JoVE Journal

Developmental Biology

JoVE Developmental Biology covers research studies of biological development at all levels, ranging from cellular to whole organisms.

‹1…3 4 5 6 7 8 9…15 › 358 VIDEO ARTICLES

Using In Vivo and Tissue and Cell Explant Approaches to Study the Morphogenesis and Pathogenesis of the Embryonic and Perinatal Aorta

Ashish Misra¹, Zhonghui Feng¹, Jiasheng Zhang¹, Zhi-Yin Lou^2,3, Daniel M. Greif¹

¹Yale Cardiovascular Research Center, Section of Cardiovascular Medicine, Department of Internal Medicine, Yale University School of Medicine, ²Department of Neurology, Yale University School of Medicine, ³Department of Neurology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine

JoVE 56039

In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana

Daisuke Kurihara*^1,2, Yusuke Kimata*¹, Tetsuya Higashiyama^1,2,3, Minako Ueda^1,3

¹Division of Biological Science, Graduate School of Science, Nagoya University, ²Higashiyama Live-Holonics Project, JST-ERATO, Nagoya University, ³Institute of Transformative Bio-Molecules (ITbM), Nagoya University

JoVE 55975

Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies

Roman Crazzolara¹, Gabriele Kropshofer¹, Michael Steurer², Sieghart Sopper^2,3, Wolfgang Schwinger⁴

¹Department of Pediatrics, Medical University Innsbruck, ²Department of Internal Medicine V (Hematology & Oncology), Medical University Innsbruck, ³Tyrolean Cancer Research Institute, ⁴Department of Pediatrics, Medical University Graz

JoVE 56002

Shifting Zebrafish Lethal Skeletal Mutant Penetrance by Progeny Testing

Elliott P. Brooks¹, James T. Nichols¹

¹Department of Craniofacial Biology, University of Colorado Anschutz Medical Campus

JoVE 56200

Bottlenose Dolphin (Tursiops truncatus) Spermatozoa: Collection, Cryopreservation, and Heterologous In Vitro Fertilization

María Jesús Sánchez-Calabuig^1,2, Francisco Alberto García-Vázquez³, Ricardo Laguna-Barraza¹, Carlos Barros-García⁴, Daniel García-Parraga⁵, Dimitrios Rizos¹, Alfonso Gutiérrez Adan¹, José Félix Pérez-Gutíerrez²

¹Department of Animal Reproduction, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), ²Department of Animal Medicine and Surgery, School of Veterinary Medicine, Universidad Complutense de Madrid, ³Department of Physiology, Faculty of Veterinary Science, University of Murcia, Campus Mare Nostrum, ⁴Mundomar, Benidorm, ⁵Veterinary Services, L'Oceanográfic, Ciudad de las Artes y las Ciencias, Junta de Murs i Vals, s/n, 46013

JoVE 55237

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

Marta Vila-Cejudo¹, Elena Ibañez¹, Josep Santalo¹

¹Unitat de Biologia Cellular, Facultat de Biociències, Universitat Autònoma de Barcelona

JoVE 56171

Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos

Antionette L. Williams¹, Brenda L. Bohnsack¹

¹Department of Ophthalmology and Visual Sciences, Kellogg Eye Center, University of Michigan

JoVE 56214

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells

Peter Szaraz*^1,2, Yarden S. Gratch*¹, Farwah Iqbal^1,2, Clifford L. Librach^1,2,3,4,5

¹Create Fertility Centre, ²Department of Physiology, University of Toronto, ³Department of Obstetrics and Gynecology, University of Toronto, ⁴Department of Physiology, University of Toronto, ⁵Department of Obstetrics and Gynecology, Women's College Hospital

JoVE 55757

A Method for Characterizing Embryogenesis in Arabidopsis

Jinlin Feng^1,2, Ligeng Ma¹

¹College of Life Sciences, Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, Capital Normal University, ²College of Life Science, Shanxi Normal University

JoVE 55969

A Novel Mammary Fat Pad Transplantation Technique to Visualize the Vessel Generation of Vascular Endothelial Stem Cells

Qing Cissy Yu¹, Wenqian Song¹, Dengwen Lai¹, Yi Arial Zeng¹

¹State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences

JoVE 55795

Whole-cell Patch-clamp Recordings of Isolated Primary Epithelial Cells from the Epididymis

Bao Li Zhang^1,2,3, Da Yuan Gao^1,2,3, Xiao Xu Zhang^1,3, Shuo Shi⁴, Winnie Shum¹

¹School of Life Science and Technology, ShanghaiTech University, ²Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, ³University of Chinese Academy of Sciences, ⁴Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University

JoVE 55700

Horizontal Whole Mount: A Novel Processing and Imaging Protocol for Thick, Three-dimensional Tissue Cross-sections of Skin

Lucia Salz^1,2, Ryan R. Driskell^1,2

¹Centre for Stem Cells and Regenerative Medicine, King's College London, ²School of Molecular Biosciences, Washington State University

JoVE 56106

Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging

Diana Passaro*¹, Ander Abarrategi*¹, Katie Foster¹, Linda Ariza-McNaughton¹, Dominique Bonnet¹

¹Haematopoietic Stem Cell Laboratory, The Francis Crick Institute

JoVE 55914

Live Imaging of Mouse Secondary Palate Fusion

Seungil Kim¹, Jan Prochazka², Jeffrey O. Bush¹

¹Department of Cell and Tissue Biology, Program in Craniofacial Biology and Institute for Human Genetics, University of California San Francisco, ²Laboratory of Transgenic Models Diseases, Czech Centre for Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences

JoVE 56041

A Standardized Method for Measuring Internal Lung Surface Area via Mouse Pneumonectomy and Prosthesis Implantation

Zhe Liu^1,2, Siling Fu^2,3, Nan Tang²

¹Department of Life Sciences, Peking University, ²National Institute of Biological Sciences, Beijing, ³Graduate School of Peking Union Medical College

JoVE 56114

Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement

Thomas Laumonier*¹, Stéphane Koenig*², Sophie Saüc^2,3, Maud Frieden³

¹Department of Orthopedic Surgery, Geneva University Hospitals & Faculty of Medicine, ²Department of Basic Neurosciences, Geneva Medical Center, ³Department of Cell Physiology and Metabolism, Geneva Medical Center

JoVE 55918

Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells

Yueying Li², Yong Hai¹, Jiayu Chen³, Tie Liu¹

¹Department of Orthopedics, Beijing Chao-Yang Hospital, Capital Medical University, ²Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, ³Clinical and Translational Research Center of Shanghai First Maternity & Infant Hospital, School of Life Sciences and Technology, Tongji University

JoVE 55722

Identification Of Erythromyeloid Progenitors And Their Progeny In The Mouse Embryo By Flow Cytometry

Lorea Iturri^1,2, Javier Saenz Coronilla¹, Yvan Lallemand¹, Elisa Gomez Perdiguero¹

¹Department of Developmental and Stem Cell Biology, CNRS UMR3738, Department of Immunology, Institut Pasteur, ²Cellule Pasteur UPMC, University Pierre et Marie Curie

JoVE 55305

A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry

Ana C. Lima*^1,2,3,4, Min Jung*¹, Jannette Rusch¹, Abul Usmani¹, Alexandra M. Lopes^3,4, Donald F. Conrad¹

¹Department of Genetics, Washington University School of Medicine, ²Graduate Program in Areas of Basic and Applied Biology (GABBA), Abel Salazar Institute of Biomedical Sciences, University of Porto, ³Instituto de Investigação e Inovação em Saúde, University of Porto, ⁴IPATIMUP - Instituto de Patologia e Imunologia Molecular da Universidade do Porto, ⁵Department of Pathology & Immunology, Washington University School of Medicine

JoVE 55913

Quantifying Branching Density in Rat Mammary Gland Whole-mounts Using the Sholl Analysis Method

Jason P. Stanko¹, Suzanne E. Fenton¹

¹National Toxicology Program Laboratory, Division of the National Toxicology Program, National Institute of Environmental Health Sciences

JoVE 55789

A Modified Trier Social Stress Test for Vulnerable Mexican American Adolescents

Megan M. Johnson¹, Julianna Deardorff¹, Kimberly Parra¹, Abbey Alkon², Brenda Eskenazi¹, Elizabeth Shirtcliff³

¹Center for Environmental Research and Children's Health (CERCH), Berkley School of Public Health, University of California, Berkeley, ²San Francisco (UCSF) School of Nursing, University of California, San Francisco, ³Human Development and Family Studies, Iowa State University

JoVE 55393

Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix

Elias Uhlin¹, Ana Marin Navarro^1,2, Harriet Rönnholm¹, Kelly Day¹, Malin Kele¹, Anna Falk¹

¹Department of Neuroscience, Karolinska Institutet, ²Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet

JoVE 56146

High-resolution Episcopic Microscopy (HREM) - Simple and Robust Protocols for Processing and Visualizing Organic Materials

Stefan H. Geyer¹, Barbara Maurer-Gesek¹, Lukas F. Reissig¹, Wolfgang J. Weninger¹

¹Division of Anatomy, Center for Anatomy and Cell Biology & MIC, Medical University of Vienna

JoVE 56071

Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting

Kyan Thelen¹, Nadia Ayala-Lopez², Stephanie W. Watts², G. Andres Contreras¹

¹Department of Large Animal Clinical Sciences, Michigan State University, ²Department of Pharmacology and Toxicology, Michigan State University

JoVE 55818

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Immunology and Infection

Biology

Genetics

Neuroscience

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