JoVE Journal

Developmental Biology

JoVE Developmental Biology includes methods for the study of biological development at all levels, from cellular to whole organisms.

‹1…3 4 5 6 7 8 9…18 › 414 VIDEO ARTICLES

A Neural Network-Based Identification of Developmentally Competent or Incompetent Mouse Fully-Grown Oocytes

Federica Cavalera¹, Mario Zanoni¹, Valeria Merico¹, Thi Thu Hien Bui^1,2, Martina Belli^1,3, Lorenzo Fassina⁴, Silvia Garagna¹, Maurizio Zuccotti¹

¹Laboratorio di Biologia dello Sviluppo, Dipartimento di Biologia e Biotecnologie "Lazzaro Spallanzani", University of Pavia, ²Institute of Biotechnology, University of Helsinki, ³Department of Reproductive Medicine, University of California San Diego, ⁴Dipartimento di Ingegneria Industriale e dell'Informazione, University of Pavia

JoVE 56668

Dissection and Staining of Drosophila Pupal Ovaries

Karen Sophia Park¹, Dorothea Godt², Daniel Kalderon¹

¹Department of Biological Sciences, Columbia University, ²Department of Cell and Systems Biology, University of Toronto

JoVE 56779

Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells (NPCs)

Madeline Williams*¹, Smrithi Prem*¹, Xiaofeng Zhou¹, Paul Matteson², Percy Luk Yeung³, Chi-Wei Lu³, Zhiping Pang⁴, Linda Brzustowicz⁵, James H. Millonig², Emanuel Dicicco-Bloom¹

¹Department of Neuroscience and Cell Biology, Rutgers Robert Wood Johnson Medical School, ²Center for Advanced Biotechnology and Medicine, Department of Neuroscience and Cell Biology, Rutgers Robert Wood Johnson Medical School, ³The Child Health Institute of NJ, Department of Obstetrics, Gynecology, and Reproductive Services, Rutgers Robert Wood Johnson Medical School, ⁴The Child Health Institute of NJ, Department of Neuroscience and Cell Biology, Rutgers Robert Wood Johnson Medical School, ⁵Department of Genetics, Rutgers University

JoVE 56628

Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients

Chiara Assunta Pilato*^1,2, Ilaria Stadiotti*¹, Angela Serena Maione¹, Valentina Saverio¹, Valentina Catto³, Fabrizio Tundo³, Antonio Dello Russo³, Claudio Tondo^2,3, Giulio Pompilio^1,2, Michela Casella*³, Elena Sommariva*¹

¹Vascular Biology and Regenerative Medicine Unit, Centro Cardiologico Monzino-IRCCS, ²Department of Clinical Sciences and Community Health, Università degli Studi di Milano, ³Heart Rhythm Center, Centro Cardiologico Monzino-IRCCS

JoVE 57263

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Polina Zjablovskaja*^1,2, Petr Danek*¹, Miroslava Kardosova¹, Meritxell Alberich-Jorda^1,2

¹Department of Hemato-Oncology, Institute of Molecular Genetics of the ASCR, ²Childhood Leukaemia Investigation Prague, Department of Paediatric Haematology and Oncology, 2nd Faculty of Medicine, Charles University

JoVE 57033

Following Endocardial Tissue Movements via Cell Photoconversion in the Zebrafish Embryo

Renee Wei-Yan Chow*^1,2,3,4, Paola Lamperti*^1,2,3,4, Emily Steed^1,2,3,4, Francesco Boselli^1,2,3,4, Julien Vermot^1,2,3,4

¹Institut de Génétique et de Biologie Moléculaire et Cellulaire, ²UMR7104, Centre National de la Recherche Scientifique, ³U964, Institut National de la Santé et de la Recherche Médicale, ⁴Université de Strasbourg

JoVE 57290

Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay

Salma H. Azam¹, Mitchell Smith², Vivek Somasundaram², Chad V. Pecot^2,3

¹Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, ²Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, ³Department of Medicine, University of North Carolina at Chapel Hill

JoVE 57309

Orthotopic Ovarian Transplantation Procedures to Investigate the Life- and Health-span Influence of Ovarian Senescence in Female Mice

Jeffrey B. Mason¹, Kate C. Parkinson¹, Tracy L. Habermehl¹

¹Department of Animal, Dairy and Veterinary Sciences, Center for Integrated BioSystems, School of Veterinary Medicine, Utah State University

JoVE 56638

In Vivo Imaging of Muscle-tendon Morphogenesis in Drosophila Pupae

Sandra B. Lemke¹, Frank Schnorrer^1,2

¹Muscle Dynamics Group, Max Planck Institute of Biochemistry, ²Aix Marseille University, CNRS, IBDM

JoVE 57312

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

Stephen R Wellard*¹, Jessica Hopkins*¹, Philip W. Jordan¹

¹Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health

JoVE 56453

Zika Virus Infection of Cultured Human Fetal Brain Neural Stem Cells for Immunocytochemical Analysis

Erica L. McGrath^1,2, Junling Gao¹, Ping Wu^1,3

¹Department of Neuroscience and Cell Biology, University of Texas Medical Branch, ²Department of Neurology, Johns Hopkins University, ³Beijing Institute for Brain Disorders, Capital Medical University

JoVE 56917

Single-Cell Quantification of Protein Degradation Rates by Time-Lapse Fluorescence Microscopy in Adherent Cell Culture

Andrea Brigitta Alber¹, David Michael Suter¹

¹UPSUTER, Institute of Bioengineering (IBI), School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL)

JoVE 56604

Kinetic Analysis of Vasculogenesis Quantifies Dynamics of Vasculogenesis and Angiogenesis In Vitro

Kaela M. Varberg^1,2, Seth Winfree^3,4, Kenneth W. Dunn^3,4, Laura S. Haneline^1,2,5,6,7

JoVE 57044

Methods for Analyzing the Impacts of Natural Uranium on In Vitro Osteoclastogenesis

Tatiana Gritsaenko¹, Valérie Pierrefite-Carle¹, Gaëlle Creff², Claude Vidaud³, Georges Carle¹, Sabine Santucci-Darmanin¹

¹UMR E-4320 TIRO-MATOs CEA/DRF/BIAM, Université Nice Sophia-Antipolis, ²UMR 7272 Institut de Chimie de Nice CNRS, Université Nice Sophia-Antipolis, ³CEA, Direction de la Recherche Fondamentale (DRF), Biosciences and Biotechnologies Institute (BIAM)

JoVE 56499

Handling and Treatment of Male European Eels (Anguilla anguilla) for Hormonal Maturation and Sperm Cryopreservation

Juan G. Herranz-Jusdado¹, Eszter Kása², Tímea Kollár², Víctor Gallego¹, David S. Peñaranda¹, Christoffer Rozenfeld¹, Luz Pérez¹, Ákos Horváth², Juan F. Asturiano¹

¹Grupo de Acuicultura y Biodiversidad, Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, ²Department of Aquaculture, Institute of Aquaculture and Environmental Safety, Szent Istvan University

JoVE 56835

Application of Chronic Stimulation to Study Contractile Activity-induced Rat Skeletal Muscle Phenotypic Adaptations

Yuho Kim^1,2,3, Jonathan M. Memme^1,2, David A. Hood^1,2

¹Muscle Health Research Centre, York University, ²School of Kinesiology and Health Science, York University, ³National Heart, Lung, and Blood Institute, National Institutes of Health

JoVE 56827

Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea

Rabab Sharif¹, Shrestha Priyadarsini², Tyler G. Rowsey², Jian-Xing Ma³, Dimitrios Karamichos^1,2

¹Department of Cell Biology, University of Oklahoma Health Sciences Center, ²Department of Ophthalmology/Dean McGee Eye Institute, University of Oklahoma Health Sciences Center, ³Department of Physiology, Harold Hamm Diabetes Center, University of Oklahoma Health Sciences Center

JoVE 56308

Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells

Olivia Krefft*¹, Ammar Jabali*¹, Vira Iefremova¹, Philipp Koch^1,2,3, Julia Ladewig¹

¹Institute of Reconstructive Neurobiology, University of Bonn, ²Central Institute of Mental Health, University of Heidelberg/Medical Faculty Mannheim, ³Hector Institute for Translational Brain Research (HITBR gGmbH)

JoVE 56768

Mass Isolation and In Vitro Cultivation of Intramolluscan Stages of the Human Blood Fluke Schistosoma Mansoni

Nathalie Dinguirard¹, Codie Heinemann¹, Timothy P. Yoshino¹

¹Department of Pathobiological Sciences, University of Wisconsin, School of Veterinary Medicine

JoVE 56345

Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice

David E. Maridas¹, Elizabeth Rendina-Ruedy¹, Phuong T. Le¹, Clifford J. Rosen¹

¹Maine Medical Center Research Institute

JoVE 56750

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

Ashutosh Pandey¹, Hamed Jafar-Nejad^1,2

¹Department of Molecular and Human Genetics, Baylor College of Medicine, ²Program in Developmental Biology, Baylor College of Medicine

JoVE 56919

Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans

Ena Kolundzic¹, Stefanie Seelk¹, Baris Tursun¹

¹Berlin Institute for Medical Systems Biology, Max Delbrück Center for Molecular Medicine in the Helmholtz Association

JoVE 56889

A Rapid and Efficient Method to Dissect Pupal Wings of Drosophila Suitable for Immunodetections or PCR Assays

Carmen Bolatto¹, Cristina Parada¹, Victoria Colmenares¹

¹Departamento de Histología y Embriología, Facultad de Medicina, Laboratorio de Biología del Desarrollo

JoVE 55854

Generation and Culturing of Primary Human Keratinocytes from Adult Skin

Claus Johansen¹

¹Department of Dermatology, Aarhus University Hospital

JoVE 56863

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RELATED SECTIONS

Immunology and Infection

Biology

Genetics

Neuroscience

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