JoVE Journal

Developmental Biology

JoVE Developmental Biology includes methods for the study of biological development at all levels, from cellular to whole organisms.

‹1…12 13 14 15 16 17…26 › 601 VIDEO ARTICLES

Using Fluorescence In Situ Hybridization (FISH) to Monitor the State of Arm Cohesion in Prometaphase and Metaphase I Drosophila Oocytes

Adrienne T. Perkins¹, Sharon E. Bickel¹

¹Department of Biological Sciences, Dartmouth College

JoVE 56802

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications

Matthias Becker^1,2, Janita A. Maring^1,2, Barbara Oberwallner², Benjamin Kappler², Oliver Klein^1,2, Volkmar Falk^2,3,4,5, Christof Stamm^2,3,4

¹Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, ²Berlin-Brandenburg Center for Regenerative Therapies (BCRT), ³German Center for Cardiovascular Research (DZHK), ⁴Deutsches Herzzentrum Berlin (DHZB), ⁵Department of Cardiovascular Surgery, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health

JoVE 56419

Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells (HSPCs) in Developing Zebrafish Embryos

A.C. Berrun¹, D.L. Stachura¹

¹Department of Biological Sciences, California State University, Chico

JoVE 56836

AFM and Microrheology in the Zebrafish Embryo Yolk Cell

Maria Marsal¹, Ignasi Jorba², Elena Rebollo¹, Tomas Luque², Daniel Navajas², Enrique Martín-Blanco¹

¹Instituto de Biología Molecular de Barcelona, Consejo Superior de Investigaciones Científicas, ²Institute for Bioengineering of Catalonia, Universitat de Barcelona and CIBER Enfermedades Respiratorias

JoVE 56224

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Jaroslav Slamecka¹, Javier Laurini², Troy Shirley¹, Simon Philipp Hoerstrup³, Benedikt Weber^3,4,5, Laurie Owen¹, Steven McClellan¹

¹Mitchell Cancer Institute, University of South Alabama, ²College of Medicine, University of South Alabama, ³Institute for Regenerative Medicine, University of Zurich, ⁴Department of Dermatology, University Hospital Zurich, ⁵Center for Applied Biotechnology and Molecular Medicine (CABMM), University of Zurich - Irchel Campus

JoVE 56003

Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification

Cui Lin¹, Dongxing Zhu², Greg Markby¹, Brendan M. Corcoran³, Colin Farquharson¹, Vicky E. Macrae¹

¹Developmental Biology, The Roslin Institute and R(D)SVS, University of Edinburgh, ²Guangzhou Institute of Cardiovascular Disease, The Second Affiliated Hospital, School of Basic Medical Sciences, Guangzhou Medical University, ³Clinical Sciences and R(D)SVS, University of Edinburgh

JoVE 56126

Collection of Post-mating Semen from the Female Reproductive Tract and Measurement of Semen Liquefaction in Mice

Shuai Li¹, Wipawee Winuthayanon¹

¹School of Molecular Biosciences, Center for Reproductive Biology, College of Veterinary Medicine, Washington State University

JoVE 56670

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence

Amanda N. Marra¹, Marisa Ulrich¹, Audra White¹, Meghan Springer¹, Rebecca A. Wingert¹

¹Department of Biological Sciences, University of Notre Dame

JoVE 56261

Dissection and Coronal Slice Preparation of Developing Mouse Pituitary Gland

Dongmei Cao¹, Xianhua Ma¹, Weiping J. Zhang¹, Zhifang Xie^1,2

¹Department of Pathophysiology, Second Military Medical University, ²Department of Cell Biology, Second Military Medical University

JoVE 56356

Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina

José M. Mateos¹, Gery Barmettler¹, Jana Doehner¹, Irene Ojeda Naharros², Bruno Guhl¹, Stephan C.F. Neuhauss², Andres Kaech¹, Ruxandra Bachmann-Gagescu^2,3, Urs Ziegler¹

¹Center for Microscopy and Image Analysis, University of Zurich, ²Institute for Molecular Life Sciences, University of Zurich, ³Institute for Medical Genetics, University of Zurich

JoVE 56113

Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains

Seth M. Kelly^1,2, Alexandra Elchert³, Michael Kahl^2,3

¹Program in Neuroscience, The College of Wooster, ²Department of Biology, The College of Wooster, ³Program in Biochemistry, Cellular, and Molecular Biology, The College of Wooster

JoVE 56174

Application of Aorta-gonad-mesonephros Explant Culture System in Developmental Hematopoiesis

Junhua Lv^1,2, Feng Liu^1,2

¹State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, ²University of Chinese Academy of Sciences

JoVE 56557

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

Carissa Dege¹, Christopher M. Sturgeon¹

¹Department of Internal Medicine, Hematology Division, Washington University in St. Louis

JoVE 55196

Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells

Leah P. Foltz¹, Dennis O. Clegg¹

¹Center for Stem Cell Biology and Engineering, University of California, Santa Barbara

JoVE 56274

Evaluation of Injury-induced Senescence and In Vivo Reprogramming in the Skeletal Muscle

Coralie Cazin¹, Aurelie Chiche¹, Han Li¹

¹Cellular Plasticity & Disease Modelling, Department of Developmental & Stem Cell Biology, CNRS UMR 3738, Institut Pasteur

JoVE 56201

A Novel Use of Three-dimensional High-frequency Ultrasonography for Early Pregnancy Characterization in the Mouse

Mary C. Peavey^1,4, Corey L. Reynolds², Maria M. Szwarc², William E. Gibbons¹, Cecilia T. Valdes¹, Francesco J. DeMayo*³, John P. Lydon*⁴

¹Devision of Repreoductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Baylor College of Medicine, ²Mouse Phenotyping Core, Baylor College of Medicine, ³Reproductive and Developmental Biology Laboratory, National Institute of Environmental Health Sciences, ⁴Department of Molecular and Cellular Biology, Baylor College of Medicine

JoVE 56207

Immobilization of Caenorhabditis elegans to Analyze Intracellular Transport in Neurons

Shinsuke Niwa¹

¹Frontier Research Institute for Interdisciplinary Sciences and Graduate School of Life Sciences, Tohoku University

JoVE 56690

Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos

Evan Bardot^1,2,3, Nikos Tzavaras⁴, Deanna L. Benson^5,6, Nicole C. Dubois^1,2,3

¹Cell, Developmental, and Regenerative Biology Department, Icahn School of Medicine at Mount Sinai, ²Mindich Child Health and Development Institute, Icahn School of Medicine at Mount Sinai, ³Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, ⁴Microscopy Core, Icahn School of Medicine at Mount Sinai, ⁵Department of Neuroscience, Icahn School of Medicine at Mount Sinai, ⁶Friedman Brain Institute, Icahn School of Medicine at Mount Sinai

JoVE 56446

In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye

Meng Li*^1,2, Ming Hao*^1,2, Dong Jiang³, Yanxi Chen⁴, Wen Wang^1,2

¹Cellular Biomedicine Group, China, ²Cellular Biomedicine Group, California, ³Department of Orthopedics, Shanghai TCM-Integrated Hospital, ⁴Department of Orthopedics, Shanghai East Hospital, Tongji University

JoVE 56273

Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae

Pei-Wen Liu¹, Jia-Bao Xu¹, Yun-Qiao Dong², Xiao-Guang Chen¹, Jin-Bao Gu¹

¹Department of Pathogen Biology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, ²Reproductive Medical Centre of Guangdong Women and Children's Hospital

JoVE 56121

The C. elegans Excretory Canal as a Model for Intracellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis in a Single Cell: labeling by GFP-fusions, RNAi Interaction Screen and Imaging

Nan Zhang^1,2, Edward Membreno¹, Susan Raj¹, Hongjie Zhang^1,3, Liakot A Khan¹, Verena Gobel¹

¹Mucosal Immunology and Biology Research Center, Developmental Biology and Genetics Core, Massachusetts General Hospital for Children, Harvard Medical School, ²College of Life Sciences, Jilin University, ³Faculty of Health Sciences, University of Macau

JoVE 56101

The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging

Nan Zhang^1,2, Liakot A Khan¹, Edward Membreno¹, Gholamali Jafari¹, Siyang Yan¹, Hongjie Zhang^1,3, Verena Gobel¹

¹Mucosal Immunology and Biology Research Center, Developmental Biology and Genetics Core, Massachusetts General Hospital, Harvard Medical School, ²College of Life Sciences, Jilin University, ³Faculty of Health Sciences, University of Macau

JoVE 56100

Establishment of Larval Zebrafish as an Animal Model to Investigate Trypanosoma cruzi Motility In Vivo

Veronica Akle¹, Nathalie Agudelo-Dueñas*^1,2, Maria A. Molina-Rodriguez*¹, Laurel Brianne Kartchner^1,3,4,6, Annette Marie Ruth^1,3,5,6, John M. González³, Manu Forero-Shelton²

¹Laboratory of Neurosciences and Circadian Rhythms, School of Medicine, Universidad de los Andes, ²Biophysics Group, Department of Physics, Universidad de los Andes, ³Laboratory of Basic Medical Sciences, School of Medicine, Universidad de los Andes, ⁴Department of Microbiology and Immunology, University of North Carolina, ⁵Notre Dame Initiative for Global Development, University of Notre Dame, ⁶USAID Research and Innovation Fellowship program

JoVE 56238

Methods for Imaging Intracellular pH of the Follicle Stem Cell Lineage in Live Drosophila Ovarian Tissue

Sumitra Tatapudy¹, Marimar Benitez¹, Todd Nystul¹

¹Departments of Anatomy and OB/GYN-RS, University of California, San Francisco

JoVE 56316

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Immunology and Infection

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