JoVE Journal

Developmental Biology

JoVE Developmental Biology includes methods for the study of biological development at all levels, from cellular to whole organisms.

‹1…3 4 5 6 7 8 9…22 › 527 VIDEO ARTICLES

Genotyping of Sea Anemone during Early Development

Miguel A.P. Silva¹, Nagayasu Nakanishi¹

¹Department of Biological Sciences, University of Arkansas

JoVE 59541

An In Vitro 3D Model and Computational Pipeline to Quantify the Vasculogenic Potential of iPSC-Derived Endothelial Progenitors

Cody O. Crosby¹, Janet Zoldan¹

¹Department of Biomedical Engineering, University of Texas at Austin

JoVE 59342

Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone

Jingwen Yang*^1,2, Haichun Pan*², Yuji Mishina²

¹The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine of Ministry of Education, School and Hospital of Stomatology, Wuhan University, ²Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan

JoVE 59113

Genetic Engineering of Primary Mouse Intestinal Organoids Using Magnetic Nanoparticle Transduction Viral Vectors for Frozen Sectioning

Lingling Xian¹, Lionel Chia^1,5, Dan Georgess², Li Luo¹, Shuai Shuai¹, Andrew J. Ewald^3,4, Linda M.S. Resar^1,4,5,6

¹Department of Medicine, Division of Hematology, Johns Hopkins University School of Medicine, ²Department of Natural Sciences, School of Arts & Sciences, Lebanese American University, ³Department of Cell Biology, Johns Hopkins University School of Medicine, ⁴Department of Oncology, Johns Hopkins University School of Medicine, ⁵Department of Pathology, Johns Hopkins University School of Medicine, ⁶Institute for Cell Engineering, Johns Hopkins University School of Medicine

JoVE 57040

Isolation and Staining of Mouse Skin Keratinocytes for Cell Cycle Specific Analysis of Cellular Protein Expression by Mass Cytometry

Josiah Fernandez¹, Enrique C Torchia¹

¹Department of Dermatology and Charles C. Gates Center for Regenerative Medicine, University of Colorado Anschutz Medical Campus

JoVE 59353

Surgical Size Reduction of Zebrafish for the Study of Embryonic Pattern Scaling

Kana Ishimatsu¹, Anna Cha¹, Zach M. Collins¹, Sean G. Megason¹

¹Department of Systems Biology, Harvard Medical School

JoVE 59434

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture

Shawna M. Rotoli¹, Salvatore J. Caradonna¹

¹Department of Molecular Biology, Rowan University, School of Osteopathic Medicine and Graduate School of Biomedical Sciences

JoVE 59483

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging

Darren Wisniewski¹, Sally Lowell¹, Guillaume Blin¹

¹MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh

JoVE 59634

Determining Bile Duct Density in the Mouse Liver

Joshua M. Adams^1,2,3, Hamed Jafar-Nejad^1,3

¹Program in Developmental Biology, Baylor College of Medicine, ²Medical Scientist Training Program (MSTP), Baylor College of Medicine, ³Department of Molecular and Human Genetics, Baylor College of Medicine

JoVE 59587

Neurogenesis Using P19 Embryonal Carcinoma Cells

Paweł Leszczyński¹, Magdalena Śmiech¹, Aamir S. Teeli¹, Aleksandra Zołocińska², Anna Słysz², Zygmunt Pojda², Mariusz Pierzchała³, Hiroaki Taniguchi¹

¹Department of Experimental Embryology, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, ²Department of Regenerative Medicine, Maria Skłodowska-Curie Institute - Oncology Center, ³Department of Genomics and Biodiversities, Institute of Genetics and Animal Breeding, Polish Academy of Sciences

JoVE 58225

Culturing and Measuring Fetal and Newborn Murine Long Bones

Veronica Uribe¹, Alberto Rosello-Diez¹

¹Australian Regenerative Medicine Institute, Monash University

JoVE 59509

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

Taiki Nakajima¹, Hidetoshi Sakurai², Makoto Ikeya²

¹Department of Life Science Frontiers, Center for iPS Cells Research and Application, Kyoto University, ²Department of Clinical Application, Center for iPS Cells Research and Application, Kyoto University

JoVE 59359

Generation of Organoids from Mouse Extrahepatic Bile Ducts

Junya Shiota¹, Nureen H. Mohamad Zaki¹, Juanita L. Merchant^1,2, Linda C. Samuelson^1,2, Nataliya Razumilava¹

¹Departments of Internal Medicine, The University of Michigan, ²Molecular & Integrative Physiology, The University of Michigan

JoVE 59544

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells

Yena Kim^1,2, Ji Hyeon Ju^1,2,3

¹CiSTEM Laboratory, Catholic Induced Pluripotent Stem Cell (iPSC) Research Center, College of Medicine, The Catholic University of Korea, ²Department of Biomedicine & Health Science, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, ³Division of Rheumatology, Department of Internal Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea

JoVE 59297

Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence

Zhentao Zhang^1,2,3, Young-Jae Nam^1,2,3

¹Department of Medicine, Division of Cardiovascular Medicine, Vanderbilt University Medical Center, ²Department of Cell and Developmental Biology, Vanderbilt University, ³Vanderbilt Center for Stem Cell Biology, Vanderbilt University

JoVE 59413

Thawing, Culturing, and Cryopreserving Drosophila Cell Lines

Arthur Luhur¹, Kristin M. Klueg¹, Johnny Roberts¹, Andrew C. Zelhof¹

¹Drosophila Genomics Resource Center, Department of Biology, Indiana University Bloomington

JoVE 59459

Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells

Hannah C. Leeson^1,2, Tailoi Chan-Ling^3,4, Michael D. Lovelace^3,5,6, Jeremy D. Brownlie⁷, Michael W. Weible II*^1,4,7, Ben J. Gu*⁸

¹Griffith Institute for Drug Discovery, Griffith University, ²Australian Institute for Bioengineering and Nanotechnology, University of Queensland, ³Discipline of Anatomy and Histology, School of Medical Science, University of Sydney, ⁴Bosch Institute, University of Sydney, ⁵Applied Neurosciences Program, Peter Duncan Neurosciences Research Unit, St. Vincent's Centre for Applied Medical Research, ⁶School of Medical Sciences, The University of New South Wales (UNSW) Medicine, Sydney, New South Wales, ⁷School of Environment and Science, Griffith University, Brisbane, Queensland, ⁸Florey Institute of Neuroscience and Mental Health, University of Melbourne

JoVE 59313

Confocal Live Imaging of Shoot Apical Meristems from Different Plant Species

Yuan Geng^1,2, Yun Zhou^1,2

¹Department of Botany and Plant Pathology, Purdue University, ²Purdue Center for Plant Biology, Purdue University

JoVE 59369

Electroretinogram Recording in Larval Zebrafish using A Novel Cone-Shaped Sponge-tip Electrode

Jiaheng Xie¹, Patricia R. Jusuf¹, Patrick T. Goodbourn², Bang V. Bui³

¹School of Biosciences, University of Melbourne, ²Melbourne School of Psychological Sciences, University of Melbourne, ³Department of Optometry and Vision Sciences, University of Melbourne

JoVE 59487

Visualization of the Superior Ocular Sulcus during Danio rerio Embryogenesis

Kevin H. Yoon¹, Sonya A. Widen^1,2, Melissa M. Wilson¹, Jennifer C. Hocking^2,3,4,5, Andrew J. Waskiewicz^1,2,6

¹Department of Biological Sciences, University of Alberta, ²Women & Children's Health Research Institute, University of Alberta, ³Division of Anatomy, Department of Surgery, University of Alberta, ⁴Department of Cell Biology, University of Alberta, ⁵Department of Medical Genetics, University of Alberta, ⁶Neuroscience and Mental Health Research Institute, University of Alberta

JoVE 59259

Efficient Generation of Pancreas/Duodenum Homeobox Protein 1⁺ Posterior Foregut/Pancreatic Progenitors from hPSCs in Adhesion Cultures

Taro Toyoda¹, Azuma Kimura¹, Hiromi Tanaka¹, Kenji Osafune¹

¹Center for iPS Cell Research and Application (CiRA), Kyoto University

JoVE 57641

Studying Oxidative Stress Caused by the Mitis Group Streptococci in Caenorhabditis elegans

Ali Naji¹, Ali Al Hatem¹, Ransome van der Hoeven¹

¹Department of Diagnostic and Biomedical Sciences, School of Dentistry, University of Texas Health Science Center

JoVE 59301

Combining Histochemical Staining and Image Analysis to Quantify Starch in the Ovary Primordia of Sweet Cherry during Winter Dormancy

Erica Fadon¹, Javier Rodrigo¹

¹Instituto Agroalimentario de Aragón - IA2 (CITA-Universidad de Zaragoza), Centro de Investigación y Tecnología Agroalimentaria de Aragón

JoVE 58524

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells

Stefano Martellucci^1,2, Costantino Santacroce¹, Valeria Manganelli², Francesca Santilli^1,2, Luca Piccoli³, Michele Cassetta³, Roberta Misasi², Maurizio Sorice², Vincenzo Mattei^1,2

¹Laboratory of Experimental Medicine and Environmental Pathology, Sabina Universitas - Rieti University Hub, ²Department of Experimental Medicine, Sapienza University of Rome, ³Department of Science Dentistry and Maxillofacial, Sapienza University of Rome

JoVE 59282

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