JoVE Journal

Biochemistry

JoVE Biochemistry includes methods and experimental approaches to study the structure, function, and interactions of biological molecules.

1 2 3 4 5…8 › 182 VIDEO ARTICLES

Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques

June Ereño-Orbea*¹, Taylor Sicard*^1,2, Hong Cui¹, Indira Akula¹, Jean-Philippe Julien^1,2,3

¹Program in Molecular Medicine, The Hospital for Sick Children Research Institute, ²Department of Biochemistry, University of Toronto, ³Department of Immunology, University of Toronto

Assessing the Viability of a Synthetic Bacterial Consortium on the In Vitro Gut Host-microbe Interface

Marta Calatayud Arroyo¹, Tom Van de Wiele¹, Emma Hernandez-Sanabria¹

¹Center for Microbial Ecology and Technology (CMET), Ghent University

Purification and Reconstitution of TRPV1 for Spectroscopic Analysis

Francisco J. Sierra-Valdez¹, Richard A. Stein², Phanindra Velissety^1,3, Valeria Vasquez¹, Julio F. Cordero-Morales¹

¹Department of Physiology, University of Tennessee Health Science Center, ²Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, ³CuriRX, Inc.

In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria

Eveline Brodl¹, Johannes Niederhauser¹, Peter Macheroux¹

¹Institute of Biochemistry, Graz University of Technology

Generation of Native, Untagged Huntingtin Exon1 Monomer and Fibrils Using a SUMO Fusion Strategy

Andreas Reif¹, Anass Chiki¹, Jonathan Ricci¹, Hilal A. Lashuel¹

¹Laboratory of Molecular and Chemical Biology of Neurodegeneration, Ecole Polytechnique Fédérale de Lausanne (EPFL)

Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H⁺

Ya-Min Du¹, Shen-Li Zheng¹, Li Liu¹, Josef Voglmeir¹, Gabriel Yedid²

¹Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, ²College of Life Science, Nanjing Agricultural University

Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast

Anita Carija¹, Salvador Ventura¹, Susanna Navarro¹

¹Institut de Biotecnologia i Biomedicina and Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues

Dana M. Freund¹, Katherine A. Sammons², Nokwanda P. Makunga³, Jerry D. Cohen¹, Adrian D. Hegeman²

¹Department of Horticultural Science, Microbial and Plant Genomics Institute, University of Minnesota, ²Department of Horticultural Science, Department of Plant and Microbial Biology, Microbial and Plant Genomics Institute, University of Minnesota, ³Department of Botany and Zoology, Stellenbosch University

Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue

David Jelinek^1,2, Aimee Flores^1,3, Melanie Uebelhoer¹, Vincent Pasque², Kathrin Plath^2,3, M. Luisa Iruela-Arispe^1,3, Heather R. Christofk^2,3, William E. Lowry^1,3, Hilary A. Coller^1,2,3

¹Department of Molecular, Cell and Developmental Biology, UCLA, ²Department of Biological Chemistry, David Geffen School of Medicine, ³Molecular Biology Institute Interdepartmental Program, UCLA

Fluorescence Live-cell Imaging of the Complete Vegetative Cell Cycle of the Slow-growing Social Bacterium Myxococcus xanthus

Dominik Schumacher¹, Lotte Søgaard-Andersen¹

¹Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Kang-Yi Su*^1,2, Steven D. Goodman*³, Hung-Ming Lai¹, Rong-Syuan Yen¹, Wei-Yao Hu¹, Wern-Cherng Cheng², Liang-In Lin^1,2, Ya-Chien Yang^1,2, Woei-Horng Fang^1,2

¹Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, ²Department of Laboratory Medicine, National Taiwan University Hospital, ³Center for Microbial Pathogenesis, Nationwide Children's Hospital and the Department of Pediatrics, The Ohio State University

Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry

Rosi Fassler*¹, Nufar Edinger*¹, Oded Rimon¹, Dana Reichmann¹

¹Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, Safra Campus Givat Ram, The Hebrew University of Jerusalem

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

Tabitha A. Peterson¹, Mark A. Stamnes¹, Robert C. Piper¹

¹Molecular Physiology and Biophysics, University of Iowa

An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C

Cuiping Li¹, Weiyi Lai¹, Hailin Wang¹

¹State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences

The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes

Hiroyuki Yamamoto¹, Yoshikazu Sawaguchi², Michio Kimura¹

¹Department of Microbiology and Molecular Cell Biology, Nihon Pharmaceutical University, ²Department of Clinical Pharmaceutics, Nihon Pharmaceutical University

A11-positive β-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis

Huang Chunhui^1,2, Xu Dilin¹, Zhang Ke¹, Shentu Jieyi², Yan Sicheng¹, Wu Dapeng³, Wang Qinwen¹, Cui Wei^1,2

¹Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, ²Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, Ningbo University, ³School of Materials Science and Chemical Engineering, Ningbo University

Toeprinting Analysis of Translation Initiation Complex Formation on Mammalian mRNAs

Joseph A. Ross¹, Nehal Thakor^1,2,3

¹Department of Chemistry and Biochemistry, Alberta RNA Research and Training Institute, University of Lethbridge, ²Department of Neuroscience and the Canadian Centre for Behavioral Neuroscience (CCBN), University of Lethbridge, ³Arnie Charbonneau Cancer Institute, University of Calgary

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Kotaro Hama¹, Yuko Fujiwara¹, Kazuaki Yokoyama¹

¹Faculty of Pharmaceutical Sciences, Teikyo University

Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes

Shannon C. Kelly*¹, Amanda M. Eccardt*¹, Jonathan S. Fisher¹

¹Department of Biology, Saint Louis University

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Heying Cui*¹, Kyle M. Loftus*¹, Crystal R. Noell¹, Sozanne R. Solmaz¹

¹Department of Chemistry, State University of New York at Binghamton

Methods Collection

Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells

Joshua T. Maxwell¹, Chin-Hsien Tsai¹, Tahmina A. Mohiuddin², Jennifer Q. Kwong¹

¹Department of Pediatrics, Emory University School of Medicine, ²Emory College of Arts and Sciences, Emory University

Use of Two Dimensional Semi-denaturing Detergent Agarose Gel Electrophoresis to Confirm Size Heterogeneity of Amyloid or Amyloid-like Fibers

Sarah Hanna-Addams¹, Zhigao Wang¹

¹Department of Molecular Biology, UT Southwestern Medical Center

Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment

Isabel M. Schopp^1,2, Julien Béthune^1,2

¹Cluster of excellence CellNetworks, Heidelberg University, ²Heidelberg University Biochemistry Center (BZH)

Fatty Acid ¹³C Isotopologue Profiling Provides Insight into Trophic Carbon Transfer and Lipid Metabolism of Invertebrate Consumers

Ralph Menzel¹, Rainer Nehring¹, Dilara Simsek¹, Liliane Ruess¹

¹Ecology, Institute of Biology, Humboldt-Universität zu Berlin

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