JoVE Journal

Biology

JoVE Biology includes techniques and experimental approaches in the fields of cellular, molecular, and organismal biology.

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High-throughput Screening for Protein-based Inheritance in S. cerevisiae

James S. Byers¹, Daniel F. Jarosz^1,2

¹Department of Developmental Biology, Stanford University School of Medicine, ²Department of Chemical and Systems Biology, Stanford University School of Medicine

A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice

Chrstine Bäuerl*¹, Özgun C.O. Umu*², Pablo E. Hernandez³, Dzung B. Diep⁴, Gaspar Pérez-Martínez¹

¹Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), ²Department of Food Safety and Infection Biology, Norwegian University of Life Sciences (NMBU), ³Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid (UCM), ⁴Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU)

A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling

Naeem Nathoo^1,2, Mark A. Bernards², Jacqueline MacDonald³, Ze-Chun Yuan^1,3

¹London Research and Development Centre, Agriculture & Agri-Food Canada, ²Department of Biology, University of Western Ontario, ³Department of Microbiology and Immunology, University of Western Ontario

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry

Haley R. Pugsley¹

¹Amnis Biology Department, MilliporeSigma

Determination of Sialic Acids in Liver and Milk Samples of Wild-type and CMAH Knock-out Mice.

Cui Cao¹, Wen J. Wang¹, Ying Y. Huang¹, Hong L. Yao¹, Louis P. Conway¹, Li Liu¹, Josef Voglmeir¹

¹Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University

Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin

Fengwu Li¹, Christopher A. Adase¹, Ling-juan Zhang¹

¹Department of Dermatology, School of Medicine, UC San Diego

A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions

Tanumoy Saha¹, Isabel Rathmann¹, Milos Galic¹

¹DFG Cluster of Excellence 'Cells in Motion', (EXC 1003), Institute of Medical Physics and Biophysics, University of Muenster

Live-cell Imaging of Platelet Degranulation and Secretion Under Flow

Arjan D. Barendrecht*¹, Johan J. F. Verhoef*², Silvia Pignatelli¹, Gerard Pasterkamp¹, Harry F. G. Heijnen¹, Coen Maas¹

¹Department of Clinical Chemistry and Haematology, University Medical Center Utrecht, ²Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Utrecht University

ODELAY: A Large-scale Method for Multi-parameter Quantification of Yeast Growth

Thurston Herricks¹, Fred D. Mast^1,2, Song Li¹, John D. Aitchison^1,2

¹Institute for Systems Biology, ²Center for Infectious Disease Research

Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques

Colleen M. Garvey¹, Torin A. Gerhart¹, Shannon M. Mumenthaler¹

¹Lawrence J. Ellison Institute for Transformative Medicine, University of Southern California (USC)

Continuous-wave Thulium Laser for Heating Cultured Cells to Investigate Cellular Thermal Effects

Yoko Miura^1,2,3, Joachim Pruessner¹, Carla Lotta Mertineit¹, Katharina Kern¹, Michael Muenter¹, Moritz Moltmann², Veit Danicke², Ralf Brinkmann^1,2

¹Institute of Biomedical Optics, University of Luebeck, ²Medical Laser Center Luebeck GmbH, University of Leubeck, ³Department of Ophthalmology, University of Luebeck

Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes

Stephanie Chanon¹, Christine Durand¹, Aurelie Vieille-Marchiset¹, Maud Robert², Charna Dibner³, Chantal Simon¹, Etienne Lefai¹

¹CarMeN Laboratory, INSERM U1060, INRA 1397, University of Lyon, ²Department of digestive and bariatric surgery, Obesity Integrated Center, University Hospital of Edouard Herriot, Hospices Civils de Lyon, Lyon 1 University, ³Division of Endocrinology, Diabetes, Hypertension and Nutrition, Department of Clinical Medicine, Faculty of Medicine, University of Geneva

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

Orlando Santillán¹, Miguel A. Ramírez-Romero², Guillermo Dávila³

¹Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, ²Oncomedic Incorporation, Ciudad de México, ³Laboratorio Internacional de Investigación sobre el Genoma Humano, Universidad Nacional Autónoma de México

Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy

Heiko Lemcke^1,2,3, Natalia Voronina^1,2,3, Gustav Steinhoff^1,2,3, Robert David^1,2,3

¹Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), ²Department of Cardiac Surgery, University of Rostock, ³Department of Life, Light and Matter of the Interdisciplinary Faculty, University of Rostock

Clock Scan Protocol for Image Analysis: ImageJ Plugins

Maxim Dobretsov¹, Georg Petkau², Abdallah Hayar³, Eugen Petkau

¹Department of Anesthesiology, University of Arkansas for Medical Sciences, ²Department of Lymphocyte Development, Max Plank Institute for Infection Biology, ³Department of Neurobiology & Developmental Sciences, University of Arkansas for Medical Sciences

Methods for the Extraction of Endosymbionts from the Whitefly Bemisia tabaci

Dan-Tong Zhu¹, Xin-Ru Wang¹, Fei-Xue Ban¹, Chi Zou¹, Shu-Sheng Liu¹, Xiao-Wei Wang¹

¹Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogen and Insects, Institute of Insect Sciences, Zhejiang University

DiI Perfusion as a Method for Vascular Visualization in Ambystoma mexicanum

Anna J. Saltman*^1,2, May Barakat*¹, Donald M. Bryant¹, Anastasia Brodovskaya¹, Jessica L. Whited¹

¹Department of Orthopedic Surgery, Harvard Medical School and Brigham and Women's Hospital, ²Division of Graduate Medical Sciences, Boston University

Using a Whole-mount Immunohistochemical Method to Study the Innervation of the Biliary Tract in Suncus murinus

Ke Ren¹, Yidan Dai¹, Kai Yi², Masanobu Kinoshita¹, Masahiro Itoh², Ichiro Sakata³, Takafumi Sakai³, Shuang-Qin Yi¹

¹Department of Frontier Health Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, ²Department of Anatomy, Tokyo Medical University, ³Area of Regulatory Biology, Division of Life Science, Graduate School of Science and Engineering, Saitama University

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells

Francesco Castagnetti^1,2, Elisabetta Fiacco³, Carol Imbriano⁴, Lucia Latella^1,2

¹Department of Medicine, Institute of Translational Pharmacology, Italian National Research Council, ²Epigenetics and Regenerative Medicine, IRCCS Fondazione Santa Lucia, ³Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), ⁴Department of Life Sciences, University of Modena and Reggio Emilia

Murine Aortic Crush Injury: An Efficient In Vivo Model of Smooth Muscle Cell Proliferation and Endothelial Function

Dan Yu^1,4, George Makkar², Rajabrata Sarkar^2,3,4, Dudley K. Strickland^2,3,4, Thomas S. Monahan^1,2,4

¹Department of Surgery, Baltimore Veterans Affairs Medical Center, ²Department of Surgery, University of Maryland School of Medicine, ³Department of Physiology, University of Maryland School of Medicine, ⁴Center for Vascular and Inflammatory Diseases, University of Maryland School of Medicine

Monitoring ER/SR Calcium Release with the Targeted Ca²⁺ Sensor CatchER⁺

Florence N. Reddish¹, Cassandra L. Miller¹, Rakshya Gorkhali¹, Jenny J. Yang¹

¹Department of Chemistry, Center of Diagnostics and Therapeutics (CDT), Georgia State University

Simultaneous Isolation of High Quality Cardiomyocytes, Endothelial Cells, and Fibroblasts from an Adult Rat Heart

Dursun Gündüz, Christian W. Hamm, Muhammad Aslam

¹Internal Medicine, Cardiology and Angiology, University Hospital Giessen

En Face Preparation of Mouse Blood Vessels

Kyung Ae Ko¹, Keigi Fujiwara¹, Sunil Krishnan², Jun-ichi Abe¹

¹Department of Cardiology, Division of Internal Medicine, University of Texas MD Anderson Cancer Center, ²Department of Radiation Oncology, University of Texas MD Anderson Cancer Center

Cellular Redox Profiling Using High-content Microscopy

Tom Sieprath^1,2, Tobias Corne^1,2, Joke Robijns¹, Werner J. H. Koopman³, Winnok H. De Vos^1,2

¹Laboratory of Cell Biology and Histology, Department of Veterinary Sciences, University of Antwerp, ²Cell Systems and Imaging Research Group (CSI), Department of Molecular Biotechnology, Ghent University, ³Department of Biochemistry , Radboud Institute for Molecular Life Sciences, Radboud University Medical Center

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