JoVE Journal

Developmental Biology

JoVE Developmental Biology includes methods for the study of biological development at all levels, from cellular to whole organisms.

‹1…3 4 5 6 7 8 9…25 › 578 VIDEO ARTICLES

Quantification of Self-renewal in Murine Mammosphere Cultures

Thalia Vlachou¹, Xieraili Aobuli¹, Errico D'Elia¹, Angela Santoro¹, Maria Cristina Moroni¹, Pier Giuseppe Pelicci^1,2

¹Department of Experimental Oncology, IEO, European Institute of Oncology IRCCS, ²Department of Oncology and Hemato-Oncology, University of Milan

JoVE 60256

Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids

Tânia Martins Garcia¹, Marit Navis¹, Manon E. Wildenberg¹, Ruurd M. van Elburg², Vanesa Muncan¹

¹Department of Gastroenterology and Hepatology, Tytgat Institute for Intestinal and Liver Research, Amsterdam UMC, AG&M, University of Amsterdam, ²Department of Pediatrics, Amsterdam UMC, University of Amsterdam

JoVE 60470

Analysis of Hematopoietic Stem Progenitor Cell Metabolism

Giorgia Scapin^1,2,3, Marie C. Goulard^1,2,3, Priyanka R. Dharampuriya^1,2,3, Jennifer L. Cillis^1,2,3, Dhvanit I. Shah^1,2,3

¹Nationwide Children's Hospital, ²The Ohio State University College of Medicine, ³The Ohio State University Comprehensive Cancer Center

JoVE 60234

Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors

Rita Silvério-Alves^1,2,3, Andreia M. Gomes³, Ilia Kurochkin⁴, Kateri A. Moore^5,6, Carlos-Filipe Pereira^1,2,3

¹Molecular Medicine and Gene Therapy, Lund Stem Cell Center, Lund University, ²Wallenberg Center for Molecular, Lund University, ³Center for Neuroscience and Cell Biology, University of Coimbra, ⁴Skolkovo Institute of Science and Technology, ⁵Department of Cell, Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, ⁶Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai

JoVE 60112

Imaging Dpp Release from a Drosophila Wing Disc

Laura Faith George¹, Emily Anne Bates¹

¹Department of Pediatrics, University of Colorado School of Medicine

JoVE 60528

A Semi-high-throughput Imaging Method and Data Visualization Toolkit to Analyze C. elegans Embryonic Development

Renat N. Khaliullin^1,2,3, Jeffrey M. Hendel^1,2, Adina Gerson-Gurwitz^1,2, Shaohe Wang^1,4,5, Stacy D. Ochoa^1,6, Zhiling Zhao^1,7, Arshad Desai^1,2, Karen Oegema^1,2, Rebecca A. Green^1,2

¹Ludwig Institute for Cancer Research, San Diego, ²Department of Cellular and Molecular Medicine, University of California, San Diego, ³Recursion Pharmaceuticals, ⁴Biomedical Sciences Graduate Program, University of California, San Diego, ⁵Cell Biology Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, ⁶Department of Biology, San Diego State University, ⁷Developmental and Stem Cell Biology Graduate Program, University of California, San Francisco

JoVE 60362

Clonal Genetic Tracing using the Confetti Mouse to Study Mineralized Tissues

Baoyi Zhou¹, Marketa Kaucka¹, Andrei S. Chagin^1,2, Phillip T. Newton^1,3

¹Department of Physiology and Pharmacology, Karolinska Institutet, ²Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), ³Department of Women's and Children's Health, Karolinska Institutet and Pediatric Endocrinology Unit, Karolinska University Hospital

JoVE 60424

An Enhanced Green Fluorescence Protein-based Assay for Studying Neurite Outgrowth in Primary Neurons

Wai Wa Ray Chan*¹, Wen Li*^1,2, Dik Long Dennis Chau*¹, Kwok-Fai Lau¹

¹School of Life Sciences, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, ²Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen

JoVE 60031

Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants

Megan Vaughan¹, Katja A. Lamia¹

¹Scripps Research, Department of Molecular Medicine

JoVE 60310

Generation of Multicellular Human Primary Endometrial Organoids

Alina R. Murphy*¹, Teerawat Wiwatpanit*², Zhenxiao Lu¹, Batzaya Davaadelger¹, J. Julie Kim¹

¹Department of Obstetrics and Gynecology, Northwestern University, ²National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA)

JoVE 60384

Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration

Sandra Guadalupe Gonzalez Malagon*^1,2, Lisa Dobson*^1,3, Anna M Lopez Muñoz¹, Marcus Dawson¹, William Barrell^1,3, Petros Marangos^2,4, Matthias Krause³, Karen J Liu¹

¹Centre for Craniofacial and Regenerative Biology, King's College London, ²Institute of Molecular Biology and Biotechnology, FORTH, Department of Biomedical Research, University of Ioannina, ³Randall Centre of Cell & Molecular Biophysics, King's College London, ⁴Department of Biological Applications and Technology, University of Ioannina

JoVE 60051

An Ectopic Chemokine Expression Model for Testing Macrophage Recruitment In Vivo

Yunyun Jiang¹, Jiahao Chen¹, Jin Xu²

¹Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, ²Division of Cell, Developmental and Integrative Biology, School of Medicine, South China University of Technology

JoVE 60161

Assay for Blood-brain Barrier Integrity in Drosophila melanogaster

Matthew J. Davis¹, Danielle Talbot¹, Jennifer Jemc¹

¹Department of Biology, Loyola University Chicago

JoVE 60233

Three-dimensional Angiogenesis Assay System using Co-culture Spheroids Formed by Endothelial Colony Forming Cells and Mesenchymal Stem Cells

Sajita Shah*¹, Hyunsook Lee*¹, Young Hwan Park¹, Eunkyeong Jeon¹, Hye Kyung Chung², Eun Sang Lee², Jae Hoon Shim², Kyu-Tae Kang¹

¹College of Pharmacy, Duksung Innovative Drug Center, Duksung Women's University, ²Korea Drug Development Platform using Radio-isotope, Korea Institute of Radiological & Medical Sciences

JoVE 60032

Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment

Matteo Da Ros*¹, Tiina Lehtiniemi*¹, Opeyemi Olotu¹, Oliver Meikar¹, Noora Kotaja¹

¹Institute of Biomedicine, University of Turku

JoVE 60271

Performing Human Skeletal Muscle Xenografts in Immunodeficient Mice

Kyla A. Britson^1,2, Aaron D. Black^2,3, Kathryn R. Wagner^1,2,3,4, Thomas E. Lloyd^1,2,4

¹Graduate Program in Cellular and Molecular Medicine, Johns Hopkins University School of Medicine, ²Department of Neurology, Johns Hopkins University School of Medicine, ³Kennedy Krieger Institute, ⁴Department of Neuroscience, Johns Hopkins University School of Medicine

JoVE 59966

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

Kinga Winczura¹, Pawel Grzechnik¹

¹School of Biosciences, University of Birmingham

JoVE 59700

CRISPR/Cas9 Technology in Restoring Dystrophin Expression in iPSC-Derived Muscle Progenitors

Yue Jin¹, Yan Shen¹, Xuan Su¹, Neal Weintraub¹, Yaoliang Tang¹

¹Medical College of Georgia, Augusta University

JoVE 59432

Methods Collection

Electroporation Method for In Vivo Delivery of Plasmid DNA in the Adult Zebrafish Telencephalon

Tamara Durovic^1,2, Jovica Ninkovic^1,3,4

¹Institute of Stem Cell Research, Helmholtz Zentrum München, ²Graduate School of Systemic Neurosciences, Ludwig Maximilian University, ³Department of Cell Biology and Anatomy, Biomedical Center, Ludwig Maximilian University, ⁴Munich Cluster for Systems Neurology (SyNergy)

JoVE 60066

Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles

Sophia Dao¹, Kenton Jones¹, Tamira Elul¹

¹College of Osteopathic Medicine, Touro University California

JoVE 60123

Evaluation of Fertilization State by Tracing Sperm Nuclear Morphology in Arabidopsis Double Fertilization

Taro Takahashi¹, Tomoko Igawa¹

¹Graduate School of Horticulture, Chiba University

JoVE 59916

A Direct and Simple Method to Assess Drosophila melanogaster's Viability from Embryo to Adult

Antonio L. Rockwell¹, Isabel Beaver¹, Cintia F. Hongay¹

¹Department of Biology, Clarkson University

JoVE 59996

Isolation, Culture, Characterization, and Differentiation of Human Muscle Progenitor Cells from the Skeletal Muscle Biopsy Procedure

Brandon J. Gheller*¹, Jamie Blum*¹, Sharon Soueid-Baumgarten², Erica Bender¹, Benjamin D. Cosgrove², Anna Thalacker-Mercer¹

¹Division of Nutritional Sciences, Cornell University, ²Meinig School of Biomedical Engineering, Cornell University

JoVE 59580

Injecting Gryllus bimaculatus Eggs

Samantha K. Barry¹, Taro Nakamura², Yuji Matsuoka³, Christoph Straub⁴, Hadley W. Horch⁴, Cassandra G. Extavour⁵

¹Colby College, ²Division of Evolutionary Developmental Biology, National Institute for Basic Biology, ³Department of Biological Sciences, National University of Singapore, ⁴Department Biology and Department of Neuroscience, Bowdoin College, ⁵Department of Organismic and Evolutionary Biology and Department of Molecular and Cellular Biology, Harvard University

JoVE 59726

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Immunology and Infection

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