JoVE Journal

Developmental Biology

JoVE Developmental Biology includes methods for the study of biological development at all levels, from cellular to whole organisms.

1 2 3 4 5 6 7 8 9…16 › 384 VIDEO ARTICLES

Caenorhabditis Sieve: A Low-tech Instrument and Methodology for Sorting Small Multicellular Organisms

Skyler Hunter*^1,2, Malabika Maulik*³, Courtney Scerbak⁴, Elena Vayndorf², Barbara E. Taylor⁵

¹Department of Biology and Wildlife, University of Alaska Fairbanks, ²Institute of Arctic Biology, University of Alaska Fairbanks, ³Department of Chemistry and Biochemistry, University of Alaska Fairbanks, ⁴Departments of Biology and Chemistry, Earlham College, ⁵Department of Biological Sciences, College of Natural Science and Mathematics, California State University Long Beach

JoVE 58014

Thin Film Composite Silicon Elastomers for Cell Culture and Skin Applications: Manufacturing and Characterization

Silviya Boyadzhieva*^1,2, Sarah C.L. Fischer*^1,2, Svenja Lösch^1,3, Angela Rutz¹, Eduard Arzt^1,2, Klaus Kruttwig¹

¹INM - Leibniz Institute for New Materials, ²Department of Materials Science and Engineering, Saarland University, ³University of Applied Sciences Kaiserslautern

JoVE 57573

Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos

María José Blanco*¹, Ana I.R. Learte*¹, Miguel A. Marchena¹, Emma Muñoz-Sáez¹, María Antonia Cid¹, Iván Rodríguez-Martín¹, Cristina Sánchez-Camacho^2,3

¹Faculty of Biomedical and Health Sciences, Universidad Europea de Madrid, ²Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), ³School of Doctoral Studies and Research, Universidad Europea de Madrid

JoVE 57785

Induction and Validation of Cellular Senescence in Primary Human Cells

Alejandra Hernandez-Segura¹, Simone Brandenburg¹, Marco Demaria¹

¹European Research Institute for the Biology of Aging, University of Groningen, University Medical Center Groningen

JoVE 57782

Ovarian Tissue Culture to Visualize Phenomena in Mouse Ovary

Kouji Komatsu¹, Akira Iwase^2,3, Tomohiko Murase², Satoru Masubuchi¹

¹Department of Physiology, Aichi Medical University, ²Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, ³Department of Maternal and Perinatal Medicine, Nagoya University Hospital

JoVE 57794

Generation of First Heart Field-like Cardiac Progenitors and Ventricular-like Cardiomyocytes from Human Pluripotent Stem Cells

Michael S. Yu*^1,2, Sean Spiering*¹, Alexandre R. Colas¹

¹Sanford Burnham Prebys Medical Discovery Institute, ²Department of Bioengineering, University of California at San Diego

JoVE 57688

Gait Analysis of Age-dependent Motor Impairments in Mice with Neurodegeneration

Christine M. Rostosky^1,2, Ira Milosevic^1,2

¹European Neuroscience Institute (ENI), ²University Medical Center Göttigen (UMG)

JoVE 57752

Two-step Approach to Explore Early- and Late-stages of Organ Formation in the Avian Model: The Thymus and Parathyroid Glands Organogenesis Paradigm

Marta Figueiredo¹, Hélia Neves¹

¹Instituto de Histologia e Biologia do Desenvolvimento, Faculdade de Medicina, Universidade de Lisboa

JoVE 57114

Methods for the Study of Regeneration in Stentor

Athena Lin¹, Tatyana Makushok¹, Ulises Diaz¹, Wallace F. Marshall¹

¹Department of Biochemistry and Biophysics, University of California San Francisco

JoVE 57759

Preparing Developing Peripheral Olfactory Tissue for Molecular and Immunohistochemical Analysis in Drosophila

Scott Barish¹, Pelin C. Volkan¹

¹Department of Biology, Duke University

JoVE 57716

Zygotic Fluorescence Recovery After Photo-bleaching Analysis for Chromatin Looseness That Allows Full-term Development

Masatoshi Ooga^1,3, Satoshi Funaya², Fugaku Aoki², Teruhiko Wakayama^1,3

¹Faculty of Life and Environmental Sciences, Department of Biotechnology, University of Yamanashi, ²Department of Integrated Bioscience, Graduate School of Frontier Sciences, University of Tokyo, ³Advanced Biotechnology Center, University of Yamanashi

JoVE 57068

Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency

Tea Soon Park*¹, Ludovic Zimmerlin*¹, Rebecca Evans-Moses¹, Elias T. Zambidis¹

¹Department of Oncology, Division of Pediatric Oncology and Institute for Cell Engineering, Johns Hopkins School of Medicine

JoVE 57921

CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer

Weijun Feng*¹, Lena Herbst*², Peter Lichter², Stefan M. Pfister^3,4,5, Hai-Kun Liu¹, Daisuke Kawauchi^3,4

¹Division of Molecular Neurogenetics, German Cancer Research Center (DKFZ), DKFZ-ZMBH Alliance, ²Division of Molecular Genetics, German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK), ³Hopp-Children's Cancer Center at the NCT Heidelberg (KiTZ), ⁴Division of Pediatric Neurooncology, German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK), ⁵Department of Pediatric Hematology and Oncology, Heidelberg University Hospital

JoVE 57311

Preparation of Drosophila Larval Samples for Gas Chromatography-Mass Spectrometry (GC-MS)-based Metabolomics

Hongde Li¹, Jason M. Tennessen¹

¹Department of Biology, Indiana University

JoVE 57847

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

Andrew S. Riching¹, Yuanbiao Zhao¹, Yingqiong Cao¹, Pilar Londono¹, Hongyan Xu², Kunhua Song¹

¹Division of Cardiology, Department of Medicine, University of Colorado Anschutz Medical Campus, ²Department of Population Health Sciences, Medical College of Georgia, Augusta University

JoVE 57687

Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro

Marissa A. Scavuzzo¹, Jessica Teaw^2,3,4, Diane Yang^2,3,4, Malgorzata Borowiak^1,2,3,4,5

¹Program in Developmental Biology, Baylor College of Medicine, ²Center for Cell and Gene Therapy, Texas Children's Hospital, and Houston Methodist Hospital, Baylor College of Medicine, ³Molecular and Cellular Biology Department, Baylor College of Medicine, ⁴Stem Cell and Regenerative Medicine Center, Baylor College of Medicine, ⁵McNair Medical Institute, Baylor College of Medicine

JoVE 57599

Three-dimensional Organotypic Cultures of Vestibular and Auditory Sensory Organs

Ksenia Gnedeva^1,2, A. J. Hudspeth³, Neil Segil^1,2

¹Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine of the University of Southern California, ²Caruso Department of Otolaryngology-Head and Neck Surgery, Keck School of Medicine of the University of Southern California, ³Howard Hughes Medical Institute and Laboratory of Sensory Neuroscience, The Rockefeller University

JoVE 57527

Partial Lobular Hepatectomy: A Surgical Model for Morphologic Liver Regeneration

Jonathan M. Tsai^1,2, Irving L Weissman^1,2, Yuval Rinkevich^3,4

¹Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, ²Department of Developmental Biology, Stanford University School of Medicine, ³Comprehensive Pneumology Center, Institute of Lung Biology and Disease, Helmholtz Zentrum München, ⁴German Center for Lung Research (DZL)

JoVE 57302

CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells

Benjamin L. Kidder^1,2

¹Department of Oncology, Wayne State University School of Medicine, ²Karmanos Cancer Institute, Wayne State University School of Medicine

JoVE 57481

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery

Jui-Tung Liu¹, Mary Paige Lamprecht¹, Stephen A. Duncan¹

¹Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina

JoVE 57194

Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture

Brandon K. Hadland^1,2, Barbara Varnum-Finney¹, Cynthia Nourigat-Mckay¹, David Flowers¹, Irwin D. Bernstein^1,2

¹Clinical Research Division, Fred Hutchinson Cancer Research Center, ²Department of Pediatrics, Division of Pediatric Hematology/Oncology, University of Washington School of Medicine

JoVE 56973

Fetal Mouse Cardiovascular Imaging Using a High-frequency Ultrasound (30/45MHZ) System

Marlin Touma^1,2

¹Neonatal/Congenital Heart Laboratory, Cardiovascular Research Laboratory, David Geffen School of Medicine, University of California, Los Angeles, ²Children's Discovery and Innovation Institute, Department of Pediatrics, David Geffen School of Medicine, University of California, Los Angeles

JoVE 57210

Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries

Vera D. Rinaldi¹, Jordana C. Bloom², John C. Schimenti^1,2

¹Department of Biomedical Sciences, Cornell University, ²Department of Molecular Biology and Genetics, Cornell University

JoVE 57593

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer

Rika Azuma¹, Kei Miyamoto², Mami Oikawa³, Masayasu Yamada⁴, Masayuki Anzai^1,5

¹Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kindai University, ²Faculty of Biology-Oriented Science and Technology, Kindai University, ³Wellcome Trust/Cancer Research UK Gurdon Institute and Department of Zoology, University of Cambridge, ⁴Laboratory of Reproductive Biology, Graduate School of Agriculture, Kyoto University, ⁵Institute of Advanced Technology, Kindai University

JoVE 57036

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Immunology and Infection

Biology

Genetics

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