JoVE Journal

Biology

JoVE Biology includes techniques and experimental approaches in the fields of cellular, molecular, and organismal biology.

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Environmental Screening of Aeromonas hydrophila, Mycobacterium spp., and Pseudocapillaria tomentosa in Zebrafish Systems

Jean-Philippe Mocho¹, Darren J. Martin¹, Mollie E. Millington¹, Yolanda Saavedra Torres¹

¹Biological Research Facility, The Francis Crick Institute

Pulling Membrane Nanotubes from Giant Unilamellar Vesicles

Coline Prévost*^1,2,3, Feng-Ching Tsai*^1,4, Patricia Bassereau^1,4, Mijo Simunovic^1,5

¹Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS UMR168, ²Department of Genetics and Complex Diseases, T. H. Chan School of Public Health, Harvard Medical School, ³Department of Cell Biology, Harvard Medical School, ⁴Sorbonne Universités, UPMC University Paris 06, ⁵Center for Studies in Physics and Biology, The Rockefeller University

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Mohammed A. Amin¹, Dileep Varma¹

¹Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University

Investigating the Detrimental Effects of Low Pressure Plasma Sterilization on the Survival of Bacillus subtilis Spores Using Live Cell Microscopy

Felix M. Fuchs¹, Marina Raguse^1,2,3, Marcel Fiebrandt², Kazimierz Madela⁴, Peter Awakowicz², Michael Laue⁴, Katharina Stapelmann³, Ralf Moeller¹

¹Department of Radiation Biology, Institute of Aerospace Medicine, Space Microbiology Research Group, German Aerospace Center (DLR e.V.), ²Institute of Electrical Engineering and Plasma Technology, Faculty of Electrical Engineering and Information Technology, Ruhr-University Bochum, ³Institute of Electrical Engineering and Plasma Technology, Faculty of Electrical Engineering and Information Technology, Biomedical Applications of Plasma Technology, Ruhr-University Bochum, ⁴Advanced Light and Electron Microscopy (ZBS 4), Robert Koch Institute

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Caroline Haupt*¹, Tommy Hofmann*¹, Sabine Wittig*¹, Susann Kostmann¹, Argyris Politis², Carla Schmidt¹

¹Interdisciplinary research center HALOmem, Martin Luther University Halle-Wittenberg, ²Department of Chemistry, Kings College London

Methods to Study Changes in Inherent Protein Aggregation with Age in Caenorhabditis elegans

Nicole Groh^1,2, Ivan Gallotta¹, Marie C. Lechler^1,2, Chaolie Huang¹, Raimund Jung¹, Della C. David¹

¹Protein Aggregation and Aging, German Center for Neurodegenerative Diseases (DZNE), ²Graduate School of Cellular and Molecular Neuroscience, German Center for Neurodegenerative Diseases (DZNE)

Hemi-laryngeal Setup for Studying Vocal Fold Vibration in Three Dimensions

Christian T. Herbst^1,2, Vit Hampala¹, Maxime Garcia^2,3, Riccardo Hofer², Jan G. Svec¹

¹Voice Research Lab, Department of Biophysics, Faculty of Science, Palacky University Olomouc, ²Laboratory of Bio-Acoustics, Dept. of Cognitive Biology, University of Vienna, ³ENES Lab, NEURO-PSI,CNRS UMR 9197, Université Lyon/Saint-Etienne, France

Live Cell Fluorescence Microscopy to Observe Essential Processes During Microbial Cell Growth

Matthew Howell¹, Jeremy J. Daniel¹, Pamela J.B. Brown¹

¹Division of Biological Sciences, University of Missouri

Preparation of Meiotic Chromosome Spreads from Mouse Spermatocytes

Ferdusy Dia¹, Tierra Strange¹, Jenny Liang¹, Jacob Hamilton¹, Karen M. Berkowitz^1,2

¹Department of Biochemistry & Molecular Biology, Drexel University College of Medicine, ²Department of Obstetrics & Gynecology, Drexel University College of Medicine

Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics

Kaiwen Sun*¹, Yuyu Zheng*¹, Ziqiang Zhu¹

¹Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University

Establishment of an Extracellular Acidic pH Culture System

Ayano Kondo¹, Tsuyoshi Osawa²

¹Innovative Technology Laboratories, Kyowa Hakko Kirin Co., Ltd, ²Laboratory for Systems Biology and Medicine, RCAST, The University of Tokyo

Monitoring Colony-level Effects of Sublethal Pesticide Exposure on Honey Bees

William G. Meikle¹, Milagra Weiss¹

¹Carl Hayden Bee Research Center

Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA

Katrin Kreisel¹, Martin K.M. Engqvist^1,2, Anders R. Clausen¹

¹Department for Medical Biochemistry and Cell Biology, University of Gothenburg, ²Department of Biology and Biological Engineering, Chalmers University of Technology

Endotracheal Intubation of Rabbits Using a Polypropylene Guide Catheter

Krista L Thompson¹, Thomas R Meier¹, Jodi A Scholz¹

¹Department of Comparative Medicine, Mayo Clinic

Advanced Confocal Microscopy Techniques to Study Protein-protein Interactions and Kinetics at DNA Lesions

Soňa Legartová*¹, Jana Suchánková*¹, Jana Krejčí¹, Alena Kovaříková¹, Eva Bártová¹

¹Institute of Biophysics of the Czech Academy of Sciences

Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation

Yulia Artemenko¹, Peter N. Devreotes²

¹Department of Biological Sciences, SUNY Oswego, ²Department of Cell Biology, Johns Hopkins University School of Medicine

A Mouse 5/6^th Nephrectomy Model That Induces Experimental Uremic Cardiomyopathy

Xiaoliang Wang*¹, Muhammad A. Chaudhry*², Ying Nie², Zijian Xie¹, Joseph I. Shapiro², Jiang Liu^1,2

¹Marshall Institute for Interdisciplinary Research (MIIR), Marshall University, ²Department of Biomedical Sciences, Marshall University Joan C. Edwards School of Medicine

Ground State Depletion Super-resolution Imaging in Mammalian Cells

Rose E. Dixon¹, Oscar Vivas¹, Karen I. Hannigan¹, Eamonn J. Dickson¹

¹Department of Physiology and Membrane Biology, University of California School of Medicine, Davis

In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells

Fengxi Meng*^1,2, Xiaofeng Li*^1,2, Hui Ren^1,2, Jiang Qian^1,2

¹Department of Ophthalmology, Eye and ENT Hospital of Fudan University, ²Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University

Isolation of Intact, Whole Mouse Mammary Glands for Analysis of Extracellular Matrix Expression and Gland Morphology

Christopher Thompson¹, Katherine Keck², Abigail Hielscher²

¹Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, ²Department of Biomedical Sciences, GA-PCOM

The Murine Choline-Deficient, Ethionine-Supplemented (CDE) Diet Model of Chronic Liver Injury

Jully Gogoi-Tiwari¹, Julia Köhn-Gaone¹, Corey Giles², Dirk Schmidt-Arras³, Francis D. Gratte^1,4, Caryn L. Elsegood¹, Geoffrey W. McCaughan^5,6,7, Grant A. Ramm^8,9, John K. Olynyk^10,11, Janina E.E. Tirnitz-Parker^1,12

¹School of Biomedical Sciences & Curtin Health Innovation Research Institute, Curtin University, ²School of Public Health & Curtin Health Innovation Research Institute, Curtin University, ³Institute of Biochemistry, Christian-Albrechts-University, ⁴School of Veterinary and Life Sciences, Murdoch University, ⁵Centenary Institute of Cancer Medicine and Cell Biology, The University of Sydney, ⁶Royal Prince Alfred Hospital, ⁷A.W. Morrow Gastroenterology and Liver Centre, ⁸QIMR Berghofer Medical Research Institute, ⁹Faculty of Medicine and Biomedical Sciences, The University of Queensland, ¹⁰Fiona Stanley and Fremantle Hospitals, ¹¹School of Medical and Health Sciences, Edith Cowan University, ¹²School of Medicine and Pharmacology, University of Western Australia

Standardized Method for High-throughput Sterilization of Arabidopsis Seeds

Benson E. Lindsey III*¹, Luz Rivero*¹, Chistopher S. Calhoun¹, Erich Grotewold^1,2, Jelena Brkljacic¹

¹Arabidopsis Biological Resource Center, Center for Applied Plant Sciences, The Ohio State University, ²Department of Molecular Genetics, Center for Applied Plant Sciences, The Ohio State University

Assessing Urinary Tract Junction Obstruction Defects by Methylene Blue Dye Injection

¹Cancer and Developmental Biology Laboratory, National Cancer Institute, National Institutes of Health

3' End Sequencing Library Preparation with A-seq2

Georges Martin¹, Ralf Schmidt¹, Andreas J. Gruber¹, Souvik Ghosh¹, Walter Keller¹, Mihaela Zavolan^1,2

¹Computational and Systems Biology, Biozentrum, University of Basel, ²Swiss Institute of Bioinformatics, Biozentrum, University of Basel

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