JoVE Journal

Developmental Biology

JoVE Developmental Biology includes methods for the study of biological development at all levels, from cellular to whole organisms.

1 2 3 4 5 6 7 8 9…21 › 498 VIDEO ARTICLES

Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants

Megan Vaughan¹, Katja A. Lamia¹

¹Scripps Research, Department of Molecular Medicine

JoVE 60310

Generation of Multicellular Human Primary Endometrial Organoids

Alina R. Murphy*¹, Teerawat Wiwatpanit*², Zhenxiao Lu¹, Batzaya Davaadelger¹, J. Julie Kim¹

¹Department of Obstetrics and Gynecology, Northwestern University, ²National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA)

JoVE 60384

Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration

Sandra Guadalupe Gonzalez Malagon*^1,2, Lisa Dobson*^1,3, Anna M Lopez Muñoz¹, Marcus Dawson¹, William Barrell^1,3, Petros Marangos^2,4, Matthias Krause³, Karen J Liu¹

¹Centre for Craniofacial and Regenerative Biology, King's College London, ²Institute of Molecular Biology and Biotechnology, FORTH, Department of Biomedical Research, University of Ioannina, ³Randall Centre of Cell & Molecular Biophysics, King's College London, ⁴Department of Biological Applications and Technology, University of Ioannina

JoVE 60051

An Ectopic Chemokine Expression Model for Testing Macrophage Recruitment In Vivo

Yunyun Jiang¹, Jiahao Chen¹, Jin Xu²

¹Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, ²Division of Cell, Developmental and Integrative Biology, School of Medicine, South China University of Technology

JoVE 60161

Assay for Blood-brain Barrier Integrity in Drosophila melanogaster

Matthew J. Davis¹, Danielle Talbot¹, Jennifer Jemc¹

¹Department of Biology, Loyola University Chicago

JoVE 60233

Three-dimensional Angiogenesis Assay System using Co-culture Spheroids Formed by Endothelial Colony Forming Cells and Mesenchymal Stem Cells

Sajita Shah*¹, Hyunsook Lee*¹, Young Hwan Park¹, Eunkyeong Jeon¹, Hye Kyung Chung², Eun Sang Lee², Jae Hoon Shim², Kyu-Tae Kang¹

¹College of Pharmacy, Duksung Innovative Drug Center, Duksung Women's University, ²Korea Drug Development Platform using Radio-isotope, Korea Institute of Radiological & Medical Sciences

JoVE 60032

Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment

Matteo Da Ros*¹, Tiina Lehtiniemi*¹, Opeyemi Olotu¹, Oliver Meikar¹, Noora Kotaja¹

¹Institute of Biomedicine, University of Turku

JoVE 60271

Performing Human Skeletal Muscle Xenografts in Immunodeficient Mice

Kyla A. Britson^1,2, Aaron D. Black^2,3, Kathryn R. Wagner^1,2,3,4, Thomas E. Lloyd^1,2,4

¹Graduate Program in Cellular and Molecular Medicine, Johns Hopkins University School of Medicine, ²Department of Neurology, Johns Hopkins University School of Medicine, ³Kennedy Krieger Institute, ⁴Department of Neuroscience, Johns Hopkins University School of Medicine

JoVE 59966

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

Kinga Winczura¹, Pawel Grzechnik¹

¹School of Biosciences, University of Birmingham

JoVE 59700

CRISPR/Cas9 Technology in Restoring Dystrophin Expression in iPSC-Derived Muscle Progenitors

Yue Jin¹, Yan Shen¹, Xuan Su¹, Neal Weintraub¹, Yaoliang Tang¹

¹Medical College of Georgia, Augusta University

JoVE 59432

Electroporation Method for In Vivo Delivery of Plasmid DNA in the Adult Zebrafish Telencephalon

Tamara Durovic^1,2, Jovica Ninkovic^1,3,4

¹Institute of Stem Cell Research, Helmholtz Zentrum München, ²Graduate School of Systemic Neurosciences, Ludwig Maximilian University, ³Department of Cell Biology and Anatomy, Biomedical Center, Ludwig Maximilian University, ⁴Munich Cluster for Systems Neurology (SyNergy)

JoVE 60066

Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles

Sophia Dao¹, Kenton Jones¹, Tamira Elul¹

¹College of Osteopathic Medicine, Touro University California

JoVE 60123

Evaluation of Fertilization State by Tracing Sperm Nuclear Morphology in Arabidopsis Double Fertilization

Taro Takahashi¹, Tomoko Igawa¹

¹Graduate School of Horticulture, Chiba University

JoVE 59916

A Direct and Simple Method to Assess Drosophila melanogaster's Viability from Embryo to Adult

Antonio L. Rockwell¹, Isabel Beaver¹, Cintia F. Hongay¹

¹Department of Biology, Clarkson University

JoVE 59996

Isolation, Culture, Characterization, and Differentiation of Human Muscle Progenitor Cells from the Skeletal Muscle Biopsy Procedure

Brandon J. Gheller*¹, Jamie Blum*¹, Sharon Soueid-Baumgarten², Erica Bender¹, Benjamin D. Cosgrove², Anna Thalacker-Mercer¹

¹Division of Nutritional Sciences, Cornell University, ²Meinig School of Biomedical Engineering, Cornell University

JoVE 59580

Injecting Gryllus bimaculatus Eggs

Samantha K. Barry¹, Taro Nakamura², Yuji Matsuoka³, Christoph Straub⁴, Hadley W. Horch⁴, Cassandra G. Extavour⁵

¹Colby College, ²Division of Evolutionary Developmental Biology, National Institute for Basic Biology, ³Department of Biological Sciences, National University of Singapore, ⁴Department Biology and Department of Neuroscience, Bowdoin College, ⁵Department of Organismic and Evolutionary Biology and Department of Molecular and Cellular Biology, Harvard University

JoVE 59726

Human Egg Maturity Assessment and Its Clinical Application

Zuzana Holubcová^1,2, Drahomíra Kyjovská¹, Martina Martonová¹, Darja Páralová¹, Tereza Klenková¹, Soňa Kloudová¹

¹Reprofit International, Clinic of Reproductive Medicine, ²Department of Histology and Embryology, Faculty of Medicine, Masaryk University

JoVE 60058

Pan-myeloid Differentiation of Human Cord Blood Derived CD34⁺ Hematopoietic Stem and Progenitor Cells

Aditi Bapat¹, Nakia Keita¹, Shalini Sharma¹

¹Department of Basic Medical Sciences, College of Medicine-Phoenix, University of Arizona

JoVE 59836

A Multi-Omics Extraction Method for the In-Depth Analysis of Synchronized Cultures of the Green Alga Chlamydomonas reinhardtii

Umarah Mubeen¹, Lais Albuquerque Giraldi¹, Jessica Jüppner¹, Patrick Giavalisco^1,2

¹Max Planck Institute of Molecular Plant Physiology, ²Max Planck Institute of Biology of Ageing

JoVE 59547

Isolation of Specific Neuron Populations from Roundworm Caenorhabditis elegans

Edward M. Germany¹, Nataliya Zahayko¹, Oleh Khalimonchuk^1,2,3,4

¹Department of Biochemistry, University of Nebraska, ²Nebraska Redox Biology Center, University of Nebraska, ³Nebraska Center for Integrated Biomolecular Communication, University of Nebraska, ⁴Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center

JoVE 60145

Human Blastocyst Biopsy and Vitrification

Roberta Maggiulli¹, Adriano Giancani^1,2, Danilo Cimadomo¹, Filippo M. Ubaldi¹, Laura Rienzi¹

¹G.EN.E.R.A. Centers for Reproductive Medicine, ²DAHFMO, Unit of Histology and Medical Embryology, Sapienza University of Rome

JoVE 59625

Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells

Balta Lucendo-Villarin¹, Hassan Rashidi^1,2, Sharmin Alhaque^1,3, Lena Fischer^1,4, Jose Meseguer-Ripolles¹, Yu Wang¹, Cliona O'Farrelly⁴, Michael Themis³, David C. Hay¹

¹MRC Centre for Regenerative Medicine, University of Edinburgh, ²UCL Great Ormond Street Institute of Child Health, University College London, ³Division of Biosciences, Department of Life Sciences, College of Health and Life Sciences, Brunel University London, ⁴School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin

JoVE 59965

X-ray Diffraction of Intact Murine Skeletal Muscle as a Tool for Studying the Structural Basis of Muscle Disease

Weikang Ma¹, Thomas C. Irving¹

¹BioCAT, Dept. of Biological Sciences, Illinois Institute of Technology

JoVE 59559

Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation

Danielle Sambo¹, Jingling Li¹, Thomas Brickler¹, Sundari Chetty^1,2

¹Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, ²Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine

JoVE 59833

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Immunology and Infection

Biology

Genetics

Neuroscience

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