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JoVE Journal

Developmental Biology

JoVE Developmental Biology includes methods for the study of biological development at all levels, from cellular to whole organisms.

‹1…24 25 590 VIDEO ARTICLES

Capturing Tissue Repair in Zebrafish Larvae with Time-lapse Brightfield Stereomicroscopy

Thomas S. Lisse¹, Elizabeth A. Brochu¹, Sandra Rieger¹

¹Davis Center for Regenerative Biology and Medicine, MDI Biological Laboratory

Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells

Tongguang Wang¹, Elliot Choi¹, Maria Chiara G. Monaco², Eugene O. Major², Marie Medynets¹, Avindra Nath¹

¹Translational Neuroscience Center, National Institute of Neurological Disorders and Stroke, National Institutes of Health, ²Laboratory of Molecular Medicine and Neuroscience, National Institute of Neurological Disorders and Stroke, National Institutes of Health

Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion (FDAP)

Katherine W. Rogers¹, Alexander Bläßle², Alexander F. Schier¹, Patrick Müller²

¹Department of Molecular and Cellular Biology, Harvard University, ²Systems Biology of Development Group, Friedrich Miescher Laboratory of the Max Planck Society

Optimized Ex-ovo Culturing of Chick Embryos to Advanced Stages of Development

Kellie Cloney¹, Tamara Anne Franz-Odendaal¹

¹Department of Biology, Mount Saint Vincent University

Culture of Embryonic Mouse Cochlear Explants and Gene Transfer by Electroporation

Khujista D. Haque¹, Atul K. Pandey¹, Matthew W. Kelley², Chandrakala Puligilla¹

¹Department of Pathology and Laboratory Medicine, Medical University of South Carolina, College of Medicine, ²Laboratory of Cochlear Development, NIDCD, NIH

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis

Elizabeth R. Zielins*¹, Ruth Tevlin*¹, Michael S. Hu¹, Michael T. Chung¹, Adrian McArdle¹, Kevin J. Paik¹, David Atashroo¹, Christopher R. Duldulao¹, Anna Luan¹, Kshemendra Senarath-Yapa¹, Graham G. Walmsley¹, Taylor Wearda¹, Michael T. Longaker^1,2, Derrick C. Wan¹

¹Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Division of Plastic and Reconstructive Surgery, Stanford University School of Medicine, ²Institute for Stem Cell Biology and Regenerative Medicine, Stanford University

Production and Use of Lentivirus to Selectively Transduce Primary Oligodendrocyte Precursor Cells for In Vitro Myelination Assays

Haley M. Peckham¹, Anita H. Ferner¹, Lauren Giuffrida¹, Simon S. Murray^1,2, Junhua Xiao^1,2

¹Department of Anatomy and Neuroscience, The University of Melbourne, ²The Florey Institute of Neuroscience and Mental Health Research, The University of Melbourne

Live-imaging of the Drosophila Pupal Eye

Mark B. Hellerman¹, Richard H. Choe¹, Ruth I. Johnson¹

¹Biology Department, Wesleyan University

Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis

Verónica A. Lombardo*^1,2,3, Cécile Otten*^1,2, Salim Abdelilah-Seyfried^1,2,3

¹Max Delbrück Center for Molecular Medicine, ²Institute of Biochemistry and Biology, University of Potsdam, ³Institute of Molecular Biology, Medizinische Hochschule Hannover

Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle

Chibeza C. Agley^1,2, Anthea M. Rowlerson¹, Cristiana P. Velloso¹, Norman L. Lazarus¹, Stephen D. R. Harridge¹

¹Centre of Human and Aerospace Physiological Sciences, King's College London, ²Wellcome Trust-Medical Research Council, Cambridge Stem Cell Institute

Derivation of Cardiac Progenitor Cells from Embryonic Stem Cells

Ieng Lam Lei¹, Lei Bu², Zhong Wang¹

¹Cardiac Surgery, University of Michigan, ²Leon H Charney Division of Cardiology, New York University School of Medicine

Expression of Fluorescent Proteins in Branchiostoma lanceolatum by mRNA Injection into Unfertilized Oocytes

Estelle Hirsinger¹, João Emanuel Carvalho², Christine Chevalier^1,3, Georges Lutfalla⁴, Jean-François Nicolas¹, Nadine Peyriéras⁵, Michael Schubert²

¹Département de Biologie du Développement et Cellules Souches, Institut Pasteur, ²Laboratoire de Biologie du Développement de Villefranche-sur-Mer (UMR7009 CNRS/UPMC Univ Paris 06), Sorbonne Universités, ³Equipe Epigenetic Control of Normal and Pathological Hematopoiesis, Centre de Recherche en Cancérologie de Marseille, ⁴Unité de Dynamique des Interactions Membranaires Normales et Pathologiques, CNRS UMR5235/DAA/cc107/Université Montpellier II, ⁵Plateforme BioEmergences IBiSA FBI, CNRS-NED, Institut de Neurobiologie Alfred Fessard

Induction of Protein Deletion Through In Utero Electroporation to Define Deficits in Neuronal Migration in Transgenic Models

Devon S. Svoboda¹, Alysen Clark¹, David S. Park¹, Ruth S. Slack¹

¹Department of Cellular and Molecular Medicine, University of Ottawa

Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus

Steven J. Deimling¹, Rami R. Halabi^2,3, Stephanie A. Grover⁴, Jean H. Wang^2,5, Thomas A. Drysdale^2,4,5

¹Developmental and Stem Cell Biology, Hospital for Sick Children, ²Children's Health Research Institute, University of Western Ontario, ³Department of Physiology and Pharmacology, University of Western Ontario, ⁴Neurosciences and Mental Health, Hospital for Sick Children, ⁵Department of Paediatrics, University of Western Ontario

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