JoVE Journal

Developmental Biology

JoVE Developmental Biology includes methods for the study of biological development at all levels, from cellular to whole organisms.

‹1 2 3 4 5 6 7 8 9…25 › 577 VIDEO ARTICLES

Generation of a Human iPSC-Based Blood-Brain Barrier Chip

Srikanth Jagadeesan^1,2,3, Michael J. Workman⁴, Anna Herland^5,6, Clive N. Svendsen⁴, Gad D. Vatine^1,2,3

¹The Department of Physiology and Cell Biology, Faculty of Health Sciences, Ben-Gurion University of the Negev, ²The Regenerative Medicine and Stem Cell (RMSC) Research Center, Ben-Gurion University of the Negev, ³The Zlotowski Center for Neuroscience, Ben-Gurion University of the Negev, ⁴The Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, ⁵Division of Micro and Nanosystems, KTH Royal Institute of Technology, ⁶AIMES, Department of Neuroscience, Karolinska Institutet

JoVE 60925

Isolation, Culture, and Adipogenic Induction of Neural Crest Original Adipose-Derived Stem Cells from Periaortic Adipose Tissue

Yiding Qi*¹, Xiang Miao*², Lian Xu³, Mengxia Fu³, Shi Peng⁴, Kailei Shi⁵, Jun Li⁴, Maoqing Ye⁵, Ruogu Li¹

¹Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, ²Shanghai Institute of Nutrition and Health, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, ³Shanghai Key Laboratory of Hypertension, Shanghai Institute of Hypertension, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, ⁴Department of Cardiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, ⁵Department of Cardiology, Shanghai Key Laboratory of Clinical Geriatric Medicine, Huadong Hospital Affiliated to Fudan University

JoVE 60691

Microwaving and Fluorophore-Tyramide for Multiplex Immunostaining on Mouse Adrenals − Using Unconjugated Primary Antibodies from the Same Host Species

Qiongxia Lyu^1,2, Huifei Sophia Zheng², Karly Laprocina², Chen-Che Jeff Huang²

¹College of Animal Science & Technology, Henan University of Science and Technology, ²Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University

JoVE 60868

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

Eileen F. Ablondi¹, Sudip Paudel², Morgan Sehdev³, John P. Marken⁴, Andrew D. Halleran⁴, Atiqur Rahman⁵, Peter Kemper⁵, Margaret S. Saha²

¹Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Harvard University, ²Department of Biology, College of William and Mary, ³Harvard Medical School, Harvard University, ⁴Department of Bioengineering, California Institute of Technology, ⁵Department of Computer Science, College of William and Mary

JoVE 60726

Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage

Bipasha Bose*¹, Saketh Kapoor*¹, Utsav Sen*¹, Muhammad Nihad AS¹, Debajit Chaudhury¹, Sudheer Shenoy P¹

¹Stem Cells and Regenerative Medicine Centre, Yenepoya Research Centre, Yenepoya (Deemed to be University), Mangaluru-575018, Karnataka, India

JoVE 59924

A Co-Culture Method to Study Neurite Outgrowth in Response to Dental Pulp Paracrine Signals

Courtney Barkley¹, Rosa Serra¹, Sarah B. Peters¹

¹Cell, Developmental and Integrative Biology Department, University of Alabama at Birmingham

JoVE 60809

Development of a Larval Zebrafish Infection Model for Clostridioides difficile

Junkai Li¹, Can M. Ünal², Kazuhiko Namikawa¹, Michael Steinert^3,4,5, Reinhard W. Köster¹

¹Division of Cellular and Molecular Neurobiology, Zoological Institute, Technische Universität Braunschweig, ²Department of Molecular Biotechnology, Turkish-German University, ³Institut für Mikrobiologie, Technische Universität Braunschweig, ⁴Braunschweig Integrated Centre of Systems Biology, ⁵Helmholtz Centre for Infection Research

JoVE 60793

Quantification of Ethanol Levels in Zebrafish Embryos Using Head Space Gas Chromatography

C. Ben Lovely¹

¹Department of Biochemistry and Molecular Genetics, Alcohol Research Center, University of Louisville

JoVE 60766

Monocular Visual Deprivation and Ocular Dominance Plasticity Measurement in the Mouse Primary Visual Cortex

Ke Chen*^1,2, Yilei Zhao*¹, Ting Liu¹, Zhaohao Su¹, Huiliang Yu¹, Leanne Lai Hang Chan^3,4, Tiejun Liu^1,2, Dezhong Yao^1,2

¹The Clinical Hospital of Chengdu Brain Science Institute, MOE Key Lab for NeuroInformation, University of Electronic Science and Technology of China, ²Sichuan Institute for Brain Science and Brain-inspired Intelligence, ³Department of Electronic Engineering, City University of Hong Kong, ⁴Centre for Biosystems, Neuroscience, and Nanotechnology, City University of Hong Kong

JoVE 60600

Displacement Analysis of Myocardial Mechanical Deformation (DIAMOND) Reveals Segmental Heterogeneity of Cardiac Function in Embryonic Zebrafish

Junjie Chen¹, René R. Sevag Packard^1,2,3

¹Division of Cardiology, Department of Medicine, David Geffen School of Medicine at University of California, Los Angeles, ²Ronald Reagan UCLA Medical Center, ³Veterans Affairs West Los Angeles Medical Center

JoVE 60547

Quantifying Liver Size in Larval Zebrafish Using Brightfield Microscopy

Srishti Kotiyal*¹, Alexis Fulbright*¹, Liam K. O'Brien*¹, Kimberley J. Evason¹

¹Department of Pathology, Department of Oncological Sciences, and Huntsman Cancer Institute, University of Utah School of Medicine

JoVE 60744

Whole Mount Immunohistochemistry in Zebrafish Embryos and Larvae

Dena R. Hammond-Weinberger¹, Gary T. ZeRuth¹

¹Department of Biological Sciences, Murray State University

JoVE 60575

Genotyping and Quantification of In Situ Hybridization Staining in Zebrafish

Tomasz Dobrzycki*^1,2, Monika Krecsmarik*^1,2, Rui Monteiro^1,2,3

¹MRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford, ²BHF Centre of Research Excellence, ³Institute of Cancer and Genomic Sciences, University of Birmingham

JoVE 59956

Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells

Kilsoo Jeon¹, Kyeyoon Park², Anton M. Jetten¹

¹Immunity, Inflammation and Disease Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, ²NIH Stem Cell Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health

JoVE 60571

A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos

Sanat Upadhyay¹, Leoncio Vergara², Pranjali Shah², Jan-Åke Gustafsson^3,4, Ioannis Kakadiaris^1,4, Maria Bondesson⁵

¹Computational Biomedicine Lab, Texas Institute of Measurement Evaluation and Statistics, University of Houston, ²Institute of Biosciences and Technology, Texas A&M Health Science Center, ³Department of Biology and Biochemistry, Center for Nuclear Receptors and Cell Signaling, University of Houston, ⁴Department of Biosciences and Nutrition, Novum, Karolinska Institutet, ⁵Department of Intelligent Systems Engineering, Indiana University

JoVE 60321

Retrograde Tracing of Drosophila Embryonic Motor Neurons Using Lipophilic Fluorescent Dyes

Melissa Ana Inal¹, Kota Banzai¹, Daichi Kamiyama¹

¹Department of Cellular Biology, University of Georgia

JoVE 60716

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Wei Feng¹, Andrew Przysinda¹, Guang Li¹

¹Department of Developmental Biology, University of Pittsburgh School of Medicine

JoVE 60647

Analysis of Non-Human Primate Pancreatic Islet Oxygen Consumption

Joseph M. Elsakr¹, Charles Deeter², Valerie Ricciardi³, Maureen Gannon^1,4,5,6

¹Department of Molecular Physiology and Biophysics, Vanderbilt University, ²Agilent Technologies, ³Department of Biological Sciences, Vanderbilt University, ⁴Department of Veterans Affairs Tennessee Valley, ⁵Department of Medicine, Vanderbilt University Medical Center, ⁶Department of Cell and Developmental Biology, Vanderbilt University

JoVE 60696

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Meng Wu¹, Divya Kriti¹, Harm van Bakel^1,2, Ethylin Wang Jabs¹, Greg Holmes¹

¹Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, ²Icahn Institute for Data Science and Genomic Technology, Icahn School of Medicine at Mount Sinai

JoVE 60503

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Hiromi Shimojo¹, Ryoichiro Kageyama^1,2,3,4

¹Institute for Frontier Life and Medical Sciences, Kyoto University, ²Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University, ³Kyoto University Graduate School of Medicine, ⁴Kyoto University Graduate School of Biostudies

JoVE 60455

Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function

Robert Mahen^1,2, Reiner Schulte³

¹Photonics Group, Department of Physics, Imperial College London, ²The Medical Research Council Cancer Unit, Hutchison/MRC Research Centre, Cambridge Biomedical Campus, University of Cambridge, ³Cambridge Institute for Medical Research, Cambridge Biomedical Campus, University of Cambridge

JoVE 60550

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

Daniela Avila-González¹, Guadalupe García-López¹, Néstor E. Díaz-Martínez², Héctor Flores-Herrera³, Anayansi Molina-Hernández¹, Wendy Portillo⁴, Néstor F. Díaz¹

¹Departamento de Fisiología y Desarrollo Celular, Instituto Nacional de Perinatología, ²Biotecnología Médica y Farmacéutica CONACYT, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), ³Departamento de Inmunobioquímica, Instituto Nacional de Perinatología, ⁴Instituto de Neurobiología, UNAM

JoVE 60551

In Vitro Reconstitution of Spatial Cell Contact Patterns with Isolated Caenorhabditis elegans Embryo Blastomeres and Adhesive Polystyrene Beads

Christina Rou Hsu^1,2, Rain Xiong^1,2, Kenji Sugioka^1,2

¹Department of Zoology, The University of British Columbia, ²Life Sciences Institute, The University of British Columbia

JoVE 60422

Quantification of Self-renewal in Murine Mammosphere Cultures

Thalia Vlachou¹, Xieraili Aobuli¹, Errico D'Elia¹, Angela Santoro¹, Maria Cristina Moroni¹, Pier Giuseppe Pelicci^1,2

¹Department of Experimental Oncology, IEO, European Institute of Oncology IRCCS, ²Department of Oncology and Hemato-Oncology, University of Milan

JoVE 60256

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Immunology and Infection

Biology

Genetics

Neuroscience

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