JoVE Journal
Biochemistry
Analysis of AtHIRD11 Intrinsic Disorder and Binding Towards Metal Ions by Capillary Gel Electrophoresis and Affinity Capillary Electrophoresis
1Institute of Medicinal and Pharmaceutical Chemistry, TU Braunschweig
Biochemistry
A Western Blotting Protocol for Small Numbers of Hematopoietic Stem Cells
1Division of Experimental Hematology, Cincinnati Children's Hospital Medical Center, 2Department of Cell and Developmental Biology, Abramson Family Cancer Research Institute, Perelman School of Medicine, University of Pennsylvania, 3Department of Obstetrics and Gynecology, Southwest Hospital, Third Military Medical University
Biochemistry
High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis (QDB)
1Medicine and Pharmacy Research Center, Binzhou Medical University, 2Yantai Zestern Biotechnique Co. LTD, 3Precision Medicine research center, Binzhou Medical University, 4Department of Chemistry - BMC, Uppsala University
Biochemistry
Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells
Biochemistry
Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
1Center for Applied Tissue Engineering and Regenerative Medicine, Munich University of Applied Sciences, 2FG Protein Biochemistry & Cellular Microbiology, Munich University of Applied Sciences, 3Center for Nano Science, Ludwig-Maximilians-Universität München, 4Klinik für Unfallchirurgie, Orthopädie und Plastische Chirurgie, University Medical Center Göttingen
Biochemistry
An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
Biochemistry
Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
Biochemistry
Sampling and Pretreatment of Tooth Enamel Carbonate for Stable Carbon and Oxygen Isotope Analysis
1Max Planck Institute for the Science of Human History, 2School of Archaeology, University of Oxford
Biochemistry
Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering
1Xtal Concepts GmbH, 2Institute for Biochemistry and Molecular Biology, Laboratory for Structural Biology of Infection and Inflammation c/o DESY, University of Hamburg, 3The Hamburg Center for Ultrafast Imaging, University of Hamburg
Biochemistry
Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
1Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 2Department of Chemistry, Massachusetts Institute of Technology
Biochemistry
2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
1Max Planck Institute of Molecular Plant Physiology
Biochemistry
Expression and Purification of Mammalian Bestrophin Ion Channels
1Department of Pharmacology and Physiology, University of Rochester, School of Medicine and Dentistry
Biochemistry
A Direct Force Probe for Measuring Mechanical Integration Between the Nucleus and the Cytoskeleton
1Department of Chemical Engineering, University of Florida
Biochemistry
In Vitro Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers
1Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry
Biochemistry
Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software
1Cellular Imaging Group, Wellcome Centre Human Genetics, University of Oxford, 2Division of Structural Biology, Wellcome Centre Human Genetics, University of Oxford, 3Dynamic Structural Virology Group, Biocruces Health Research Centre, 4IKERBASQUE, Basque Foundation for Science
Biochemistry
The Identification of Sea Lamprey Pheromones Using Bioassay-Guided Fractionation
1Department of Fisheries and Wildlife, Michigan State University
Biochemistry
Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques
1Program in Molecular Medicine, The Hospital for Sick Children Research Institute, 2Department of Biochemistry, University of Toronto, 3Department of Immunology, University of Toronto
Biochemistry
Assessing the Viability of a Synthetic Bacterial Consortium on the In Vitro Gut Host-microbe Interface
1Center for Microbial Ecology and Technology (CMET), Ghent University
Biochemistry
Purification and Reconstitution of TRPV1 for Spectroscopic Analysis
1Department of Physiology, University of Tennessee Health Science Center, 2Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, 3CuriRX, Inc.
Biochemistry
In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria
1Institute of Biochemistry, Graz University of Technology
Biochemistry
Generation of Native, Untagged Huntingtin Exon1 Monomer and Fibrils Using a SUMO Fusion Strategy
Biochemistry
Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+
1Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, 2College of Life Science, Nanjing Agricultural University
Biochemistry
