JoVE Journal

Biology

JoVE Biology includes techniques and experimental approaches in the fields of cellular, molecular, and organismal biology.

‹1 2 3 4 5 6 7 8 9…88 › 2,110 VIDEO ARTICLES

Single Cell Durotaxis Assay for Assessing Mechanical Control of Cellular Movement and Related Signaling Events

Kathryn V. Svec^1,2,3, Johnathan B Patterson^1,2,3, Nyla Naim^1,2,3, Alan K Howe^1,2,3

¹Department of Pharmacology, University of Vermont Larner College of Medicine, ²University of Vermont Cancer Center, ³Department of Molecular Physiology and Biophysics, University of Vermont Larner College of Medicine

Using En Face Immunofluorescence Staining to Observe Vascular Endothelial Cells Directly

Chang Li*^1,2, Zhu Hui Liu*^1,2, Jia Wei Chen^1,2, Xin Yi Shu^1,2, Ying Shen¹, Feng Hua Ding¹, Rui Yan Zhang¹, Wei Feng Shen¹, Lin Lu¹, Xiao Qun Wang¹

¹Department of Cardiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, ²Institute of Cardiovascular Diseases, Shanghai Jiao Tong University

Isolation and Culture of Primary Aortic Endothelial Cells from Miniature Pigs

Yanli Zhao^1,2,3, Chengjiang Zhao⁴, David K.C. Cooper⁵, Ying Lu², Kewang Luo⁶, Huiyun Wang³, Pengfei Chen³, Changchun Zeng³, Shaodong Luan¹, Lisha Mou², Hanchao Gao^1,2,3

¹Department of Nephrology, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, ²Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen University School of Medicine, ³Department of Medical Laboratory, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, ⁴Department of Endocrinology, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, ⁵Xenotransplantation Program, Department of Surgery, University of Alabama at Birmingham, ⁶People's Hospital of Longhua

Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy

Christopher J. Guérin^1,2,3, Anna Kremer^1,2,3, Peter Borghgraef^1,2,3, Saskia Lippens^1,2,3

¹VIB Bio Imaging Core, ²VIB Inflammation Research Center, ³Department of Molecular Biomedical Research, UGent

Isolation of Mouse Epidermal Keratinocytes and Their In Vitro Clonogenic Culture

Rebecca J. Morris¹, Nyssa Readio¹, Kelsey Boland¹, Kelly Johnson¹, Sonali Lad¹, Anupama Singh¹, Ashok Singh¹, Stephanie Holtorf¹, Samantha Skaar¹

¹Hormel Institute, University of Minnesota

Visualizing Lymph Node Structure and Cellular Localization using Ex-Vivo Confocal Microscopy

Rafael M. Rezende*¹, Mateus E. Lopes*², Gustavo B Menezes², Howard L. Weiner¹

¹Ann Romney Center for Neurologic Diseases, Brigham and Women's Hospital, Harvard Medical School, ²Center for Gastrointestinal Biology, Federal University of Minas Gerais

Inducing Apical Periodontitis in Mice

Elisheva Goldman^1,2, Eli Reich¹, Itzhak Abramovitz*², Michael Klutstein*¹

¹Institute of Dental Sciences, Faculty of Dental Medicine, Hebrew University of Jerusalem, ²Department of Endodontics, Hadassah Ein Kerem Medical Center

A Labor-saving and Repeatable Touch-force Signaling Mutant Screen Protocol for the Study of Thigmomorphogenesis of a Model Plant Arabidopsis thaliana

Kai Wang¹, Kayin Law¹, Manhin Leung¹, Waishing Wong¹, Ning Li^1,2

¹Division of Life Science, Energy Institute, Institute for the Environment, The Hong Kong University of Science and Technology, ²Shenzhen Research Institute, The Hong Kong University of Science and Technology

In Vivo Surface Electrocardiography for Adult Zebrafish

Yali Zhao¹, Morgan Yun¹, Sean A. Nguyen¹, Michelle Tran¹, Thao P. Nguyen¹

¹Department of Medicine, Division of Cardiology, the Cardiovascular Research Laboratory, David Geffen School of Medicine at UCLA

Improving the Accuracy of Flow Cytometric Assessment of Mitochondrial Membrane Potential in Hematopoietic Stem and Progenitor Cells Through the Inhibition of Efflux Pumps

Claudia Morganti^1,2,3, Massimo Bonora^1,2,3, Keisuke Ito^1,2,3,4

¹Ruth L. and David S. Gottesman Institute for Stem Cell and Regenerative Medicine Research, Albert Einstein College of Medicine, ²Departments of Cell Biology and Stem Cell Institute, Albert Einstein College of Medicine, ³Department of Medicine, Albert Einstein College of Medicine, ⁴Albert Einstein Cancer Center and Diabetes Research Center, Albert Einstein College of Medicine

Simple Homemade Tools to Handle Fruit Flies—Drosophila melanogaster

¹Department of Zoology and Animal Physiology, College of Biological Sciences, China Agricultural University

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry

Shriram Venkatesan*¹, Tejbir S. Kandola*¹, Alejandro Rodríguez-Gama¹, Andrew Box¹, Randal Halfmann^1,2

¹Stowers Institute for Medical Research, ²Department of Molecular and Integrative Physiology, The University of Kansas School of Medicine

Identification and Dissection of Diverse Mouse Adipose Depots

Devika P. Bagchi¹, Ormond A. MacDougald¹

¹Department of Molecular & Integrative Physiology, University of Michigan Medical School

Photostimulation by Femtosecond Laser Activates Extracellular-signal-regulated Kinase (ERK) Signaling or Mitochondrial Events in Target Cells

Shaoyang Wang^1,2, Minglie Hu², Tao Ren¹, Hao He³

¹Department of Respiratory Medicine, Shanghai Sixth People's Hospital, School of Medicine, Shanghai Jiao Tong University, ²Ultrafast Laser Laboratory, College of Precision Instrument and Optoelectronics Engineering, Tianjin University, ³School of Biomedical Engineering, Shanghai Jiao Tong University

Fabrication of Amyloid-β-Secreting Alginate Microbeads for Use in Modelling Alzheimer's Disease

Bushra Almari¹, David Brough², Michael Harte¹, Annalisa Tirella¹

¹Division of Pharmacy and Optometry, School of Health Studies, Faculty of Biology, Medicine and Health, University of Manchester, ²Division of Neuroscience and Experimental Psychology, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester

Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans

Jean-Sébastien Teoh*¹, Ming S. Soh*¹, Joseph J. Byrne*¹, Brent Neumann¹

¹Neuroscience Program, Monash Biomedicine Discovery Institute and Department of Anatomy and Developmental Biology, Monash University, Melbourne VIC 3800, Australia.

SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs

Heike Fuhrmann-Stroissnigg*¹, Fernando E. Santiago*^1,2,3, Diego Grassi¹, YuanYuan Ling¹, Laura J. Niedernhofer^1,2,3, Paul D. Robbins^1,2,3

¹Department of Molecular Medicine and the Center on Aging, The Scripps Research Institute, ²Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, ³Institute on the Biology of Aging and Metabolism, University of Minnesota

Measuring Relative Insulin Secretion using a Co-Secreted Luciferase Surrogate

Michael Kalwat¹, Melanie H. Cobb¹

¹Department of Pharmacology, University of Texas Southwestern Medical Center

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Wilber Escorcia^1,2, Kuo-Fang Shen¹, Ji-Ping Yuan¹, Susan L. Forsburg¹

¹Program in Molecular and Computational Biology, University of Southern California, ²Leonard Davis School of Gerontology, University of Southern California

Isolation of Myoepithelial Cells from Adult Murine Lacrimal and Submandibular Glands

Tatiana Zyrianova¹, Liana V. Basova¹, Helen Makarenkova¹

¹Department of Molecular Medicine, The Scripps Research Institute

A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry

M. Edward Quach^1,2, Anum K. Syed^1,2, Renhao Li^1,2

¹Aflac Cancer and Blood Disorders Center, Children's Healthcare of Atlanta, ²Department of Pediatrics, Emory University School of Medicine

Using Microtiter Dish Radiolabeling for Multiple In Vivo Measurements Of Escherichia coli (p)ppGpp Followed by Thin Layer Chromatography

Llorenç Fernández-Coll¹, Michael Cashel¹

¹Intramural Research Program, Eunice Kennedy Shriver NICHD, NIH

Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers

Irina E. Catrina¹, Livia V. Bayer^1,2, Omar S. Omar^1,2, Diana P. Bratu^1,2

¹Biological Sciences Department Hunter College, City University of New York, ²Program in Molecular, Cellular, and Developmental Biology, Graduate Center, City University of New York

Assessment of Vascular Tone Responsiveness using Isolated Mesenteric Arteries with a Focus on Modulation by Perivascular Adipose Tissues

Daniels Konja¹, Cuiting Luo¹, Wai Yan Sun¹, Kangmin Yang¹, Andy W.C. Man¹, Aimin Xu¹, Paul M. Vanhoutte¹, Yu Wang¹

¹The State Key Laboratory of Pharmaceutical Biotechnology and the Department of Pharmacology and Pharmacy, University of Hong Kong

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