JoVE Journal

Developmental Biology

JoVE Developmental Biology includes methods for the study of biological development at all levels, from cellular to whole organisms.

‹1…3 4 5 6 7 8 9…21 › 498 VIDEO ARTICLES

Isolation of Embryonic Tissues and Formation of Quail-Chicken Chimeric Organs Using The Thymus Example

Marta Figueiredo¹, Hélia Neves¹

¹Instituto de Histologia e Biologia do Desenvolvimento, Faculdade de Medicina, Universidade de Lisboa

JoVE 58965

Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants

Sota Iwatani¹, Makiko Yoshida², Keiji Yamana¹, Daisuke Kurokawa¹, Jumpei Kuroda¹, Khin Kyae Mon Thwin¹, Suguru Uemura¹, Satoru Takafuji¹, Nanako Nino¹, Tsubasa Koda, Masami Mizobuchi⁴, Masahiro Nishiyama¹, Kazumichi Fujioka¹, Hiroaki Nagase¹, Ichiro Morioka⁵, Kazumoto Iijima¹, Noriyuki Nishimura¹

¹Department of Pediatrics, Kobe University Graduate School of Medicine, ²Department of Pathology, Kobe Children's Hospital, ³Department of Pediatrics, Hyogo College of Medicine, ⁴Department of Developmental Pediatrics, Shizuoka Children's Hospital, ⁵Department of Pediatrics, Nihon University School of Medicine

JoVE 58806

Visualizing Cellular Gibberellin Levels Using the nlsGPS1 Förster Resonance Energy Transfer (FRET) Biosensor

Annalisa Rizza¹, Ankit Walia¹, Bijun Tang¹, Alexander M. Jones¹

¹Sainsbury Laboratory, University of Cambridge

JoVE 58739

Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells

Shino Mitsunaga¹, Keiko Shioda¹, Kurt J. Isselbacher¹, Jacob H. Hanna², Toshi Shioda¹

¹Center for Cancer Research, Massachusetts General Hospital, ²Department of Molecular Genetics, Weizmann Institute of Science

JoVE 58297

An In Vitro Model of a Parallel-Plate Perfusion System to Study Bacterial Adherence to Graft Tissues

Bartosz Ditkowski¹, Tiago R Veloso¹, Martyna Bezulska-Ditkowska^1,2, Andreas Lubig³, Stefan Jockenhoevel³, Petra Mela³, Ramadan Jashari⁴, Marc Gewillig¹, Bart Meyns⁵, Marc F Hoylaerts², Ruth Heying¹

¹Cardiovascular Developmental Biology, Department of Cardiovascular Sciences, KU Leuven, ²Centre for Molecular and Vascular Biology, Department of Cardiovascular Sciences, KU Leuven, ³Department of Biohybrid & Medical Textiles, AME - Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, ⁴European Homograft Bank, Saint Jean Clinique, ⁵Division of Clinical Cardiac Surgery, Department of Cardiovascular Sciences, KU Leuven

JoVE 58476

Induction of Endothelial Differentiation in Cardiac Progenitor Cells Under Low Serum Conditions

Michika Mochizuki*¹, Giacomo Della Verde*¹, Habiba Soliman¹, Otmar Pfister^1,2, Gabriela M. Kuster^1,2

¹Department of Biomedicine, University Hospital and University of Basel, ²Division of Cardiology, University Hospital Basel

JoVE 58370

Derivation and Differentiation of Canine Ovarian Mesenchymal Stem Cells

Amanda B.T. Hill^1,2, Jonathan E.B.T. Hill¹, Fabiana F. Bressan³, Maria Angélica Miglino⁴, Joaquim M. Garcia¹

¹The School of Agricultural and Veterinary Studies (FCAV), Jaboticabal, São Paulo State University (UNESP), ²CRRF, Université de Montréal, ³FZEA, University of São Paulo, ⁴FMVZ, University of São Paulo

JoVE 58163

Direct Lineage Reprogramming of Adult Mouse Fibroblast to Erythroid Progenitors

Melissa Ilsley¹, Sandra Capellera-Garcia¹, Kishori Dhulipala¹, Alban Johansson¹, Johan Flygare¹

¹Department of Molecular Medicine and Gene Therapy, Lund University

JoVE 58464

An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity

Mengrou Liu*¹, Chunsen Zhu*¹, Shun Bai*², Xin Li¹, Kaiqiang Fu¹, Lan Ye¹, Ke Zheng¹

¹State Key Laboratory of Reproductive Medicine, Nanjing Medical University, ²Center for Reproductive Medicine, Department of Obstetrics and Gynecology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China

JoVE 58512

Fluorimetric Techniques for the Assessment of Sperm Membranes

Alisa Komsky-Elbaz^1,2, Zvi Roth^1,2

¹Animal Sperm Research Center, Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, ²Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem

JoVE 58622

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

Patrick S. McGrath^1,2,3, Nicole Diette^1,2, Igor Kogut^1,2,3, Ganna Bilousova^1,2,3

¹Department of Dermatology, University of Colorado School of Medicine, Anschutz Medical Campus, ²Charles C. Gates Center for Regenerative Medicine, University of Colorado School of Medicine, Anschutz Medical Campus, ³Stem Cell Biobank and Disease Modeling Core, University of Colorado School of Medicine, Anschutz Medical Campus

JoVE 58687

Microfluidic Assay for the Assessment of Leukocyte Adhesion to Human Induced Pluripotent Stem Cell-derived Endothelial Cells (hiPSC-ECs)

Oleh V. Halaidych¹, Francijna van den Hil¹, Christine L. Mummery^1,2, Valeria V. Orlova¹

¹Department of Anatomy and Embryology, Leiden University Medical Center, ²Department of Applied Stem Cell Technologies, University of Twente

JoVE 58678

A Mouse Distraction Osteogenesis Model

Masahito Fujio¹, Yusuke Osawa², Masaki Matsushita², Kota Ogisu¹, Shuhei Tsuchiya¹, Hiroshi Kitoh², Hideharu Hibi¹

¹Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, ²Department of Orthopaedic Surgery, Nagoya University Graduate School of Medicine

JoVE 57925

High Frequency Ultrasound for the Analysis of Fetal and Placental Development In Vivo

Nicole Meyer¹, Thomas Schüler², Ana Claudia Zenclussen¹

¹Experimental Obstetrics and Gynecology, Medical Faculty, Otto-von-Guericke University, ²Institute of Molecular and Clinical Immunology, Medical Faculty, Otto-von-Guericke University

JoVE 58616

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence

Cally Xiao^1,2,3, Frank Nitsche⁴, Hisham Bazzi^2,3

¹Graduate Program in Pharmacology and Experimental Therapeutics, University of Cologne, ²Department of Dermatology and Venereology, University Hospital of Cologne, ³Cologne Cluster of Excellence in Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, ⁴Department of General Ecology, Institute for Zoology, Biocenter Cologne, University of Cologne

JoVE 58321

Probing Cell Mechanics with Bead-Free Optical Tweezers in the Drosophila Embryo

Claire Chardès*¹, Raphael Clement*¹, Olivier Blanc¹, Pierre-François Lenne¹

¹CNRS, Institut de Biologie du Développement de Marseille, Aix-Marseille Université

JoVE 57900

Visualize Drosophila Leg Motor Neuron Axons Through the Adult Cuticle

Wenyue Guan¹, Lalanti Venkatasubramanian², Myungin Baek³, Richard S. Mann², Jonathan Enriquez¹

¹Institut de Génomique Fonctionnelle de Lyon, ENS de Lyon, CNRS, ²Departments of Biochemistry and Molecular Biophysics, and Neuroscience, Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University, ³Neuroscience Program, NYU School of Medicine

JoVE 58365

Visualization of Tangential Cell Migration in the Developing Chick Optic Tectum

Yuji Watanabe¹, Chie Sakuma¹, Hiroyuki Yaginuma¹

¹Department of Neuroanatomy and Embryology, School of Medicine, Fukushima Medical University

JoVE 58506

Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells

Heidi Hongisto*¹, Meri Vattulainen*¹, Tanja Ilmarinen¹, Alexandra Mikhailova², Heli Skottman¹

¹BioMediTech Institute, Faculty of Medicine and Life Sciences, University of Tampere, ²Department of Ophthalmology, SILK, Faculty of Medicine and Life Sciences, University of Tampere

JoVE 58279

Cell Cycle Analysis in the C. elegans Germline with the Thymidine Analog EdU

Zuzana Kocsisova^1,2, Ariz Mohammad², Kerry Kornfeld¹, Tim Schedl²

¹Department of Developmental Biology, Washington University School of Medicine, St. Louis, Missouri, ²Department of Genetics, Washington University School of Medicine, St. Louis, Missouri

JoVE 58339

Micromanipulation of Chromosomes in Insect Spermatocytes

Nicolas K.H. Lin¹, Ryder Nance², Jane Szybist², Alan Cheville², Leocadia V. Paliulis³

¹Program in Cell Biology/Biochemistry, Bucknell University, ²Department of Electrical and Computer Engineering, Bucknell University, ³Biology Department, Bucknell University

JoVE 57359

Adult Zebrafish Injury Models to Study the Effects of Prednisolone in Regenerating Bone Tissue

Karina Geurtzen¹, Franziska Knopf^1,2

¹CRTD – Center for Regenerative Therapies Dresden, TU Dresden, ²Center for Healthy Aging, TU Dresden

JoVE 58429

Intravenous and Intra-amniotic In Utero Transplantation in the Murine Model

Nicholas J. Ahn¹, John D. Stratigis¹, Barbara E. Coons¹, Alan W. Flake¹, Hyun-Duck Nah-Cederquist², William H. Peranteau¹

¹Division of General, Thoracic, and Fetal Surgery, Center for Fetal Research, Children's Hospital of Philadelphia, ²Division of Plastic Reconstructive Surgery, Center for Fetal Research, Children's Hospital of Philadelphia

JoVE 58047

Use of Hematopoietic Stem Cell Transplantation to Assess the Origin of Myelodysplastic Syndrome

Yang Jo Chung¹, Ghanwa Khawaja¹, Karen M. Wolcott², Peter D. Aplan¹

¹Genetics Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, ²LGI Flow Cytometry Core Facility, Center for Cancer Research, National Cancer Institute, National Institutes of Health

JoVE 58140

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