JoVE Journal

Biology

JoVE Biology publishes standard techniques and novel experimental approaches in the fields of cellular, molecular, and organismal biology aimed at understanding the functions of life and living organisms.

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Time-Lapse Video Microscopy for Assessment of EYFP-Parkin Aggregation as a Marker for Cellular Mitophagy

Gabriele Di Sante¹, Mathew C. Casimiro¹, Timothy G. Pestell², Richard G. Pestell^1,2,3

¹Department of Cancer Biology, Thomas Jefferson University, SKCC, ²Department of Medical Oncology, Thomas Jefferson University, SKCC, ³Kazan Federal University

JoVE 53657

Sequential Application of Glass Coverslips to Assess the Compressive Stiffness of the Mouse Lens: Strain and Morphometric Analyses

Catherine Cheng¹, David S. Gokhin¹, Roberta B. Nowak¹, Velia M. Fowler¹

¹Department of Cell and Molecular Biology, The Scripps Research Institute

JoVE 53986

Measuring Pressure Volume Loops in the Mouse

DeWayne Townsend¹

¹Department of Integrative Biology and Physiology, University of Minnesota

JoVE 53810

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

Thomas B. Jacobs¹, Gregory B. Martin^1,2

¹Boyce Thompson Institute for Plant Research, ²Section of Plant Pathology & Plant-Microbe Biology, School of Integrative Plant Science, Cornell University

JoVE 53843

Rapid Verification of Terminators Using the pGR-Blue Plasmid and Golden Gate Assembly

Jace C. Bradshaw¹, Allea Belle Gongola¹, Nathan S. Reyna¹

¹Department of Biology, Ouachita Baptist University

JoVE 54064

The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo

Anne-Sophie Perrin-Terrin^1,2, Florine Jeton^1,3, Aurelien Pichon^1,3,4, Alain Frugière², Jean-Paul Richalet^1,3, Laurence Bodineau², Nicolas Voituron^1,3

¹Sorbonne Paris Cité, Laboratory “Hypoxia & Lung” EA2363, University Paris 13, ²UPMC Univ Paris 06, INSERM, UMR_S1158 Neurophysiologie Respiratoire Expérimentale et Clinique, Sorbonne Universités, ³Laboratory of Excellence GR-Ex, ⁴Laboratory MOVE (EA 6314), University of Poitiers

JoVE 53613

Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids

Kelly Yarnell¹, Rebecca S. Purcell², Susan L. Walker²

¹School of Animal Rural and Environmental Science, Nottingham Trent University, ²Chester Zoo, Upton-by-Chester

JoVE 53479

Analysis of LINE-1 Retrotransposition at the Single Nucleus Level

Pasano Bojang¹, Kenneth S. Ramos^1,2

¹Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, University of Arizona College of Medicine, ²Center for Applied Genetics and Genomic Medicine, University of Arizona College of Medicine

JoVE 53753

Production of Human Norovirus Protruding Domains in E. coli for X-ray Crystallography

Mila M. Leuthold^1,2, Anna D. Koromyslova^1,2, Bishal K. Singh^1,2, Grant S. Hansman^1,2

¹Schaller Research Group, University of Heidelberg and the German Cancer Research Center, ²Department of Infectious Diseases, Virology, University of Heidelberg

JoVE 53845

Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae

Bryce W. Buchanan¹, Michael E. Lloyd^1,2, Sarah M. Engle¹, Eric M. Rubenstein¹

¹Department of Biology, Ball State University, ²Bioproduct Research & Development, Eli Lilly and Company

JoVE 53975

A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response

Ritesh Kumar*¹, Shu Wei Wu*¹, Arya Bagus Boedi Iswanto¹, Dhinesh Kumar¹, Xiao Han², Jae-Yean Kim¹

¹Division of Applied Life Science (BK21 plus program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, ²Biotechnology Research Institute, Chinese Academy of Agricultural Sciences

JoVE 53513

Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization

Melis N. Anahtar¹, Brittany A. Bowman¹, Douglas S. Kwon¹

¹Ragon Institute of MGH, MIT, and Harvard, Massachusetts General Hospital

JoVE 53939

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Broder Rühmann¹, Jochen Schmid¹, Volker Sieber¹

¹Chair of Chemistry of Biogenic Resources, Technische Universität München

JoVE 53249

Procedure to Evaluate the Efficiency of Flocculants for the Removal of Dispersed Particles from Plant Extracts

Johannes F. Buyel^1,2

¹Institute for Molecular Biology and Applied Ecology IME, Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e. V., ²Institute for Molecular Biotechnology, RWTH Aachen University

JoVE 53940

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Tyler M. Bauman^1,2, Emily A. Ricke², Sally A. Drew³, Wei Huang^3,4, William A. Ricke^2,4

¹Division of Urologic Surgery, Washington University in St. Louis School of Medicine, ²Department of Urology, University of Wisconsin School of Medicine and Public Health, ³Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, ⁴O’Brien Urology Research Center, University of Wisconsin School of Medicine and Public Health

JoVE 53837

Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells

Henrik D. Møller¹, Rasmus K. Bojsen², Chris Tachibana³, Lance Parsons⁴, David Botstein⁵, Birgitte Regenberg¹

¹Department of Biology, University of Copenhagen, ²National Veterinary Institute, Technical University of Denmark, ³Group Health Research Institute, ⁴Lewis-Sigler Institute for Integrative Genomics, Princeton University, ⁵Calico Life Sciences LLC

JoVE 54239

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Javier Viña-Gonzalez¹, David Gonzalez-Perez¹, Miguel Alcalde¹

¹Department of Biocatalysis, Institute of Catalysis, CSIC

JoVE 53761

Protocol for Isolation of Primary Human Hepatocytes and Corresponding Major Populations of Non-parenchymal Liver Cells

Victoria Kegel*¹, Daniela Deharde*¹, Elisa Pfeiffer¹, Katrin Zeilinger², Daniel Seehofer¹, Georg Damm¹

¹Department of General-, Visceral- and Transplantation Surgery, Charité University Medicine Berlin, ²Brandenburg Center for Regenerative Therapies, Charité University Medicine Berlin

JoVE 53069

Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites

Erik Serrao¹, Peter Cherepanov², Alan N. Engelman¹

¹Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, ²Chromatin Structure and Mobile DNA, The Francis Crick Institute

JoVE 53840

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System

Ying Zhang¹, Juan Dong^1,2

¹The Waksman Institute of Microbiology, Rutgers, The State University of New Jersey, ²The Department of Plant Biology and Pathology, Rutgers, The State University of New Jersey