JoVE Journal

Biochemistry

JoVE Biochemistry includes methods and experimental approaches to study the structure, function, and interactions of biological molecules.

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Toeprinting Analysis of Translation Initiation Complex Formation on Mammalian mRNAs

Joseph A. Ross¹, Nehal Thakor^1,2,3

¹Department of Chemistry and Biochemistry, Alberta RNA Research and Training Institute, University of Lethbridge, ²Department of Neuroscience and the Canadian Centre for Behavioral Neuroscience (CCBN), University of Lethbridge, ³Arnie Charbonneau Cancer Institute, University of Calgary

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Kotaro Hama¹, Yuko Fujiwara¹, Kazuaki Yokoyama¹

¹Faculty of Pharmaceutical Sciences, Teikyo University

Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes

Shannon C. Kelly*¹, Amanda M. Eccardt*¹, Jonathan S. Fisher¹

¹Department of Biology, Saint Louis University

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Heying Cui*¹, Kyle M. Loftus*¹, Crystal R. Noell¹, Sozanne R. Solmaz¹

¹Department of Chemistry, State University of New York at Binghamton

Methods Collection

Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells

Joshua T. Maxwell¹, Chin-Hsien Tsai¹, Tahmina A. Mohiuddin², Jennifer Q. Kwong¹

¹Department of Pediatrics, Emory University School of Medicine, ²Emory College of Arts and Sciences, Emory University

Use of Two Dimensional Semi-denaturing Detergent Agarose Gel Electrophoresis to Confirm Size Heterogeneity of Amyloid or Amyloid-like Fibers

Sarah Hanna-Addams¹, Zhigao Wang¹

¹Department of Molecular Biology, UT Southwestern Medical Center

Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment

Isabel M. Schopp^1,2, Julien Béthune^1,2

¹Cluster of excellence CellNetworks, Heidelberg University, ²Heidelberg University Biochemistry Center (BZH)

Fatty Acid ¹³C Isotopologue Profiling Provides Insight into Trophic Carbon Transfer and Lipid Metabolism of Invertebrate Consumers

Ralph Menzel¹, Rainer Nehring¹, Dilara Simsek¹, Liliane Ruess¹

¹Ecology, Institute of Biology, Humboldt-Universität zu Berlin

Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1

Yoshihide Tokunou¹, Kazuhito Hashimoto², Akihiro Okamoto²

¹Department of Applied Chemistry, University of Tokyo, ²Global Research Center for Environment and Energy based on Nanomaterials Science, National Institute for Materials Science

Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum

Gustavo Diaz¹, Chandler Bridges¹, Megan Lucas¹, Yong Cheng², Jeff S. Schorey², Karen M. Dobos¹, Nicole A. Kruh-Garcia¹

¹Department of Microbiology, Immunology and Pathology, Colorado State University, ²Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame

Polysome Profiling in Leishmania, Human Cells and Mouse Testis

Zemfira N. Karamysheva², Elena B. Tikhonova¹, Petar N. Grozdanov¹, James C. Huffman^2,3, Kristen R. Baca^1,3, Alexander Karamyshev¹, R. Brian Denison¹, Clinton C. MacDonald¹, Kai Zhang², Andrey L. Karamyshev¹

¹Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, ²Department of Biological Sciences, Texas Tech University, ³CISER (Center for the Integration of STEM Education & Research), Texas Tech University

Measurement of Mitochondrial Oxygen Consumption in Permeabilized Fibers of Drosophila Using Minimal Amounts of Tissue

Chloé J Simard*¹, Guillaume Pelletier*¹, Luc H Boudreau¹, Etienne Hebert-Chatelain², Nicolas Pichaud¹

¹Département de chimie et biochimie, Université de Moncton, ²Département de biologie, Université de Moncton

Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.

Mikhail A. Galkin¹, Aidan N. Russell², Steven B. Vik³, Richard M. Berry², Robert R. Ishmukhametov²

¹First Pavlov State Medical University, ²Clarendon Laboratory, Department of Physics, Oxford University, ³Department of Biological Sciences, Southern Methodist University

Lipid Index Determination by Liquid Fluorescence Recovery in the Fungal Pathogen Ustilago Maydis

Lucero Romero-Aguilar*^1,2, Mónica Montero-Lomeli*³, Juan Pablo Pardo², Guadalupe Guerra- Sánchez¹

¹Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, ²Facultad de Medicina, Universidad Nacional Autónoma de México, ³Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by ³²P-postlabeling

Marie Stiborova¹

¹Department of Biochemistry, Charles University

A Simple Method for High Throughput Chemical Screening in Caenorhabditis Elegans

Mark Lucanic¹, Theo Garrett¹, Matthew S. Gill², Gordon J. Lithgow¹

¹The Buck Institute for Research on Aging, ²The Scripps Research Institute Center on Aging

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Ozlem Yaren¹, Barry W. Alto², Kevin M. Bradley³, Patricia Moussatche³, Lyudmyla Glushakova³, Steven A. Benner^1,3

¹Foundation for Applied Molecular Evolution (FfAME), ²Florida Medical Entomology Laboratory, University of Florida, ³Firebird Biomolecular Sciences LLC

Regeneration of Arrayed Gold Microelectrodes Equipped for a Real-Time Cell Analyzer

Zhihui Xu*¹, Yiyan Song*¹, Huijun Jiang², Yan Kong³, Xiaoming Li³, Jin Chen¹, Yuan Wu⁴

¹School of Public Health, Nanjing Medical University, ²School of Pharmacy, Nanjing Medical University, ³State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemistry and Chemical Engineering, Nanjing Tech University, ⁴Department of Medical Oncology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University

Detection of Detergent-sensitive Interactions Between Membrane Proteins

Nava Zaarur¹, Xiang Pan¹, Konstantin V. Kandror¹

¹Department of Biochemistry, Boston University School of Medicine

Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay

Bo Han¹, Man Zhang², Peng Sun¹, Shangwei Hou³

¹Department of General Surgery, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, ²Key Laboratory of Systems Biomedicine (Ministry of Education), Institute of Systems Biomedicine, Shanghai Jiao Tong University, ³Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine

Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation

Chloé I Charendoff¹, Lisa Bouchier-Hayes²

¹Department of Pediatrics, Division of Hematology-Oncology, Baylor College of Medicine, ²Department of Pediatrics, Division of Hematology-Oncology and Department of Molecular and Cellular Biology, Baylor College of Medicine

Fractionation for Resolution of Soluble and Insoluble Huntingtin Species

Joseph Ochaba^1,2, Eva L. Morozko^2,3, Jacqueline G. O’Rourke⁴, Leslie M. Thompson^1,2,3

¹Department of Psychiatry and Human Behavior, University of California Irvine, ²UCI MIND, University of California Irvine, ³Department of Neurobiology and Behavior, University of California Irvine, ⁴Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases

Ryan J. Mailloux¹, Adrian Young¹, Marisa O'Brien¹, Robert Morris Gill¹

¹Department of Biochemistry, Memorial University of Newfoundland

Titration ELISA as a Method to Determine the Dissociation Constant of Receptor Ligand Interaction

Johannes A. Eble¹

¹Institute of Physiological Chemistry and Pathobiochemistry, University of Münster

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