JoVE Journal

Biochemistry

JoVE Biochemistry includes methods and experimental approaches to study the structure, function, and interactions of biological molecules.

‹1 2 3 4 5 6 7 8 9…12 › 271 VIDEO ARTICLES

Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling

Ryan C. Oliver¹, Swe-Htet Naing², Kevin L. Weiss¹, Sai Venkatesh Pingali¹, Raquel L. Lieberman², Volker S. Urban¹

¹Neutron Scattering Division, Oak Ridge National Laboratory, ²School of Chemistry and Biochemistry, Georgia Institute of Technology

JoVE 57901

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay

Sofiia Karchugina¹, Jonathan Chernoff¹

¹Fox Chase Cancer Center

JoVE 57755

Methods Collection

Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics

Imen Bouchnak¹, Lucas Moyet¹, Daniel Salvi¹, Marcel Kuntz¹, Norbert Rolland¹

¹Université Grenoble Alpes, INRA, CNRS, CEA

JoVE 58581

Methods Collection

Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy

Rongbing Huang*^1,2, Mengting Han*^1,2, Liying Meng^1,3, Xing Chen^1,2,3,4,5

¹College of Chemistry and Molecular Engineering, Peking University, ²Beijing National Laboratory for Molecular Sciences, Peking University, ³Peking-Tsinghua Center for Life Sciences, Peking University, ⁴Synthetic and Functional Biomolecules Center, Peking University, ⁵Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University

JoVE 58580

Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans

Sumino Yanase^1,2, Kayo Yasuda³, Naoaki Ishii³

¹Department of Health Science, School of Sports & Health Science, Daito Bunka University, ²Department of Molecular Life Science, School of Medicine, Tokai University, ³Department of Health Management, School of Health Study, Tokai University

JoVE 57807

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase

Chayan Bhattacharya*¹, Aninda Sundar Dey*¹, Navid J. Ayon*¹, William G. Gutheil¹, Mridul Mukherji¹

¹Division of Pharmaceutical Sciences, School of Pharmacy, University of Missouri-Kansas City

JoVE 57798

An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity

Antonio E. Chambers*¹, Adam E. Richardson*¹, David F. Read², Thomas J. Waller³, Douglas A. Bernstein¹, Philip J. Smaldino¹

¹Department of Biology, Ball State University, ²Department of Genome Sciences, University of Washington, ³Department of Molecular, Cellular, and Developmental Biology, University of Michigan

JoVE 58100

Rapid Isolation of the Mitoribosome from HEK Cells

Shintaro Aibara¹, Juni Andréll¹, Vivek Singh¹, Alexey Amunts¹

¹Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University

JoVE 57877

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Stacy N. Uchendu¹, Angelika Rafalowski¹, Erin F. Cohn¹, Luke W. Davoren¹, Erika A. Taylor¹

¹Department of Chemistry, Wesleyan University

JoVE 58307

Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions

Roberto Pech-Kú¹, J. Armando Muñoz-Sánchez¹, Miriam Monforte-González¹, Felipe Vázquez-Flota¹, Beatriz A. Rodas-Junco², S.M. Teresa Hernández-Sotomayor¹

¹Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, ²CONACYT, Facultad de Ingeniería Química, Campus de Ciencias Exactas e Ingeniería, Universidad Autónoma de Yucatán

JoVE 58166

Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays

Anthony Asher¹, Iniyan Ganesan¹, Laura Klasek¹, Steven M. Theg¹

¹Department of Plant Biology, University of California - Davis

JoVE 58393

Methods Collection

Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis

Marjaana Rantala¹, Virpi Paakkarinen¹, Eva-Mari Aro¹

¹Molecular Plant Biology, Department of Biochemistry, University of Turku

JoVE 58369

Methods Collection

Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free Xenopus Egg Extracts

Ye Guan^1,2, Shiyuan Wang¹, Minjun Jin², Haotian Xu³, Qiong Yang^1,4

¹Department of Biophysics, University of Michigan, Ann Arbor, ²Department of Chemistry, University of Michigan, Ann Arbor, ³Department of Computer Science, Wayne State University, ⁴Department of Physics, University of Michigan, Ann Arbor

JoVE 58240

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Narendra Padhan¹

¹Uppsala University, Dept. Immunology, Genetics and Pathology, Rudbeck Laboratory, 751 85, Uppsala, Sweden

JoVE 56794

Detection of Phospholipase C Activity in the Brain Homogenate from the Honeybee

Shota Suenami^1,2, Ryo Miyazaki¹, Takeo Kubo²

¹Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, ²Department of Biological Sciences, Graduate School of Science, The University of Tokyo

JoVE 58173

Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis

Tomáš Zavřel¹, Dominik Chmelík^1,2, Maria A. Sinetova³, Jan Červený¹

¹Department of Adaptive Biotechnologies, Global Change Research Institute, Czech Academy of Sciences, ²Department of Plant Physiology, Faculty of Science, Masaryk University, ³Laboratory of Intracellular Regulation, Institute of Plant Physiology, Russian Academy of Sciences

JoVE 58076

A Colorimetric Method for Measuring Iron Content in Plants

Jonas C. Gitz^1,2, Noy Sadot¹, Michele Zaccai¹, Raz Zarivach^1,2

¹Department of Life Sciences, Ben-Gurion University of the Negev, ²National Institute for Biotechnology in the Negev (NIBN)

JoVE 57408

Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion

Miroslava Stastna^1,2,3, Amandine Thomas¹, Juliana Germano¹, Somayeh Pourpirali¹, Jennifer E. Van Eyk^1,2, Roberta A. Gottlieb¹

¹The Smidt Heart Institute, Cedars-Sinai Medical Center, ²Advanced Clinical Biosystems Research Institute, Cedars-Sinai Medical Center, ³Institute of Analytical Chemistry of the Czech Academy of Sciences

JoVE 58079

A High-Throughput Luciferase Assay to Evaluate Proteolysis of the Single-Turnover Protease PCSK9

John S. Chorba¹, Adri M. Galvan², Kevan M. Shokat²

¹Division of Cardiology, Department of Medicine, Zuckerberg San Francisco General and University of California San Francisco, ²Department of Cellular and Molecular Pharmacology and Howard Hughes Medical Institute, University of California San Francisco

JoVE 58265

Isolating and Incorporating Light-Harvesting Antennas from Diatom Cyclotella Meneghiniana in Liposomes with Thylakoid Lipids

Kerstin Pieper¹, Kathi Gundermann¹, Lars Dietzel¹

¹Institute for Molecular Biosciences, Goethe University

JoVE 58017

Genetic Incorporation of Biosynthesized L-dihydroxyphenylalanine (DOPA) and Its Application to Protein Conjugation

Sanggil Kim¹, Hyun Soo Lee¹

¹Department of Chemistry, Sogang University

JoVE 58383

Plate-based Large-scale Cultivation of Caenorhabditis elegans: Sample Preparation for the Study of Metabolic Alterations in Diabetes

Katharina Kohl¹, Thomas Fleming^2,3, Kübra Acunman¹, Hans-Peter Hammes^1,4, Michael Morcos*¹, Andrea Schlotterer*¹

¹5th Medical Department, Medical Faculty Mannheim, Heidelberg University, ²Department of Internal Medicine, Heidelberg University, ³German Center for Diabetes Research (DZD), ⁴European Center for Angioscience, Medical Faculty Mannheim, Heidelberg University

JoVE 58117

Functional Characterization of Carboxylesterases in Insecticide Resistant House Flies, Musca Domestica

Xuechun Feng¹, Nannan Liu¹

¹Department of Entomology and Plant Pathology, Auburn University

JoVE 58106

Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory

Keri L Colabroy¹, Katlyn Mayer¹

¹Department of Chemistry, Muhlenberg College

JoVE 58012

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