JoVE Journal

Biochemistry

JoVE Biochemistry includes methods and experimental approaches to study the structure, function, and interactions of biological molecules.

‹1…7 8 9 10 11 12…16 › 361 VIDEO ARTICLES

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Michael J. Stephenson¹, James Reed¹, Bastiaan Brouwer¹, Anne Osbourn¹

¹John Innes Centre

JoVE 58169

Sampling and Pretreatment of Tooth Enamel Carbonate for Stable Carbon and Oxygen Isotope Analysis

Alicia Ventresca Miller¹, Ricardo Fernandes^1,2, Anneke Janzen¹, Ayushi Nayak¹, Jillian Swift¹, Jana Zech¹, Nicole Boivin¹, Patrick Roberts¹

¹Max Planck Institute for the Science of Human History, ²School of Archaeology, University of Oxford

JoVE 58002

Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering

Daniela Baitan^1,2, Robin Schubert^2,3, Arne Meyer¹, Karsten Dierks¹, Markus Perbandt^2,3, Christian Betzel^2,3

¹Xtal Concepts GmbH, ²Institute for Biochemistry and Molecular Biology, Laboratory for Structural Biology of Infection and Inflammation c/o DESY, University of Hamburg, ³The Hamburg Center for Ultrafast Imaging, University of Hamburg

JoVE 57070

Detection and Isolation of Apoptotic Bodies to High Purity

Thanh Kha Phan¹, Ivan KH Poon¹, Georgia K Atkin-Smith¹

¹Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University

JoVE 58317

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Alexandra J. Machen¹, Pierce T. O'Neil¹, Bradley L. Pentelute², Maria T. Villar¹, Antonio Artigues¹, Mark T. Fisher¹

¹Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, ²Department of Chemistry, Massachusetts Institute of Technology

JoVE 57902

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes

Marcin Luzarowski*¹, Izabela Wojciechowska*¹, Aleksandra Skirycz¹

¹Max Planck Institute of Molecular Plant Physiology

JoVE 57720

Methods Collection

Expression and Purification of Mammalian Bestrophin Ion Channels

Alec Kittredge¹, Nancy Ward¹, Austin Hopiavuori¹, Yu Zhang ¹, Tingting Yang¹

¹Department of Pharmacology and Physiology, University of Rochester, School of Medicine and Dentistry

JoVE 57832

A Direct Force Probe for Measuring Mechanical Integration Between the Nucleus and the Cytoskeleton

Qiao Zhang¹, Andrew C. Tamashunas¹, Tanmay P. Lele¹

¹Department of Chemical Engineering, University of Florida

JoVE 58038

In Vitro Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers

Beatrice Ramm*¹, Philipp Glock*¹, Petra Schwille¹

¹Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry

JoVE 58139

Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software

Rory Nolan¹, Luis A. Alvarez¹, Samuel C. Griffiths², Jonathan Elegheert², Christian Siebold², Sergi Padilla-Parra^1,2,3,4

¹Cellular Imaging Group, Wellcome Centre Human Genetics, University of Oxford, ²Division of Structural Biology, Wellcome Centre Human Genetics, University of Oxford, ³Dynamic Structural Virology Group, Biocruces Health Research Centre, ⁴IKERBASQUE, Basque Foundation for Science

JoVE 58157

Methods Collection

The Identification of Sea Lamprey Pheromones Using Bioassay-Guided Fractionation

Anne M. Scott¹, Ke Li¹, Weiming Li¹

¹Department of Fisheries and Wildlife, Michigan State University

JoVE 58059

Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques

June Ereño-Orbea*¹, Taylor Sicard*^1,2, Hong Cui¹, Indira Akula¹, Jean-Philippe Julien^1,2,3

¹Program in Molecular Medicine, The Hospital for Sick Children Research Institute, ²Department of Biochemistry, University of Toronto, ³Department of Immunology, University of Toronto

JoVE 57750

Assessing the Viability of a Synthetic Bacterial Consortium on the In Vitro Gut Host-microbe Interface

Marta Calatayud Arroyo¹, Tom Van de Wiele¹, Emma Hernandez-Sanabria¹

¹Center for Microbial Ecology and Technology (CMET), Ghent University

JoVE 57699

Purification and Reconstitution of TRPV1 for Spectroscopic Analysis

Francisco J. Sierra-Valdez¹, Richard A. Stein², Phanindra Velissety^1,3, Valeria Vasquez¹, Julio F. Cordero-Morales¹

¹Department of Physiology, University of Tennessee Health Science Center, ²Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, ³CuriRX, Inc.

JoVE 57796

In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria

Eveline Brodl¹, Johannes Niederhauser¹, Peter Macheroux¹

¹Institute of Biochemistry, Graz University of Technology

JoVE 57881

Generation of Native, Untagged Huntingtin Exon1 Monomer and Fibrils Using a SUMO Fusion Strategy

Andreas Reif¹, Anass Chiki¹, Jonathan Ricci¹, Hilal A. Lashuel¹

¹Laboratory of Molecular and Chemical Biology of Neurodegeneration, Ecole Polytechnique Fédérale de Lausanne (EPFL)

JoVE 57506

Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H⁺

Ya-Min Du¹, Shen-Li Zheng¹, Li Liu¹, Josef Voglmeir¹, Gabriel Yedid²

¹Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, ²College of Life Science, Nanjing Agricultural University

JoVE 57979

Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast

Anita Carija¹, Salvador Ventura¹, Susanna Navarro¹

¹Institut de Biotecnologia i Biomedicina and Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona

JoVE 57470

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues

Dana M. Freund¹, Katherine A. Sammons², Nokwanda P. Makunga³, Jerry D. Cohen¹, Adrian D. Hegeman²

¹Department of Horticultural Science, Microbial and Plant Genomics Institute, University of Minnesota, ²Department of Horticultural Science, Department of Plant and Microbial Biology, Microbial and Plant Genomics Institute, University of Minnesota, ³Department of Botany and Zoology, Stellenbosch University

JoVE 57949

Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue

David Jelinek^1,2, Aimee Flores^1,3, Melanie Uebelhoer¹, Vincent Pasque², Kathrin Plath^2,3, M. Luisa Iruela-Arispe^1,3, Heather R. Christofk^2,3, William E. Lowry^1,3, Hilary A. Coller^1,2,3

¹Department of Molecular, Cell and Developmental Biology, UCLA, ²Department of Biological Chemistry, David Geffen School of Medicine, ³Molecular Biology Institute Interdepartmental Program, UCLA

JoVE 57760

Fluorescence Live-cell Imaging of the Complete Vegetative Cell Cycle of the Slow-growing Social Bacterium Myxococcus xanthus

Dominik Schumacher¹, Lotte Søgaard-Andersen¹

¹Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology

JoVE 57860

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Kang-Yi Su*^1,2, Steven D. Goodman*³, Hung-Ming Lai¹, Rong-Syuan Yen¹, Wei-Yao Hu¹, Wern-Cherng Cheng², Liang-In Lin^1,2, Ya-Chien Yang^1,2, Woei-Horng Fang^1,2

¹Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, ²Department of Laboratory Medicine, National Taiwan University Hospital, ³Center for Microbial Pathogenesis, Nationwide Children's Hospital and the Department of Pediatrics, The Ohio State University

JoVE 57862

Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry

Rosi Fassler*¹, Nufar Edinger*¹, Oded Rimon¹, Dana Reichmann¹

¹Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, Safra Campus Givat Ram, The Hebrew University of Jerusalem

JoVE 57806

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

Tabitha A. Peterson¹, Mark A. Stamnes¹, Robert C. Piper¹

¹Molecular Physiology and Biophysics, University of Iowa

JoVE 57801

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