JoVE Journal

Biology

JoVE Biology publishes standard techniques and novel experimental approaches in the fields of cellular, molecular, and organismal biology aimed at understanding the functions of life and living organisms.

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High-resolution Optical Mapping of the Mouse Sino-atrial Node

Di Lang¹, Alexey V. Glukhov¹

¹Department of Medicine, University of Wisconsin-Madison School of Medicine and Public Health

JoVE 54773

Kinematic Analysis of Cell Division and Expansion: Quantifying the Cellular Basis of Growth and Sampling Developmental Zones in Zea mays Leaves

Katrien Sprangers*¹, Viktoriya Avramova*¹, Gerrit T. S. Beemster¹

¹Department of Biology, University of Antwerp

JoVE 54887

Studying the Protein Quality Control System of D. discoideum Using Temperature-controlled Live Cell Imaging

Liliana Malinovska¹, Simon Alberti¹

¹Max-Planck Institute for Molecular Cell Biology and Genetics

JoVE 54730

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)

Yilin Wang¹, Pakorn Kanchanawong^2,3

¹Department of Biology, South University of Science and Technology of China, Shenzhen, ²Mechanobiology Institute, Singapore, ³Department of Biomedical Engineering, National University of Singapore

JoVE 54774

In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila

Ho Lam Tang¹, Ho Man Tang¹, Ming Chiu Fung², J. Marie Hardwick¹

¹W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins University Bloomberg School of Public Health, ²School of Life Sciences, Chinese University of Hong Kong

JoVE 53992

Hybrid-Cut: An Improved Sectioning Method for Recalcitrant Plant Tissue Samples

Tien-Kuan Chen*¹, Hui-Ting Yang*¹, Su-Chiung Fang¹, Yi-Chen Lien¹, Ting-Ting Yang¹, Swee-Suak Ko¹

¹Academia Sinica Biotechnology Center in Southern Taiwan; Agricultural Biotechnology Research Center, Academia Sinica

JoVE 54754

Visualizing Stromule Frequency with Fluorescence Microscopy

Jacob O. Brunkard¹, Anne M. Runkel², Patricia Zambryski²

¹Plant Gene Expression Center, Agricultural Research Service, USDA, ²Department of Plant and Microbial Biology, University of California, Berkeley

JoVE 54692

Automated Quantification and Analysis of Cell Counting Procedures Using ImageJ Plugins

Jacob O'Brien¹, Heyam Hayder¹, Chun Peng¹

¹Department of Biology, York University

JoVE 54719

The Mouse Isolated Perfused Kidney Technique

Jan Czogalla¹, Frank Schweda², Johannes Loffing¹

¹Institute of Anatomy, Swiss National Centre of Competence in Research Kidney, University of Zürich, ²Department of Physiology, University of Regensburg

JoVE 54712

A Method for Extracting Pigments from Squid Doryteuthis pealeii

Christopher W. DiBona¹, Thomas L. Williams¹, Sean R. Dinneen¹, Stephanie F. Jones Labadie¹, Leila F. Deravi^1,2

¹Department of Chemistry, University of New Hampshire, ²Materials Science Program, University of New Hampshire

JoVE 54803

Analysis of SCAP N-glycosylation and Trafficking in Human Cells

Chunming Cheng¹, Jeffrey Yunhua Guo¹, Feng Geng¹, Xiaoning Wu¹, Xiang Cheng¹, Qiyue Li¹, Deliang Guo¹

¹Department of Radiation Oncology, The Ohio State University Comprehensive Cancer Center and College of Medicine

JoVE 54709

A Buoyancy-based Method of Determining Fat Levels in Drosophila

Kelsey E. Hazegh¹, Tânia Reis¹

¹Department of Medicine, Division of Endocrinology, Metabolism, and Diabetes, University of Colorado Anschutz Medical Campus

JoVE 54744

Analysis of Protein Import into Chloroplasts Isolated from Stressed Plants

Qihua Ling¹, Paul Jarvis¹

¹Department of Plant Sciences, University of Oxford

JoVE 54717

Techniques for Imaging Prometaphase and Metaphase of Meiosis I in Fixed Drosophila Oocytes

Sarah J. Radford¹, Kim S. McKim^1,2

¹Waksman Institute, Rutgers University, ²Department of Genetics, Rutgers University

JoVE 54666

The Assembly and Application of 'Shear Rings': A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress

Luke A. White¹, Emily V. Stevenson¹, J. Winny Yun¹, Randa Eshaq¹, Norman R. Harris¹, David K. Mills², Alireza Minagar³, Pierre-Olivier Couraud⁴, J. Steven Alexander¹

¹Molecular and Cellular Physiology, Louisiana State University Health Sciences Center in Shreveport, ²Biological Sciences, Louisiana Tech University, ³Neurology, Louisiana State University Health Sciences Center in Shreveport, ⁴Institut Cochin, Inserm U1016, Cnrs Umr8104, Université Paris Descartes

JoVE 54632

High-fat Feeding Paradigm for Larval Zebrafish: Feeding, Live Imaging, and Quantification of Food Intake

Jessica P. Otis¹, Steven A. Farber^1,2

¹Department of Embryology, Carnegie Institution for Science, ²Department of Biology, Johns Hopkins University

JoVE 54735

Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast

Derek C. Prosser¹, Kristie Wrasman¹, Thaddeus K. Woodard¹, Allyson F. O’Donnell², Beverly Wendland¹

¹Department of Biology, The Johns Hopkins University, ²Department of Biological Sciences, Duquesne University

JoVE 54587

Methods for the Isolation, Culture, and Functional Characterization of Sinoatrial Node Myocytes from Adult Mice

Emily J. Sharpe*¹, Joshua R. St. Clair*^1,2, Catherine Proenza^1,3

¹Department of Physiology and Biophysics, University of Colorado Anschutz Medical Campus, ²Department of Bioengineering, University of Colorado Anschutz Medical Campus, ³Department of Medicine, Division of Cardiology, University of Colorado Anschutz Medical Campus

JoVE 54555

Measuring the Stiffness of Ex Vivo Mouse Aortas Using Atomic Force Microscopy

Yong Ho Bae^1,2, Shu-lin Liu^1,2, Fitzroy J. Byfield³, Paul A. Janmey³, Richard K. Assoian^1,2

¹Program in Translational Biomechanics, Institute of Translational Medicine and Therapeutics, University of Pennsylvania, ²Department of Systems Pharmacology and Translational Therapeutics, University of Pennsylvania, ³Department of Physiology, University of Pennsylvania

JoVE 54630

Visualization and Quantification of the Cell-free Layer in Arterioles of the Rat Cremaster Muscle

Yan Cheng Ng^1,2, Liam K. Fisher², Veena Salim², Sangho Kim^1,2,3, Bumseok Namgung²

¹NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, ²Department of Biomedical Engineering, National University of Singapore, ³Department of Surgery, National University of Singapore

JoVE 54550

An Alternative Approach for Sample Preparation with Low Cell Number for TEM Analysis

Sachin Kumar¹, Marie-Dominique Filippi¹

¹Division of Experimental Hematology and Cancer Biology, Cincinnati Children’s Research Foundation

JoVE 54724

Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry

Benoîte Bourdin¹, Emilie Segura¹, Marie-Philippe Tétreault¹, Sylvie Lesage², Lucie Parent¹

¹Département de Physiologie Moléculaire et Intégrative, Montreal Heart Institute Research Centre, ²Département de Microbiologie, Infectiologie, Immunologie, Centre de recherche de l'Hôpital Maisonneuve-Rosemont

JoVE 54732

Methods for the Determination of Rates of Glucose and Fatty Acid Oxidation in the Isolated Working Rat Heart

Bhavisha Bakrania, Joey P. Granger, Romain Harmancey

¹Department of Physiology and Biophysics, Mississippi Center for Obesity Research, Cardiovascular-Renal Research Center, University of Mississippi Medical Center

JoVE 54497

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter

Louise L. Hansen¹, Gerben van Ooijen¹

¹Institute for Molecular Plant Sciences, University of Edinburgh

JoVE 54586

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Cancer Research

Developmental Biology

Immunology and Infection

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