JoVE Journal

Biochemistry

JoVE Biochemistry includes methods and experimental approaches to study the structure, function, and interactions of biological molecules.

‹1 2 3 4 5 6 7 8 9…17 › 386 VIDEO ARTICLES

SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer

Fernando Lopitz-Otsoa¹, Teresa C Delgado¹, Sofía Lachiondo-Ortega¹, Mikel Azkargorta², Felix Elortza², Manuel S Rodríguez³, María Luz Martínez-Chantar¹

¹Liver Disease and Liver Metabolism Lab, CIC bioGUNE, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), ²Proteomics Platforms, CIC bioGUNE, ProteoRED-ISCIII, CIBERehd, ³Advanced Technology Institute in Life Sciences (ITAV)-CNRS-IPBS, UbiCARE

JoVE 60098

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Alien Balian*^1,2,3, Javier Garcia Gonzalez*^1,2,3, Nora Bastida^1,3, Khadija-Tul Kubra Akhtar^1,3, Baris A. Borsa^1,2,3, Frank J. Hernandez^1,2,3

¹Department of Physics, Chemistry and Biology, Linköping University, ²Wallenberg Centre for Molecular Medicine (WCMM), ³Nucleic Acids Technologies Laboratory (NAT-lab), Linköping University

JoVE 60005

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry

Michael Vilkhovoy¹, David Dai¹, Sandra Vadhin*¹, Abhinav Adhikari*¹, Jeffrey D. Varner¹

¹Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University

JoVE 60329

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays

Fernanda Festa^1,2, Joshua Labaer¹

¹Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, ²Department of Pediatrics, and Biochemistry/Molecular Biology, College of Medicine, Pennsylvania State University

JoVE 59886

Methods Collection

In Vivo Calcium Imaging in C. elegans Body Wall Muscles

Ashley A. Martin^1,2, Simon Alford³, Janet E. Richmond¹

¹Department of Biological Sciences, University of Illinois at Chicago, ²Department of Integrative Biology and Physiology, University of Minnesota, ³Department of Anatomy and Cell Biology, University of Illinois at Chicago

JoVE 59175

Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation

Jingjie Hu¹, Nathan D. Derr^1,2

¹Department of Biological Sciences, Smith College, ²Center for Microscopy and Imaging, Smith College

JoVE 60369

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

Catrin Swantje Müller¹, Wolfgang Bildl¹, Norbert Klugbauer², Alexander Haupt¹, Bernd Fakler^1,3, Uwe Schulte^1,3,4

¹Institute of Physiology, Faculty of Medicine, University of Freiburg, Freiburg, Germany, ²Institute of Experimental and Clinical Pharmacology and Toxicology, Faculty of Medicine, University of Freiburg, Freiburg, Germany, ³Signalling Research Centres BIOSS and CIBSS, Freiburg, Germany, ⁴Logopharm GmbH, Germany

JoVE 60096

Examining the Dynamics of Cellular Adhesion and Spreading of Epithelial Cells on Fibronectin During Oxidative Stress

Caitlin E Tolbert¹, Lindsey Palmquist², Hannah Lee Dixon², Melissa C. Srougi^2,3

¹Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill, ²Department of Chemistry, High Point University, ³Biotechnology Program and the Department of Molecular Biomedical Sciences, North Carolina State University

JoVE 59989

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling

Stefan Ilic¹, Shira Cohen¹, Ariel Afek², Raluca Gordan², David B. Lukatsky¹, Barak Akabayov¹

¹Department of Chemistry, Ben-Gurion University of the Negev, ²Center for Genomic and Computational Biology, Department of Biostatistics and Bioinformatics, Duke University

JoVE 59737

Perturbing Endothelial Biomechanics via Connexin 43 Structural Disruption

Md. Mydul Islam¹, Robert L. Steward, Jr.^1,2

¹Department of Mechanical and Aerospace Engineering, University of Central Florida, ²Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida

JoVE 60034

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Katherine M. Murphy¹, Siwon Chung¹, Shruti Fogla¹, Hana B. Minsky¹, Karen Yong Zhu¹, Philipp Zerbe¹

¹Department of Plant Biology, University of California Davis

JoVE 59992

Interactions with and Membrane Permeabilization of Brain Mitochondria by Amyloid Fibrils

Ramin Zadali¹, Ebrahim Rostampour Ghareghozloo*¹, Mohammad Ramezani*¹, Vahid Hassani*¹, Yasin Rafiei*¹, Shiva Mohammadi Chiyaneh¹, Ali Akbar Meratan¹

¹Department of Biological Sciences, Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan, 45137-66731, Iran

JoVE 59883

Quantification of Coenzyme A in Cells and Tissues

Matthew W. Frank¹, Chitra Subramanian¹, Charles O. Rock¹, Suzanne Jackowski¹

¹Department of Infectious Diseases, St. Jude Children's Research Hospital

JoVE 60182

A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput

Nasir Malik¹, Rohini Manickam¹, Muznabanu Bachani¹, Joseph P. Steiner¹

¹National Institute of Neurological Disorders and Stroke (NINDS), Neurotherapeutic Development Unit (NTDU), National Institutes of Health (NIH)

JoVE 59333

Synthesis of a Deuterated Standard for the Quantification of 2-Arachidonoylglycerol in Caenorhabditis elegans

Julia Fernández de Luco¹, Gastón Prez², Bruno Hernández Cravero², Diego de Mendoza², Guillermo R. Labadie^1,3

¹Instituto de Química Rosario (IQUIR-CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, ²Laboratorio de Fisiología Microbiana, Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, ³Departamento de Química Orgánica, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario

JoVE 59882

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy

Francesco Simone Ruggeri¹, Tomas Šneideris^1,2, Sean Chia¹, Michele Vendruscolo¹, Tuomas P. J. Knowles^1,3

¹Centre for Misfolding Diseases, Department of Chemistry, University of Cambridge, ²Institute of Biotechnology, Life Sciences Center, Vilnius University, ³Cavendish Laboratory, Department of Physics, University of Cambridge

JoVE 60108

Imaging of Extracellular Vesicles by Atomic Force Microscopy

Mikhail Skliar^1,2, Vasiliy S. Chernyshev^3,4

¹Department of Chemical Engineering, University of Utah, ²The Nano Institute of Utah, University of Utah, ³Center for Photonics and Quantum Materials, Skolkovo Institute of Science and Technology, ⁴Biopharmaceutical Cluster 'Northern', Moscow Institute of Physics and Technology

JoVE 59254

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Julian M Rocha¹, Andreas Gahlmann^1,2

¹Department of Chemistry, University of Virginia, ²Department of Molecular Physiology & Biological Physics, University of Virginia School of Medicine

JoVE 59387

A Double Humanized BLT-mice Model Featuring a Stable Human-Like Gut Microbiome and Human Immune System

Lance Daharsh^1,2, Jianshui Zhang^1,2, Amanda Ramer-Tait³, Qingsheng Li^1,2

¹Nebraska Center for Virology, ²School of Biological Sciences, University of Nebraska-Lincoln, ³Department of Food Science and Technology, University of Nebraska-Lincoln

JoVE 59773

A Semi-Quantitative Drug Affinity Responsive Target Stability (DARTS) assay for studying Rapamycin/mTOR interaction

Chen Zhang*¹, Min Cui*¹, Yazhou Cui¹, Aubryanna Hettinghouse¹, Chuan-ju Liu^1,2

¹Department of Orthopaedic Surgery, New York University Medical Center, ²Department of Cell Biology, New York University School of Medicine

JoVE 59656

Preparation of High-Quality Fermented Fish Product

Fang Yang*¹, Lu-Lu Zhu*¹, Yu-Duan Diao¹, Pei Gao¹, Da-Wei Yu¹, Pei-Pei Yu¹, Qi-Xing Jiang¹, Yan-Shun Xu¹, Wen-Shui Xia¹, Xiao-Bei Zhan²

¹State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, ²The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University

JoVE 60265

Cortisol Measurement in Koala (Phascolarctos cinereus) Fur

Renae Charalambous¹, Edward Narayan¹

¹School of Science and Health, Western Sydney University

JoVE 59216

Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil (Ers4tU)

J. David Barrass¹, Jean D. Beggs¹

¹Wellcome Centre for Cell Biology, School of Biological Sciences, University of Edinburgh

JoVE 59952

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

Emily A. Brown^1,2, Steven C. Neier^3,4, Claudia Neuhauser⁵, Adam G. Schrum^6,7,8, Stephen E.P. Smith^1,2,9

¹Center for Integrative Brain Research, Seattle Children's Research Institute, ²Graduate Program in Neuroscience, University of Washington, ³Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Department of Medicine, Harvard Medical School, ⁴Broad Institute of Harvard and MIT, ⁵Department of Mathematics, University of Houston, ⁶Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri, ⁷Department of Surgery, School of Medicine, University of Missouri, ⁸Department Bioengineering, College of Engineering, University of Missouri, ⁹Department of Pediatrics, University of Washington

JoVE 60029

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