JoVE Journal

Developmental Biology

JoVE Developmental Biology includes methods for the study of biological development at all levels, from cellular to whole organisms.

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Isolation and Characterization of Mouse Antral Oocytes Based on Nucleolar Chromatin Organization

Manuela Monti¹, Carlo Alberto Redi^1,2

¹Research Center for Regenerative Medicine, Fondazione IRCCS Policlinico San Matteo, ²Department of Biology and Biotechnology "L. Spallanzani", University of Pavia

JoVE 53616

Murine Dermal Fibroblast Isolation by FACS

Graham G. Walmsley*^1,2, Zeshaan N. Maan*¹, Michael S. Hu*^1,2,3, David A. Atashroo¹, Alexander J. Whittam¹, Dominik Duscher¹, Ruth Tevlin¹, Owen Marecic¹, H. Peter Lorenz¹, Geoffrey C. Gurtner¹, Michael T. Longaker^1,2

¹Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Division of Plastic and Reconstructive Surgery, Stanford University School of Medicine, ²Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, ³Department of Surgery, John A. Burns School of Medicine, University of Hawai'i

JoVE 53430

Initiating Differentiation in Immortalized Multipotent Otic Progenitor Cells

Jadali Azadeh¹, Zhichao Song*¹, Alejandra S. Laureano*¹, Alana Toro-Ramos¹, Kelvin Kwan¹

¹Cell Biology & Neuroscience, Rutgers University

JoVE 53692

Step-specific Sorting of Mouse Spermatids by Flow Cytometry

Olivier Simard*¹, Frédéric Leduc*¹, Geneviève Acteau¹, Mélina Arguin¹, Marie-Chantal Grégoire¹, Marc-André Brazeau¹, Isabelle Marois², Martin V. Richter², Guylain Boissonneault¹

¹Department of Biochemistry, Université de Sherbrooke, ²Department of Medicine, Université de Sherbrooke

JoVE 53379

Methods for Precisely Localized Transfer of Cells or DNA into Early Postimplantation Mouse Embryos

Yali Huang¹, Ron Wilkie¹, Valerie Wilson¹

¹MRC Centre for Regenerative Medicine, School of Biological Sciences, University of Edinburgh

JoVE 53295

A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice

Aya Amitai-Lange*¹, Eran Berkowitz*², Anna Altshuler¹, Noora Dbayat², Waseem Nasser¹, Edith Suss-Toby³, Beatrice Tiosano², Ruby Shalom-Feuerstein¹

¹Department of Genetics and Developmental Biology, The Ruth and Bruce Rappaport Faculty of Medicine, Technion, Israel Institute of Technology, ²Department of Ophthalmology, Hillel Yaffe Medical Center, ³Bioimging Center, Biomedical Core Facility, The Ruth and Bruce Rappaport Faculty of Medicine, Technion, Israel Institute of Technology

JoVE 53370

Ex Vivo Culture of Chick Cerebellar Slices and Spatially Targeted Electroporation of Granule Cell Precursors

Michalina Hanzel¹, Richard J.T. Wingate¹, Thomas Butts²

¹MRC Centre of Developmental Neurobiology, King's College London, ²School of Biological and Chemical Sciences, Queen Mary, University of London

JoVE 53421

Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis

Jingting Li^1,2, George L. Sen^1,2

¹Department of Dermatology, UCSD Stem Cell Program, University of California, San Diego, ²Department of Cellular and Molecular Medicine, UCSD Stem Cell Program, University of California, San Diego

JoVE 53280

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients

Simon W. Fellgett¹, Simon A. Ramsbottom², Richard J. Maguire³, Stephen Cross⁴, Peter O'Toole⁵, Mary E. Pownall⁵

¹Department of Biomedical Science, The Bateson Centre, University of Sheffield, ²Institute of Genetic Medicine, Newcastle University, ³Department of Cardiovascular Science, The Bateson Centre, University of Sheffield, ⁴School of Biochemistry, University of Bristol, ⁵Biology Department, University of York

JoVE 53162

Assessment of Maternal Vascular Remodeling During Pregnancy in the Mouse Uterus

Jens Kieckbusch^1,2, Louise M. Gaynor^1,2, Francesco Colucci^1,2

¹Department of Obstetrics and Gynaecology, School of Clinical Medicine, University of Cambridge, ²Centre for Trophoblast Research, University of Cambridge

JoVE 53534

Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells

Irina Elcheva¹, Vera Brok-Volchanskaya¹, Igor Slukvin²

¹Primate Research Center, University of Wisconsin-Madison, ²Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health

JoVE 52910

Optimization of the Wound Scratch Assay to Detect Changes in Murine Mesenchymal Stromal Cell Migration After Damage by Soluble Cigarette Smoke Extract

Nicholas Cormier*¹, Alexander Yeo*¹, Elizabeth Fiorentino¹, Julia Paxson¹

¹Department of Biology, College of the Holy Cross

JoVE 53414

Geomagnetic Field (Gmf) and Plant Evolution: Investigating the Effects of Gmf Reversal on Arabidopsis thaliana Development and Gene Expression

Cinzia M. Bertea¹, Ravishankar Narayana¹, Chiara Agliassa¹, Christopher T. Rodgers², Massimo E. Maffei¹

¹Department of Life Sciences and Systems Biology, University of Turin, ²Radcliffe Department of Medicine, University of Oxford, John Radcliffe Hospital

JoVE 53286

Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye

Sarah J. Smith*^1,2, Eric J. Horstick*^3,4, Ann E. Davidson^1,2, James Dowling^1,2,4

¹Program in Genetics & Genome Biology, The Hospital for Sick Children, ²Department of Molecular Genetics, The University of Toronto, ³Program in Genomics of Differentiation, Eunice Kennedy Shriver National Institute of Child Health and Human Development, ⁴Departments of Pediatrics and Neurology, University of Michigan

JoVE 53183

Generation of Aggregates of Mouse Embryonic Stem Cells that Show Symmetry Breaking, Polarization and Emergent Collective Behaviour In Vitro

Peter Baillie-Johnson¹, Susanne Carina van den Brink^1,2, Tina Balayo¹, David Andrew Turner¹, Alfonso Martinez Arias¹

¹Department of Genetics, University of Cambridge, ²Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences

JoVE 53252

Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models

Lee Parry¹, Madeleine Young¹, Fatima El Marjou², Alan Richard Clarke¹

¹European Cancer Stem Cell Research Institute, Cardiff University, ²Institut Curie

JoVE 53429

Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5

Li Wang¹, Ziqing Liu¹, Chaoying Yin¹, Yang Zhou¹, Jiandong Liu¹, Li Qian¹

¹Department of Pathology and Laboratory Medicine, McAllister Heart Institute, University of North Carolina, Chapel Hill

JoVE 53426

Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions

Yoshikazu Kishino¹, Tomohisa Seki², Shinsuke Yuasa¹, Jun Fujita¹, Keiichi Fukuda¹

¹Department of Cardiology, Keio University School of Medicine, ²Department of Emergency and Critical Care Medicine, Keio University School of Medicine

JoVE 53225

Forward Genetic Approach to Uncover Stress Resistance Genes in Mice — A High-throughput Screen in ES Cells

Michael Ludwig¹, David Kitzenberg¹, Wallace S. Chick¹

¹Department of Cell and Developmental Biology, University of Colorado Denver

JoVE 53062

Assaying Blood Cell Populations of the Drosophila melanogaster Larva

Sophia Petraki¹, Brandy Alexander¹, Katja Brückner^1,2,3

¹Department of Cell and Tissue Biology, University of California San Francisco, ²Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, ³Cardiovascular Research Institute, University of California San Francisco

JoVE 52733

Delivery of In Vivo Acute Intermittent Hypoxia in Neonatal Rodents to Prime Subventricular Zone-derived Neural Progenitor Cell Cultures

Heather H. Ross¹, Milap S. Sandhu¹, Sharareh Sharififar¹, David D. Fuller¹

¹Department of Physical Therapy, University of Florida

JoVE 52527

Efficient Gene Transfer in Chick Retinas for Primary Cell Culture Studies: An Ex-ovo Electroporation Approach

M. Natalia Vergara¹, Christian Gutierrez¹, M. Valeria Canto-Soler¹

¹Wilmer Eye Institute, The Johns Hopkins University School of Medicine

JoVE 52002

Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements

Nabil E. Boutagy^1,2, George W. Rogers³, Emily S. Pyne¹, Mostafa M. Ali¹, Matthew W. Hulver^1,2, Madlyn I. Frisard^1,2

¹The Department of Human Nutrition, Foods, and Exercise, Virginia Tech, ²The Metabolic Phenotyping Core, Virginia Tech, ³Seahorse Bioscience

JoVE 53216

Instrumentation of Near-term Fetal Sheep for Multivariate Chronic Non-anesthetized Recordings

Patrick Burns¹, Hai Lun Liu², Shikha Kuthiala², Gilles Fecteau¹, André Desrochers¹, Lucien Daniel Durosier², Mingju Cao², Martin G. Frasch^2,3,4

¹Département de sciences cliniques, CHUV, Université de Montréal, St-Hyacinthe, QC, ²Département d'obstetriques et de gynécologie, CHU Ste-Justine Research Centre, Université de Montréal, ³Département de neurosciences, CHU Ste-Justine Centre de recherche, Université de Montréal, ⁴Centre de recherche en reproduction animale (CRRA), Université de Montréal, St-Hyacinthe, QC

JoVE 52581

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