JoVE Journal

Biochemistry

JoVE Biochemistry includes methods and experimental approaches to study the structure, function, and interactions of biological molecules.

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Oligopeptide Competition Assay for Phosphorylation Site Determination

Min Sung Joo¹, Ja Hyun Koo¹, Sol-Bi Shin², Hyungshin Yim², Sang Geon Kim¹

¹College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, ²College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Rachel A. Battaglia¹, Parijat Kabiraj¹, Helen H. Willcockson¹, Melinda Lian¹, Natasha T. Snider¹

¹Department of Cell Biology and Physiology, School of Medicine, University of North Carolina at Chapel Hill

Measuring Protein Binding to F-actin by Co-sedimentation

Jonathon A. Heier¹, Daniel J. Dickinson², Adam V. Kwiatkowski¹

¹Department of Cell Biology, University of Pittsburgh School of Medicine, ²Department of Biology, University of North Carolina at Chapel Hill

Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG

Meong Il Kim¹, Choongdeok Lee¹, Minsun Hong¹

¹Division of Biological Science and Technology, Yonsei University

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE

Toshio Moriya¹, Michael Saur¹, Markus Stabrin¹, Felipe Merino¹, Horatiu Voicu², Zhong Huang², Pawel A. Penczek², Stefan Raunser¹, Christos Gatsogiannis¹

¹Department of Structural Biochemistry, Max Planck Institute of Molecular Physiology, ²Department of Biochemistry and Molecular Biology, The University of Texas Medical School at Houston

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

Junyan Ma¹, Inna S. Yanez-Orozco², Soheila Rezaei Adariani², Drew Dolino³, Vasanthi Jayaraman³, Hugo Sanabria²

¹Department of Chemistry, Clemson University, ²Department of Physics and Astronomy, Clemson University, ³Department of Biochemistry and Molecular Biology, Center for Membrane Biology, Graduate School for Biomedical Sciences, University of Texas Health Science Center

Expression, Purification, and Antimicrobial Activity of S100A12

Emmanuel Jackson¹, Saffron Little¹, Dana S. Franklin¹, Jennifer A. Gaddy^2,3, Steven M. Damo^1,4

¹Department of Life and Physical Sciences, Fisk University, ²Tennessee Valley Healthcare Systems, U. S. Dept. of Veterans Affairs, ³Department of Medicine - Division of Infectious Diseases, Vanderbilt University Medical School, ⁴Department of Biochemistry, Vanderbilt University

Brain Membrane Fractionation: An Ex Vivo Approach to Assess Subsynaptic Protein Localization

Xavier Morató*^1,2, Marc López-Cano*^1,2, Paula M. Canas³, Rodrigo A. Cunha³, Francisco Ciruela^1,2

¹Unitat de Farmacologia, Departament Patologia i Terapèutica Experimental, Facultat de Medicina, IDIBELL, Universitat de Barcelona, L'Hospitalet de Llobregat, ²Institut de Neurociències, Universitat de Barcelona, ³Center for Neurosciences of Coimbra, Institute of Biochemistry, Faculty of Medicine, University of Coimbra

Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples

Tyler Rhinesmith¹, Bryan A. Killinger², Akhil Sharma³, Anna Moszczynska³

¹Physiology, Michigan State University, ²Center for Neurodegenerative Science Van Andel Institute, ³Pharmaceutical Sciences, Wayne State University

Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification

Melissa Bredow¹, Heather E. Tomalty¹, Virginia K. Walker^1,2,3

¹Department of Biology, Queen's University, ²Department of Biomedical and Molecular Sciences, Queen's University, ³School of Environmental Sciences, Queen's University

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)

Christina D. King¹, Joseph D. Dudenhoeffer¹, Liqing Gu², Adam R. Evans³, Renã A. S. Robinson¹

¹Department of Chemistry, University of Pittsburgh, ²SGS North America Inc., ³Large Molecule Analytical Development, Pharmaceutical Development & Manufacturing Science, Janssen Research and Development

Modification and Functionalization of the Guanidine Group by Tailor-made Precursors

Tobias G. Kapp*¹, Maximilian Fottner*¹, Horst Kessler¹

¹Department of Chemistry, Technische Universität München

Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

Javier Barallobre-Barreiro¹, Ferheen Baig¹, Marika Fava¹, Xiaoke Yin¹, Manuel Mayr¹

¹King's British Heart Foundation Centre, King's College London

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Danlin Yang¹, Ajit Singh², Helen Wu¹, Rachel Kroe-Barrett¹

¹Department of Biotherapeutics Discovery, Immune Modulation and Biotherapeutics Discovery, Boehringer Ingelheim Pharmaceuticals, Inc., ²The Fu Foundation School of Engineering and Applied Science, Columbia University

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Kathrin Rösch¹, Marcel Kwiatkowski², Hartmut Schlüter², Eva Herker¹

¹Heinrich Pette Institute, Leibniz Institute for Experimental Virology, ²Core Facility Mass Spectrometric Proteomics, University Medical Center Hamburg-Eppendorf

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

Cristina Lento¹, Shaolong Zhu¹, Kerene A. Brown¹, Ruth Knox¹, Peter Liuni¹, Derek J. Wilson^1,2,3

¹Department of Chemistry, York University, ²The Centre for Research in Mass Spectrometry, York University, ³The Centre for Research on Biomolecular Interactions, York University

LERLIC-MS/MS for In-depth Characterization and Quantification of Glutamine and Asparagine Deamidation in Shotgun Proteomics

Xavier Gallart-Palau*¹, Aida Serra*¹, Siu Kwan Sze¹

¹Division of Structural Biology and Biochemistry, School of Biological Sciences, Nanyang Technological University

Hydrolysis of a Ni-Schiff-Base Complex Using Conditions Suitable for Retention of Acid-labile Protecting Groups

Cory A. Bontrager¹, Tanner J. Geibel¹, George A. Lengyel¹

¹Department of Chemistry, Slippery Rock University

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Mercedes Pardo¹, Daniel Bode¹, Lu Yu¹, Jyoti S. Choudhary¹

¹Proteomic Mass Spectrometry, Wellcome Trust Sanger Institute

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

David J. Samuels¹, Zhe Wang², Kyu Y. Rhee^2,3, Shaun R. Brinsmade¹

¹Department of Biology, Georgetown University, ²Division of Infectious Diseases, Weill Cornell Medical College, ³Department of Medicine, Weill Cornell Medical College

Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures

Andrew Baxter¹, Emmanuel Minet¹

¹Research and Development, British American Tobacco

A Tailored HPLC Purification Protocol That Yields High-purity Amyloid Beta 42 and Amyloid Beta 40 Peptides, Capable of Oligomer Formation

Christopher J. A. Warner¹, Subrata Dutta¹, Alejandro R. Foley¹, Jevgenij A. Raskatov¹

¹Department of Chemistry, University of California, Santa Cruz

Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones

Stamatios Liokatis¹

¹Department of Structural Biology, Leibniz Institute of Molecular Pharmacology (FMP-Berlin)

Synthesis of 1,2-Azaborines and the Preparation of Their Protein Complexes with T4 Lysozyme Mutants

Hyelee Lee¹, Shih-Yuan Liu¹

¹Department of Chemistry, Boston College

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