JoVE Journal

Biochemistry

JoVE Biochemistry includes methods and experimental approaches to study the structure, function, and interactions of biological molecules.

‹1…7 8 9 10 11 12…18 › 420 VIDEO ARTICLES

Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins

Eva Königshausen¹, Sebastian A. Potthoff¹, Raphael Haase¹, Catherine Meyer-Schwesinger², Ernest Kaufmann¹, L. Christian Rump¹, Johannes Stegbauer¹, Lorenz Sellin¹, Ivo Quack¹, Magdalena Woznowski¹

¹Department of Nephrology, Medical Faculty, Heinrich-Heine-University, ²Institute of Cellular and Integrative Physiology, University Medical Center Hamburg-Eppendorf

JoVE 58542

Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation

Beáta Bozóki*^1,2, János András Mótyán*¹, Márió Miczi¹, Lívia Diána Gazda¹, József Tőzsér¹

¹Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, ²Biotechnology Research Department, Gedeon Richter Plc

JoVE 58824

Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K

Hanan B. Elajaili¹, Laura Hernandez-Lagunas¹, Kalina Ranguelova², Sergey Dikalov³, Eva Nozik-Grayck¹

¹Cardiovascular Pulmonary Research Laboratories and Pediatric Critical Care Medicine, Department of Pediatrics, University of Colorado Anschutz Medical Campus, ²Bruker BioSpin Corp, ³Department of Medicine, Division of Clinical Pharmacology, Vanderbilt University Medical Center

JoVE 58461

A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation

Javier A. Jaimes*^1,2, Jean K. Millet*^1,3, Monty E. Goldstein^1,4, Gary R. Whittaker¹, Marco R. Straus¹

¹Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, ²Department of Microbiology, College of Agricultural and Life Sciences, Cornell University, ³Virologie et Immunologie Moléculaires, Domaine de Vilvert, INRA, ⁴Department of Cell Biology and Molecular Genetics, University of Maryland

JoVE 58892

Identification of Functional Protein Regions Through Chimeric Protein Construction

Juan M. Adrian-Segarra¹, Holger Lörchner^1,2, Thomas Braun^1,2, Jochen Pöling^1,2

¹Department of Cardiac Development and Remodelling, Max Planck Institute for Heart and Lung Research, ²Partner site Rhein-Main, German Centre for Cardiovascular Research (DZHK)

JoVE 58786

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Bushra Husain¹, Sairupa Paduchuri¹, Sree R. Ramani², Nadia Martinez-Martin¹

¹Receptor Discovery group, Microchemistry, Proteomics and Lipidomics Department, Genentech, ²Portfolio Management and Operations, Genentech

JoVE 58451

Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment

Magali Court^1,2, Marie Malier^1,2, Arnaud Millet^1,2,3

¹Team Mechanobiology, Immunity and Cancer, Institute for Advanced Biosciences, INSERM U1209, CNRS UMR5309, ²Université Grenoble Alpes, ³Pôle Recherche, Centre Hospitalier Universitaire des Alpes

JoVE 58727

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

Andreas Weber¹, Julia Christin Wehmeyer¹, Vera Schmidt¹, Artur Lichtenberg¹, Payam Akhyari¹

¹Department of Cardiovascular Surgery, Medical Faculty, Heinrich-Heine-University

JoVE 58731

Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow

Jae Gon Kim*¹, Sang Woong Park*², Kyung Chul Shin¹, Bokyung Kim¹, Doyoung Byun³, Young Min Bae¹

¹Department of Physiology, KU Open Innovation Center, Research Institute of Medical Science, Konkuk University School of Medicine, ²Department of Emergency Medical Services, Eulji University, ³Department of Mechanical Engineering, Sungkyunkwan University

JoVE 58228

Extraction and Analysis of Taiwanese Green Propolis

Chun-Ting Chen¹, Yi-Hsuan Chien¹, Yu-Hsiang Yu¹, Yue-Wen Chen¹

¹Department of Biotechnology and Animal Science, National Ilan University

JoVE 58743

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Emery Haley*¹, Wooyoung Choi*¹, Chen Fan¹, Weinan Sun^2,3, Juan Du¹, Wei Lü¹

¹Van Andel Institute, ²Vollum Institute, ³Janelia Research Campus

JoVE 58754

How to Quantify the Fraction of Photoactivated Fluorescent Proteins in Bulk and in Live Cells

Vanessa Chen¹, Malte Renz¹

¹Gynecologic Oncology Division, Stanford University School of Medicine

JoVE 58588

Measuring and Interpreting Oxygen Consumption Rates in Whole Fly Head Segments

Louisa Jutta Dietz¹, Anuroop Venkateswaran Venkatasubramani¹, Annika Müller-Eigner², Martin Hrabe de Angelis^3,4,5, Axel Imhof¹, Lore Becker³, Shahaf Peleg^2,6,7

¹Munich Center of Integrated Protein Science and Biomedical Center, Ludwig-Maximilians University of Munich, ²Laboratory for Metabolism and Epigenetics in Aging, Leibniz Institute for Farm Animal Biology (FBN), ³German Mouse Clinic, Helmholtz Zentrum Munich, German Research Center for Environment and Health (GmbH), ⁴German Center for Diabetes Research (DZD), ⁵Chair of Experimental Genetics, School of Life Science Weihenstephan, Technische Universität München, ⁶Laboratory for Metabolism and Epigenetics in Brain Aging, Institute of Neuroregeneration & Neurorehabilitation of Qingdao University, ⁷Molecular Biology Division, Biomedical Center, Faculty of Medicine, Ludwig-Maximilians University of Munich

JoVE 58601

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker

Aleksandra Skrajna¹, Xiao-Cui Yang¹, Zbigniew Dominski^1,2

¹Integrative Program for Biological and Genome Sciences, University of North Carolina at Chapel Hill, ²Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill

JoVE 58697

Studying Protein Import into Chloroplasts Using Protoplasts

Junho Lee¹, Hyangju Kang¹, Inhwan Hwang^1,2

¹Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, ²Department of Life Sciences, Pohang University of Science and Technology

JoVE 58441

Methods Collection

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

Valentin Dunsing¹, Salvatore Chiantia¹

¹Institute for Biochemistry and Biology, Cell Membrane Biophysics Group, University of Potsdam

JoVE 58582

Crystal Structure of the N-terminal Domain of Ryanodine Receptor from Plutella xylostella

Bidhan Chandra Nayak*¹, Jie Wang*¹, Lianyun Lin*^1,2,3,4, Weiyi He^2,3,4, Minsheng You^2,3,4, Zhiguang Yuchi^1,2

¹Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, Collaborative Innovation Center of Chemical Science and Engineering, School of Pharmaceutical Science and Technology, Tianjin University, ²State Key Laboratory of Ecological Pest Control for Fujian/Taiwan Crops and Institute of Applied Ecology, Fujian Agriculture and Forestry University, ³Joint International Research Laboratory of Ecological Pest Control, Ministry of Education, Fuzhou, ⁴Fujian-Taiwan Joint Centre for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University

JoVE 58568

Analysis of β-Amyloid-induced Abnormalities on Fibrin Clot Structure by Spectroscopy and Scanning Electron Microscopy

Pradeep K. Singh*¹, Hanna E. Berk-Rauch*¹, Nadine Soplop², Kunihiro Uryu², Sidney Strickland¹, Hyung Jin Ahn¹

¹Patricia and John Rosenwald Laboratory of Neurobiology and Genetics, Rockefeller University, ²Electron Microscopy Resource Center, Rockefeller University

JoVE 58475

Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli

Teresa Cordero¹, Verónica Aragonés¹, José-Antonio Daròs¹

¹Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas-Universitat Politècnica de València, Spain

JoVE 58472

Measuring Liver Mitochondrial Oxygen Consumption and Proton Leak Kinetics to Estimate Mitochondrial Respiration in Holstein Dairy Cattle

Heidi A. Rossow¹, Gabriela Acetoze², John Champagne¹, Jon J. Ramsey³

¹Veterinary Medicine Teaching and Research Center, University of California Davis, ²Archer Daniels Midland, ³Department of Molecular Biosciences, University of California Davis

JoVE 58387

Using High Content Imaging to Quantify Target Engagement in Adherent Cells

Hanna Axelsson^1,2, Helena Almqvist^1,2, Brinton Seashore-Ludlow^1,3

¹Chemical Biology Consortium Sweden, Science for Life Laboratory, Karolinska Institutet, Solna, ²Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Solna, ³Science for Life Laboratory, Department of Oncology-Pathology, Karolinska Institutet, Stockholm

JoVE 58670

Methods Collection

Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay

Javad Alizadeh^1,2, Shahla Shojaei^1,2,3, Simone da Silva Rosa¹, Adel Rezaei Moghadam^1,2, Amir A. Zeki⁴, Mohammad Hashemi⁵, Marek J. Los^6,7,8, Joseph W. Gordon^1,9, Saeid Ghavami^1,2,10

¹Department of Human Anatomy and Cell Science, Rady Faculty of Health Sciences, Max Rady College of Medicine, University of Manitoba, ²Biology of Breathing Theme, Children Hospital Research Institute of Manitoba, University of Manitoba, ³Department of Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, ⁴Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Internal Medicine, Center for Comparative Respiratory Biology and Medicine, ⁵Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, ⁶Department of Molecular Biology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, ⁷Centre de biophysique moléculaire - UPR 4301, Centre national de la recherche scientifique (CNRS) CS80054, ⁸LinkoCare Life Sciences AB, ⁹College of Nursing and Children's Hospital Research Institute of Manitoba, Rady Faculty of Health Sciences, University of Manitoba, ¹⁰Health Policy Research Center, Institute of Health, Shiraz University of Medical Sciences

JoVE 57646

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Tyler Mrozowich¹, Steffane McLennan², Michael Overduin^2,4, Trushar R. Patel^1,3,4

¹Alberta RNA Research and Training Institute, Department of Chemistry and Biochemistry, University of Lethbridge, ²Department of Biochemistry, University of Alberta, ³Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine, University of Calgary, ⁴DiscoveryLab, Faculty of Medicine & Dentistry, University of Alberta

JoVE 58538

Methods Collection

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST)

Graeden Winkelaar¹, Catharine Trieber¹, Jitendra Kumar¹, Michael Overduin¹

¹Department of Biochemistry, University of Alberta

JoVE 58537

Methods Collection

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