JoVE Journal

Developmental Biology

JoVE Developmental Biology includes methods for the study of biological development at all levels, from cellular to whole organisms.

‹1…9 10 11 12 13 14…25 › 590 VIDEO ARTICLES

Zygotic Fluorescence Recovery After Photo-bleaching Analysis for Chromatin Looseness That Allows Full-term Development

Masatoshi Ooga^1,3, Satoshi Funaya², Fugaku Aoki², Teruhiko Wakayama^1,3

¹Faculty of Life and Environmental Sciences, Department of Biotechnology, University of Yamanashi, ²Department of Integrated Bioscience, Graduate School of Frontier Sciences, University of Tokyo, ³Advanced Biotechnology Center, University of Yamanashi

JoVE 57068

Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency

Tea Soon Park*¹, Ludovic Zimmerlin*¹, Rebecca Evans-Moses¹, Elias T. Zambidis¹

¹Department of Oncology, Division of Pediatric Oncology and Institute for Cell Engineering, Johns Hopkins School of Medicine

JoVE 57921

CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer

Weijun Feng*¹, Lena Herbst*², Peter Lichter², Stefan M. Pfister^3,4,5, Hai-Kun Liu¹, Daisuke Kawauchi^3,4

¹Division of Molecular Neurogenetics, German Cancer Research Center (DKFZ), DKFZ-ZMBH Alliance, ²Division of Molecular Genetics, German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK), ³Hopp-Children's Cancer Center at the NCT Heidelberg (KiTZ), ⁴Division of Pediatric Neurooncology, German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK), ⁵Department of Pediatric Hematology and Oncology, Heidelberg University Hospital

JoVE 57311

Preparation of Drosophila Larval Samples for Gas Chromatography-Mass Spectrometry (GC-MS)-based Metabolomics

Hongde Li¹, Jason M. Tennessen¹

¹Department of Biology, Indiana University

JoVE 57847

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

Andrew S. Riching¹, Yuanbiao Zhao¹, Yingqiong Cao¹, Pilar Londono¹, Hongyan Xu², Kunhua Song¹

¹Division of Cardiology, Department of Medicine, University of Colorado Anschutz Medical Campus, ²Department of Population Health Sciences, Medical College of Georgia, Augusta University

JoVE 57687

Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro

Marissa A. Scavuzzo¹, Jessica Teaw^2,3,4, Diane Yang^2,3,4, Malgorzata Borowiak^1,2,3,4,5

¹Program in Developmental Biology, Baylor College of Medicine, ²Center for Cell and Gene Therapy, Texas Children's Hospital, and Houston Methodist Hospital, Baylor College of Medicine, ³Molecular and Cellular Biology Department, Baylor College of Medicine, ⁴Stem Cell and Regenerative Medicine Center, Baylor College of Medicine, ⁵McNair Medical Institute, Baylor College of Medicine

JoVE 57599

Three-dimensional Organotypic Cultures of Vestibular and Auditory Sensory Organs

Ksenia Gnedeva^1,2, A. J. Hudspeth³, Neil Segil^1,2

¹Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine of the University of Southern California, ²Caruso Department of Otolaryngology-Head and Neck Surgery, Keck School of Medicine of the University of Southern California, ³Howard Hughes Medical Institute and Laboratory of Sensory Neuroscience, The Rockefeller University

JoVE 57527

Partial Lobular Hepatectomy: A Surgical Model for Morphologic Liver Regeneration

Jonathan M. Tsai^1,2, Irving L Weissman^1,2, Yuval Rinkevich^3,4

¹Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, ²Department of Developmental Biology, Stanford University School of Medicine, ³Comprehensive Pneumology Center, Institute of Lung Biology and Disease, Helmholtz Zentrum München, ⁴German Center for Lung Research (DZL)

JoVE 57302

CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells

Benjamin L. Kidder^1,2

¹Department of Oncology, Wayne State University School of Medicine, ²Karmanos Cancer Institute, Wayne State University School of Medicine

JoVE 57481

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery

Jui-Tung Liu¹, Mary Paige Lamprecht¹, Stephen A. Duncan¹

¹Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina

JoVE 57194

Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture

Brandon K. Hadland^1,2, Barbara Varnum-Finney¹, Cynthia Nourigat-Mckay¹, David Flowers¹, Irwin D. Bernstein^1,2

¹Clinical Research Division, Fred Hutchinson Cancer Research Center, ²Department of Pediatrics, Division of Pediatric Hematology/Oncology, University of Washington School of Medicine

JoVE 56973

Fetal Mouse Cardiovascular Imaging Using a High-frequency Ultrasound (30/45MHZ) System

Marlin Touma^1,2

¹Neonatal/Congenital Heart Laboratory, Cardiovascular Research Laboratory, David Geffen School of Medicine, University of California, Los Angeles, ²Children's Discovery and Innovation Institute, Department of Pediatrics, David Geffen School of Medicine, University of California, Los Angeles

JoVE 57210

Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries

Vera D. Rinaldi¹, Jordana C. Bloom², John C. Schimenti^1,2

¹Department of Biomedical Sciences, Cornell University, ²Department of Molecular Biology and Genetics, Cornell University

JoVE 57593

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer

Rika Azuma¹, Kei Miyamoto², Mami Oikawa³, Masayasu Yamada⁴, Masayuki Anzai^1,5

¹Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kindai University, ²Faculty of Biology-Oriented Science and Technology, Kindai University, ³Wellcome Trust/Cancer Research UK Gurdon Institute and Department of Zoology, University of Cambridge, ⁴Laboratory of Reproductive Biology, Graduate School of Agriculture, Kyoto University, ⁵Institute of Advanced Technology, Kindai University

JoVE 57036

Assessment of the Effects of Endocrine Disrupting Compounds on the Development of Vertebrate Neural Network Function Using Multi-electrode Arrays

Karla R Sanchez¹, Mahlet D Mersha¹, Harbinder S Dhillon¹, Murali K Temburni¹

¹Department of Biological Sciences, Delaware State University

JoVE 56300

Visualization of Cellular Electrical Activity in Zebrafish Early Embryos and Tumors

Martin R. Silic¹, GuangJun Zhang^1,2

¹Department of Comparative Pathobiology, Purdue University, ²Purdue University Center for Cancer Research, Purdue Institute for Inflammation, Immunology and Infectious Diseases (PI4D), Purdue Institute for Integrative Neuroscience (PIIN), Purdue University

JoVE 57330

The Isolation and Culture of Primary Epicardial Cells Derived from Human Adult and Fetal Heart Specimens

Esther Dronkers¹, Asja T. Moerkamp¹, Tessa van Herwaarden¹, Marie-José Goumans¹, Anke M. Smits¹

¹Department of Molecular Cell Biology, Leiden University Medical Center

JoVE 57370

Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies

Xuesong Feng*¹, Faiza Naz*², Aster H. Juan¹, Stefania Dell'Orso², Vittorio Sartorelli¹

¹Laboratory of Muscle Stem Cells and Gene Regulation, National Institutes of Health, ²Office of Science and Technology, National Institute of Arthritis, Musculoskeletal and Skin Diseases (NIAMS), National Institutes of Health

JoVE 57212

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton

Sandeep Gopal¹, Roger Pocock¹

¹Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Department of Anatomy and Developmental Biology, Monash University

JoVE 57702

Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans

Kunal Baxi¹, Carlos E. de Carvalho¹

¹Department of Biology, University of Saskatchewan

JoVE 57414

Culturing of Retinal Pigment Epithelial Cells on an Ex Vivo Model of Aged Human Bruch's Membrane

Hui Cai¹, Jie Gong¹, Lucian V. Del Priore¹, Tongalp H. Tezel², Mark A. Fields¹

¹Department of Ophthalmology and Visual Science, Yale School of Medicine, ²Edward S. Harkness Eye Institute, Columbia University School of Medicine

JoVE 57084

Whole-mount Clearing and Staining of Arabidopsis Flower Organs and Siliques

Afif Hedhly¹, Hannes Vogler¹, Christof Eichenberger¹, Ueli Grossniklaus¹

¹Department of Plant and Microbial Biology, Zurich-Basel Plant Science Center, University of Zurich

JoVE 56441

Three-dimensional Inflammatory Human Tissue Equivalents of Gingiva

Li Xiao¹, Hisashi Okamura², Yasuo Kumazawa²

¹Department of Pharmacology, The Nippon Dental University, ²Department of Oral and Maxillofacial Surgery, The Nippon Dental University Hospital

JoVE 57157

Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution

Tomoko Yamazaki^1,2, Wenling Li¹, Yoh-Suke Mukouyama¹

¹Laboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, ²Earle A. Chiles Research Institute, Robert W. Franz Cancer Center, Providence Portland Medical Center

JoVE 57406

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Immunology and Infection

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