JoVE Journal

Biology

JoVE Biology publishes standard techniques and novel experimental approaches in the fields of cellular, molecular, and organismal biology aimed at understanding the functions of life and living organisms.

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Biophysical Characterization of Flagellar Motor Functions

Katie M. Ford¹, Ravi Chawla¹, Pushkar P. Lele¹

¹Artie McFerrin Department of Chemical Engineering, Texas A&M University

JoVE 55240

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Frederik Görlitz*¹, Douglas J. Kelly*¹, Sean C. Warren¹, Dominic Alibhai², Lucien West³, Sunil Kumar¹, Yuriy Alexandrov¹, Ian Munro¹, Edwin Garcia¹, James McGinty¹, Clifford Talbot¹, Remigiusz A. Serwa⁴, Emmanuelle Thinon⁴, Vincenzo da Paola³, Edward J. Murray⁵, Frank Stuhmeier⁶, Mark A. A. Neil¹, Edward W. Tate⁴, Christopher Dunsby^1,7, Paul M. W. French¹

¹Photonics Group, Department of Physics, Imperial College London, ²Institute for Chemical Biology, Department of Chemistry, Imperial College London, ³MRC Clinical Sciences Centre, Hammersmith Hospital, ⁴Chemical Biology Section, Department of Chemistry, Imperial College London, ⁵Retroscreen Virology Ltd, ⁶Pfizer Global Research and Development, Pfizer Limited, Sandwich, Kent, UK, ⁷Centre for Histopathology, Imperial College London

JoVE 55119

Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel

Daniel von Wangenheim¹, Robert Hauschild², Jiří Friml¹

¹Developmental and Cell Biology of Plants, Institute of Science and Technology Austria, ²Bioimaging Facility, Institute of Science and Technology Austria

JoVE 55044

Isolation and Characterization of Microvesicles from Peripheral Blood

Kerstin Menck¹, Annalen Bleckmann¹, Matthias Schulz¹, Lena Ries¹, Claudia Binder¹

¹Department of Hematology/Medical Oncology, University Medical Center Göttingen

JoVE 55057

Dissection of the Auditory Bulla in Postnatal Mice: Isolation of the Middle Ear Bones and Histological Analysis

Ayako Sakamoto¹, Yukiko Kuroda¹, Sho Kanzaki², Koichi Matsuo¹

¹Laboratory of Cell and Tissue Biology, Keio University School of Medicine, ²Department of Otolaryngology Head and Neck Surgery, Keio University School of Medicine

JoVE 55054

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix

Andrew L. Hellewell¹, Silvia Rosini¹, Josephine C. Adams¹

¹School of Biochemistry, University of Bristol

JoVE 55051

Studying Mitochondrial Structure and Function in Drosophila Ovaries

Danitra J Parker¹, Aida Moran¹, Kasturi Mitra¹

¹Department of Genetics, School of Medicine, University of Alabama at Birmingham

JoVE 54989

Xenopus Oocytes: Optimized Methods for Microinjection, Removal of Follicular Cell Layers, and Fast Solution Changes in Electrophysiological Experiments

Maria C. Maldifassi*¹, Nisa Wongsamitkul*¹, Roland Baur¹, Erwin Sigel¹

¹Institute of Biochemistry and Molecular Medicine, University of Bern

JoVE 55034

ACT-PRESTO: Biological Tissue Clearing and Immunolabeling Methods for Volume Imaging

Eunsoo Lee¹, Woong Sun¹

¹Department of Anatomy, Korea University College of Medicine

JoVE 54904

LED Thermo Flow — Combining Optogenetics with Flow Cytometry

Kathrin Brenker^1,2,3, Kerstin Osthof^1,4, Jianying Yang^1,3, Michael Reth^1,3,5

¹Max-Planck Institute for Immunobiology und Epigenetics, ²Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, ³Centre for Biological Signaling Studies, BIOSS, University of Freiburg, ⁴Albert-Ludwigs-Universität, ⁵Institute for Biology III (Mol. Immunology), Albert-Ludwigs-Universität

JoVE 54707

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

Snezana Vujicic*^1,2, Lanfei Feng*^1,2, Angelika Antoni³, Joyce Rauch⁴, Jerrold S. Levine^1,2,5

¹Section of Nephrology, Department of Medicine, University of Illinois at Chicago, ²Section of Nephrology, Department of Medicine, Jesse Brown Veterans Affairs Medical Center, ³Department of Biology, Kutztown University of Pennsylvania, ⁴Division of Rheumatology, Department of Medicine, Research Institute of the McGill University Health Centre, ⁵Department of Microbiology & Immunology, University of Illinois at Chicago

JoVE 54980

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Barbora Piknova¹, Ji Won Park¹, Katelyn S. Cassel¹, Cameron N. Gilliard¹, Alan N. Schechter¹

¹Molecular Medicine Branch, NIDDK, NIH

JoVE 54879

Observation and Quantification of Mating Behavior in the Pinewood Nematode, Bursaphelenchus xylophilus

Najie Zhu*¹, Liqun Bai*¹, Stefan Schütz², Baojun Liu³, Zhenyu Liu³, Xingyao Zhang⁴, Hongshi Yu¹, Jiafu Hu^1,5

¹Department of Forest Protection, Zhejiang Agricultural & Forestry University, ²Institute of Forest Zoology and Forest Conservation, Georg-August University Göttingen, ³College of Plant Protection, Shandong Agricultural University, ⁴Institute of Forest Protection, Chinese Academy of Forestry, ⁵Collaborative Innovation Center of Sustainable Forestry in Southern China, Nanjing Forestry University

JoVE 54842

Gap Junctional Intercellular Communication: A Functional Biomarker to Assess Adverse Effects of Toxicants and Toxins, and Health Benefits of Natural Products

Brad L. Upham¹, Iva Sovadinová², Pavel Babica²

¹Department of Pediatrics & Human Development, Institute for Integrative Toxicology, Michigan State University, ²RECETOX — Research Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University

JoVE 54281

The Use of Ex Vivo Whole-organ Imaging and Quantitative Tissue Histology to Determine the Bio-distribution of Fluorescently Labeled Molecules

Jeremy W. D. McGowan¹, Gene L. Bidwell, III¹

¹Department of Neurology, The University of Mississippi Medical Center

JoVE 54987

Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks

Michelle Bradley¹, Ivan Ramirez¹, Keith Cheung¹, Ankur A. Gholkar¹, Jorge Z. Torres^1,2,3

¹Department of Chemistry and Biochemistry, University of California, Los Angeles, ²Molecular Biology Institute, University of California, Los Angeles, ³Jonsson Comprehensive Cancer Center, University of California, Los Angeles

JoVE 54870

The ChIP-exo Method: Identifying Protein-DNA Interactions with Near Base Pair Precision

Andrea A. Perreault¹, Bryan J. Venters¹

¹Department of Molecular Physiology and Biophysics, Vanderbilt University

JoVE 55016

In Vivo Study of Human Endothelial-Pericyte Interaction Using the Matrix Gel Plug Assay in Mouse

Ke Yuan^1,2, Mark E. Orcholski^1,2, Ngan F. Huang^2,3, Vinicio A. de Jesus Perez^1,2

¹Division of Pulmonary and Critical Care Medicine, School of Medicine, Stanford University, ²Stanford Cardiovascular Institute, School of Medicine, Stanford University, ³VA Palo Alto Health Care System, Department of Cardiothoracic Surgery, School of Medicine, Stanford University

JoVE 54617

Isolation and Primary Culture of Mouse Aortic Endothelial Cells

Jie-Mei Wang¹, Alex F. Chen², Kezhong Zhang¹

¹Center for Molecular Medicine and Genetics, Wayne State University, ²Third Xiangya Hospital and the Institute of Vascular Disease and Translational Medicine, Central South University

JoVE 52965

Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses

Aaron M. Beedle¹

¹Department of Pharmaceutical and Biomedical Sciences, University of Georgia

JoVE 55058

Cultivation of Caenorhabditis elegans in Three Dimensions in the Laboratory

Tong Y. Lee¹, Kyoung-hye Yoon¹, Jin I. Lee¹

¹Division of Biological Science and Technology, Yonsei University

JoVE 55048

Dissection and Observation of Honey Bee Dorsal Vessel for Studies of Cardiac Function

Scott T. O'Neal¹, Troy D. Anderson¹

¹Department of Entomology, Virginia Tech

JoVE 55029

Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy (OCM)

Jing Men^1,2, Jason Jerwick^2,3, Penghe Wu^1,2, Mingming Chen^3,4, Aneesh Alex^2,3, Yutao Ma⁴, Rudolph E. Tanzi⁵, Airong Li⁵, Chao Zhou^1,2,3

¹Bioengineering Program, Lehigh University, ²Center for Photonics and Nanoelectronics, Lehigh University, ³Department of Electrical and Computer Engineering, Lehigh University, ⁴State Key Laboratory of Software Engineering, Wuhan University, ⁵Genetics and Aging Research Unit, Department of Neurology, Massachusetts General Hospital and Harvard Medical School

JoVE 55002

Study of Endoplasmic Reticulum and Mitochondria Interactions by In Situ Proximity Ligation Assay in Fixed Cells

Emily Tubbs¹, Jennifer Rieusset²

¹Lund University Diabetes Centre, Lund University, ²INSERM UMR-1060, CarMeN Laboratory, Lyon 1 University, INRA U1235, INSA of Lyon, Rockefeller and Charles Merieux Lyon-Sud Medical Universities

JoVE 54899

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RELATED SECTIONS

Cancer Research

Developmental Biology

Immunology and Infection

Neuroscience

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