JoVE Journal

Biology

JoVE Biology includes techniques and experimental approaches in the fields of cellular, molecular, and organismal biology.

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Glomerular Outgrowth as an Ex Vivo Assay to Analyze Pathways Involved in Parietal Epithelial Cell Activation

Jennifer Eymael¹, Laura Miesen¹, Fieke Mooren¹, Jitske Jansen^1,2, Jack Wetzels³, Johan van der Vlag⁴, Bart Smeets¹

¹Radboud Institute for Molecular Life Sciences, Department of Pathology, Radboud University Medical Center, ²Radboud Institute for Molecular Life Sciences, Amalia Children's Hospital, Department of Paediatric Nephrology, Radboud University Medical Center, ³Radboud Institute for Health Sciences, Department of Nephrology, Radboud University Medical Center, ⁴Radboud Institute for Molecular Life Sciences, Department of Nephrology, Radboud University Medical Center

JoVE 60324

High-throughput and Deep-proteome Profiling by 16-plex Tandem Mass Tag Labeling Coupled with Two-dimensional Chromatography and Mass Spectrometry

Zhen Wang*¹, Kanisha Kavdia*², Kaushik Kumar Dey¹, Vishwajeeth Reddy Pagala², Kiran Kodali², Danting Liu¹, Dong Geun Lee¹, Huan Sun¹, Surendhar Reddy Chepyala¹, Ji-Hoon Cho², Mingming Niu¹, Anthony A. High², Junmin Peng^1,2

¹Departments of Structural Biology and Developmental Neurobiology, St. Jude Children's Research Hospital, ²Center for Proteomics and Metabolomics, St. Jude Children's Research Hospital

JoVE 61684

Methods Collection

Modeling Age-Associated Neurodegenerative Diseases in Caenorhabditis elegans

Konstantinos Palikaras^1,2, Nektarios Tavernarakis^1,2

¹Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Greece, ²Department of Basic Sciences, Faculty of Medicine, University of Crete, Heraklion, 70013, Crete, Greece

JoVE 61169

Methods Collection

A Semiautomated ChIP-Seq Procedure for Large-scale Epigenetic Studies

Justin Cayford¹, Sara Herrera-da la Mata¹, Benjamin Joachim Schmiedel¹, Vivek Chandra¹, Pandurangan Vijayanad¹, Grégory Seumois¹

¹Division of Vaccine Discovery, La Jolla Institute for Immunology

JoVE 61617

A Murine Model of a Burn Wound Reconstructed with an Allogeneic Skin Graft

Océane Blaise*^1,2, Constance Duchesne*^1,2, Sébastien Banzet², Antoine Rousseau¹, Nadira Frescaline^1,2

¹Laboratoire de Physique des Plasmas, École Polytechnique, Sorbonne Université, CNRS, ²Institut de Recherche Biomédicale des Armées, Clamart, INSERM UMRS-MD

JoVE 61339

Quantification of Insoluble Protein Aggregation in Caenorhabditis elegans during Aging with a Novel Data-Independent Acquisition Workflow

Xueshu Xie*¹, Manish Chamoli*¹, Dipa Bhaumik¹, Renuka Sivapatham¹, Suzanne Angeli¹, Julie K. Andersen¹, Gordon J. Lithgow¹, Birgit Schilling¹

¹Buck Institute for Research on Aging

JoVE 61366

An Ex Vivo Choroid Sprouting Assay of Ocular Microvascular Angiogenesis

Yohei Tomita¹, Zhuo Shao², Bertan Cakir¹, Yumi Kotoda¹, Zhongjie Fu^1,3, Lois E.H. Smith¹

¹Department of Ophthalmology, Boston Children's Hospital, Harvard Medical School, ²Department of Clinical and Metabolic Genetics, Hospital for Sick Children, University of Toronto, ³Manton Center for Orphan Disease, Harvard Medical School, Boston Children's Hospital

JoVE 61677

Methods Collection

Automated Production of Human Induced Pluripotent Stem Cell-Derived Cortical and Dopaminergic Neurons with Integrated Live-Cell Monitoring

Ashutosh Dhingra*¹, Joachim Täger*¹, Elisangela Bressan*¹, Salvador Rodriguez-Nieto¹, Manmeet-Sakshi Bedi¹, Stefanie Bröer¹, Eldem Sadikoglou¹, Noémia Fernandes², Melissa Castillo-Lizardo¹, Patrizia Rizzu², Peter Heutink^1,3

¹Genome Biology of Neurodegenerative Diseases, German Center for Neurodegenerative Diseases (DZNE), ²Applied Genomics for Neurodegenerative Diseases, German Center for Neurodegenerative Diseases (DZNE), ³Department for Neurodegenerative Diseases, Hertie Institute for Clinical Brain Research, University of Tübingen

JoVE 61525

Methods Collection