JoVE Journal

Biochemistry

JoVE Biochemistry includes methods and experimental approaches to study the structure, function, and interactions of biological molecules.

‹1 2 3 4 5 6 7 8 9…14 › 334 VIDEO ARTICLES

Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In vivo

Patrick Moran^1,2, Yichen Guo^3,4, Rong Yuan^1,3, Nicholas Barnekow¹, Jordan Palmer², Adam Beck³, Bin Ren^3,4,5

¹Blood Research Institute, Blood Center of Wisconsin, ²Department of Medicine, Medical College of Wisconsin, ³Department of Surgery, The University of Alabama at Birmingham, ⁴Department of Biomedical Engineering, The University of Alabama at Birmingham, ⁵GBS Program, Graduate School, The University of Alabama at Birmingham

JoVE 59624

Hybrid Clear/Blue Native Electrophoresis for the Separation and Analysis of Mitochondrial Respiratory Chain Supercomplexes

Alexanne Cuillerier¹, Yan Burelle¹

¹Interdisciplinary School of Health Science, Faculty of Health Sciences and Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa

JoVE 59294

Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells

Shellaina J.V. Gordon*¹, Yao Xiao*², Amanda L. Paskavitz³, Napoleón Navarro-Tito⁴, Juan G. Navea², Teresita Padilla-Benavides¹

¹Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, ²Department of Chemistry, Skidmore College, ³Candiac MR Center, Beth Israel Deaconess Medical Center, ⁴Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero

JoVE 59519

Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids

Phillip C. Delekta¹, Todd A. Lydic², Neal D. Hammer¹

¹Department of Microbiology and Molecular Genetics, Michigan State University, ²Department of Physiology, Michigan State University

JoVE 59538

Using the Open-Source MALDI TOF-MS IDBac Pipeline for Analysis of Microbial Protein and Specialized Metabolite Data

Chase M. Clark¹, Maria S. Costa^1,2, Erin Conley¹, Emma Li¹, Laura M. Sanchez¹, Brian T. Murphy¹

¹Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, ²Faculty of Pharmaceutical Sciences, University of Iceland

JoVE 59219

Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate

Markus Axmann¹, Andreas Karner², Sabine M. Meier¹, Herbert Stangl¹, Birgit Plochberger²

¹Center for Pathobiochemistry and Genetics, Institute of Medical Chemistry and Pathobiochemistry, Medical University Vienna, ²School of Medical Engineering and Applied Social Sciences, University of Applied Sciences Upper Austria

JoVE 59573

Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay

Ingo Bauer¹, Angelo Pidroni¹, Özgür Bayram^2,3, Gerald Brosch¹, Stefan Graessle¹

¹Division of Molecular Biology, Biocenter, Medical University of Innsbruck, ²Biology Department, Maynooth University, ³Maynooth University Human Health Research Institute

JoVE 59527

Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins

Rui Yin, Catherine Harvey, Diane C. Shakes, Oliver Kerscher

¹Integrated Science Center, Biology Department, College of William & Mary

JoVE 59576

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels

Alex Y. H. Wong¹, Sheng Xie¹, Ben Zhong Tang², Sijie Chen¹

¹Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, ²Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute of Molecular Functional Materials, State Key Laboratory of Neuroscience, Division of Biomedical Engineering, and Division of Life Science, Hong Kong University of Science and Technology

JoVE 58669

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Simon Leclerc^1,2, Youri Arntz², Yuichi Taniguchi^1,3

¹Laboratory for Cell Systems Control, RIKEN Center for Biosystems Dynamics Research, RIKEN, ²Laboratoire de Biomatériaux et Bioimagerie, INSERM, ³PRESTO, Japan Science and Technology Agency

JoVE 59199

Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors

Fatima Enam¹, Thomas J. Mansell¹

¹Department of Chemical and Biological Engineering, Iowa State University

JoVE 59253

Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting

Yu-Ting Huang^1,2, Dinja van der Hoorn*^1,2, Leire M. Ledahawsky*^1,2, Anna A. L. Motyl*^1,2, Crispin Y. Jordan¹, Thomas H. Gillingwater^1,2, Ewout J. N. Groen^1,2

¹Centre for Discovery Brain Sciences, University of Edinburgh, ²Euan MacDonald Centre for Motor Neurone Disease Research, University of Edinburgh

JoVE 59438

Ion Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization

Hadar Amartely¹, Daniel Some², Ayala Tsadok³, Mario Lebendiker¹

¹Wolfson Centre for Applied Structural Biology, The Alexander Silberman Institute of Life Science, The Hebrew University of Jerusalem, ²Wyatt Technology Corporation, ³Danyel Biotech Ltd

JoVE 59408

DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes

Casey S. O’Neil¹, Jacie L. Beach¹, Todd D. Gruber¹

¹Department of Molecular Biology and Chemistry, Christopher Newport University

JoVE 59341

Fully Autonomous Characterization and Data Collection from Crystals of Biological Macromolecules

Stephanie Hutin¹, Bart Van Laer¹, Christoph Mueller-Dieckmann¹, Gordon Leonard¹, Didier Nurizzo¹, Matthew W. Bowler²

¹Structural Biology Group, European Synchrotron Radiation Facility, ²Grenoble Outstation, European Molecular Biology Laboratory

JoVE 59032

Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect

Stephanie Hutin*¹, Gianluca Santoni*¹, Ulrich Zander², Nicolas Foos¹, Sylvain Aumonier¹, Guillaume Gotthard¹, Antoine Royant^1,3, Christoph Mueller-Dieckmann¹, Gordon Leonard¹

¹European Synchrotron Radiation Facility, Structural Biology Group, ²European Molecular Biology Laboratory, ³Univ. Grenoble Alpes, CNRS, CEA, IBS (Institut de Biologie Structurale)

JoVE 58594

Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale

Melaku Garsamo^1,3, Yun Zhou^2,3, Xing Liu^1,3

¹Department of Biochemistry, Purdue University, ²Department of Botany and Plant Pathology, Purdue University, ³Center for Plant Biology, Purdue University

JoVE 59038

Expression, Solubilization, and Purification of Eukaryotic Borate Transporters

Sebastian Flores¹, Andrew P. Feld¹, Bryan H. Thurtle-Schmidt¹

¹Department of Biology, Davidson College

JoVE 59166

Quantification of Hypopigmentation Activity In Vitro

Yeon-Ji Kim¹, Min-Jung Kim¹, Dong-Keon Kweon², Seung-Taik Lim¹, Sung-Joon Lee¹

¹Department of Biotechnology, Graduate School of Life Sciences & Biotechnology, College of Life Sciences and Biotechnology, Korea University, ²JinWoo Bio Co. Ltd

JoVE 58185

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle

Sujin Kim¹, Minjeong Kang¹, Yoosik Kim¹

¹Department of Chemical and Biomolecular Engineering and KI for Health Science and Technology (KIHST), Korea Advanced Institute of Science and Technology (KAIST)

JoVE 59215

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Zackary N. Scholl¹, Qing Li¹, Eric Josephs¹, Dimitra Apostolidou¹, Piotr E. Marszalek¹

¹Mechanical Engineering and Materials Science, Duke University

JoVE 55989

Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells

Dominik P. Buser¹, Martin Spiess¹

¹Biozentrum, University of Basel

JoVE 59111

Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach

John E. Chojnowski¹, Emily A. McMillan¹, Todd I. Strochlic¹

¹Department of Biochemistry and Molecular Biology, Drexel University College of Medicine

JoVE 59037

Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy

Ievgen Mazurenko¹, Nikos S. Hatzakis², Lars J.C. Jeuken¹

¹School of Biomedical Sciences & the Astbury Centre for Structural Molecular Biology, University of Leeds, ²Department of Chemistry and Nano-Science Center, University of Copenhagen

JoVE 58896

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