JoVE Journal

Biology

JoVE Biology includes techniques and experimental approaches in the fields of cellular, molecular, and organismal biology.

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Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity

Adeline Unterreiner*¹, Ratiba Touil*¹, Daniele Anastasi¹, Nicolas Dubois¹, Satoru Niwa¹, Thomas Calzascia¹, Frederic Bornancin¹

¹Autoimmunity, Transplantation and Inflammation, Novartis Institutes for BioMedical Research

Isolation of CD4⁺ T-cells and Analysis of Circulating T-follicular Helper (cTfh) Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry

Elliot Byford¹, Matthew Carr¹, Lucia Piñon², Matthew J. Ahearne¹, Simon D. Wagner¹

¹Leicester Cancer Research Centre and Ernest and Helen Scott Haematology Research Institute, University of Leicester, ²MRC Toxicology Unit, University of Leicester

Reverse Dissection and DiceCT Reveal Otherwise Hidden Data in the Evolution of the Primate Face

Anne M. Burrows^1,2, Kailey M Omstead¹, Ashley R Deutsch^3,5, Justin T. Gladman⁴, Adam Hartstone-Rose³

¹Department of Physical Therapy, Duquesne University, ²Department of Anthropology, University of Pittsburgh, ³College of Sciences, North Carolina State University, ⁴Shared Materials Instrumentation Facility, Duke University, ⁵Department of Anthropology, University of Florida

The MUB₄₀ Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events

Mark C. Anderson^1,2, Louise Injarabian^1,2,3, Antonin Andre^1,2,3, Regis Tournebize^1,2,4, Benoit S. Marteyn^1,2

¹Unité de Pathogénie Microbienne Moléculaire, Institut Pasteur, ²INSERM Unité 1202, Institut Pasteur, ³Institut de Biochimie et Génétique Cellulaires, Université Bordeaux Segalen, ⁴Unit of Technology and Service Photonic BioImaging, Institut Pasteur

A Method for Islet Transplantation to the Omentum in Mouse

Ying Lu*¹, Shangyou Zou*¹, Suzanne Bertera*², Rita Bottino², David K.C. Cooper³, Zhengzhao Liu¹, Yi Huang¹, Chengjun Wang¹, Chungu Hong¹, Tian He¹, Hancheng Zhang¹, Qin Huo⁴, Hongxing Fu⁵, Zhiming Cai¹, Lisha Mou¹

¹Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen University School of Medicine, Shenzhen University Health Science Center, ²Institute for Cellular Therapeutics, Allegheny-Singer Research Institute, ³Xenotransplantation Program/Department of Surgery, The University of Alabama at Birmingham, ⁴College of Life Science and Oceanography, Shenzhen University, ⁵School of Pharmcy, Wenzhou Medical College

Bovine Mammary Gland Biopsy Techniques

Veridiana L. Daley^1,4, Charles Dye², Sophie H. Bogers³, R. Michael Akers⁴, Francisco C. Rodriguez³, John P. Cant⁵, John Doelman⁵, Peter Yoder⁴, Karan Kumar², Dane Webster⁶, Mark D. Hanigan⁴

¹National Animal Nutrition Program, a National Research Support Project (NRSP-9), Department of Animal and Food Sciences, University of Kentucky, ²School of Performing Arts, Virginia Tech, ³Department of Large Animal Clinical Sciences, The Virginia-Maryland College of Veterinary Medicine, Virginia Tech, ⁴Department of Dairy Science, Virginia Tech, ⁵Department of Animal Biosciences, University of Guelph, ⁶School of Visual Arts, Virginia Tech

Sperm Collection of Differential Quality Using Density Gradient Centrifugation

Muslah Uddin Ahammad*¹, Zachery Ryan Jarrell*¹, Andrew Parks Benson¹

¹Department of Poultry Science, University of Georgia

Effect of Fluorescent Proteins on Fusion Partners Using Polyglutamine Toxicity Assays in Yeast

Yuwei Jiang¹, Sonja Di Gregorio², Maram B. Albakri¹, Martin L. Duennwald^1,2, Patrick Lajoie¹

¹Department of Anatomy and Cell Biology, University of Western Ontario, ²Department of Pathology and Laboratory Medicine, University of Western Ontario

Synthesis of ⁶⁸Ga Core-doped Iron Oxide Nanoparticles for Dual Positron Emission Tomography /(T₁)Magnetic Resonance Imaging

Irene Fernández-Barahona¹, Jesús Ruiz-Cabello², Fernando Herranz¹, Juan Pellico³

¹Nanobiotechnology, Molecular Imaging and Metabolomics Lab, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), ²CIC biomaGUNE and CIBER de Enfermedades Respiratorias (CIBERES). Ikerbasque, Basque Foundation for Science, Universidad Complutense de Madrid (UCM), ³Advanced Imaging Unit, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), CIBER de Enfermedades Respiratorias (CIBERES)

Visualization of 3D White Adipose Tissue Structure Using Whole-mount Staining

Yanqing Jiang*¹, Joanna Lan-Hing Yeung*¹, Ju Hee Lee^1,2, James An¹, Patrick E. Steadman³, Jae-Ryong Kim⁴, Hoon-Ki Sung^1,2,5

¹Translational Medicine Program, The Hospital for Sick Children, ²Department of Laboratory Medicine and Pathobiology, University of Toronto, ³Neurosciences & Mental Health Program, The Hospital for Sick Children, ⁴Department of Biochemistry and Molecular Biology, Smart-Aging Convergence Research Center, College of Medicine, Yeungnam University, ⁵Banting and Best Diabetes Centre, University of Toronto

LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring

Portia Ritter*^1,2, Keyvan Yousefi*^1,3, Juliana Ramirez⁴, Derek M. Dykxhoorn^4,5, Armando J. Mendez⁶, Lina A. Shehadeh^1,2,7,8

¹Interdisciplinary Stem Cell Institute, University of Miami Leonard M. Miller School of Medicine, ²Department of Medicine, Division of Cardiology, University of Miami Leonard M. Miller School of Medicine, ³Department of Molecular and Cellular Pharmacology, University of Miami Leonard M. Miller School of Medicine, ⁴Dr. John T. Macdonald Foundation Department of Human Genetics, University of Miami Leonard M. Miller School of Medicine, ⁵John P. Hussman Institute for Human Genomics, University of Miami Leonard M. Miller School of Medicine, ⁶Department of Medicine, Division of Endocrinology, Metabolism and Endocrinology and the Diabetes Research Institute, University of Miami Leonard M. Miller School of Medicine, ⁷Vascular Biology Institute, University of Miami Leonard M. Miller School of Medicine, ⁸Peggy and Harold Katz Family Drug Discovery Center, University of Miami Leonard M. Miller School of Medicine

Laser-assisted Lentiviral Gene Delivery to Mouse Fertilized Eggs

Negin P. Martin*¹, Page Myers*², Eugenia Goulding¹, Shih-Heng Chen¹, Mitzie Walker¹, Thomas M. Porter¹, Lucas Van Gorder¹, Amanda Mathew¹, Artiom Gruzdev³, Erica Scappini⁴, Charles Romeo¹

¹Neurobiology Laboratory, National Institute of Environmental Health Sciences, ²Comparative Medicine Branch, National Institute of Environmental Health Sciences, ³Reproductive and Developmental Biology Laboratory, National Institute of Environmental Health Sciences, ⁴Signal Transduction Laboratory, National Institute of Environmental Health Sciences

Expression of Fluorescent Fusion Proteins in Murine Bone Marrow-derived Dendritic Cells and Macrophages

Jarmila Kralova^1,2, Daniela Glatzova^1,2,3, Simon Borna^1,2, Tomas Brdicka¹

¹Laboratory of Leukocyte Signalling, Institute of Molecular Genetics of the ASCR, ²Faculty of Science, Charles University, ³Department of Biophysical Chemistry, J. Heyrovsky Institute of Physical Chemistry ASCR

A Plate Competition Assay As a Quick Preliminary Assessment of Disease Suppression

Deborah A. Neher¹, Thomas R. Weicht¹

¹Department of Plant and Soil Science, University of Vermont

Combined Genetic and Chemical Capsid Modifications of Adenovirus-Based Gene Transfer Vectors for Shielding and Targeting

Franziska Jönsson¹, Claudia Hagedorn¹, Florian Kreppel¹

¹Center of Biomedical Education and Research, University Witten/Herdecke

Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples

Ji-Yeon Hyeon¹, David A. Mann¹, Anna M. Townsend¹, Xiangyu Deng¹

¹Center for Food Safety

High-throughput Measurement of Gut Transit Time Using Larval Zebrafish

Steven Cassar¹, Xin Huang², Todd Cole³

¹Preclinical Safety, AbbVie, ²Statistics, AbbVie, ³Integrated Science and Technology, AbbVie

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability

Ban-Ruo Li*^1,2, Jia Wu*^1,2, Hua-Shan Li^1,2, Zhi-Hui Jiang^1,2, Xiu-Min Zhou^1,2, Cai-Hua Xu^1,2, Ning Ding^1,2, Juan-Min Zha^1,2, Wei-Qi He^1,2

¹Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genome Resource Center, Soochow University, ²Department of Oncology, The First Affiliated Hospital of Soochow University

Growing Magnetotactic Bacteria of the Genus Magnetospirillum: Strains MSR-1, AMB-1 and MS-1

Lucas Le Nagard¹, Viviana Morillo-López², Cecile Fradin¹, Dennis A. Bazylinski²

¹Department of Physics & Astronomy, McMaster University, ²School of Life Sciences, University of Nevada Las Vegas

Determination of Reproductive Competence by Confirming Pubertal Onset and Performing a Fertility Assay in Mice and Rats

Hanne M. Hoffmann¹

¹Department of Animal Science, Michigan State University

Micropuncture of Bowman's Space in Mice Facilitated by 2 Photon Microscopy

Katsuyuki Matsushita¹, Kirsti Golgotiu¹, Daniel J. Orton², Richard D. Smith², Karin D. Rodland², Paul D. Piehowski², Michael P. Hutchens^1,3

¹Anesthesiology & Perioperative Medicine, Oregon Health & Science University, ²Environmental and Biological Services Division, Pacific Northwest National Laboratory, ³Operative Care Division, Portland Veterans Affairs Medical Center

Micron-scale Phenotyping Techniques of Maize Vascular Bundles Based on X-ray Microcomputed Tomography

Ying Zhang*¹, Liming Ma*¹, Xiaodi Pan*¹, Jinglu Wang¹, Xinyu Guo¹, Jianjun Du¹

¹Beijing Key Lab of Digital Plant, Beijing Research Center for Information Technology in Agriculture, Beijing Academy of Agriculture and Forestry Sciences

Retinal Cryo-sections, Whole-Mounts, and Hypotonic Isolated Vasculature Preparations for Immunohistochemical Visualization of Microvascular Pericytes

Karin Dreisig¹, Frank Wojciechowski Blixt², Karin Warfvinge^1,2

¹Department of Clinical Experimental Research, Glostrup Research Institute, ²Department of Clinical Sciences, Division of Experimental Vascular Research, Lund University

A Versatile Model of Hard Tick Infestation on Laboratory Rabbits

Consuelo Almazán¹, Sarah Bonnet¹, Martine Cote¹, Mirko Slovák², Yoonseong Park³, Ladislav Šimo¹

¹Unité Mixte de Recherche (UMR) Biologie moléculaire et immunologie parasitaires (BIPAR), Institut national de la recherche agronomique (INRA), Ecole Nationale Vétérinaire d'Alfort, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Université Paris-Est, ²Institute of Zoology, Slovak Academy of Sciences, ³Department of Entomology, Kansas State University

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