JoVE Journal

Developmental Biology

JoVE Developmental Biology includes methods for the study of biological development at all levels, from cellular to whole organisms.

1 2 3 4 5 6 7 8 9…18 › 420 VIDEO ARTICLES

Derivation and Differentiation of Canine Ovarian Mesenchymal Stem Cells

Amanda B.T. Hill^1,2, Jonathan E.B.T. Hill¹, Fabiana F. Bressan³, Maria Angélica Miglino⁴, Joaquim M. Garcia¹

¹The School of Agricultural and Veterinary Studies (FCAV), Jaboticabal, São Paulo State University (UNESP), ²CRRF, Université de Montréal, ³FZEA, University of São Paulo, ⁴FMVZ, University of São Paulo

JoVE 58163

Direct Lineage Reprogramming of Adult Mouse Fibroblast to Erythroid Progenitors

Melissa Ilsley¹, Sandra Capellera-Garcia¹, Kishori Dhulipala¹, Alban Johansson¹, Johan Flygare¹

¹Department of Molecular Medicine and Gene Therapy, Lund University

JoVE 58464

An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity

Mengrou Liu*¹, Chunsen Zhu*¹, Shun Bai*², Xin Li¹, Kaiqiang Fu¹, Lan Ye¹, Ke Zheng¹

¹State Key Laboratory of Reproductive Medicine, Nanjing Medical University, ²Center for Reproductive Medicine, Department of Obstetrics and Gynecology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China

JoVE 58512

Fluorimetric Techniques for the Assessment of Sperm Membranes

Alisa Komsky-Elbaz^1,2, Zvi Roth^1,2

¹Animal Sperm Research Center, Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, ²Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem

JoVE 58622

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

Patrick S. McGrath^1,2,3, Nicole Diette^1,2, Igor Kogut^1,2,3, Ganna Bilousova^1,2,3

¹Department of Dermatology, University of Colorado School of Medicine, Anschutz Medical Campus, ²Charles C. Gates Center for Regenerative Medicine, University of Colorado School of Medicine, Anschutz Medical Campus, ³Stem Cell Biobank and Disease Modeling Core, University of Colorado School of Medicine, Anschutz Medical Campus

JoVE 58687

Microfluidic Assay for the Assessment of Leukocyte Adhesion to Human Induced Pluripotent Stem Cell-derived Endothelial Cells (hiPSC-ECs)

Oleh V. Halaidych¹, Francijna van den Hil¹, Christine L. Mummery^1,2, Valeria V. Orlova¹

¹Department of Anatomy and Embryology, Leiden University Medical Center, ²Department of Applied Stem Cell Technologies, University of Twente

JoVE 58678

A Mouse Distraction Osteogenesis Model

Masahito Fujio¹, Yusuke Osawa², Masaki Matsushita², Kota Ogisu¹, Shuhei Tsuchiya¹, Hiroshi Kitoh², Hideharu Hibi¹

¹Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, ²Department of Orthopaedic Surgery, Nagoya University Graduate School of Medicine

JoVE 57925

High Frequency Ultrasound for the Analysis of Fetal and Placental Development In Vivo

Nicole Meyer¹, Thomas Schüler², Ana Claudia Zenclussen¹

¹Experimental Obstetrics and Gynecology, Medical Faculty, Otto-von-Guericke University, ²Institute of Molecular and Clinical Immunology, Medical Faculty, Otto-von-Guericke University

JoVE 58616

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence

Cally Xiao^1,2,3, Frank Nitsche⁴, Hisham Bazzi^2,3

¹Graduate Program in Pharmacology and Experimental Therapeutics, University of Cologne, ²Department of Dermatology and Venereology, University Hospital of Cologne, ³Cologne Cluster of Excellence in Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, ⁴Department of General Ecology, Institute for Zoology, Biocenter Cologne, University of Cologne

JoVE 58321

Probing Cell Mechanics with Bead-Free Optical Tweezers in the Drosophila Embryo

Claire Chardès*¹, Raphael Clement*¹, Olivier Blanc¹, Pierre-François Lenne¹

¹CNRS, Institut de Biologie du Développement de Marseille, Aix-Marseille Université

JoVE 57900

Visualize Drosophila Leg Motor Neuron Axons Through the Adult Cuticle

Wenyue Guan¹, Lalanti Venkatasubramanian², Myungin Baek³, Richard S. Mann², Jonathan Enriquez¹

¹Institut de Génomique Fonctionnelle de Lyon, ENS de Lyon, CNRS, ²Departments of Biochemistry and Molecular Biophysics, and Neuroscience, Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University, ³Neuroscience Program, NYU School of Medicine

JoVE 58365

Visualization of Tangential Cell Migration in the Developing Chick Optic Tectum

Yuji Watanabe¹, Chie Sakuma¹, Hiroyuki Yaginuma¹

¹Department of Neuroanatomy and Embryology, School of Medicine, Fukushima Medical University

JoVE 58506

Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells

Heidi Hongisto*¹, Meri Vattulainen*¹, Tanja Ilmarinen¹, Alexandra Mikhailova², Heli Skottman¹

¹BioMediTech Institute, Faculty of Medicine and Life Sciences, University of Tampere, ²Department of Ophthalmology, SILK, Faculty of Medicine and Life Sciences, University of Tampere

JoVE 58279

Cell Cycle Analysis in the C. elegans Germline with the Thymidine Analog EdU

Zuzana Kocsisova^1,2, Ariz Mohammad², Kerry Kornfeld¹, Tim Schedl²

¹Department of Developmental Biology, Washington University School of Medicine, St. Louis, Missouri, ²Department of Genetics, Washington University School of Medicine, St. Louis, Missouri

JoVE 58339

Micromanipulation of Chromosomes in Insect Spermatocytes

Nicolas K.H. Lin¹, Ryder Nance², Jane Szybist², Alan Cheville², Leocadia V. Paliulis³

¹Program in Cell Biology/Biochemistry, Bucknell University, ²Department of Electrical and Computer Engineering, Bucknell University, ³Biology Department, Bucknell University

JoVE 57359

Adult Zebrafish Injury Models to Study the Effects of Prednisolone in Regenerating Bone Tissue

Karina Geurtzen¹, Franziska Knopf^1,2

¹CRTD – Center for Regenerative Therapies Dresden, TU Dresden, ²Center for Healthy Aging, TU Dresden

JoVE 58429

Intravenous and Intra-amniotic In Utero Transplantation in the Murine Model

Nicholas J. Ahn¹, John D. Stratigis¹, Barbara E. Coons¹, Alan W. Flake¹, Hyun-Duck Nah-Cederquist², William H. Peranteau¹

¹Division of General, Thoracic, and Fetal Surgery, Center for Fetal Research, Children's Hospital of Philadelphia, ²Division of Plastic Reconstructive Surgery, Center for Fetal Research, Children's Hospital of Philadelphia

JoVE 58047

Use of Hematopoietic Stem Cell Transplantation to Assess the Origin of Myelodysplastic Syndrome

Yang Jo Chung¹, Ghanwa Khawaja¹, Karen M. Wolcott², Peter D. Aplan¹

¹Genetics Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, ²LGI Flow Cytometry Core Facility, Center for Cancer Research, National Cancer Institute, National Institutes of Health

JoVE 58140

A Hyperandrogenic Mouse Model to Study Polycystic Ovary Syndrome

Ping Xue¹, Zhiqiang Wang¹, Xiaomin Fu^1,2, Junjiang Wang^1,3, Gopika Punchhi¹, Andrew Wolfe^1,4, Sheng Wu^1,4,5

¹Department of Pediatrics, Johns Hopkins University School of Medicine, ²Department of Health, Beijing Military General Hospital, ³Southern Medical University, ⁴Department of Molecular and Cellular Physiology, Johns Hopkins University School of Medicine, ⁵Department of Gynecology and Obstetrics, Johns Hopkins University School of Medicine

JoVE 58379

Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells

Lin-Chen Li*^1,2, Xin-Xin Yu*^1,2, Yu-Wei Zhang*¹, Ye Feng*^1,3, Wei-Lin Qiu*^1,3, Cheng-Ran Xu¹

¹College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, ²Academy for Advanced Interdisciplinary Studies, Peking University, ³PKU-Tsinghua-NIBS Graduate Program, Peking University

JoVE 58000

An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing

Lijuan Du¹, Amy Zhou¹, Alex Sohr¹, Sougata Roy¹

¹Department of Cell Biology and Molecular Genetics, University of Maryland

JoVE 58268

Effectiveness of the Air Stripping in Two Salmonid Fish, Rainbow Trout (Oncorhynchus Mykiss) and Brown Trout (Salmo Trutta Morpha fario)

Radosław Kajetan Kowalski¹, Beata Sarosiek¹, Sylwia Judycka¹, Katarzyna Dryl¹, Joanna Grudniewska², Stefan Dobosz², Beata Irena Cejko¹

¹Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Science, ²Department of Salmonid Fish Research, The Stanisław Sakowicz Inland Fisheries Institute

JoVE 56894

A Familial Hypercholesterolemia Human Liver Chimeric Mouse Model Using Induced Pluripotent Stem Cell-derived Hepatocytes

Jiayin Yang*^1,2,6, Lai-Yung Wong*², Xiao-Yu Tian*³, Rui Wei^1,2, Wing-Hon Lai², Ka-Wing Au², Zhiwei Luo^4,5, Carl Ward^4,5, Wai-In Ho², David P. Ibañez^4,5, Hao Liu^4,5, Xichen Bao^4,5, Baoming Qin^4,5, Yu Huang³, Miguel A. Esteban^4,5,7, Hung-Fat Tse^1,2,6,7

¹Department of Medicine, University of Hong Kong-Shenzhen Hospital, ²The Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, University of Hong Kong, ³School of Biomedical Sciences, Institute of Vascular Medicine, Li Ka Shing Institute of Health Sciences, Chinese University of Hong Kong, ⁴Key Laboratory of Regenerative Biology of the Chinese Academy of Sciences, Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health and Guangzhou Medical University, ⁵Laboratory of RNA, Chromatin, and Human Disease, CAS Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, ⁶Research Centre of Heart, Brain, Hormone, and Healthy Ageing, Li Ka Shing Faculty of Medicine, University of Hong Kong, ⁷Hong Kong-Guangdong Stem Cell and Regenerative Medicine Research Centre, University of Hong Kong and Guangzhou Institutes of Biomedicine and Health

JoVE 57556

Engineering Transplantation-suitable Retinal Pigment Epithelium Tissue Derived from Human Embryonic Stem Cells

Karim Ben M'Barek^1,2,3, Walter Habeler^1,2,3, Alexandra Plancheron^1,2,3, Mohamed Jarraya⁴, Olivier Goureau⁵, Christelle Monville^1,2

¹U861, I-Stem, Association Française contre les Myopathies (AFM), Institut National de la Santé et de la Recherche Médicale (INSERM), ²U861, I-Stem, Association Française contre les Myopathies (AFM), Université Evry Val-d'Essonne (UEVE), ³I-Stem, Association Française contre les Myopathies (AFM), Centre pour L’Etude des Cellules Souches (CECS), ⁴Banque de tissus humain, Hôpital Saint Louis, Assistance Publique - Hôpitaux de Paris (AP-HP), ⁵Sorbonne Université, INSERM, CNRS, Institut de la Vision, F-75012

JoVE 58216

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