JoVE Journal

Biology

JoVE Biology includes techniques and experimental approaches in the fields of cellular, molecular, and organismal biology.

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Modified Methods for Loading of High-Throughput DNA Extraction Plates Reduce Potential for Contamination

Gordon F. Custer*^1,2, Reilly R. Dibner*^3,4

¹Department of Ecosystem Science and Management, University of Wyoming, ²Program in Ecology, University of Wyoming, ³EPSCoR-IDEA, University of Wyoming, ⁴Department of Botany, University of Wyoming

JoVE 61405

Circadian Entrainment of Drosophila Melanogaster

Austin O. Dada¹, Minh Q. Nguyen¹, Shea M. Peterson¹, Vy T. Ngo¹, Dayanne V. Cornelio-Parra¹, Bwaar S. Omer¹, Ada Thapa², Sarah R. Rapp¹, Veronica J. Cloud¹, Ryan D. Mohan¹

¹School of Biological and Chemical Sciences, University of Missouri - Kansas City, ²School of Public Health, University of Maryland

JoVE 61176

Obtaining Primary Osteocytes through Murine Calvarial Fractionation of GFP-Expressing Osteocytes

Aseel Marahleh¹, Hideki Kitaura¹, Saika Ogawa¹, Wei-Ren Shen¹, Jiawei Qi¹, Fumitoshi Ohori¹, Takahiro Noguchi¹, Yasuhiko Nara¹, Adya Pramusita¹, Ria Kinjo¹, Itaru Mizoguchi¹

¹Division of Orthodontics and Dentofacial Orthopedics, Department of Translational Medicine, Tohoku University Graduate School of Dentistry

JoVE 61513

Fluorescence-Activated Cell Sorting for the Isolation of Scleractinian Cell Populations

Grace A. Snyder¹, William E. Browne², Nikki Traylor-Knowles*¹, Benyamin Rosental*³

¹Department of Marine Biology and Ecology, Rosenstiel School of Marine and Atmospheric Science, University of Miami, ²Department of Biology, University of Miami, ³The Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, and Regenerative Medicine and Stem Cell Research Center, Ben Gurion University of the Negev

JoVE 60446

Worm-align and Worm_CP, Two Open-Source Pipelines for Straightening and Quantification of Fluorescence Image Data Obtained from Caenorhabditis elegans

Hanneke Okkenhaug¹, Laetitia Chauve¹, Fatemeh Masoudzadeh¹, Lars Okkenhaug¹, Olivia Casanueva¹

¹Babraham Institute

JoVE 61136

Methods Collection

Reconstruction of Single-Cell Innate Fluorescence Signatures by Confocal Microscopy

Tomohiro Hirayama*¹, Kyosuke Takabe*², Tatsunori Kiyokawa¹, Nobuhiko Nomura^2,3, Yutaka Yawata*^2,3

¹Graduate School of Life and Environmental Sciences, University of Tsukuba, ²Faculty of Life and Environmental Sciences, University of Tsukuba, ³Microbiology Research Center for Sustainability, University of Tsukuba

JoVE 61120

Time-lapse Imaging of Bacterial Swarms and the Collective Stress Response

Jean-Louis Bru¹, Albert Siryaporn^1,2, Nina Molin Høyland-Kroghsbo³

¹Department of Molecular Biology & Biochemistry, University of California, Irvine, ²Department of Physics & Astronomy, University of California, Irvine, ³Department of Veterinary and Animal Sciences, University of Copenhagen

JoVE 60915

A Pipeline using Bilateral In Utero Electroporation to Interrogate Genetic Influences on Rodent Behavior

Ashley L. Comer^1,2,3,4, Balaji Sriram⁵, William W. Yen², Alberto Cruz-Martín^1,2,3,4,6

¹The Graduate Program for Neuroscience, Boston University, ²Department of Biology, Boston University, ³Neurophotonics Center, Boston University, ⁴Center for Systems Neuroscience, Boston University, ⁵Research and Early Development, Biogen, ⁶Department Pharmacology and Experimental Therapeutics, Boston University

JoVE 61350

Methods Collection

Measurements of Physiological Stress Responses in C. Elegans

Raz Bar-Ziv*¹, Ashley E. Frakes*¹, Ryo Higuchi-Sanabria*¹, Theodore Bolas¹, Phillip A. Frankino¹, Holly K. Gildea¹, Melissa G. Metcalf¹, Andrew Dillin¹

¹Department of Molecular and Cell Biology, University of California, Berkeley

JoVE 61001

Methods Collection

Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation

Kelly J. Domesle¹, Shenia R. Young¹, Qianru Yang¹, Beilei Ge¹

¹Center for Veterinary Medicine, U.S. Food and Drug Administration

JoVE 61239

Tissue-Specific RNAi Tools to Identify Components for Systemic Stress Signaling

Jay Miles¹, Patricija van Oosten-Hawle¹

¹School of Molecular and Cell Biology & Astbury Centre for Structural Molecular Biology, University of Leeds

JoVE 61357

Methods Collection

Isolation and Characterization of Exosomes from Skeletal Muscle Fibroblasts

Diantha van de Vlekkert¹, Xiaohui Qiu¹, Ida Annunziata¹, Alessandra d'Azzo¹

¹Department of Genetics, St. Jude Children's Research Hospital

JoVE 61127

Simple Detection of Primary Cilia by Immunofluorescence

Alzbeta Filipova¹, Daniel Diaz Garcia², Josef Dvorak³, Stanislav Filip⁴, Marcela Jelicova¹, Zuzana Sinkorova¹

¹Department of Radiobiology, Faculty of Military Health Sciences in Hradec Kralove, University of Defence, ²Department of Clinical Biochemistry and Diagnostics, University Hospital, ³Department of Oncology, Thomayer Hospital, Charles University, ⁴Department of Oncology and Radiotherapy, Faculty of Medicine, Charles University

JoVE 61155

An Air-liquid Interface Bronchial Epithelial Model for Realistic, Repeated Inhalation Exposure to Airborne Particles for Toxicity Testing

Hedwig M. Braakhuis¹, Ruiwen He^1,2, Rob J. Vandebriel¹, Eric R. Gremmer¹, Edwin Zwart¹, Jolanda P. Vermeulen¹, Paul Fokkens¹, John Boere¹, Ilse Gosens¹, Flemming R. Cassee^1,2

¹National Institute for Public Health and the Environment (RIVM), ²Institute for Risk Assessment Sciences (IRAS)

JoVE 61210

Methods Collection

Investigating Flagella-Driven Motility in Escherichia coli by Applying Three Established Techniques in a Series

Jonathan D. Partridge¹, Rasika M. Harshey¹

¹Department of Molecular Biosciences, The University of Texas at Austin

JoVE 61364

Hepatic Progenitor Specification from Pluripotent Stem Cells using a Defined Differentiation System

Jose Meseguer-Ripolles¹, Yu Wang¹, Agnes Sorteberg¹, Aishwariya Sharma², Nan-Linda Ding², Baltasar Lucendo-Villarin¹, Philipp Kramer², Charis-Patricia Segeritz², David C. Hay¹

¹MRC Centre for Regenerative Medicine, University of Edinburgh, ²Research & Development, STEMCELL Technologies Inc

JoVE 61256

Methods Collection