JoVE Journal

Developmental Biology

JoVE Developmental Biology includes methods for the study of biological development at all levels, from cellular to whole organisms.

1 2 3 4 5 6 7 8 9…17 › 400 VIDEO ARTICLES

An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing

Lijuan Du¹, Amy Zhou¹, Alex Sohr¹, Sougata Roy¹

¹Department of Cell Biology and Molecular Genetics, University of Maryland

JoVE 58268

Effectiveness of the Air Stripping in Two Salmonid Fish, Rainbow Trout (Oncorhynchus Mykiss) and Brown Trout (Salmo Trutta Morpha fario)

Radosław Kajetan Kowalski¹, Beata Sarosiek¹, Sylwia Judycka¹, Katarzyna Dryl¹, Joanna Grudniewska², Stefan Dobosz², Beata Irena Cejko¹

¹Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Science, ²Department of Salmonid Fish Research, The Stanisław Sakowicz Inland Fisheries Institute

JoVE 56894

A Familial Hypercholesterolemia Human Liver Chimeric Mouse Model Using Induced Pluripotent Stem Cell-derived Hepatocytes

Jiayin Yang*^1,2,6, Lai-Yung Wong*², Xiao-Yu Tian*³, Rui Wei^1,2, Wing-Hon Lai², Ka-Wing Au², Zhiwei Luo^4,5, Carl Ward^4,5, Wai-In Ho², David P. Ibañez^4,5, Hao Liu^4,5, Xichen Bao^4,5, Baoming Qin^4,5, Yu Huang³, Miguel A. Esteban^4,5,7, Hung-Fat Tse^1,2,6,7

¹Department of Medicine, University of Hong Kong-Shenzhen Hospital, ²The Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, University of Hong Kong, ³School of Biomedical Sciences, Institute of Vascular Medicine, Li Ka Shing Institute of Health Sciences, Chinese University of Hong Kong, ⁴Key Laboratory of Regenerative Biology of the Chinese Academy of Sciences, Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health and Guangzhou Medical University, ⁵Laboratory of RNA, Chromatin, and Human Disease, CAS Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, ⁶Research Centre of Heart, Brain, Hormone, and Healthy Ageing, Li Ka Shing Faculty of Medicine, University of Hong Kong, ⁷Hong Kong-Guangdong Stem Cell and Regenerative Medicine Research Centre, University of Hong Kong and Guangzhou Institutes of Biomedicine and Health

JoVE 57556

Engineering Transplantation-suitable Retinal Pigment Epithelium Tissue Derived from Human Embryonic Stem Cells

Karim Ben M'Barek^1,2,3, Walter Habeler^1,2,3, Alexandra Plancheron^1,2,3, Mohamed Jarraya⁴, Olivier Goureau⁵, Christelle Monville^1,2

¹U861, I-Stem, Association Française contre les Myopathies (AFM), Institut National de la Santé et de la Recherche Médicale (INSERM), ²U861, I-Stem, Association Française contre les Myopathies (AFM), Université Evry Val-d'Essonne (UEVE), ³I-Stem, Association Française contre les Myopathies (AFM), Centre pour L’Etude des Cellules Souches (CECS), ⁴Banque de tissus humain, Hôpital Saint Louis, Assistance Publique - Hôpitaux de Paris (AP-HP), ⁵Sorbonne Université, INSERM, CNRS, Institut de la Vision, F-75012

JoVE 58216

Defined Xeno-free and Feeder-free Culture Conditions for the Generation of Human iPSC-derived Retinal Cell Models

Amélie Slembrouck-Brec*¹, Céline Nanteau*¹, José-Alain Sahel¹, Olivier Goureau¹, Sacha Reichman¹

¹Institut de la Vision, Sorbonne Université, INSERM, CNRS, F-75012 Paris, France

JoVE 57795

Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization

Stephanie A. Michalski*¹, Sangappa B. Chadchan*¹, Emily S. Jungheim¹, Ramakrishna Kommagani¹

¹Department of Obstetrics and Gynecology, Center for Reproductive Health Sciences, Washington University School of Medicine

JoVE 57684

Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina

Ratnesh K. Singh¹, Pablo E. Diaz¹, François Binette¹, Igor O. Nasonkin¹

¹BioTime, Inc

JoVE 58274

Analysis of Epididymal Protein Synthesis and Secretion

Wei Zhou^1,2, Petra Sipilä³, Geoffry N. De Iuliis^1,2, Matthew D. Dun^2,4, Brett Nixon^1,2

¹Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, ²Hunter Medical Research Institute, ³Department of Physiology, Turku Center for Disease Modeling, Institute of Biomedicine, University of Turku, ⁴School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle

JoVE 58308

A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue

Zhenan Liu¹, Jie Wen^1,2, Xue Leng¹, Qian Zhou¹, Changkuo Zhou³, Huaqiang Zhao¹, Xunwei Wu^1,2

¹Shandong Provincial Key Laboratory of Oral Tissue Regeneration and Laboratory for Tissue Engineering and Regeneration, School of Stomatology, Shandong University, ²Suzhou Institute of Shandong University, ³Department of Urology, Qilu Hospital of Shandong University

JoVE 57784

Organotypic Culture Method to Study the Development Of Embryonic Chicken Tissues

Daniel D. T. Andrews¹, Tamara A. Franz-Odendaal¹

¹Department of Biology, Mount Saint Vincent University

JoVE 57619

A Cell-based Assay to Investigate Non-muscle Myosin II Contractility via the Folded-gastrulation Signaling Pathway in Drosophila S2R+ Cells

Kimberly A. Peters¹, Elizabeth Detmar¹, Liz Sepulveda², Corrina Del Valle², Ruth Valsquier², Anna Ritz², Stephen L. Rogers^1,3,4, Derek A. Applewhite²

¹Department of Biology, The University of North Carolina at Chapel Hill, ²Department of Biology, Reed College, ³Carolina Center for Genome Sciences, The University of North Carolina at Chapel Hill, ⁴Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill

JoVE 58325

Culture and Transfection of Zebrafish Primary Cells

Giulio Russo^1,2, Franziska Lehne¹, Sol M. Pose Méndez¹, Stefan Dübel², Reinhard W. Köster¹, Wiebke A. Sassen¹

¹Division of Cellular and Molecular Neurobiology, Zoological Institute, Braunschweig University of Technology, ²Department of Biotechnology, Institute of Biochemistry, Biotechnology and Bioinformatics, Braunschweig University of Technology

JoVE 57872

Synaptic Microcircuit Modeling with 3D Cocultures of Astrocytes and Neurons from Human Pluripotent Stem Cells

Caroline Cvetkovic¹, Nupur Basu¹, Robert Krencik¹

¹Center for Neuroregeneration, Department of Neurosurgery, Houston Methodist Research Institute

JoVE 58034

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence

Young Yeon Kim*^1,2, Jee-Hyun Um*^1,2, Jeanho Yun^1,2

¹Department of Biochemistry, College of Medicine, Dong-A University, ²Peripheral Neuropathy Research Center, College of Medicine, Dong-A University

JoVE 57890

Nanoparticle-mediated siRNA Gene-silencing in Adult Zebrafish Heart

Chenglu Xiao¹, Fang Wang², Junjie Hou¹, Xiaojun Zhu¹, Ying Luo², Jing-Wei Xiong¹

¹Beijing Key Laboratory of Cardiometabolic Molecular Medicine, State Key Laboratory of Natural and Biomimetic Drugs, Institute of Molecular Medicine, Peking University, ²Department of Biomedical Engineering, College of Engineering, Peking University

JoVE 58054

Semi-automated Production of Hepatocyte Like Cells from Pluripotent Stem Cells

Jose Meseguer-Ripolles¹, Baltasar Lucendo-Villarin¹, Yu Wang¹, David C. Hay¹

¹MRC Centre for Regenerative Medicine, University of Edinburgh

JoVE 57995

Caenorhabditis Sieve: A Low-tech Instrument and Methodology for Sorting Small Multicellular Organisms

Skyler Hunter*^1,2, Malabika Maulik*³, Courtney Scerbak⁴, Elena Vayndorf², Barbara E. Taylor⁵

¹Department of Biology and Wildlife, University of Alaska Fairbanks, ²Institute of Arctic Biology, University of Alaska Fairbanks, ³Department of Chemistry and Biochemistry, University of Alaska Fairbanks, ⁴Departments of Biology and Chemistry, Earlham College, ⁵Department of Biological Sciences, College of Natural Science and Mathematics, California State University Long Beach

JoVE 58014

Thin Film Composite Silicon Elastomers for Cell Culture and Skin Applications: Manufacturing and Characterization

Silviya Boyadzhieva*^1,2, Sarah C.L. Fischer*^1,2, Svenja Lösch^1,3, Angela Rutz¹, Eduard Arzt^1,2, Klaus Kruttwig¹

¹INM - Leibniz Institute for New Materials, ²Department of Materials Science and Engineering, Saarland University, ³University of Applied Sciences Kaiserslautern

JoVE 57573

Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos

María José Blanco*¹, Ana I.R. Learte*¹, Miguel A. Marchena¹, Emma Muñoz-Sáez¹, María Antonia Cid¹, Iván Rodríguez-Martín¹, Cristina Sánchez-Camacho^2,3

¹Faculty of Biomedical and Health Sciences, Universidad Europea de Madrid, ²Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), ³School of Doctoral Studies and Research, Universidad Europea de Madrid

JoVE 57785

Induction and Validation of Cellular Senescence in Primary Human Cells

Alejandra Hernandez-Segura¹, Simone Brandenburg¹, Marco Demaria¹

¹European Research Institute for the Biology of Aging, University of Groningen, University Medical Center Groningen

JoVE 57782

Ovarian Tissue Culture to Visualize Phenomena in Mouse Ovary

Kouji Komatsu¹, Akira Iwase^2,3, Tomohiko Murase², Satoru Masubuchi¹

¹Department of Physiology, Aichi Medical University, ²Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, ³Department of Maternal and Perinatal Medicine, Nagoya University Hospital

JoVE 57794

Generation of First Heart Field-like Cardiac Progenitors and Ventricular-like Cardiomyocytes from Human Pluripotent Stem Cells

Michael S. Yu*^1,2, Sean Spiering*¹, Alexandre R. Colas¹

¹Sanford Burnham Prebys Medical Discovery Institute, ²Department of Bioengineering, University of California at San Diego

JoVE 57688

Gait Analysis of Age-dependent Motor Impairments in Mice with Neurodegeneration

Christine M. Rostosky^1,2, Ira Milosevic^1,2

¹European Neuroscience Institute (ENI), ²University Medical Center Göttigen (UMG)

JoVE 57752

Two-step Approach to Explore Early- and Late-stages of Organ Formation in the Avian Model: The Thymus and Parathyroid Glands Organogenesis Paradigm

Marta Figueiredo¹, Hélia Neves¹

¹Instituto de Histologia e Biologia do Desenvolvimento, Faculdade de Medicina, Universidade de Lisboa

JoVE 57114

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