JoVE Journal

Biology

JoVE Biology includes techniques and experimental approaches in the fields of cellular, molecular, and organismal biology.

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Isolation of Intact, Whole Mouse Mammary Glands for Analysis of Extracellular Matrix Expression and Gland Morphology

Christopher Thompson¹, Katherine Keck², Abigail Hielscher²

¹Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, ²Department of Biomedical Sciences, GA-PCOM

The Murine Choline-Deficient, Ethionine-Supplemented (CDE) Diet Model of Chronic Liver Injury

Jully Gogoi-Tiwari¹, Julia Köhn-Gaone¹, Corey Giles², Dirk Schmidt-Arras³, Francis D. Gratte^1,4, Caryn L. Elsegood¹, Geoffrey W. McCaughan^5,6,7, Grant A. Ramm^8,9, John K. Olynyk^10,11, Janina E.E. Tirnitz-Parker^1,12

¹School of Biomedical Sciences & Curtin Health Innovation Research Institute, Curtin University, ²School of Public Health & Curtin Health Innovation Research Institute, Curtin University, ³Institute of Biochemistry, Christian-Albrechts-University, ⁴School of Veterinary and Life Sciences, Murdoch University, ⁵Centenary Institute of Cancer Medicine and Cell Biology, The University of Sydney, ⁶Royal Prince Alfred Hospital, ⁷A.W. Morrow Gastroenterology and Liver Centre, ⁸QIMR Berghofer Medical Research Institute, ⁹Faculty of Medicine and Biomedical Sciences, The University of Queensland, ¹⁰Fiona Stanley and Fremantle Hospitals, ¹¹School of Medical and Health Sciences, Edith Cowan University, ¹²School of Medicine and Pharmacology, University of Western Australia

Standardized Method for High-throughput Sterilization of Arabidopsis Seeds

Benson E. Lindsey III*¹, Luz Rivero*¹, Chistopher S. Calhoun¹, Erich Grotewold^1,2, Jelena Brkljacic¹

¹Arabidopsis Biological Resource Center, Center for Applied Plant Sciences, The Ohio State University, ²Department of Molecular Genetics, Center for Applied Plant Sciences, The Ohio State University

Assessing Urinary Tract Junction Obstruction Defects by Methylene Blue Dye Injection

¹Cancer and Developmental Biology Laboratory, National Cancer Institute, National Institutes of Health

3' End Sequencing Library Preparation with A-seq2

Georges Martin¹, Ralf Schmidt¹, Andreas J. Gruber¹, Souvik Ghosh¹, Walter Keller¹, Mihaela Zavolan^1,2

¹Computational and Systems Biology, Biozentrum, University of Basel, ²Swiss Institute of Bioinformatics, Biozentrum, University of Basel

Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers

Meike Koester^1,2, René Gergs^1,3

¹Institute for Environmental Sciences, University of Koblenz-Landau, ²Institute of Integrated Natural Sciences, University of Koblenz-Landau, ³IV 2.5 (Trace Analysis, Artificial Pond and stream system), Federal Environment Agency

Subcellular Fractionation for ERK Activation Upon Mitochondrial-derived Peptide Treatment

Su-Jeong Kim¹, Jialin Xiao¹, Pinchas Cohen¹, Kelvin Yen¹

¹Leonard Davis School of Gerontology, University of Southern California

Precise, High-throughput Analysis of Bacterial Growth

Masaomi Kurokawa¹, Bei-Wen Ying^1,2

¹Graduate School of Life and Environmental Sciences, University of Tsukuba, ²Institute of Biology and Information Science, East China Normal University

Confocal Imaging of Neuropeptide Y-pHluorin: A Technique to Visualize Insulin Granule Exocytosis in Intact Murine and Human Islets

Madina Makhmutova¹, Tao Liang², Herbert Gaisano², Alejandro Caicedo¹, Joana Almaça¹

¹Department of Medicine, University of Miami, ²Department of Medicine, University of Toronto

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

Gail M Nelson¹, Jenna M Guynn², Brian N Chorley¹

¹National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, ²Oak Ridge Institute for Science and Education at U.S. Environmental Protection Agency

Study of Protein-protein Interactions in Autophagy Research

Secil Erbil-Bilir¹, Nur M. Kocaturk¹, Melih Yayli², Devrim Gozuacik^1,3

¹Molecular Biology, Genetics and Bioengineering Program, Sabanci University, ²Information Center, Sabanci University, ³EFSUN, Center of Excellence for Functional Surfaces and Interfaces for Nano Diagnostics, Sabanci University

An In Vitro Model for Studying Cellular Transformation by Kaposi Sarcoma Herpesvirus

Shane C. McAllister¹, Ryan S. Hanson¹, Kyleen N. Grissom¹, Sara Botto², Ashlee V. Moses²

¹Division of Pediatric Infectious Diseases, University of Minnesota Medical School, ²Vaccine and Gene Therapy Institute, Oregon Health and Science University

Volume Segmentation and Analysis of Biological Materials Using SuRVoS (Super-region Volume Segmentation) Workbench

Michele C. Darrow¹, Imanol Luengo^1,2, Mark Basham¹, Matthew C. Spink¹, Sarah Irvine¹, Andrew P. French², Alun W. Ashton¹, Elizabeth M.H. Duke¹

¹Science Division, Harwell Science and Innovation Campus, Diamond Light Source, ²School of Computer Science, University of Nottingham

Fat Body Organ Culture System in Aedes Aegypti, a Vector of Zika Virus

Hae-Na Chung¹, Stacy D. Rodriguez¹, Victoria K. Carpenter², Julia Vulcan¹, C. Donovan Bailey¹, Madhugiri Nageswara-Rao¹, Yiyi Li³, Geoffrey M. Attardo⁴, Immo A. Hansen^1,5

¹Department of Biology, New Mexico State University, ²Department of Molecular Genetics and Microbiology, Duke University, ³Department of Computer Sciences, New Mexico State University, ⁴Department of Epidemiology of Microbial Diseases, Yale School of Public Health, ⁵Institute of Applied Biosciences, New Mexico State University

A Protocol for Using Gene Set Enrichment Analysis to Identify the Appropriate Animal Model for Translational Research

Christopher Weidner¹, Matthias Steinfath¹, Elisa Wistorf¹, Michael Oelgeschläger¹, Marlon R. Schneider¹, Gilbert Schönfelder^1,2

¹Department of Experimental Toxicology and ZEBET, German Federal Institute for Risk Assessment (BfR), ²Department of Clinical Pharmacology and Toxicology, Charité-Universitätsmedizin Berlin

Real-time In Vivo Recording of Arabidopsis Calcium Signals During Insect Feeding Using a Fluorescent Biosensor

Thomas R. Vincent¹, James Canham¹, Masatsugu Toyota^2,3,4, Marieta Avramova¹, Sam T. Mugford⁵, Simon Gilroy², Anthony J. Miller¹, Saskia Hogenhout⁵, Dale Sanders¹

¹Department of Metabolic Biology, John Innes Centre, Norwich Research Park, ²Department of Botany, University of Wisconsin, Madison, ³Department of Biochemistry and Molecular Biology, Saitama University, ⁴Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST), ⁵Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park

Maintaining Aedes aegypti Mosquitoes Infected with Wolbachia

Perran A. Ross¹, Jason K. Axford¹, Kelly M. Richardson¹, Nancy M. Endersby-Harshman¹, Ary A. Hoffmann¹

¹School of BioSciences, Bio21 Institute and University of Melbourne

Optical Quantification of Intracellular pH in Drosophila melanogaster Malpighian Tubule Epithelia with a Fluorescent Genetically-encoded pH Indicator

Adam J. Rossano¹, Michael F. Romero^1,2

¹Department of Physiology and Biomedical Engineering, Mayo Clinic College of Medicine, ²Department of Nephrology and Hypertension, Mayo Clinic College of Medicine

Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass

Hieng Chiong Tie¹, Bing Chen¹, Xiuping Sun¹, Li Cheng^2,3, Lei Lu¹

¹School of Biological Sciences, Nanyang Technological University, ²Bioinformatics Institute, ³School of Computing, National University of Singapore

High-throughput Screening for Protein-based Inheritance in S. cerevisiae

James S. Byers¹, Daniel F. Jarosz^1,2

¹Department of Developmental Biology, Stanford University School of Medicine, ²Department of Chemical and Systems Biology, Stanford University School of Medicine

A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice

Chrstine Bäuerl*¹, Özgun C.O. Umu*², Pablo E. Hernandez³, Dzung B. Diep⁴, Gaspar Pérez-Martínez¹

¹Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), ²Department of Food Safety and Infection Biology, Norwegian University of Life Sciences (NMBU), ³Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid (UCM), ⁴Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU)

A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling

Naeem Nathoo^1,2, Mark A. Bernards², Jacqueline MacDonald³, Ze-Chun Yuan^1,3

¹London Research and Development Centre, Agriculture & Agri-Food Canada, ²Department of Biology, University of Western Ontario, ³Department of Microbiology and Immunology, University of Western Ontario

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry

Haley R. Pugsley¹

¹Amnis Biology Department, MilliporeSigma

Determination of Sialic Acids in Liver and Milk Samples of Wild-type and CMAH Knock-out Mice.

Cui Cao¹, Wen J. Wang¹, Ying Y. Huang¹, Hong L. Yao¹, Louis P. Conway¹, Li Liu¹, Josef Voglmeir¹

¹Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University

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