JoVE Journal

Developmental Biology

JoVE Developmental Biology includes methods for the study of biological development at all levels, from cellular to whole organisms.

‹1…11 12 13 14 15 16…25 › 590 VIDEO ARTICLES

Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea

Rabab Sharif¹, Shrestha Priyadarsini², Tyler G. Rowsey², Jian-Xing Ma³, Dimitrios Karamichos^1,2

¹Department of Cell Biology, University of Oklahoma Health Sciences Center, ²Department of Ophthalmology/Dean McGee Eye Institute, University of Oklahoma Health Sciences Center, ³Department of Physiology, Harold Hamm Diabetes Center, University of Oklahoma Health Sciences Center

JoVE 56308

Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells

Olivia Krefft*¹, Ammar Jabali*¹, Vira Iefremova¹, Philipp Koch^1,2,3, Julia Ladewig¹

¹Institute of Reconstructive Neurobiology, University of Bonn, ²Central Institute of Mental Health, University of Heidelberg/Medical Faculty Mannheim, ³Hector Institute for Translational Brain Research (HITBR gGmbH)

JoVE 56768

Mass Isolation and In Vitro Cultivation of Intramolluscan Stages of the Human Blood Fluke Schistosoma Mansoni

Nathalie Dinguirard¹, Codie Heinemann¹, Timothy P. Yoshino¹

¹Department of Pathobiological Sciences, University of Wisconsin, School of Veterinary Medicine

JoVE 56345

Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice

David E. Maridas¹, Elizabeth Rendina-Ruedy¹, Phuong T. Le¹, Clifford J. Rosen¹

¹Maine Medical Center Research Institute

JoVE 56750

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

Ashutosh Pandey¹, Hamed Jafar-Nejad^1,2

¹Department of Molecular and Human Genetics, Baylor College of Medicine, ²Program in Developmental Biology, Baylor College of Medicine

JoVE 56919

Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans

Ena Kolundzic¹, Stefanie Seelk¹, Baris Tursun¹

¹Berlin Institute for Medical Systems Biology, Max Delbrück Center for Molecular Medicine in the Helmholtz Association

JoVE 56889

A Rapid and Efficient Method to Dissect Pupal Wings of Drosophila Suitable for Immunodetections or PCR Assays

Carmen Bolatto¹, Cristina Parada¹, Victoria Colmenares¹

¹Departamento de Histología y Embriología, Facultad de Medicina, Laboratorio de Biología del Desarrollo

JoVE 55854

Generation and Culturing of Primary Human Keratinocytes from Adult Skin

Claus Johansen¹

¹Department of Dermatology, Aarhus University Hospital

JoVE 56863

Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids

David R. Hill¹, Sha Huang¹, Yu-Hwai Tsai¹, Jason R. Spence^1,2, Vincent B. Young^1,3

¹Department of Internal Medicine, Division of Gastroenterology, University of Michigan, ²Department of Cell and Developmental Biology, University of Michigan, ³Department of Internal Medicine, Division of Infectious Disease, University of Michigan

JoVE 56960

In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity

Shen Zhang¹, Yonggen Wu¹, Yimin Weng², Zhihui Xu¹, Wenmin Chen³, Dahan Zheng⁴, Wei Lin⁵, Jun Liu⁶, Ying Zhou^1,7

¹Reproductive Medicine Center, The First Affiliated Hospital of Wenzhou Medical University, ²Department of Orthopaedics, The Second Affiliated Hospital of Wenzhou Medical University, ³Department of Obstetrics, The First Affiliated Hospital of Wenzhou Medical University, ⁴School of Laboratory Medicine and Life Science, Wenzhou Medical University, ⁵School of Pharmaceutical Science, Wenzhou Medical University, ⁶Stem Cells and Genetic Engineering Group, AgriBioscience Research Centre, Department of Economic Development, Jobs, Transport and Resources, ⁷Department of Histology and Embryology, Wenzhou Medical University

JoVE 55641

Immuno-fluorescent Labeling of Microtubules and Centrosomal Proteins in Ex Vivo Intestinal Tissue and 3D In Vitro Intestinal Organoids

Deborah A. Goldspink¹, Zoe J. Matthews², Elizabeth K. Lund¹, Tom Wileman², Mette M. Mogensen¹

¹School of Biological Sciences, University of East Anglia, ²Norwich Medical School, University of East Anglia

JoVE 56662

Streamlined 3D Cerebellar Differentiation Protocol with Optional 2D Modification

Dwayne B. Holmes¹, Vivi M. Heine^1,2

¹Department of Pediatrics/Child Neurology, Amsterdam Neuroscience, VU University Medical Center, ²Department of Complex Trait Genetics, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije Universiteit Amsterdam

JoVE 56888

Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae

Felix Ellett¹, Daniel Irimia¹

¹BioMEMS Resource Center, Department of Surgery, Massachusetts General Hospital–Harvard Medical School–Shriners Burns Hospital

JoVE 56498

Using Fluorescence In Situ Hybridization (FISH) to Monitor the State of Arm Cohesion in Prometaphase and Metaphase I Drosophila Oocytes

Adrienne T. Perkins¹, Sharon E. Bickel¹

¹Department of Biological Sciences, Dartmouth College

JoVE 56802

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications

Matthias Becker^1,2, Janita A. Maring^1,2, Barbara Oberwallner², Benjamin Kappler², Oliver Klein^1,2, Volkmar Falk^2,3,4,5, Christof Stamm^2,3,4

¹Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, ²Berlin-Brandenburg Center for Regenerative Therapies (BCRT), ³German Center for Cardiovascular Research (DZHK), ⁴Deutsches Herzzentrum Berlin (DHZB), ⁵Department of Cardiovascular Surgery, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health

JoVE 56419

Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells (HSPCs) in Developing Zebrafish Embryos

A.C. Berrun¹, D.L. Stachura¹

¹Department of Biological Sciences, California State University, Chico

JoVE 56836

AFM and Microrheology in the Zebrafish Embryo Yolk Cell

Maria Marsal¹, Ignasi Jorba², Elena Rebollo¹, Tomas Luque², Daniel Navajas², Enrique Martín-Blanco¹

¹Instituto de Biología Molecular de Barcelona, Consejo Superior de Investigaciones Científicas, ²Institute for Bioengineering of Catalonia, Universitat de Barcelona and CIBER Enfermedades Respiratorias

JoVE 56224

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Jaroslav Slamecka¹, Javier Laurini², Troy Shirley¹, Simon Philipp Hoerstrup³, Benedikt Weber^3,4,5, Laurie Owen¹, Steven McClellan¹

¹Mitchell Cancer Institute, University of South Alabama, ²College of Medicine, University of South Alabama, ³Institute for Regenerative Medicine, University of Zurich, ⁴Department of Dermatology, University Hospital Zurich, ⁵Center for Applied Biotechnology and Molecular Medicine (CABMM), University of Zurich - Irchel Campus

JoVE 56003

Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification

Cui Lin¹, Dongxing Zhu², Greg Markby¹, Brendan M. Corcoran³, Colin Farquharson¹, Vicky E. Macrae¹

¹Developmental Biology, The Roslin Institute and R(D)SVS, University of Edinburgh, ²Guangzhou Institute of Cardiovascular Disease, The Second Affiliated Hospital, School of Basic Medical Sciences, Guangzhou Medical University, ³Clinical Sciences and R(D)SVS, University of Edinburgh

JoVE 56126

Collection of Post-mating Semen from the Female Reproductive Tract and Measurement of Semen Liquefaction in Mice

Shuai Li¹, Wipawee Winuthayanon¹

¹School of Molecular Biosciences, Center for Reproductive Biology, College of Veterinary Medicine, Washington State University

JoVE 56670

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence

Amanda N. Marra¹, Marisa Ulrich¹, Audra White¹, Meghan Springer¹, Rebecca A. Wingert¹

¹Department of Biological Sciences, University of Notre Dame

JoVE 56261

Dissection and Coronal Slice Preparation of Developing Mouse Pituitary Gland

Dongmei Cao¹, Xianhua Ma¹, Weiping J. Zhang¹, Zhifang Xie^1,2

¹Department of Pathophysiology, Second Military Medical University, ²Department of Cell Biology, Second Military Medical University

JoVE 56356

Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina

José M. Mateos¹, Gery Barmettler¹, Jana Doehner¹, Irene Ojeda Naharros², Bruno Guhl¹, Stephan C.F. Neuhauss², Andres Kaech¹, Ruxandra Bachmann-Gagescu^2,3, Urs Ziegler¹

¹Center for Microscopy and Image Analysis, University of Zurich, ²Institute for Molecular Life Sciences, University of Zurich, ³Institute for Medical Genetics, University of Zurich

JoVE 56113

Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains

Seth M. Kelly^1,2, Alexandra Elchert³, Michael Kahl^2,3

¹Program in Neuroscience, The College of Wooster, ²Department of Biology, The College of Wooster, ³Program in Biochemistry, Cellular, and Molecular Biology, The College of Wooster

JoVE 56174

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Immunology and Infection

Biology

Genetics

Neuroscience

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