JoVE Journal

Hussein Atta*^1,2, Ali Hashemi Gheinani*^1,2, Amanda Wacker¹, Yaser Heshmati^3,4,5, Alex Bigger-Allen^1,6, George Lambrinos^1,2, Yao Gao^2,7, Diane R. Bielenberg^2,7, Rosalyn M. Adam^1,2

¹Department of Urology, Boston Children's Hospital, ²Department of Surgery, Harvard Medical School, ³Division of Hematology/Oncology, Harvard Medical School Boston, ⁴Dana-Farber Cancer Institute, ⁵Broad Institute, ⁶Biological Biomedical Sciences Program, Division of Medical Sciences, Harvard Medical School, ⁷Vascular Biology Program, Boston Children's Hospital

JoVE 61455

Using a Thermal Camera to Measure Heat Loss Through Bird Feather Coats

Jordana M.F. Graveley¹, Kevin R. Burgio¹, Margaret Rubega¹

¹Department of Ecology and Evolutionary Biology, University of Connecticut

JoVE 60981

High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon

Atsushi Matsuda^1,2, Takako Koujin¹, Lothar Schermelleh³, Tokuko Haraguchi^1,2, Yasushi Hiraoka^1,2

¹Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, ²Graduate School of Frontier Biosciences, Osaka University, ³Micron Advanced Bioimaging Unit, Department of Biochemistry, University of Oxford

JoVE 60800

High-Throughput Assays of Critical Thermal Limits in Insects

David N. Awde¹, Tatum E. Fowler¹, Fernan Pérez-Gálvez¹, Mark J. Garcia¹, Nicholas M. Teets¹

¹Department of Entomology, University of Kentucky

JoVE 61186

Simultaneous Isolation and Culture of Atrial Myocytes, Ventricular Myocytes, and Non-Myocytes from an Adult Mouse Heart

Erik A. Blackwood¹, Alina S. Bilal¹, Khalid Azizi¹, Anup Sarakki¹, Christopher C. Glembotski¹

¹San Diego State University Heart Institute and the Department of Biology, San Diego State University

JoVE 61224

In Vivo Quantification of Protein Turnover in Aging C. Elegans using Photoconvertible Dendra2

Maria Lucia Pigazzini^1,2, Janine Kirstein^1,3

¹Leibniz Research Institute for Molecular Pharmacology im Forschungsverbund, ²NeuroCure Cluster of Excellence, Charité - Universitätsmedizin Berlin, ³Department of Biology and Chemistry, University of Bremen

JoVE 61196

Methods Collection

Single Cell Collection of Trophoblast Cells in Peri-implantation Stage Human Embryos

Deirdre M. Logsdon¹, Rebecca A. Kile¹, William B. Schoolcraft¹, Rebecca L. Krisher¹, Ye Yuan¹

¹Colorado Center for Reproductive Medicine

JoVE 61476

A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT

Janna M. Andronowski¹, Reed A. Davis¹, Caleb W. Holyoke²

¹Department of Biology, The University of Akron, ²Department of Geosciences, The University of Akron

JoVE 61081

Assessing the Age-Specific Phagocytic Ability of Adult Drosophila melanogaster Hemocytes using an In Vivo Phagocytosis Assay

Shonda M. Campbell¹, Michelle Starz-Gaiano¹, Jeff Leips¹

¹Department of Biology, University of Maryland Baltimore County

JoVE 60983

In Vivo Imaging of Transduction Efficiencies of Cardiac Targeting Peptide

Kyle S. Feldman¹, Maliha Zahid¹

¹Department of Developmental Biology, University of Pittsburgh School of Medicine

JoVE 60895

Measuring the Confluence of iPSCs using an Automated Imaging System.

Valentina Magliocca^1,2, Maria Vinci³, Tiziana Persichini², Franco Locatelli³, Marco Tartaglia¹, Claudia Compagnucci¹

¹Genetics and Rare Diseases Research Division, Ospedale Pediatrico Bambino Gesù, IRCCS, ²Department of Science, University Roma Tre, ³Department of Onco-hematology, Gene and Cell Therapy, Ospedale Pediatrico Bambino Gesù, IRCCS

JoVE 61225

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A)

Yohei Niikura*¹, Lei Fang*², Risa Kitagawa*³, Peizhao Li¹, Yao Xi¹, Ju You¹, Yan Guo², Katsumi Kitagawa³

¹MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, ²Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing University, ³Greehey Children's Cancer Institute, Department of Molecular Medicine, UT Health Science Center San Antonio

JoVE 61138

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

Daria Barwinska¹, Michael J. Ferkowicz¹, Ying-Hua Cheng¹, Seth Winfree^1,2, Kenneth W. Dunn¹, Katherine J. Kelly¹, Timothy A. Sutton¹, Brad H. Rovin³, Samir V. Parikh³, Carrie L. Phillips⁴, Pierre C. Dagher¹, Tarek M. El-Achkar¹, Michael T. Eadon¹, For The Kidney Precision Medicine Project,