JoVE Journal

Developmental Biology

JoVE Developmental Biology includes methods for the study of biological development at all levels, from cellular to whole organisms.

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Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton

Sandeep Gopal¹, Roger Pocock¹

¹Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Department of Anatomy and Developmental Biology, Monash University

JoVE 57702

Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans

Kunal Baxi¹, Carlos E. de Carvalho¹

¹Department of Biology, University of Saskatchewan

JoVE 57414

Culturing of Retinal Pigment Epithelial Cells on an Ex Vivo Model of Aged Human Bruch's Membrane

Hui Cai¹, Jie Gong¹, Lucian V. Del Priore¹, Tongalp H. Tezel², Mark A. Fields¹

¹Department of Ophthalmology and Visual Science, Yale School of Medicine, ²Edward S. Harkness Eye Institute, Columbia University School of Medicine

JoVE 57084

Whole-mount Clearing and Staining of Arabidopsis Flower Organs and Siliques

Afif Hedhly¹, Hannes Vogler¹, Christof Eichenberger¹, Ueli Grossniklaus¹

¹Department of Plant and Microbial Biology, Zurich-Basel Plant Science Center, University of Zurich

JoVE 56441

Three-dimensional Inflammatory Human Tissue Equivalents of Gingiva

Li Xiao¹, Hisashi Okamura², Yasuo Kumazawa²

¹Department of Pharmacology, The Nippon Dental University, ²Department of Oral and Maxillofacial Surgery, The Nippon Dental University Hospital

JoVE 57157

Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution

Tomoko Yamazaki^1,2, Wenling Li¹, Yoh-Suke Mukouyama¹

¹Laboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, ²Earle A. Chiles Research Institute, Robert W. Franz Cancer Center, Providence Portland Medical Center

JoVE 57406

Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks

George Merz¹, Valerie Schwenk¹, Ruchit Shah², Carolyn Salafia^1,2, Phillip Necaise², Michael Joyce¹, Tom Villani³, Michael Johnson³, Nick Crider³

¹The Institute for Basic Research, The New York State Office for People with Developmental Disabilities, ²Placental Analytics LLC, ³Visikol Inc.

JoVE 57099

Chemical Amputation and Regeneration of the Pharynx in the Planarian Schmidtea mediterranea

Divya A. Shiroor¹, Tisha E. Bohr¹, Carolyn E. Adler¹

¹Department of Molecular Medicine, College of Veterinary Medicine, Cornell University

JoVE 57168

Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes

Irene U. Wacker^1,2, Lisa Veith³, Waldemar Spomer^2,4, Andreas Hofmann^2,4, Marlene Thaler⁵, Stefan Hillmer⁶, Ulrich Gengenbach^2,4, Rasmus R. Schröder^1,2,3

¹Cryo Electron Microscopy, Centre for Advanced Materials, Universität Heidelberg, ²Heidelberg Karlsruhe Research Partnership (HEiKA), ³Cryo Electron Microscopy, BioQuant, Universitätsklinikum Heidelberg, ⁴Institute for Automation and Applied Computer Science, Karlsruhe Institute of Technology (KIT), ⁵Carl Zeiss Microscopy GmbH, ⁶Electron Microscopy Core Facility, Universität Heidelberg

JoVE 57059

Single-cell Photoconversion in Living Intact Zebrafish

Lauren Green^1,2, Cody J. Smith^1,2

¹Department of Biological Sciences, University of Notre Dame, ²Center for Stem Cells and Regenerative Medicine, University of Notre Dame

JoVE 57024

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation

Qinggang Dai*¹, Yujiao Han*², Furong Xie*¹, Xuhui Ma³, Zhan Xu², Xiao Liu¹, Weiguo Zou², Jun Wang¹

¹Department of Pediatric Dentistry, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Center of Stomatology, ²State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, ³Department of Oral and Maxillofacial-Head and Neck Oncology, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine

JoVE 56468

Live Cell Imaging of Chromosome Segregation During Mitosis

Prajakta Varadkar¹, Kazuyo Takeda², Brenton McCright¹

¹Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, US Food and Drug Administration, ²Microscopy and Imaging Core facility, Division of Viral Products, Center for Biologics Evaluation and Research, US Food and Drug Administration

JoVE 57389

Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa

David G. Valcarce^1,2, Vanesa Robles^2,3

¹Department of Molecular Biology, University of León, ²INDEGSAL, University of León, ³Planta de Cultivos el Bocal, Spanish Institute of Oceanography (IEO)

JoVE 55323

Long-term Behavioral and Reproductive Consequences of Embryonic Exposure to Low-dose Toxicants

Mahlet D. Mersha¹, Karla R. Sanchez², Murali K. Temburni², Harbinder S. Dhillon²

¹Department of Neurology, Johns Hopkins School of Medicine, ²Department of Biological Sciences, Delaware State University

JoVE 56771

A Neural Network-Based Identification of Developmentally Competent or Incompetent Mouse Fully-Grown Oocytes

Federica Cavalera¹, Mario Zanoni¹, Valeria Merico¹, Thi Thu Hien Bui^1,2, Martina Belli^1,3, Lorenzo Fassina⁴, Silvia Garagna¹, Maurizio Zuccotti¹

¹Laboratorio di Biologia dello Sviluppo, Dipartimento di Biologia e Biotecnologie "Lazzaro Spallanzani", University of Pavia, ²Institute of Biotechnology, University of Helsinki, ³Department of Reproductive Medicine, University of California San Diego, ⁴Dipartimento di Ingegneria Industriale e dell'Informazione, University of Pavia

JoVE 56668

Dissection and Staining of Drosophila Pupal Ovaries

Karen Sophia Park¹, Dorothea Godt², Daniel Kalderon¹

¹Department of Biological Sciences, Columbia University, ²Department of Cell and Systems Biology, University of Toronto

JoVE 56779

Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells (NPCs)

Madeline Williams*¹, Smrithi Prem*¹, Xiaofeng Zhou¹, Paul Matteson², Percy Luk Yeung³, Chi-Wei Lu³, Zhiping Pang⁴, Linda Brzustowicz⁵, James H. Millonig², Emanuel Dicicco-Bloom¹

¹Department of Neuroscience and Cell Biology, Rutgers Robert Wood Johnson Medical School, ²Center for Advanced Biotechnology and Medicine, Department of Neuroscience and Cell Biology, Rutgers Robert Wood Johnson Medical School, ³The Child Health Institute of NJ, Department of Obstetrics, Gynecology, and Reproductive Services, Rutgers Robert Wood Johnson Medical School, ⁴The Child Health Institute of NJ, Department of Neuroscience and Cell Biology, Rutgers Robert Wood Johnson Medical School, ⁵Department of Genetics, Rutgers University

JoVE 56628

Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients

Chiara Assunta Pilato*^1,2, Ilaria Stadiotti*¹, Angela Serena Maione¹, Valentina Saverio¹, Valentina Catto³, Fabrizio Tundo³, Antonio Dello Russo³, Claudio Tondo^2,3, Giulio Pompilio^1,2, Michela Casella*³, Elena Sommariva*¹

¹Vascular Biology and Regenerative Medicine Unit, Centro Cardiologico Monzino-IRCCS, ²Department of Clinical Sciences and Community Health, Università degli Studi di Milano, ³Heart Rhythm Center, Centro Cardiologico Monzino-IRCCS

JoVE 57263

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Polina Zjablovskaja*^1,2, Petr Danek*¹, Miroslava Kardosova¹, Meritxell Alberich-Jorda^1,2

¹Department of Hemato-Oncology, Institute of Molecular Genetics of the ASCR, ²Childhood Leukaemia Investigation Prague, Department of Paediatric Haematology and Oncology, 2nd Faculty of Medicine, Charles University

JoVE 57033

Following Endocardial Tissue Movements via Cell Photoconversion in the Zebrafish Embryo

Renee Wei-Yan Chow*^1,2,3,4, Paola Lamperti*^1,2,3,4, Emily Steed^1,2,3,4, Francesco Boselli^1,2,3,4, Julien Vermot^1,2,3,4

¹Institut de Génétique et de Biologie Moléculaire et Cellulaire, ²UMR7104, Centre National de la Recherche Scientifique, ³U964, Institut National de la Santé et de la Recherche Médicale, ⁴Université de Strasbourg

JoVE 57290

Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay

Salma H. Azam¹, Mitchell Smith², Vivek Somasundaram², Chad V. Pecot^2,3

¹Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, ²Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, ³Department of Medicine, University of North Carolina at Chapel Hill

JoVE 57309

Orthotopic Ovarian Transplantation Procedures to Investigate the Life- and Health-span Influence of Ovarian Senescence in Female Mice

Jeffrey B. Mason¹, Kate C. Parkinson¹, Tracy L. Habermehl¹

¹Department of Animal, Dairy and Veterinary Sciences, Center for Integrated BioSystems, School of Veterinary Medicine, Utah State University

JoVE 56638

In Vivo Imaging of Muscle-tendon Morphogenesis in Drosophila Pupae

Sandra B. Lemke¹, Frank Schnorrer^1,2

¹Muscle Dynamics Group, Max Planck Institute of Biochemistry, ²Aix Marseille University, CNRS, IBDM

JoVE 57312

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

Stephen R Wellard*¹, Jessica Hopkins*¹, Philip W. Jordan¹

¹Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health

JoVE 56453

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