JoVE Journal

Biology

JoVE Biology publishes standard techniques and novel experimental approaches in the fields of cellular, molecular, and organismal biology aimed at understanding the functions of life and living organisms.

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Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Haseong Kim¹, Kil Koang Kwon¹, Wonjae Seong¹, Seung-Goo Lee^1,2

¹Synthetic Biology & Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology, ²Biosystems and Bioengineering Program, University of Science and Technology, Daejeon, South Korea

JoVE 54059

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

Sally M. McFall¹, Mário F. Neto¹, Jennifer L. Reed¹, Robin L. Wagner²

¹Center for Innovation in Global Health Technologies (CIGHT), Department of Biomedical Engineering, Northwestern University, ²Pritzker School of Medicine, The University of Chicago

JoVE 54289

CUBIC Protocol Visualizes Protein Expression at Single Cell Resolution in Whole Mount Skin Preparations

Huazheng Liang*^1,2, Bassem Akladios*¹, Cesar P. Canales*¹, Richard Francis³, Edna H. Hardeman¹, Annemiek Beverdam¹

¹The School of Medical Sciences, University of New South Wales Australia, ²Neuroscience Research Australia, ³Biomedical Imaging Facility, University of New South Wales Australia

JoVE 54401

Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization (FISH) with PNA Probes in Caenorhabditis elegans

Beomseok Seo¹, Junho Lee^1,2,3

¹Institute of Molecular Biology and Genetics (IMBG), Seoul National University, ²Department of Biological Sciences, Seoul National University, ³Department of Biophysics and Chemical Biology, Seoul National University

JoVE 54224

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Dorrelyn P. Martin¹, Jharna Miya¹, Julie W. Reeser¹, Sameek Roychowdhury^1,2

¹Department of Internal Medicine, Division of Medical Oncology, Comprehensive Cancer Center, The Ohio State University, ²Department of Pharmacology, The Ohio State University

JoVE 54090

Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System

Emanuel G. Worst¹, Matthias P. Exner², Alessandro De Simone², Marc Schenkelberger¹, Vincent Noireaux³, Nediljko Budisa², Albrecht Ott¹

¹Department of Experimental Physics, Saarland University, ²Institute of Chemistry, Technische Universität Berlin, ³School of Physics and Astronomy, University of Minnesota

JoVE 54273

FRET Imaging in Three-dimensional Hydrogels

Amalie E. Donius*¹, Sylvain V. Bougoin*^1,3, Juan M. Taboas^1,2

¹Department of Oral Biology, Center for Craniofacial Regeneration, McGowan Institute of Regenerative Medicine, University of Pittsburgh, ²Department of Bioengineering, Center for Craniofacial Regeneration, McGowan Institute of Regenerative Medicine, University of Pittsburgh, ³Laerdal AS

JoVE 54135

Assessment of Myofilament Ca²⁺ Sensitivity Underlying Cardiac Excitation-contraction Coupling

Zai Hao Zhao¹, Chun Li Jin¹, Ji Hyun Jang¹, Yu Na Wu¹, Sung Joon Kim¹, Hong Hua Jin², Lan Cui², Yin Hua Zhang^1,2,3

¹Department of Physiology & Biomedical Sciences, Ischemic/hypoxic Disease Institute, Seoul National University College of Medicine, ²Yan Bian University Hospital, ³Institute of Cardiovascular Sciences, University of Manchester

JoVE 54057

Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method

Clarisse van der Feltz¹, Daniel Pomeranz Krummel^1,2

¹Department of Biochemistry, Brandeis University, ²Winship Cancer Institute, Emory University School of Medicine

JoVE 54389

Genetic and Biochemical Approaches for In Vivo and In Vitro Assessment of Protein Oligomerization: The Ryanodine Receptor Case Study

Paulina J. Stanczyk¹, F. Anthony Lai¹, Spyros Zissimopoulos¹

¹Wales Heart Research Institute, Cardiff University School of Medicine, College of Biomedical and Life Sciences

JoVE 54271

Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density Lipoprotein

Mulugeta Seneshaw¹, Faridoddin Mirshahi¹, Hae-Ki Min¹, Amon Asgharpour¹, Shervin Mirshahi¹, Kalyani Daita¹, Sherry Boyett¹, Prasanna K. Santhekadur², Michael Fuchs¹, Arun J. Sanyal¹

¹Division of Gastroenterology, Hepatology and Nutrition, Virginia Commonwealth University, ²McGuire Veterans Affairs Medical Center, Research BC-101

JoVE 54257

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Qianfan Bai*¹, Zhiqiang Bai*¹, Shaohai Xu³, Lei Sun^1,2

¹Cardiovascular and Metabolic Disorders Program, Duke-NUS Medical School, ²Institute of Molecular and Cell Biology, Singapore, ³Division of Bioengineering, School of Chemical & Biomedical Engineering, Nanyang Technological University

JoVE 54207

Optimal Lentivirus Production and Cell Culture Conditions Necessary to Successfully Transduce Primary Human Bronchial Epithelial Cells

Nathalie Baumlin-Schmid¹, Matthias Salathe¹, Nevis L. Fregien²

¹Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, University of Miami, Miller School of Medicine, ²Department of Cell Biology, University of Miami, Miller School of Medicine

JoVE 54176

Measurement of Survival Time in Brachionus Rotifers: Synchronization of Maternal Conditions

Gen Kaneko^1,4, Tatsuki Yoshinaga², Kristin E. Gribble³, David M. Welch³, Hideki Ushio¹

¹Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, ²School of Marine Biosciences, Department of Marine Biosciences, Kitasato University, ³Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, ⁴School of Arts and Sciences, University of Houston-Victoria

JoVE 54126

Development of an Insert Co-culture System of Two Cellular Types in the Absence of Cell-Cell Contact

Justine Renaud¹, Maria-Grazia Martinoli¹

¹Dept. of Medical Biology, University of Québec

JoVE 54356

Implementation of a Coherent Anti-Stokes Raman Scattering (CARS) System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope

Vasyl Mytskaniuk*¹, Fabrice Bardin*^2,3, Hassan Boukhaddaoui^1,5, Herve Rigneault⁴, Nicolas Tricaud¹

¹INSERM U1051, Institut des Neurosciences de Montpellier (INM), Université de Montpellier, ²Université de Nîmes, ³CNRS, IES, UMR 5214, ⁴Aix-Marseille Université, CNRS, École Centrale Marseille, Institut Fresnel, UMR 7249, ⁵Montpellier RIO Imaging (MRI)

JoVE 54262

Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury

Robert A. McKee^1,2, Rebecca A. Wingert^1,2

¹Center for Zebrafish Research, Department of Biological Sciences, University of Notre Dame, ²Center for Stem Cells and Regenerative Medicine, Department of Biological Sciences, University of Notre Dame

JoVE 54241

Hydroponics: A Versatile System to Study Nutrient Allocation and Plant Responses to Nutrient Availability and Exposure to Toxic Elements

Nga T. Nguyen¹, Samuel A. McInturf¹, David G. Mendoza-Cózatl¹

¹Division of Plant Sciences, C.S. Bond Life Sciences Center, University of Missouri, Columbia

JoVE 54317

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses

Agnieszka B. Bialkowska*¹, Amr M. Ghaleb*¹, Mandayam O. Nandan¹, Vincent W. Yang^1,2

¹Department of Medicine, Stony Brook University School of Medicine, ²Department of Physiology & Biophysics, Stony Brook University School of Medicine

JoVE 54161

Development of a Colloidal Gold-based Immunochromatographic Test Strip for Detection of Cetacean Myoglobin

Kun-Wei Chan¹, Chieh Lo¹, Chi-Shih Chu², Li-Te Chin², Yu-Ting Wang¹, Wei-Cheng Yang¹

¹Department of Veterinary Medicine, National Chiayi University, ²Department of Microbiology and Immunology/Graduate Institute of Biomedical and Biopharmaceutical Sciences, National Chiayi University

JoVE 53433

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Stefanie Arndt*¹, Heidi-Kristin Walter*¹, Hans-Achim Wagenknecht¹

¹Institute of Organic Chemistry, Karlsruhe Institute of Technology

JoVE 54121

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

Michael Allen Stiffler¹, Subu K Subramanian¹, Victor H Salinas¹, Rama Ranganathan¹

¹Green Center for Systems Biology, University of Texas Southwestern Medical Center

JoVE 54119

Removal of Exogenous Materials from the Outer Portion of Frozen Cores to Investigate the Ancient Biological Communities Harbored Inside

Robyn A. Barbato¹, Natàlia Garcia-Reyero², Karen Foley¹, Robert Jones¹, Zoe Courville³, Thomas Douglas⁴, Edward Perkins², Charles M. Reynolds⁴

¹Biogeochemical Sciences Branch, Cold Regions Research and Engineering Laboratory, US Army Engineer Research & Development Center, Hanover, NH, ²Environmental Processes Branch, Environmental Laboratory, US Army Engineer Research & Development Center, Vicksburg, MS, ³Terrestrial and Cryospheric Scienes Branch, Cold Regions Research and Engineering Laboratory, US Army Engineer Research & Development Center, Hanover, NH, ⁴Biogeochemical Sciences Branch, Cold Regions Research and Engineering Laboratory, US Army Engineer Research & Development Center, Fairbanks, AK

JoVE 54091

Isolation of Mouse Coronary Endothelial Cells

Shizhen Luo¹, Aaron H. Truong¹, Ayako Makino¹

¹Department of Physiology, University of Arizona, Tucson

JoVE 53985

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Cancer Research

Developmental Biology

Immunology and Infection

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