
JoVE Journal
Biochemistry
Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods
1Department of Sciences and Technologies (DST), University of Sannio, 2Endocannabinoid Research Group, Institute of Biomolecular Chemistry, 3Department of Veterinary Medicine and Animal Productions, University of Naples Federico II
Biochemistry
Expression, Purification, Crystallization, and Enzyme Assays of Fumarylacetoacetate Hydrolase Domain-Containing Proteins
1Research Institute for Biomedical Aging Research, University of Innsbruck Austria, 2Center for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck Austria, 3Division of Genetic Epidemiology, Medical University of Innsbruck Austria, 4Faculty of Chemistry, Department of Organic Chemistry, University of Vienna Austria
Biochemistry
Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS)
1Wyatt Technology Corporation, 2Wolfson Centre for Applied Structural Biology, The Alexander Silberman Institute of Life Science, The Hebrew University of Jerusalem, 3Danyel Biotech Ltd.
Biochemistry
Nitropeptide Profiling and Identification Illustrated by Angiotensin II
1Mass Spectrometry Core Facility, School of Life Sciences, Westlake University, 2Department of Biological Sciences, University of Notre Dame, 3Department of Urology, Shanghai East Hospital, Tongji University School of Medicine, 4Boler-Parseghian Center for Rare and Neglected Diseases, University of Notre Dame, 5Harper Cancer Research Institute, University of Notre Dame, 6Tumor Microenvironment and Metastasis Program, Indiana University Melvin and Bren Simon Cancer Center
Biochemistry
Study on the Metabolism of Six Systemic Insecticides in a Newly Established Cell Suspension Culture Derived from Tea (Camellia Sinensis L.) Leaves
1State Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Science & Technology, Anhui Province Key Lab of Analysis and Detection for Food Safety, 2School of Resource & Environment, Anhui Agricultural University, Key Laboratory of Agri-food Safety of Anhui Province
Biochemistry
Real-Time, Semi-Automated Fluorescent Measurement of the Airway Surface Liquid pH of Primary Human Airway Epithelial Cells
1Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, Newcastle University, 2Respiratory Group, Institute of Cellular Medicine, Faculty of Medical Sciences, Newcastle University, 3Paediatric Respiratory Medicine, Great North Children's Hospital, Newcastle upon Tyne Hospitals NHS Foundation Trust, 4Boehringer Ingelheim Pharma GmbH & Co
Biochemistry
Looking Outwards: Isolation of Cyanobacterial Released Carbohydrate Polymers and Proteins
1Bioengineering and Synthetic Microbiology Group, Instituto de Investigação e Inovação em Saude, Universidade do Porto, 2Instituto de Biologia Celular e Molecular, Universidade do Porto, 3Instituto de Ciências Biomédicas Abel Salazar, 4Departamento de Biologia, Faculdade de Ciências, Universidade do Porto
Biochemistry
Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo
1Blood Research Institute, Blood Center of Wisconsin, 2Department of Medicine, Medical College of Wisconsin, 3Department of Surgery, The University of Alabama at Birmingham, 4Department of Biomedical Engineering, The University of Alabama at Birmingham, 5GBS Program, Graduate School, The University of Alabama at Birmingham
Biochemistry
Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells
1Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 2Department of Chemistry, Skidmore College, 3Candiac MR Center, Beth Israel Deaconess Medical Center, 4Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero
Biochemistry
Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate
1Center for Pathobiochemistry and Genetics, Institute of Medical Chemistry and Pathobiochemistry, Medical University Vienna, 2School of Medical Engineering and Applied Social Sciences, University of Applied Sciences Upper Austria
Biochemistry
Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay
1Division of Molecular Biology, Biocenter, Medical University of Innsbruck, 2Biology Department, Maynooth University, 3Maynooth University Human Health Research Institute
Biochemistry
Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
1Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, 2Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute of Molecular Functional Materials, State Key Laboratory of Neuroscience, Division of Biomedical Engineering, and Division of Life Science, Hong Kong University of Science and Technology
Biochemistry