JoVE Journal

Biology

JoVE Biology includes techniques and experimental approaches in the fields of cellular, molecular, and organismal biology.

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Continuous-wave Thulium Laser for Heating Cultured Cells to Investigate Cellular Thermal Effects

Yoko Miura^1,2,3, Joachim Pruessner¹, Carla Lotta Mertineit¹, Katharina Kern¹, Michael Muenter¹, Moritz Moltmann², Veit Danicke², Ralf Brinkmann^1,2

¹Institute of Biomedical Optics, University of Luebeck, ²Medical Laser Center Luebeck GmbH, University of Leubeck, ³Department of Ophthalmology, University of Luebeck

Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes

Stephanie Chanon¹, Christine Durand¹, Aurelie Vieille-Marchiset¹, Maud Robert², Charna Dibner³, Chantal Simon¹, Etienne Lefai¹

¹CarMeN Laboratory, INSERM U1060, INRA 1397, University of Lyon, ²Department of digestive and bariatric surgery, Obesity Integrated Center, University Hospital of Edouard Herriot, Hospices Civils de Lyon, Lyon 1 University, ³Division of Endocrinology, Diabetes, Hypertension and Nutrition, Department of Clinical Medicine, Faculty of Medicine, University of Geneva

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

Orlando Santillán¹, Miguel A. Ramírez-Romero², Guillermo Dávila³

¹Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, ²Oncomedic Incorporation, Ciudad de México, ³Laboratorio Internacional de Investigación sobre el Genoma Humano, Universidad Nacional Autónoma de México

Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy

Heiko Lemcke^1,2,3, Natalia Voronina^1,2,3, Gustav Steinhoff^1,2,3, Robert David^1,2,3

¹Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), ²Department of Cardiac Surgery, University of Rostock, ³Department of Life, Light and Matter of the Interdisciplinary Faculty, University of Rostock

Clock Scan Protocol for Image Analysis: ImageJ Plugins

Maxim Dobretsov¹, Georg Petkau², Abdallah Hayar³, Eugen Petkau¹

¹Department of Anesthesiology, University of Arkansas for Medical Sciences, ²Department of Lymphocyte Development, Max Plank Institute for Infection Biology, ³Department of Neurobiology & Developmental Sciences, University of Arkansas for Medical Sciences

Methods for the Extraction of Endosymbionts from the Whitefly Bemisia tabaci

Dan-Tong Zhu¹, Xin-Ru Wang¹, Fei-Xue Ban¹, Chi Zou¹, Shu-Sheng Liu¹, Xiao-Wei Wang¹

¹Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogen and Insects, Institute of Insect Sciences, Zhejiang University

DiI Perfusion as a Method for Vascular Visualization in Ambystoma mexicanum

Anna J. Saltman*^1,2, May Barakat*¹, Donald M. Bryant¹, Anastasia Brodovskaya¹, Jessica L. Whited¹

¹Department of Orthopedic Surgery, Harvard Medical School and Brigham and Women's Hospital, ²Division of Graduate Medical Sciences, Boston University

Using a Whole-mount Immunohistochemical Method to Study the Innervation of the Biliary Tract in Suncus murinus

Ke Ren¹, Yidan Dai¹, Kai Yi², Masanobu Kinoshita¹, Masahiro Itoh², Ichiro Sakata³, Takafumi Sakai³, Shuang-Qin Yi¹

¹Department of Frontier Health Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, ²Department of Anatomy, Tokyo Medical University, ³Area of Regulatory Biology, Division of Life Science, Graduate School of Science and Engineering, Saitama University

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells

Francesco Castagnetti^1,2, Elisabetta Fiacco³, Carol Imbriano⁴, Lucia Latella^1,2

¹Department of Medicine, Institute of Translational Pharmacology, Italian National Research Council, ²Epigenetics and Regenerative Medicine, IRCCS Fondazione Santa Lucia, ³Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), ⁴Department of Life Sciences, University of Modena and Reggio Emilia

Murine Aortic Crush Injury: An Efficient In Vivo Model of Smooth Muscle Cell Proliferation and Endothelial Function

Dan Yu^1,4, George Makkar², Rajabrata Sarkar^2,3,4, Dudley K. Strickland^2,3,4, Thomas S. Monahan^1,2,4

¹Department of Surgery, Baltimore Veterans Affairs Medical Center, ²Department of Surgery, University of Maryland School of Medicine, ³Department of Physiology, University of Maryland School of Medicine, ⁴Center for Vascular and Inflammatory Diseases, University of Maryland School of Medicine

Monitoring ER/SR Calcium Release with the Targeted Ca²⁺ Sensor CatchER⁺

Florence N. Reddish¹, Cassandra L. Miller¹, Rakshya Gorkhali¹, Jenny J. Yang¹

¹Department of Chemistry, Center of Diagnostics and Therapeutics (CDT), Georgia State University

Simultaneous Isolation of High Quality Cardiomyocytes, Endothelial Cells, and Fibroblasts from an Adult Rat Heart

Dursun Gündüz¹, Christian W. Hamm¹, Muhammad Aslam¹

¹Internal Medicine, Cardiology and Angiology, University Hospital Giessen

En Face Preparation of Mouse Blood Vessels

Kyung Ae Ko¹, Keigi Fujiwara¹, Sunil Krishnan², Jun-ichi Abe¹

¹Department of Cardiology, Division of Internal Medicine, University of Texas MD Anderson Cancer Center, ²Department of Radiation Oncology, University of Texas MD Anderson Cancer Center

Cellular Redox Profiling Using High-content Microscopy

Tom Sieprath^1,2, Tobias Corne^1,2, Joke Robijns¹, Werner J. H. Koopman³, Winnok H. De Vos^1,2

¹Laboratory of Cell Biology and Histology, Department of Veterinary Sciences, University of Antwerp, ²Cell Systems and Imaging Research Group (CSI), Department of Molecular Biotechnology, Ghent University, ³Department of Biochemistry , Radboud Institute for Molecular Life Sciences, Radboud University Medical Center

Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules

Anaïs Aulas*^1,2, Marta M. Fay*^1,2, Witold Szaflarski^1,2,3, Nancy Kedersha^1,2, Paul Anderson^1,2, Pavel Ivanov^1,2,4

¹Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital, ²Department of Medicine, Harvard Medical School, ³Department of Histology and Embryology, Poznan University of Medical Sciences, ⁴The Broad Institute of Harvard and M.I.T.

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Sandrine Schmutz¹, Mariana Valente^2,3,4,5, Ana Cumano², Sophie Novault¹

¹Flow Cytometry Core Facility, Center for Translational Research-Technical Core, Institut Pasteur, ²Unit for Lymphopoiesis, Immunology Department, INSERM U1223, University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Institut Pasteur, ³Stem-Cell Microenvironments in Repair/Regeneration Team, Instituto de Investigação e Inovação em Saúde (i3s), INEB - Instituto de Engenharia Biomédica, ⁴ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, ⁵Stem Cells and Regenerative Medicine Team, UMRS 1166, ICAN - Institute of Cardiometabolism And Nutrition, UPMC - Université Pierre et Marie Curie - Paris 6, INSERM

Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy

Corinne Blancard¹, Bénédicte Salin¹

¹Institut de Biochimie et Génétique Cellulaires, Centre National de la Recherche Scientifique, UMR 5095, Université de Bordeaux

Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy

Shuo Li*^1,2, Sumana Raychaudhuri*¹, Shigeki Watanabe^1,3

¹Department of Cell Biology, Johns Hopkins School of Medicine, ²Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, ³Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine

Techniques to Induce and Quantify Cellular Senescence

Nicole Noren Hooten¹, Michele K. Evans¹

¹Laboratory of Epidemiology and Population Science, National Institute on Aging, National Institutes of Health

Sample Preparation for Mass Cytometry Analysis

Ryan L. McCarthy¹, Aundrietta D. Duncan¹, Michelle C. Barton¹

¹Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center

Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells

Jeffrey A. Fabrick¹, J. Joe Hull¹

¹U.S. Arid Land Agricultural Research Center, Agricultural Research Service, U.S. Department of Agriculture

Mixed Primary Cultures of Murine Small Intestine Intended for the Study of Gut Hormone Secretion and Live Cell Imaging of Enteroendocrine Cells

Arianna Psichas*¹, Gwen Tolhurst*¹, Cheryl A. Brighton¹, Fiona M. Gribble¹, Frank Reimann¹

¹Metabolic Research Laboratories and MRC Metabolic Diseases Unit, Wellcome Trust-MRC Institute of Metabolic Science, University of Cambridge

High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast

Colby P. Fees¹, Cassi Estrem¹, Jeffrey K. Moore¹

¹Department of Cell and Developmental Biology, University of Colorado School of Medicine

Detection of Ligand-activated G Protein-coupled Receptor Internalization by Confocal Microscopy

Jingwen Yang¹, Yunjun Yan¹, Xiaowei Xiang¹, Yuchao Xu¹, Naiming Zhou², Tianming Wang¹

¹National Engineering Research Center of Marine Facilities Aquaculture, Marine Science College, Zhejiang Ocean University, ²Institute of Biochemistry, College of Life Sciences, Zhejiang University

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