JoVE Journal

Biochemistry

JoVE Biochemistry includes methods and experimental approaches to study the structure, function, and interactions of biological molecules.

‹1 2 3 4 5 6 7 8 9…19 › 436 VIDEO ARTICLES

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

Santosh Adhikari¹, Chiranjib Banerjee*¹, Joe Moscatelli*^1,2, Elias M. Puchner¹

¹School of Physics and Astronomy, University of Minnesota, Twin Cities, Physics and Nanotechnology (PAN), ²Middlebury College

JoVE 60950

Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay

Emily M. Chester¹, Emily Fender¹, Michael D. Wasserman¹

¹Department of Anthropology, Indiana University

JoVE 61005

A Planarian Motility Assay to Gauge the Biomodulating Properties of Natural Products

Evelyn B. Voura¹, Carlos H. Pulquerio¹, Robyn A. M. V. Fong¹, Zaina Imani¹, Pablo J. Rojas¹, Angela M. Pratt¹, Nicole M. Shantel¹, Elisa J. Livengood¹

¹State University of New York (SUNY) at Morrisville

JoVE 61070

Methods Collection

An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants

Hannes Steinkellner¹, Alexander V. Beribisky¹, Philip Mausberg¹, John Christodoulou², Barbara Scheiber-Mojdehkar³, Anna Huber¹, Victoria Sarne¹, Franco Laccone¹

¹Institute of Medical Genetics, Center for Pathobiochemistry and Genetics, Medical University of Vienna (MUV), ²Murdoch Children's Research Institute and Department of Paediatrics, University of Melbourne, Discipline of Child & Adolescent Health, Sydney Medical School, ³Department of Medical Chemistry and Pathobiochemistry, Medical University of Vienna

JoVE 61054

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Vito M. Butardo Jr.¹, Christiane Seiler², Nese Sreenivasulu³, Markus Kuhlmann²

¹Department of Chemistry and Biotechnology, Swinburne University of Technology, ²Department of Molecular Genetics, Heterosis Research Group, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), ³Grain Quality and Nutrition Center, Applied Functional Genomics Cluster, International Rice Research Institute

JoVE 60602

Deciphering the Structural Effects of Activating EGFR Somatic Mutations with Molecular Dynamics Simulation

Mahlet Z. Tamirat¹, Kari J. Kurppa², Klaus Elenius^2,3,4, Mark S. Johnson¹

¹Structural Bioinformatics Laboratory, Biochemistry, Faculty of Science and Engineering, Åbo Akademi University, ²Medicity Research Laboratories and Institute of Biomedicine, University of Turku, ³Turku Bioscience Centre, University of Turku and Åbo Akademi University, ⁴Department of Oncology and Radiotherapy, University of Turku and Turku University Hospital

JoVE 61125

X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050

Raphael Dutoit^1,2, Nathalie Brandt², Dany Van Elder¹, Louis Droogmans¹

¹Laboratory of Microbiology, Department of Molecular Biology, Université Libre de Bruxelles, ²Labiris Institut de Recherche

JoVE 61288

Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening

Karine Nozeret¹, Aurélia Pernin¹, Nienke Buddelmeijer¹

¹Institut Pasteur, Unité Biologie et génétique de la paroi bactérienne, Paris, France ; CNRS, UMR 2001 « Microbiologie intégrative et Moléculaire », Paris, France ; INSERM, Équipe Avenir, Paris, France

JoVE 61146

Monitoring Protein-RNA Interaction Dynamics in vivo at High Temporal Resolution using χCRAC

Stuart W. McKellar¹, Ivayla Ivanova¹, Robert W. van Nues², Ross A. Cordiner³, Will Worboys⁴, Andrew Langford⁴, Torben Heick Jensen³, Sander Granneman¹

¹Centre for Synthetic and Systems Biology, University of Edinburgh, ²Institute of Cell Biology, University of Edinburgh, ³Department of Molecular Biology and Genetics, Aarhus University, ⁴UVO3 Ltd.

JoVE 61027

Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates

Monica C. Pillon¹, Robin E. Stanley¹

¹Signal Transduction Laboratory, National Institute of Environmental Health Sciences, Department of Health and Human Services, National Institutes of Health

JoVE 61258

A Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) Platform for Investigating Peptide Biosynthetic Enzymes

Yeganeh Habibi¹, Christopher J. Thibodeaux¹

¹Department of Chemistry, McGill University

JoVE 61053

Methods Collection

Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes

Dohwan Lee*¹, Kyung Wook Wee*¹, Hyerin Kim*¹, Sung Il Han¹, Seungmin Kwak^1,2, Dae Sung Yoon³, Jeong Hoon Lee¹

¹Department of Electrical Engineering, Kwangwoon University, ²Korea Institute of Science and Technology (KIST), ³School of Biomedical Engineering, Korea University

JoVE 61292

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation

Qin Fu¹, Casey W. Johnson¹, Bhagya K. Wijayawardena², Michael P. Kowalski², Miranda Kheradmand², Jennifer E. Van Eyk¹

¹Advanced Clinical Biosystems Institute, Smidt Heart institute, Cedars Sinai Medical Center, ²Beckman Coulter Life Sciences

JoVE 59842

Detection of Protein S-Acylation using Acyl-Resin Assisted Capture

Ritika Tewari*¹, Savannah J. West*^1,2, Bieerkehazi Shayahati¹, Askar M. Akimzhanov¹

¹Department of Biochemistry and Molecular Biology, McGovern Medical School at UT Health, ²MD Anderson UT Health Graduate School

JoVE 61016

Methods Collection

In Vivo Hydroxyl Radical Protein Footprinting for the Study of Protein Interactions in Caenorhabditis elegans

Jessica A. Espino¹, Lisa M. Jones¹

¹Department of Pharmaceutical Sciences, University of Maryland

JoVE 60910

Methods Collection

Acyl-PEGyl Exchange Gel Shift Assay for Quantitative Determination of Palmitoylation of Brain Membrane Proteins

David J. Speca¹, Elva Diaz¹

¹Department of Pharmacology, UC Davis School of Medicine

JoVE 61018

Methods Collection

Characterization of Amyloid Structures in Aging C. Elegans Using Fluorescence Lifetime Imaging

Maria Lucia Pigazzini*^1,2, Christian Gallrein*¹, Manuel Iburg*¹, Gabriele Kaminski Schierle³, Janine Kirstein^1,4

¹Leibniz Research Institute for Molecular Pharmacology im Forschungsverbund Berlin, ²NeuroCure Cluster of Excellence, Charité - Universitätsmedizin Berlin, ³Molecular Neuroscience Group, Department of Chemical Engineering and Biotechnology, University of Cambridge, ⁴Cell Biology, University of Bremen

JoVE 61004

Methods Collection

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol

Alexandria Slayden¹, Kelsey North¹, Shivantika Bisen¹, Alex M. Dopico¹, Anna N. Bukiya¹, Avia Rosenhouse-Dantsker²

¹Department of Pharmacology, Addiction Science and Toxicology, The University of Tennessee HSC, ²Department of Chemistry, University of Illinois at Chicago

JoVE 60734

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

Karel Bezstarosti¹, Lennart van der Wal¹, Jeroen A. A. Demmers¹

¹Proteomics Center, Erasmus University Medical Center

JoVE 59079

Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson's Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation

Ying Fan*¹, Francesca Tonelli*¹, Shalini Padmanabhan², Marco A.S. Baptista², Lindsey Riley², Danielle Smith³, Connie Marras⁴, Andrew Howden⁵, Dario R. Alessi¹, Esther Sammler^1,6

¹MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, ²The Michael J. Fox Foundation for Parkinson's Research, ³Department of Medical and Molecular Genetics, Indiana University School of Medicine, ⁴Morton and Gloria Shulman Movement Disorders Centre and the Edmond J. Safra Program in Parkinson's Disease, Toronto Western Hospital, University of Toronto, ⁵Division of Cell Signalling and Immunology, School of Life Sciences, University of Dundee, ⁶Division of Molecular and Clinical Medicine, School of Medicine, Ninewells Hospital and Medical School, University of Dundee

JoVE 58956

Strategic Screening and Characterization of the Visual GPCR-mini-G Protein Signaling Complex for Successful Crystallization

Filip Pamula^1,2, Jonas Mühle¹, Alain Blanc³, Rony Nehmé⁴, Patricia C. Edwards⁴, Christopher G. Tate⁴, Ching-Ju Tsai¹

¹Laboratory of Biomolecular Research, Paul Scherrer Institute, ²Department of Biology, ETH Zürich, ³Center for Radiopharmaceutical Sciences, Paul Scherrer Institute, ⁴Laboratory of Molecular Biology, Medical Research Council

JoVE 60747

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

Hayley Abbiss^1,2,3, Joel P. A. Gummer^1,4, Michael Francki^5,6, Robert D. Trengove¹

¹Research and Innovation, Murdoch University, ²Centre for Digital Agriculture, Curtin University, ³Centre for Integrative Metabolomics and Computational Biology, School of Science, Edith Cowan University, ⁴Centre for Computational and System Medicine, Health Futures Institute, Murdoch University, ⁵Department of Primary Industries and Regional Development, ⁶State Agricultural Biotechnology Centre, Murdoch University

JoVE 60851

Visualization of Estrogen Receptors in Colons of Mice with TNBS-Induced Crohn's Disease using Immunofluorescence

Damian Jacenik¹, Marta Zielińska², Sylwia Michlewska³, Jakub Fichna², Wanda M. Krajewska¹

¹Department of Cytobiochemistry, Faculty of Biology and Environmental Protection, University of Lodz, ²Department of Biochemistry, Faculty of Medicine, Medical University of Lodz, ³Laboratory of Microscopic Imaging and Specialized Biological Techniques, Faculty of Biology and Environmental Protection, University of Lodz

JoVE 60813

Characterizing Cellular Proteins with In-cell Fast Photochemical Oxidation of Proteins

Emily E. Chea¹, Aimee Rinas², Jessica A. Espino¹, Lisa M. Jones¹

¹Department of Pharmaceutical Sciences, University of Maryland Baltimore, ²AIT Bioscience

JoVE 60911

Methods Collection

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