JoVE Journal

Developmental Biology

JoVE Developmental Biology includes methods for the study of biological development at all levels, from cellular to whole organisms.

‹1 2 3 4 5 6 7 8 9…22 › 527 VIDEO ARTICLES

Isolation of Specific Neuron Populations from Roundworm Caenorhabditis elegans

Edward M. Germany¹, Nataliya Zahayko¹, Oleh Khalimonchuk^1,2,3,4

¹Department of Biochemistry, University of Nebraska, ²Nebraska Redox Biology Center, University of Nebraska, ³Nebraska Center for Integrated Biomolecular Communication, University of Nebraska, ⁴Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center

JoVE 60145

Human Blastocyst Biopsy and Vitrification

Roberta Maggiulli¹, Adriano Giancani^1,2, Danilo Cimadomo¹, Filippo M. Ubaldi¹, Laura Rienzi¹

¹G.EN.E.R.A. Centers for Reproductive Medicine, ²DAHFMO, Unit of Histology and Medical Embryology, Sapienza University of Rome

JoVE 59625

Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells

Balta Lucendo-Villarin¹, Hassan Rashidi^1,2, Sharmin Alhaque^1,3, Lena Fischer^1,4, Jose Meseguer-Ripolles¹, Yu Wang¹, Cliona O'Farrelly⁴, Michael Themis³, David C. Hay¹

¹MRC Centre for Regenerative Medicine, University of Edinburgh, ²UCL Great Ormond Street Institute of Child Health, University College London, ³Division of Biosciences, Department of Life Sciences, College of Health and Life Sciences, Brunel University London, ⁴School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin

JoVE 59965

X-ray Diffraction of Intact Murine Skeletal Muscle as a Tool for Studying the Structural Basis of Muscle Disease

Weikang Ma¹, Thomas C. Irving¹

¹BioCAT, Dept. of Biological Sciences, Illinois Institute of Technology

JoVE 59559

Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation

Danielle Sambo¹, Jingling Li¹, Thomas Brickler¹, Sundari Chetty^1,2

¹Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, ²Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine

JoVE 59833

Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Lovepreet Singh¹, Adam Schoen¹, Alexander Mahlandt¹, Bhavit Chhabra¹, James Steadham¹, Vijay Tiwari¹, Nidhi Rawat¹

¹Department of Plant Science and Landscape Architecture, University of Maryland, College Park

JoVE 59743

Use of Atomic Force Microscopy to Measure Mechanical Properties and Turgor Pressure of Plant Cells and Plant Tissues

Simone Bovio^1,2, Yuchen Long², Françoise Monéger²

¹SFR Biosciences, Université de Lyon, ²Laboratoire de Reproduction et Développement des Plantes, Université de Lyon, ENS de Lyon, UCBL, INRA, CNRS

JoVE 59674

Adult Mouse Digit Amputation and Regeneration: A Simple Model to Investigate Mammalian Blastema Formation and Intramembranous Ossification

Lindsay A. Dawson¹, Regina Brunauer¹, Katherine N. Zimmel¹, Osama Qureshi¹, Alyssa R. Falck², Patrick Kim³, Connor P. Dolan¹, Ling Yu¹, Yu-Lieh Lin¹, Benjamin Daniel¹, Mingquan Yan¹, Ken Muneoka¹

¹Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, ²Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, ³Department of Neurosurgery, University of Mississippi Medical Center

JoVE 59749

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies

Wei Zheng^1,2, Zhiguo Chen^1,2

¹Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, and Key Laboratory of Neurodegeneration, Ministry of Education, ²Center of Neural Injury and Repair, Beijing Institute for Brain Disorders

JoVE 59690

Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells

Orion Furmanski^1,2, Michael D. Nieves^1,2,3, Martin L. Doughty^1,2,3

¹Center for Neuroscience and Regenerative Medicine, Uniformed Services University, ²Department of Anatomy, Physiology, and Genetics, Uniformed Services University, ³Graduate Program in Neuroscience, Uniformed Services University

JoVE 59561

Live Imaging and Analysis of Muscle Contractions in Drosophila Embryo

Ishita Chandel¹, Ryan Baker¹, Naosuke Nakamura¹, Vlad Panin¹

¹Department of Biochemistry and Biophysics, Texas A&M University

JoVE 59404

In Vitro Generation of Heart Field-specific Cardiac Progenitor Cells

Emmanouil Tampakakis¹, Matthew Miyamoto¹, Chulan Kwon¹

¹Division of Cardiology, Department Medicine, Johns Hopkins School of Medicine

JoVE 59826

Methods to Test Endocrine Disruption in Drosophila melanogaster

Tiziana Francesca Bovier¹, Daniela Cavaliere¹, Michele Colombo¹, Gianfranco Peluso², Ennio Giordano³, Filomena Anna Digilio^1,2

¹Institute of Biosciences and BioResources, National Research Council of Italy (CNR), ²Institute of Research on Terrestrial Ecosystems (IRET), National Research Council of Italy (CNR), ³Department of Biology, UniNa "Federico II"

JoVE 59535

HUVEC Tube-formation Assay to Evaluate the Impact of Natural Products on Angiogenesis

Maria Teresa Gentile¹, Olga Pastorino¹, Maurizio Bifulco², Luca Colucci-D'Amato¹

¹Laboratory of Cellular and Molecular Neuropathology, Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", ²Department of Molecular Medicine and Biotechnologies, University of Naples "Federico II" Naples

JoVE 58591

Formation of Human Periodontal Ligament Cell Spheroids on Chitosan Films

Xiangzhen Yan*¹, Xing Ran*², Siying Xia¹, Yanan Yang¹, Min Zhou¹, Chunxue Yuan³, Lijun Luo¹

¹Department of Periodontology, School & Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, ²Department of Periodontology, Dental Diseases Prevention & Treatment Center of Jiading District, ³College of Materials Science and Engineering, Tongji University

JoVE 59855

Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition

Ryo Ohta*¹, Ryohichi Sugimura*¹, Akira Niwa¹, Megumu K. Saito¹

¹Center for iPS cell research and application (CiRA)

JoVE 59823

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

Kyle M. Loh^1,2, Amrita Palaria¹, Lay Teng Ang¹

¹Institute for Stem Cell Biology & Regenerative Medicine, Stanford-UC Berkeley Siebel Stem Cell Institute, Stanford University School of Medicine, ²Department of Developmental Biology, Stanford University School of Medicine

JoVE 58975

Fast and Efficient Expression of Multiple Proteins in Avian Embryos Using mRNA Electroporation

Martin Tran¹, Mohit Dave^2,3, Rusty Lansford^1,2,3

¹Department of Biological Sciences, University of Southern California, Los Angeles, ²Department of Radiology and Developmental Neuroscience Program, Saban Research Institute, Children's Hospital Los Angeles, ³Keck School of Medicine, Department of Radiology, University of Southern California, Los Angeles

JoVE 59664

Measuring Sperm Guidance and Motility within the Caenorhabditis elegans Hermaphrodite Reproductive Tract

Muhan Hu¹, Shara Legg¹, Michael A. Miller¹

¹Department of Cell, Developmental and Integrative Biology, University of Alabama at Birmingham

JoVE 59783

Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution

Leighton H. Duncan^1,5, Mark W. Moyle^1,5, Lin Shao^1,5, Titas Sengupta^1,5, Richard Ikegami^1,5, Abhishek Kumar^4,5, Min Guo^4,5, Ryan Christensen^4,5, Anthony Santella^2,5, Zhirong Bao^2,5, Hari Shroff^4,5, William Mohler^3,5, Daniel A. Colón-Ramos^1,5,6

¹Department of Neuroscience and Department of Cell Biology, Yale University School of Medicine, ²Developmental Biology Program, Sloan Kettering Institute, ³Department of Genetics and Genome Sciences and Center for Cell Analysis and Modeling, University of Connecticut Health Center, ⁴Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, ⁵WormGUIDES.org, ⁶Instituto de Neurobiología, Recinto de Ciencias Médicas, Universidad de Puerto Rico

JoVE 59533

Analysis of Actomyosin Dynamics at Local Cellular and Tissue Scales Using Time-lapse Movies of Cultured Drosophila Egg Chambers

Ivana Viktorinová^1,2, Robert Haase*^1,2, Tobias Pietzsch*^1,2, Ian Henry^1,2, Pavel Tomancak^1,2

¹Max Planck Institute of Molecular Cell Biology and Genetics, ²Center for Systems Biology Dresden

JoVE 58587

Ex Vivo Perfusion of the Rodent Placenta

Jeanine N. D'Errico¹, Sara B. Fournier², Phoebe A. Stapleton^1,2

¹Department of Pharmacology and Toxicology, Ernest Mario School of Pharmacy, Rutgers University, ²Environmental and Occupational Health Sciences Institute

JoVE 59412

A Mini-Invasive Internal Fixation Technique for Studying Immobilization-Induced Knee Flexion Contracture in Rats

Shihai Jiang*¹, Xiaoyou Yi*³, Yuansen Luo², Dongjie Yu¹, Yuangao Liu¹, Fei Zhang^1,4, Lei Zhu², Kun Wang¹

¹Department of Joint and Trauma Surgery, The Third Affiliated Hospital of Sun Yat-sen University, ²Department of Plastic Surgery, The Third Affiliated Hospital of Sun Yat-sen University, ³Department of Bone Surgery, Tungwah Hospital of Sun Yat-sen University, ⁴Department of Othopaedic Surgery, The Eighth Affiliated Hospital of Sun Yat-sen University

JoVE 59260

Minimally Invasive Embryo Transfer and Embryo Vitrification at the Optimal Embryo Stage in Rabbit Model

Ximo Garcia-Dominguez¹, Francisco Marco-Jimenez¹, Maria Pilar Viudes-de-Castro², Jose Salvador Vicente¹

¹Departament of Animal Science, Institute for Animal Science and Technology (ICTA), Universitat Politècnica de València (UPV), ²Animal Technology and Research Center (CITA), Instituto Valenciano de Investigaciones Agrarias (IVIA)

JoVE 58055

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Neuroscience

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