JoVE Journal

Cancer Research

JoVE Cancer Research includes experimental approaches and methods to understand, detect, treat, and prevent cancer.

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PET and MRI Guided Irradiation of a Glioblastoma Rat Model Using a Micro-irradiator

Julie Bolcaen¹, Benedicte Descamps², Tom Boterberg³, Christian Vanhove², Ingeborg Goethals¹

¹Department of Nuclear Medicine, Ghent University Hospital, ²IBiTech-MEDISIP, Department of Electronics and Information Systems, Ghent University, ³Department of Radiation Oncology, Ghent University Hospital

Ex Vivo Imaging of Resident CD8 T Lymphocytes in Human Lung Tumor Slices Using Confocal Microscopy

Elisa Peranzoni*¹, Houcine Bougherara*¹, Sarah Barrin*¹, Audrey Mansuet-Lupo^2,3, Marco Alifano⁴, Diane Damotte^2,3, Emmanuel Donnadieu¹

¹Institut Cochin, Inserm U1016-CNRS UMR8104, Université Paris Descartes, ²Department of Pathology, Paris Centre University Hospitals, Université Paris Descartes, ³INSERM U1138, Cancer and Immune Escape, Cordeliers Research Center, University Pierre and Marie Curie, ⁴Department of Thoracic Surgery, Paris Centre University Hospitals, University Paris Descartes

Protocol for HER2 FISH Using a Non-cross-linking, Formalin-free Tissue Fixative to Combine Advantages of Cryo-preservation and Formalin Fixation

Martina Loibner^1,2, Lisa Oberauner-Wappis^1,2, Christian Viertler², Daniel Groelz³, Kurt Zatloukal^1,2

¹Christian Doppler Laboratory for Biospecimen Research and Biobanking Technologies, Institute of Pathology, Medical University Graz, ²Institute of Pathology, Medical University Graz, ³Research and Development, Qiagen GmbH

Characterization of Tumor Cells Using a Medical Wire for Capturing Circulating Tumor Cells: A 3D Approach Based on Immunofluorescence and DNA FISH

Giulia Gallerani¹, Claudia Cocchi², Martine Bocchini³, Filippo Piccinini¹, Francesco Fabbri¹

¹Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, ²Associazione Annastaccatolisa Onlus, ³Nuclear Medicine Unit, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS

Whole-body PET/MRI of Pediatric Patients: The Details That Matter

Anuj Pareek¹, Anne M. Muehe¹, Ashok J. Theruvath¹, Praveen K. Gulaka¹, Sheri L. Spunt², Heike E. Daldrup-Link¹

¹Department of Radiology, Pediatric Molecular Imaging Program at Stanford (PEDS-MIPS), Stanford University, ²Department of Pediatrics, Stanford University

Serum and Plasma Copy Number Detection Using Real-time PCR

Samanta Salvi¹, Vincenza Conteduca¹, Filippo Martignano¹, Giorgia Gurioli¹, Daniele Calistri¹, Valentina Casadio¹

¹Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS

Looking for Driver Pathways of Acquired Resistance to Targeted Therapy: Drug Resistant Subclone Generation and Sensitivity Restoring by Gene Knock-down

Chiara Arienti¹, Sara Pignatta¹, Michele Zanoni¹, Michela Cortesi¹, Alice Zamagni¹, Filippo Piccinini², Anna Tesei¹

¹Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, ²Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS

Live-imaging of Breast Epithelial Cell Migration After the Transient Depletion of TIP60

Yanzhou Zhang¹, Grace SuShin Chia¹, Cheng Yong Tham¹, Sudhakar Jha^1,2

¹Cancer Science Institute of Singapore, National University of Singapore, ²Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore

Using the Dot Assay to Analyze Migration of Cell Sheets

Christina H. Stuelten¹

¹Laboratory of Cellular and Molecular Biology, National Cancer Institute, National Institutes of Health

Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment

Marta H. Kubala^1,2, Yves A. DeClerck^1,2,3

¹Division of Hematology, Oncology and Blood and Marrow Transplantation, Department of Pediatrics, University of Southern California, ²The Saban Research Institute of Children's Hospital Los Angeles, ³Department of Biochemistry and Molecular Medicine, University of Southern California

Evaluation of the Cell Invasion and Migration Process: A Comparison of the Video Microscope-based Scratch Wound Assay and the Boyden Chamber Assay

Jean-Baptiste Guy*^1,2, Sophie Espenel*^1,2, Alexis Vallard^1,2, Priscillia Battiston-Montagne¹, Anne-Sophie Wozny^1,3, Dominique Ardail^1,3, Gersende Alphonse^1,3, Chloé Rancoule^1,2, Claire Rodriguez-Lafrasse^1,3, Nicolas Magne^1,2

¹UMR CNRS 5822 /IN2P3, IPNL, PRISME, Laboratoire de Radiobiologie Cellulaire et Moléculaire, Faculté de Médecine Lyon-Sud, Université Lyon 1, ²Département de Radiothérapie, Institut de Cancérologie de la Loire - Lucien Neuwirth, ³Hospices Civils de Lyon, Centre Hospitalier Lyon-Sud

Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation

Magdalena Delgado*¹, Anisha Kothari*¹, Walter N. Hittelman², Timothy C. Chambers¹

¹Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, ²Department of Experimental Therapeutics, University of Texas MD Anderson Cancer Center

Immunofluorescence Microscopy of γH2AX and 53BP1 for Analyzing the Formation and Repair of DNA Double-strand Breaks

Henning D. Popp¹, Susanne Brendel¹, Wolf-Karsten Hofmann¹, Alice Fabarius¹

¹Department of Hematology and Oncology, Medical Faculty Mannheim of the Heidelberg University

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier (MSC) for Lung Cancer Screening

Mavis Mensah*¹, Cristina Borzi*¹, Carla Verri¹, Paola Suatoni², Davide Conte¹, Ugo Pastorino², Fortunato Orazio¹, Gabriella Sozzi¹, Mattia Boeri¹

¹Department of Experimental Oncology and Molecular Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, ²Unit of Thoracic Surgery, Fondazione IRCCS Istituto Nazionale dei Tumori

One-step Protocol for Evaluation of the Mode of Radiation-induced Clonogenic Cell Death by Fluorescence Microscopy

Daijiro Kobayashi¹, Atsushi Shibata², Takahiro Oike¹, Takashi Nakano¹

¹Department of Radiation Oncology, Gunma University Graduate School of Medicine, ²Advanced Scientific Research Leaders Development Unit, Gunma University Graduate School of Medicine

Identification and Characterization of Metastatic Factors by Gene Transfer into the Novel RIP-Tag; RIP-tva Murine Model

George Zhang¹, Yudan Chi¹, Yi-Chieh Nancy Du¹

¹Department of Pathology and Laboratory Medicine, Weill Cornell Medicine

Evaluating In Vitro DNA Damage Using Comet Assay

Yanxin Lu^1,2, Yang Liu¹, Chunzhang Yang¹

¹Neuro-Oncology Branch, Center for Cancer Research, National Cancer Institute, ²Basic Medical Science Department, Zunyi Medical College-Zhuhai Campus

Sample Extraction and Simultaneous Chromatographic Quantitation of Doxorubicin and Mitomycin C Following Drug Combination Delivery in Nanoparticles to Tumor-bearing Mice

Rui Xue Zhang¹, Tian Zhang¹, King Chen¹, Ji Cheng¹, Paris Lai¹, Andrew M. Rauth², K. Sandy Pang¹, Xiao Yu Wu¹

¹Department of Pharmaceutical Sciences, University of Toronto, ²Departments of Medical Biophysics and Radiation Oncology, University of Toronto, Ontario Cancer Institute, University Health Network

A General Method for Detecting Nitrosamide Formation in the In Vitro Metabolism of Nitrosamines by Cytochrome P450s

Erik S. Carlson^1,2, Pramod Upadhyaya², Stephen S. Hecht²

¹Department of Pharmacology, University of Minnesota, ²Masonic Cancer Center, University of Minnesota

Development of a Human Preclinical Model of Osteoclastogenesis from Peripheral Blood Monocytes Co-cultured with Breast Cancer Cell Lines

Laura Mercatali¹, Chiara Spadazzi¹, Giacomo Miserocchi¹, Chiara Liverani¹, Alessandro De Vita¹, Alberto Bongiovanni¹, Federica Recine¹, Dino Amadori¹, Toni Ibrahim¹

¹Osteoncology and Rare Tumors Center, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST), IRCCS

Generation of Prostate Cancer Cell Models of Resistance to the Anti-mitotic Agent Docetaxel

Lisa Mohr¹, Marc Carceles-Cordon¹, Jungreem Woo¹, Carlos Cordon-Cardo¹, Josep Domingo-Domenech¹, Veronica Rodriguez-Bravo^1,2

¹Department of Pathology, Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, ²Department of Oncological Sciences, Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai

Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells

Nathaniel W Holton¹, Joel F Andrews¹, Natalie R Gassman¹

¹Department of Oncologic Sciences, University of South Alabama Mitchell Cancer Institute

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

Xiaoxing Lv¹, Meiru Zhao¹, Yuting Yi¹, Lucheng Zhang¹, Yanfang Guan¹, Tao Liu¹, Ling Yang¹, Rongrong Chen¹, Jianhui Ma¹, Xin Yi¹

¹Geneplus-Beijing Institute

Preparation and Metabolic Assay of 3-dimensional Spheroid Co-cultures of Pancreatic Cancer Cells and Fibroblasts

Pawan Noel¹, Ruben Muñoz¹, George W. Rogers², Andrew Neilson², Daniel D. Von Hoff¹, Haiyong Han¹

¹Molecular Medicine Division, Translational Genomics Research Institute, ²Agilent Technologies

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