JoVE Journal

Developmental Biology

JoVE Developmental Biology covers research studies of biological development at all levels, ranging from cellular to whole organisms.

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Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells

Laura Gouder^1,2,3, Jean-Yves Tinevez⁴, Hany Goubran-Botros^1,2,3, Alexandra Benchoua⁵, Thomas Bourgeron^1,2,3, Isabelle Cloëz-Tayarani^1,2,3

¹Human Genetics and Cognitive Functions, Institut Pasteur, ²CNRS URA 2182 'Genes, synapses and cognition', Institut Pasteur, ³Human Genetics and Cognitive Functions, Université Paris Diderot, Sorbonne Paris Cité, ⁴Plateforme d' Imagerie Dynamique, Imagopole, CiTech, Institut Pasteur, ⁵Neuroplasticity and Therapeutics, CECS, I-STEM, AFM, Evry

JoVE 53197

Whole Retinal Explants from Chicken Embryos for Electroporation and Chemical Reagent Treatments

Shahrzad Shirazi Fard¹, Maria Blixt¹, Finn Hallböök¹

¹Department of Neuroscience, Biomedical Center, Uppsala University

JoVE 53202

Preparation of Mitochondrial Enriched Fractions for Metabolic Analysis in Drosophila

Eugenia Villa-Cuesta¹, David M. Rand²

¹Biology Department, Adelphi University, ²Department of Ecology and Evolutionary Biology, Brown University

JoVE 53149

Quantification of Colonic Stem Cell Mutations

Ryan D. Whetstone¹, Barry Gold¹

¹Department of Pharmaceutical Sciences, University of Pittsburgh

JoVE 53240

Method of Studying Palatal Fusion using Static Organ Culture

Isra Ibrahim¹, Maria J. Serrano¹, Kathy K.H. Svoboda¹

¹Department of Biomedical Sciences and Center for Craniofacial Research and Diagnosis, Texas A&M University Baylor College of Dentistry

JoVE 53063

Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes

Sandy S.C. Hung¹, Alice Pébay¹, Raymond C.B. Wong¹

¹Centre for Eye Research Australia & Department of Ophthalmology, University of Melbourne

JoVE 53174

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media

Ji-Hye Jung¹, Byung Soo Kim¹

¹Department of Biomedical Science, Graduate School of Medicine, Korea University

JoVE 53204

Kidney Regeneration in Adult Zebrafish by Gentamicin Induced Injury

Caramai N. Kamei¹, Yan Liu^1,2, Iain A. Drummond^1,3

¹Nephrology Division, Department of Medicine, Massachusetts General Hospital, ²Basic Sciences Division, Fred Hutchinson Cancer Research Center, ³Department of Genetics, Harvard Medical School

JoVE 51912

Microbead Implantation in the Zebrafish Embryo

Gary F. Gerlach¹, Elvin E. Morales¹, Rebecca A. Wingert¹

¹Department of Biological Sciences, University of Notre Dame

JoVE 52943

3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression

Sheena Pinto*¹, Hans-Jürgen Stark*², Iris Martin², Petra Boukamp², Bruno Kyewski¹

¹Division of Developmental Immunology, German Cancer Research Center (DKFZ), ²Genetics of Skin Carcinogenesis, German Cancer Research Center (DKFZ)

JoVE 52614

Ex Vivo Culture of Pharyngeal Arches to Study Heart and Muscle Progenitors and Their Niche

Peter Andersen¹, Chulan Kwon¹

¹Division of Cardiology, Institute for Cell Engineering, Johns Hopkins University School of Medicine

JoVE 52876

Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles

Paola L. Carvajal Monroy¹, Zipora Yablonka-Reuveni², Sander Grefte³, Anne Marie Kuijpers-Jagtman¹, Frank A.D.T.G. Wagener¹, Johannes W. Von den Hoff¹

¹Department of Orthodontics and Craniofacial Biology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, ²Department of Biological Structure, University of Washington School of Medicine, ³Department of Biochemistry, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center

JoVE 52802

Assessment of Endothelial Cell Migration After Exposure to Toxic Chemicals

Dirk Steinritz^1,2, Annette Schmidt^1,3, Frank Balszuweit¹, Horst Thiermann¹, Marwa Ibrahim³, Birgit Bölck³, Wilhelm Bloch³

¹Bundeswehr Institute of Pharmacology and Toxicology, ²Walther Straub Institute of Pharmacology and Toxicology, Ludwig-Maximilians-Universität München, ³Department of Molecular and Cellular Sports Medicine, German Sports University Cologne

JoVE 52768

Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle

Ikki Horiguchi¹, Yasuyuki Sakai¹

¹Center for International Research on Integrative Biomedical System, Institute of Industrial Science, The University of Tokyo

JoVE 52835

Use of the TetON System to Study Molecular Mechanisms of Zebrafish Regeneration

Daniel Wehner*¹, Christopher Jahn*¹, Gilbert Weidinger¹

¹Institute for Biochemistry and Molecular Biology, Ulm University

JoVE 52756

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation

Vaibhav Shinde*¹, Stefanie Klima*², Perumal Srinivasan Sureshkumar¹, Kesavan Meganathan¹, Smita Jagtap¹, Eugen Rempel³, Jörg Rahnenführer³, Jan Georg Hengstler⁴, Tanja Waldmann², Jürgen Hescheler¹, Marcel Leist*², Agapios Sachinidis*¹

¹Center of Physiology and Pathophysiology, Institute of Neurophysiology, University of Cologne, ²Department of Biology, University of Konstanz, ³Department of Statistics, Technical University of Dortmund, ⁴Leibniz Research Centre for Working Environment and Human Factors, Technical University of Dortmund

JoVE 52333

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays

Cyrille Ramond^1,3, Antonio Bandeira², Claire Berthault³, Pablo Pereira³, Ana Cumano³, Odile Burlen-Defranoux³

¹Research Center Growth and Signaling, INSERM U845, Institut Cochin, ²Unit for Biology of Lymphocyte Populations, Immunology Department, Institut Pasteur and CIMI, Unity of Treg Biology and Therapy, University of Pierre & Marie Curie, ³Unit for Lymphopoiesis, Immunology Department, INSERM U668, University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Institut Pasteur

JoVE 52795

Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula

Sergey Kurdyukov¹, Youhong Song¹, Terence W-Y Tiew¹, Xin-Ding Wang¹, Kim E. Nolan¹, Ray J. Rose¹

¹School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales, Australia

JoVE 52635

Establishment of a Clinically Relevant Ex Vivo Mock Cataract Surgery Model for Investigating Epithelial Wound Repair in a Native Microenvironment

Janice L. Walker¹, Brigid M. Bleaken¹, Iris M. Wolff¹, A. Sue Menko¹

¹Pathology, Anatomy and Cell Biology, Thomas Jefferson University

JoVE 52886

Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids

Viviana Meraviglia*¹, Alessandra Zanon*¹, Alexandros A. Lavdas¹, Christine Schwienbacher¹, Rosamaria Silipigni², Marina Di Segni², Huei-Sheng Vincent Chen³, Peter P. Pramstaller¹, Andrew A. Hicks¹, Alessandra Rossini¹

¹Center for Biomedicine, European Academy Bozen/Bolzano (EURAC), ²Laboratory of Medical Genetics, Fondazione IRCCS Ca´ Granda, Ospedale Maggiore Policlinico, ³Del E. Webb Center for Neuroscience, Aging & Stem Cell Research, Sanford-Burnham Medical Research Institute

JoVE 52885

Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System

Shenglan Li*^1,2, Haipeng Xue*^1,2, Bo Long^1,2,5, Li Sun^1,2,6, Tai Truong^1,2,4,7, Ying Liu^1,2,3

¹Department of Neurosurgery, The University of Texas Health Science Center at Houston, ²Center for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine, The University of Texas Health Science Center at Houston, ³The Senator Lloyd & B. A. Bentsen Center for Stroke Research, Brown Foundation Institute of Molecular Medicine, The University of Texas Health Science Center at Houston, ⁴Summer Research Program, Office of Educational Programs, The University of Texas Health Science Center at Houston, ⁵Department of Anesthesiology, Shengjing Hospital, China Medical University, ⁶Department of Oncology, Renji Hospital, Shanghai Jiaotong University School of Medicine, ⁷Biology Department, University of West Georgia

JoVE 52539

Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using Chick^GFPNeural Tube Grafting and Carbocyanine Dye DiI Injection

Jean-Marie Delalande^1,2, Nikhil Thapar¹, Alan J. Burns^1,3

¹Birth Defects Research Centre, UCL Institute of Child Health, ²Blizard Institute, Centre for Digestive Diseases, Queen Mary University of London, Barts and The London School of Medicine and Dentistry, ³Department of Clinical Genetics, Erasmus University Medical Center, Rotterdam