Melanie Balbach¹, Jochen Buck¹, Lonny R. Levin¹

¹Department of Pharmacology, Weill Cornell Medical College

JoVE 60815

Induction of Eryptosis in Red Blood Cells Using a Calcium Ionophore

Parnian Bigdelou¹, Amir M. Farnoud^1,2

¹Biomedical Engineering Program, Ohio University, ²Department of Chemical and Biomolecular Engineering, Ohio University

JoVE 60659

Fully Processed Recombinant KRAS4b: Isolating and Characterizing the Farnesylated and Methylated Protein

Constance Agamasu¹, Peter Frank¹, Shelley Perkins¹, Timothy Waybright¹, Simon Messing¹, William Gillette¹, Andrew G. Stephen¹

¹NCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research

JoVE 60703

A Colorimetric Assay of Citrate Synthase Activity in Drosophila Melanogaster

Ping Wei*¹, Qihong Liu*², Wen Xue², Jiwu Wang²

¹Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Clinical Center for Diabetes, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, ²Department of Anatomy of Physiology, Shanghai Jiao Tong University School of Medicine

JoVE 59454

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Luis Gomez-Castillo*¹, Kurumi Watanabe*¹, Huayi Jiang*¹, Shoukai Kang¹, Liangcai Gu¹

¹Department of Biochemistry and Institute for Protein Design, University of Washington

JoVE 60738

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

Ahmed Ali^1,2, Yasmine Abouleila^1,2, Arno Germond¹

¹Biodynamics Research Center (BDR), RIKEN, ²Research Center, Misr International University

JoVE 60449

Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence

Christine Terryn¹, Anouck Habrant², Gabriel Paës², Corentin Spriet³

¹PICT Platform, Université de Reims Champagne-Ardenne, ²Fractionation of AgroResources and Environment (FARE) Laboratory, INRA, Université de Reims Champagne-Ardenne, ³Unité de Glycobiologie Structurale et Fonctionnelle, Université de Lille

JoVE 59925

Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles

Menaka Ariyaratne¹, Lingxiao Ge¹, Paul F. Morris¹

¹Department of Biological Sciences, Bowling Green State University

JoVE 60009

Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO

Adam H. Barczewski¹, Michael J. Ragusa¹, Dale F. Mierke¹, Maria Pellegrini¹

¹Department of Chemistry, Dartmouth College

JoVE 60339

Cell Culture on Silicon Nitride Membranes and Cryopreparation for Synchrotron X-ray Fluorescence Nano-analysis

Caroline Bissardon¹, Solveig Reymond¹, Murielle Salomé², Lionel André², Sam Bayat¹, Peter Cloetens², Sylvain Bohic^1,2

¹Inserm, UA7, Synchrotron Radiation for Biomedicine (STROBE), Université Grenoble Alpes, ²ID16A Beamline, ESRF, The European Synchrotron

JoVE 60461

Rapid Collection of Floral Fragrance Volatiles using a Headspace Volatile Collection Technique for GC-MS Thermal Desorption Sampling

Haleigh A. Ray¹, Charles J. Stuhl², Jennifer L. Gillett-Kaufman¹

¹Entomology and Nematology Department, University of Florida, ²Center for Medical, Agricultural and Veterinary Entomology, Agricultural Research Service U.S. Department of Agriculture

JoVE 58928

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta

Joanna Kud¹, Wenjie Wang², Yulin Yuan², Allan Caplan², Joseph C. Kuhl², Louise-Marie Dandurand¹, Fangming Xiao²

¹Department of Entomology, Plant Pathology and Nematology, University of Idaho, ²Department of Plant Sciences, University of Idaho

JoVE 60533

Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors

Keni Vidilaseris*¹, Niklas G. Johansson*², Ainoleena Turku², Alexandros Kiriazis², Gustav Boije af Gennäs², Jari Yli-Kauhaluoma², Henri Xhaard², Adrian Goldman^1,3

¹Research Program in Molecular and Integrative Biosciences, University of Helsinki, ²Drug Research Program, Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, ³School of Biomedical Sciences and Astbury Centre for Structural Molecular Biology, University of Leeds

JoVE 60619

Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling

Steven E. Guard¹, Christopher C. Ebmeier¹, William M. Old^1,2

¹Department of Molecular, Cellular and Developmental Biology, University of Colorado, ²Linda Crnic Institute for Down Syndrome, University of Colorado School of Medicine

JoVE 60432

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

David André Coucheron¹, Øystein Ivar Helle¹, Cristina Ionica Øie², Jean-Claude Tinguely¹, Balpreet Singh Ahluwalia¹

¹Department of Physics and Technology, UiT The Arctic University of Norway, ²Vascular Biology Research Group, Department of Medical Biology, UiT The Arctic University of Norway

JoVE 60378

Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase

Ryan K. Messer¹, Miguel A. Lopez Jr.¹, Gayan Senavirathne¹, Kristine E. Yoder¹

¹Cancer Biology and Genetics, College of Medicine, The Ohio State University

JoVE 59599

Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations

Mirna Swayden¹, Aurélie Dobric¹, Yolande Berthois¹, Hugo Villalba¹, Stéphane Audebert¹, Luc Camoin¹, Juan Iovanna¹, Philippe Soubeyran¹

¹Aix-Marseille Univ, INSERM, CNRS, Institut Paoli-Calmettes, CRCM

JoVE 60402

PeptiQuick, a One-Step Incorporation of Membrane Proteins into Biotinylated Peptidiscs for Streamlined Protein Binding Assays

James W. Saville¹, Lucy Troman², Franck Duong Van Hoa¹

¹Department of Biochemistry, University of British Columbia, ²School of Biochemistry, University of Bristol

JoVE 60661

SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer

Fernando Lopitz-Otsoa¹, Teresa C Delgado¹, Sofía Lachiondo-Ortega¹, Mikel Azkargorta², Felix Elortza², Manuel S Rodríguez³, María Luz Martínez-Chantar¹

¹Liver Disease and Liver Metabolism Lab, CIC bioGUNE, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), ²Proteomics Platforms, CIC bioGUNE, ProteoRED-ISCIII, CIBERehd, ³Advanced Technology Institute in Life Sciences (ITAV)-CNRS-IPBS, UbiCARE

JoVE 60098

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