JoVE Journal

Developmental Biology

JoVE Developmental Biology includes methods for the study of biological development at all levels, from cellular to whole organisms.

1 2 3 4 5 6 7 8 9…19 › 433 VIDEO ARTICLES

Combining Histochemical Staining and Image Analysis to Quantify Starch in the Ovary Primordia of Sweet Cherry during Winter Dormancy

Erica Fadon¹, Javier Rodrigo¹

¹Instituto Agroalimentario de Aragón - IA2 (CITA-Universidad de Zaragoza), Centro de Investigación y Tecnología Agroalimentaria de Aragón

JoVE 58524

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells

Stefano Martellucci^1,2, Costantino Santacroce¹, Valeria Manganelli², Francesca Santilli^1,2, Luca Piccoli³, Michele Cassetta³, Roberta Misasi², Maurizio Sorice², Vincenzo Mattei^1,2

¹Laboratory of Experimental Medicine and Environmental Pathology, Sabina Universitas - Rieti University Hub, ²Department of Experimental Medicine, Sapienza University of Rome, ³Department of Science Dentistry and Maxillofacial, Sapienza University of Rome

JoVE 59282

Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation

Miles W. DeAngelis¹, Ruth I. Johnson¹

¹Department of Biology, Wesleyan University

JoVE 59299

In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines

Edwina P. Kisanga¹, Zhonghua Tang¹, Seth Guller¹, Shannon Whirledge¹

¹Department of Obstetrics, Gynecology and Reproductive Sciences, Yale School of Medicine

JoVE 58942

Wholemount In Situ Hybridization for Astyanax Embryos

Heidi Luc¹, Connor Sears¹, Andrew Raczka¹, Joshua B. Gross¹

¹Department of Biological Sciences, University of Cincinnati

JoVE 59114

Methods Collection

Assessment of Ultrastructural Neuroplasticity Parameters After In Utero Transduction of the Developing Mouse Brain and Spinal Cord

David Lutz*¹, Monika von Düring*¹, Franco Corvace¹, Luzie Augustinowski¹, Anne-Kathrin Trampe¹, Marzena Nowak², Eckart Förster¹

¹Department of Neuroanatomy and Molecular Brain Research, Ruhr University Bochum, ²Central Animal Facility of the Medical Faculty, Ruhr University Bochum

JoVE 59084

Microinjection-based System for In Vivo Implantation of Embryonic Cardiomyocytes in the Avian Embryo

Trevor Henley¹, Kandace Thomas¹, Michael Bressan¹

¹Department of Cell Biology and Physiology, McAllister Heart Institute, University of North Carolina at Chapel Hill

JoVE 59267

Isolation of Embryonic Tissues and Formation of Quail-Chicken Chimeric Organs Using The Thymus Example

Marta Figueiredo¹, Hélia Neves¹

¹Instituto de Histologia e Biologia do Desenvolvimento, Faculdade de Medicina, Universidade de Lisboa

JoVE 58965

Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants

Sota Iwatani¹, Makiko Yoshida², Keiji Yamana¹, Daisuke Kurokawa¹, Jumpei Kuroda¹, Khin Kyae Mon Thwin¹, Suguru Uemura¹, Satoru Takafuji¹, Nanako Nino¹, Tsubasa Koda, Masami Mizobuchi⁴, Masahiro Nishiyama¹, Kazumichi Fujioka¹, Hiroaki Nagase¹, Ichiro Morioka⁵, Kazumoto Iijima¹, Noriyuki Nishimura¹

¹Department of Pediatrics, Kobe University Graduate School of Medicine, ²Department of Pathology, Kobe Children's Hospital, ³Department of Pediatrics, Hyogo College of Medicine, ⁴Department of Developmental Pediatrics, Shizuoka Children's Hospital, ⁵Department of Pediatrics, Nihon University School of Medicine

JoVE 58806

Visualizing Cellular Gibberellin Levels Using the nlsGPS1 Förster Resonance Energy Transfer (FRET) Biosensor

Annalisa Rizza¹, Ankit Walia¹, Bijun Tang¹, Alexander M. Jones¹

¹Sainsbury Laboratory, University of Cambridge

JoVE 58739

Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells

Shino Mitsunaga¹, Keiko Shioda¹, Kurt J. Isselbacher¹, Jacob H. Hanna², Toshi Shioda¹

¹Center for Cancer Research, Massachusetts General Hospital, ²Department of Molecular Genetics, Weizmann Institute of Science

JoVE 58297

Induction of Endothelial Differentiation in Cardiac Progenitor Cells Under Low Serum Conditions

Michika Mochizuki*¹, Giacomo Della Verde*¹, Habiba Soliman¹, Otmar Pfister^1,2, Gabriela M. Kuster^1,2

¹Department of Biomedicine, University Hospital and University of Basel, ²Division of Cardiology, University Hospital Basel

JoVE 58370

An In Vitro Model of a Parallel-Plate Perfusion System to Study Bacterial Adherence to Graft Tissues

Bartosz Ditkowski¹, Tiago R Veloso¹, Martyna Bezulska-Ditkowska^1,2, Andreas Lubig³, Stefan Jockenhoevel³, Petra Mela³, Ramadan Jashari⁴, Marc Gewillig¹, Bart Meyns⁵, Marc F Hoylaerts², Ruth Heying¹

¹Cardiovascular Developmental Biology, Department of Cardiovascular Sciences, KU Leuven, ²Centre for Molecular and Vascular Biology, Department of Cardiovascular Sciences, KU Leuven, ³Department of Biohybrid & Medical Textiles, AME - Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, ⁴European Homograft Bank, Saint Jean Clinique, ⁵Division of Clinical Cardiac Surgery, Department of Cardiovascular Sciences, KU Leuven

JoVE 58476

Derivation and Differentiation of Canine Ovarian Mesenchymal Stem Cells

Amanda B.T. Hill^1,2, Jonathan E.B.T. Hill¹, Fabiana F. Bressan³, Maria Angélica Miglino⁴, Joaquim M. Garcia¹

¹The School of Agricultural and Veterinary Studies (FCAV), Jaboticabal, São Paulo State University (UNESP), ²CRRF, Université de Montréal, ³FZEA, University of São Paulo, ⁴FMVZ, University of São Paulo

JoVE 58163

Direct Lineage Reprogramming of Adult Mouse Fibroblast to Erythroid Progenitors

Melissa Ilsley¹, Sandra Capellera-Garcia¹, Kishori Dhulipala¹, Alban Johansson¹, Johan Flygare¹

¹Department of Molecular Medicine and Gene Therapy, Lund University

JoVE 58464

An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity

Mengrou Liu*¹, Chunsen Zhu*¹, Shun Bai*², Xin Li¹, Kaiqiang Fu¹, Lan Ye¹, Ke Zheng¹

¹State Key Laboratory of Reproductive Medicine, Nanjing Medical University, ²Center for Reproductive Medicine, Department of Obstetrics and Gynecology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China

JoVE 58512

Fluorimetric Techniques for the Assessment of Sperm Membranes

Alisa Komsky-Elbaz^1,2, Zvi Roth^1,2

¹Animal Sperm Research Center, Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, ²Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem

JoVE 58622

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

Patrick S. McGrath^1,2,3, Nicole Diette^1,2, Igor Kogut^1,2,3, Ganna Bilousova^1,2,3

¹Department of Dermatology, University of Colorado School of Medicine, Anschutz Medical Campus, ²Charles C. Gates Center for Regenerative Medicine, University of Colorado School of Medicine, Anschutz Medical Campus, ³Stem Cell Biobank and Disease Modeling Core, University of Colorado School of Medicine, Anschutz Medical Campus

JoVE 58687

Microfluidic Assay for the Assessment of Leukocyte Adhesion to Human Induced Pluripotent Stem Cell-derived Endothelial Cells (hiPSC-ECs)

Oleh V. Halaidych¹, Francijna van den Hil¹, Christine L. Mummery^1,2, Valeria V. Orlova¹

¹Department of Anatomy and Embryology, Leiden University Medical Center, ²Department of Applied Stem Cell Technologies, University of Twente

JoVE 58678

A Mouse Distraction Osteogenesis Model

Masahito Fujio¹, Yusuke Osawa², Masaki Matsushita², Kota Ogisu¹, Shuhei Tsuchiya¹, Hiroshi Kitoh², Hideharu Hibi¹

¹Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, ²Department of Orthopaedic Surgery, Nagoya University Graduate School of Medicine

JoVE 57925

High Frequency Ultrasound for the Analysis of Fetal and Placental Development In Vivo

Nicole Meyer¹, Thomas Schüler², Ana Claudia Zenclussen¹

¹Experimental Obstetrics and Gynecology, Medical Faculty, Otto-von-Guericke University, ²Institute of Molecular and Clinical Immunology, Medical Faculty, Otto-von-Guericke University

JoVE 58616

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence

Cally Xiao^1,2,3, Frank Nitsche⁴, Hisham Bazzi^2,3

¹Graduate Program in Pharmacology and Experimental Therapeutics, University of Cologne, ²Department of Dermatology and Venereology, University Hospital of Cologne, ³Cologne Cluster of Excellence in Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, ⁴Department of General Ecology, Institute for Zoology, Biocenter Cologne, University of Cologne

JoVE 58321

Probing Cell Mechanics with Bead-Free Optical Tweezers in the Drosophila Embryo

Claire Chardès*¹, Raphael Clement*¹, Olivier Blanc¹, Pierre-François Lenne¹

¹CNRS, Institut de Biologie du Développement de Marseille, Aix-Marseille Université

JoVE 57900

Visualize Drosophila Leg Motor Neuron Axons Through the Adult Cuticle

Wenyue Guan¹, Lalanti Venkatasubramanian², Myungin Baek³, Richard S. Mann², Jonathan Enriquez¹

¹Institut de Génomique Fonctionnelle de Lyon, ENS de Lyon, CNRS, ²Departments of Biochemistry and Molecular Biophysics, and Neuroscience, Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University, ³Neuroscience Program, NYU School of Medicine

JoVE 58365

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