JoVE Journal
Biochemistry
Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification
1Department of Biology, Queen's University, 2Department of Biomedical and Molecular Sciences, Queen's University, 3School of Environmental Sciences, Queen's University
Biochemistry
Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
1Department of Chemistry, University of Pittsburgh, 2SGS North America Inc., 3Large Molecule Analytical Development, Pharmaceutical Development & Manufacturing Science, Janssen Research and Development
Biochemistry
Modification and Functionalization of the Guanidine Group by Tailor-made Precursors
1Department of Chemistry, Technische Universität München
Biochemistry
Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry
1King's British Heart Foundation Centre, King's College London
Biochemistry
Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
1Department of Biotherapeutics Discovery, Immune Modulation and Biotherapeutics Discovery, Boehringer Ingelheim Pharmaceuticals, Inc., 2The Fu Foundation School of Engineering and Applied Science, Columbia University
Biochemistry
Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
1Heinrich Pette Institute, Leibniz Institute for Experimental Virology, 2Core Facility Mass Spectrometric Proteomics, University Medical Center Hamburg-Eppendorf
Biochemistry
Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
1Department of Chemistry, York University, 2The Centre for Research in Mass Spectrometry, York University, 3The Centre for Research on Biomolecular Interactions, York University
Biochemistry
Hydrolysis of a Ni-Schiff-Base Complex Using Conditions Suitable for Retention of Acid-labile Protecting Groups
1Department of Chemistry, Slippery Rock University
Biochemistry
Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
1Proteomic Mass Spectrometry, Wellcome Trust Sanger Institute
Biochemistry
A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus
1Department of Biology, Georgetown University, 2Division of Infectious Diseases, Weill Cornell Medical College, 3Department of Medicine, Weill Cornell Medical College
Biochemistry
Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures
1Research and Development, British American Tobacco
Biochemistry
A Tailored HPLC Purification Protocol That Yields High-purity Amyloid Beta 42 and Amyloid Beta 40 Peptides, Capable of Oligomer Formation
1Department of Chemistry, University of California, Santa Cruz
Biochemistry
Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones
1Department of Structural Biology, Leibniz Institute of Molecular Pharmacology (FMP-Berlin)
Biochemistry
Synthesis of 1,2-Azaborines and the Preparation of Their Protein Complexes with T4 Lysozyme Mutants
1Department of Chemistry, Boston College
Biochemistry
A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
1Department of Cancer Biology, Wake Forest School of Medicine, 2YX Genomics, 3Department of Biology, Ball State University, 4Department of Hematology and Oncology, Roper St. Francis Hospital
Biochemistry
Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
1Department of Biosciences and Nutrition, Karolinska Institutet, 2School of Technology and Health, KTH Royal Institute of Technology
Biochemistry
Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
1Plant Biology Section, School of Integrative Plant Science, Cornell University, 2Department of Developmental Genetics, University of Wyoming
Biochemistry
Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen
1Analytical Research & Development, Sanofi Pasteur Limited
Biochemistry
Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues
1Department of Chemistry and Biochemistry, Central Michigan University, 2Department of Chemistry, University of Southern Maine
Biochemistry
Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System
1Institute of Biophysics, Ulm University
Biochemistry
A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing
1Department of Medicine, Unit of Computational Medicine, Karolinska Institutet, 2Center for Molecular Medicine, Karolinska Institutet, 3Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry 2, Karolinska Institutet, 4Department of Medicine, University of California San Diego
Biochemistry
Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells
1Department of Microbiology, The University of Texas Southwestern Medical Center
Biochemistry
Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion
1Department of Mathematical and Physical Sciences, Faculty of Science, Japan Women's University, 2Department of Bioorganization Research, Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, 3Department of Life and Coordination-Complex Molecular Science, Institute for Molecular Science, National Institutes of Natural Sciences, 4Research Center for Complex Systems Biology, The University of Tokyo
Biochemistry
