JoVE Journal
Biochemistry
A Tailored HPLC Purification Protocol That Yields High-purity Amyloid Beta 42 and Amyloid Beta 40 Peptides, Capable of Oligomer Formation
1Department of Chemistry, University of California, Santa Cruz
Biochemistry
Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones
1Department of Structural Biology, Leibniz Institute of Molecular Pharmacology (FMP-Berlin)
Biochemistry
Synthesis of 1,2-Azaborines and the Preparation of Their Protein Complexes with T4 Lysozyme Mutants
1Department of Chemistry, Boston College
Biochemistry
A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
1Department of Cancer Biology, Wake Forest School of Medicine, 2YX Genomics, 3Department of Biology, Ball State University, 4Department of Hematology and Oncology, Roper St. Francis Hospital
Biochemistry
Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
1Department of Biosciences and Nutrition, Karolinska Institutet, 2School of Technology and Health, KTH Royal Institute of Technology
Biochemistry
Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
1Plant Biology Section, School of Integrative Plant Science, Cornell University, 2Department of Developmental Genetics, University of Wyoming
Biochemistry
Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen
1Analytical Research & Development, Sanofi Pasteur Limited
Biochemistry
Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues
1Department of Chemistry and Biochemistry, Central Michigan University, 2Department of Chemistry, University of Southern Maine
Biochemistry
Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System
1Institute of Biophysics, Ulm University
Biochemistry
A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing
1Department of Medicine, Unit of Computational Medicine, Karolinska Institutet, 2Center for Molecular Medicine, Karolinska Institutet, 3Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry 2, Karolinska Institutet, 4Department of Medicine, University of California San Diego
Biochemistry
Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells
1Department of Microbiology, The University of Texas Southwestern Medical Center
Biochemistry
Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion
1Department of Mathematical and Physical Sciences, Faculty of Science, Japan Women's University, 2Department of Bioorganization Research, Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, 3Department of Life and Coordination-Complex Molecular Science, Institute for Molecular Science, National Institutes of Natural Sciences, 4Research Center for Complex Systems Biology, The University of Tokyo
Biochemistry
Laboratory Scale Production and Purification of a Therapeutic Antibody
1Faculty of Pharmacy, University of Sydney
Biochemistry
A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis
1Graduate School of Frontier Biosciences, Osaka University, 2Department of Biotechnology and Genetic Engineering, Jahangirnagar University, 3Division of Cell Regeneration and Transplantation, School of Medicine, Nihon University, 4Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, 5Graduate School of Life and Medical Sciences, Doshisha University
Biochemistry
Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
1University Children's Hospital Basel (UKBB), University of Basel, 2Department of Clinical Research, University of Basel
Biochemistry
Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary
1Unidad de Investigación y Desarrollo, Probiomed S.A. de C.V.
Biochemistry
Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
1Department of Veterinary & Biomedical Sciences, University of Minnesota, 2Department of Veterinary Biosciences, Ohio State University, 3School of Biotechnology, Gautam Buddha University
Biochemistry
Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay
1Department of Chemistry, Stony Brook University, 2Department of Physiology and Biophysics, Stony Brook University
Biochemistry
An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
1Laboratory of Biological Diversity, Department of Evolutionary Biology and Biodiversity, National Institute for Basic Biology, 2Department of Basic Biology, SOKENDAI (The Graduate University for Advanced Studies), 3Department of Cell Biology, National Institute for Basic Biology
Biochemistry
Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
Biochemistry
A Rapid Laser Probing Method Facilitates the Non-invasive and Contact-free Determination of Leaf Thermal Properties
1Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e. V., 2Institute for Molecular Biotechnology, RWTH Aachen University, 3Fraunhofer Institute for Laser Technology ILT, Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e. V.
Biochemistry
SorLA and CLC:CLF-1-dependent Downregulation of CNTFRα as Demonstrated by Western Blotting, Inhibition of Lysosomal Enzymes, and Immunocytochemistry
1Department of Biomedicine, Aarhus University
Biochemistry
Combining Wet and Dry Lab Techniques to Guide the Crystallization of Large Coiled-coil Containing Proteins
1Department of Biological Sciences, University of Pittsburgh, 2Institute of Structural Biology, German Research Center for Environmental Health
Biochemistry
