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JoVE Journal

Biochemistry

JoVE Biochemistry includes methods and experimental approaches to study the structure, function, and interactions of biological molecules.

‹1…3 4 5 6 7 8 9…18 › 420 VIDEO ARTICLES

Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO

Adam H. Barczewski¹, Michael J. Ragusa¹, Dale F. Mierke¹, Maria Pellegrini¹

¹Department of Chemistry, Dartmouth College

Cell Culture on Silicon Nitride Membranes and Cryopreparation for Synchrotron X-ray Fluorescence Nano-analysis

Caroline Bissardon¹, Solveig Reymond¹, Murielle Salomé², Lionel André², Sam Bayat¹, Peter Cloetens², Sylvain Bohic^1,2

¹Inserm, UA7, Synchrotron Radiation for Biomedicine (STROBE), Université Grenoble Alpes, ²ID16A Beamline, ESRF, The European Synchrotron

Rapid Collection of Floral Fragrance Volatiles using a Headspace Volatile Collection Technique for GC-MS Thermal Desorption Sampling

Haleigh A. Ray¹, Charles J. Stuhl², Jennifer L. Gillett-Kaufman¹

¹Entomology and Nematology Department, University of Florida, ²Center for Medical, Agricultural and Veterinary Entomology, Agricultural Research Service U.S. Department of Agriculture

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta

Joanna Kud¹, Wenjie Wang², Yulin Yuan², Allan Caplan², Joseph C. Kuhl², Louise-Marie Dandurand¹, Fangming Xiao²

¹Department of Entomology, Plant Pathology and Nematology, University of Idaho, ²Department of Plant Sciences, University of Idaho

Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors

Keni Vidilaseris*¹, Niklas G. Johansson*², Ainoleena Turku², Alexandros Kiriazis², Gustav Boije af Gennäs², Jari Yli-Kauhaluoma², Henri Xhaard², Adrian Goldman^1,3

¹Research Program in Molecular and Integrative Biosciences, University of Helsinki, ²Drug Research Program, Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, ³School of Biomedical Sciences and Astbury Centre for Structural Molecular Biology, University of Leeds

Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling

Steven E. Guard¹, Christopher C. Ebmeier¹, William M. Old^1,2

¹Department of Molecular, Cellular and Developmental Biology, University of Colorado, ²Linda Crnic Institute for Down Syndrome, University of Colorado School of Medicine

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

David André Coucheron¹, Øystein Ivar Helle¹, Cristina Ionica Øie², Jean-Claude Tinguely¹, Balpreet Singh Ahluwalia¹

¹Department of Physics and Technology, UiT The Arctic University of Norway, ²Vascular Biology Research Group, Department of Medical Biology, UiT The Arctic University of Norway

Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase

Ryan K. Messer¹, Miguel A. Lopez Jr.¹, Gayan Senavirathne¹, Kristine E. Yoder¹

¹Cancer Biology and Genetics, College of Medicine, The Ohio State University

Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations

Mirna Swayden¹, Aurélie Dobric¹, Yolande Berthois¹, Hugo Villalba¹, Stéphane Audebert¹, Luc Camoin¹, Juan Iovanna¹, Philippe Soubeyran¹

¹Aix-Marseille Univ, INSERM, CNRS, Institut Paoli-Calmettes, CRCM

PeptiQuick, a One-Step Incorporation of Membrane Proteins into Biotinylated Peptidiscs for Streamlined Protein Binding Assays

James W. Saville¹, Lucy Troman², Franck Duong Van Hoa¹

¹Department of Biochemistry, University of British Columbia, ²School of Biochemistry, University of Bristol

SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer

Fernando Lopitz-Otsoa¹, Teresa C Delgado¹, Sofía Lachiondo-Ortega¹, Mikel Azkargorta², Felix Elortza², Manuel S Rodríguez³, María Luz Martínez-Chantar¹

¹Liver Disease and Liver Metabolism Lab, CIC bioGUNE, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), ²Proteomics Platforms, CIC bioGUNE, ProteoRED-ISCIII, CIBERehd, ³Advanced Technology Institute in Life Sciences (ITAV)-CNRS-IPBS, UbiCARE

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Alien Balian*^1,2,3, Javier Garcia Gonzalez*^1,2,3, Nora Bastida^1,3, Khadija-Tul Kubra Akhtar^1,3, Baris A. Borsa^1,2,3, Frank J. Hernandez^1,2,3

¹Department of Physics, Chemistry and Biology, Linköping University, ²Wallenberg Centre for Molecular Medicine (WCMM), ³Nucleic Acids Technologies Laboratory (NAT-lab), Linköping University

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry

Michael Vilkhovoy¹, David Dai¹, Sandra Vadhin*¹, Abhinav Adhikari*¹, Jeffrey D. Varner¹

¹Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays

Fernanda Festa^1,2, Joshua Labaer¹

¹Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, ²Department of Pediatrics, and Biochemistry/Molecular Biology, College of Medicine, Pennsylvania State University

Methods Collection

In Vivo Calcium Imaging in C. elegans Body Wall Muscles

Ashley A. Martin^1,2, Simon Alford³, Janet E. Richmond¹

¹Department of Biological Sciences, University of Illinois at Chicago, ²Department of Integrative Biology and Physiology, University of Minnesota, ³Department of Anatomy and Cell Biology, University of Illinois at Chicago

Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation

Jingjie Hu¹, Nathan D. Derr^1,2

¹Department of Biological Sciences, Smith College, ²Center for Microscopy and Imaging, Smith College

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

Catrin Swantje Müller¹, Wolfgang Bildl¹, Norbert Klugbauer², Alexander Haupt¹, Bernd Fakler^1,3, Uwe Schulte^1,3,4

¹Institute of Physiology, Faculty of Medicine, University of Freiburg, Freiburg, Germany, ²Institute of Experimental and Clinical Pharmacology and Toxicology, Faculty of Medicine, University of Freiburg, Freiburg, Germany, ³Signalling Research Centres BIOSS and CIBSS, Freiburg, Germany, ⁴Logopharm GmbH, Germany

Examining the Dynamics of Cellular Adhesion and Spreading of Epithelial Cells on Fibronectin During Oxidative Stress

Caitlin E Tolbert¹, Lindsey Palmquist², Hannah Lee Dixon², Melissa C. Srougi^2,3

¹Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill, ²Department of Chemistry, High Point University, ³Biotechnology Program and the Department of Molecular Biomedical Sciences, North Carolina State University

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling

Stefan Ilic¹, Shira Cohen¹, Ariel Afek², Raluca Gordan², David B. Lukatsky¹, Barak Akabayov¹

¹Department of Chemistry, Ben-Gurion University of the Negev, ²Center for Genomic and Computational Biology, Department of Biostatistics and Bioinformatics, Duke University

Perturbing Endothelial Biomechanics via Connexin 43 Structural Disruption

Md. Mydul Islam¹, Robert L. Steward, Jr.^1,2

¹Department of Mechanical and Aerospace Engineering, University of Central Florida, ²Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Katherine M. Murphy¹, Siwon Chung¹, Shruti Fogla¹, Hana B. Minsky¹, Karen Yong Zhu¹, Philipp Zerbe¹

¹Department of Plant Biology, University of California Davis

Interactions with and Membrane Permeabilization of Brain Mitochondria by Amyloid Fibrils

Ramin Zadali¹, Ebrahim Rostampour Ghareghozloo*¹, Mohammad Ramezani*¹, Vahid Hassani*¹, Yasin Rafiei*¹, Shiva Mohammadi Chiyaneh¹, Ali Akbar Meratan¹

¹Department of Biological Sciences, Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan, 45137-66731, Iran

Quantification of Coenzyme A in Cells and Tissues

Matthew W. Frank¹, Chitra Subramanian¹, Charles O. Rock¹, Suzanne Jackowski¹

¹Department of Infectious Diseases, St. Jude Children's Research Hospital

A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput

Nasir Malik¹, Rohini Manickam¹, Muznabanu Bachani¹, Joseph P. Steiner¹

¹National Institute of Neurological Disorders and Stroke (NINDS), Neurotherapeutic Development Unit (NTDU), National Institutes of Health (NIH)

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