JoVE Journal

Developmental Biology

JoVE Developmental Biology includes methods for the study of biological development at all levels, from cellular to whole organisms.

‹1…6 7 8 9 10 11…16 › 377 VIDEO ARTICLES

Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies

Junning Yang¹, Chuanshen Wu², Ioana Stefanescu¹, Arie Horowitz^1,3

¹Department of Medicine, Cardeza Vascular Research Center, Sidney Kimmel Medical College, Thomas Jefferson University, ²Department of Molecular Cardiology, Cleveland Clinic Foundation, ³Department of Cancer Biology, Cardeza Vascular Research Center, Sidney Kimmel Medical College, Thomas Jefferson University

JoVE 55621

Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish

Elaine L. Welch*¹, Celeste C. Eno*¹, Sreelaja Nair², Robin E. Lindeman³, Francisco Pelegri¹

¹Laboratory of Genetics, University of Wisconsin-Madison, ²Department of Biological Sciences, Tata Institute of Fundamental Research, ³Department of Genetics, Cell Biology, and Development, University of Minnesota

JoVE 55213

Surgical Ablation Assay for Studying Eye Regeneration in Planarians

Jacob M. Morton*¹, Marwa A. Saad*¹, Wendy S. Beane¹

¹Department of Biological Sciences, Western Michigan University

JoVE 55594

Protocols for Visualizing Steroidogenic Organs and Their Interactive Organs with Immunostaining in the Fruit Fly Drosophila melanogaster

Eisuke Imura*¹, Yuto Yoshinari*¹, Yuko Shimada-Niwa*², Ryusuke Niwa³

¹Graduate School of Life and Environmental Sciences, University of Tsukuba, ²Life Science Center of Tsukuba Advanced Research Alliance, University of Tsukuba, ³Faculty of Life and Environmental Sciences, University of Tsukuba

JoVE 55519

Generation of iPSC-derived Human Brain Organoids to Model Early Neurodevelopmental Disorders

Elke Gabriel¹, Jay Gopalakrishnan^1,2

¹Center for Molecular Medicine Cologne, University of Cologne, ²Institute for Biochemistry I, Medical School of University of Cologne

JoVE 55372

Using Primary Neurosphere Cultures to Study Primary Cilia

Issei S. Shimada¹, Hemant Badgandi¹, Bandarigoda N. Somatilaka¹, Saikat Mukhopadhyay¹

¹Department of Cell Biology, University of Texas Southwestern Medical Center

JoVE 55315

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Kathryn E. Ware¹, Shivee Gilja¹, Shenghan Xu¹, Samantha Shetler¹, Mohit K. Jolly², Xueyang Wang³, Suzanne Bartholf Dewitt⁴, Alexander J. Hish¹, Sarah Jordan¹, William Eward⁵, Herbert Levine², Andrew J. Armstrong⁴, Jason A. Somarelli¹

¹Department of Medicine, Duke University, ²Department of Bioengineering, Rice University, ³Department of Molecular Genetics and Microbiology, Duke University, ⁴Solid Tumor Program and the Duke Prostate Center, Duke University Medical Center, ⁵Duke University Medical Center

JoVE 55520

Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta

Naimisha Beeravolu^1,2, Christina McKee^1,2, Ali Alamri^1,2, Sasha Mikhael³, Christina Brown^1,2, Mick Perez-Cruet^2,4, G. Rasul Chaudhry^1,2

¹Department of Biological Sciences, Oakland University, ²OU-WB Institute for Stem Cell and Regenerative Medicine, ³Department of Obstetrics and Gynecology, St. John Provindence - Providence Park Hospital, ⁴Department of Neurosurgery, Beaumont Health System

JoVE 55224

Live Confocal Imaging of Developing Arabidopsis Flowers

Nathanaël Prunet¹

¹Department of Biology and Biological Engineering, California Institute of Technology

JoVE 55156

In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences

Stefanie Dudczig^1,2, Peter D. Currie², Lucia Poggi³, Patricia R. Jusuf^1,2

¹School of Biosciences, The University of Melbourne, ²Australian Regenerative Medicine Institute (ARMI), Monash University, ³The David J Apple Center for Vision Research, Department of Ophthalmology, Heidelberg University

JoVE 55490

Assessing Cardiomyocyte Subtypes Following Transcription Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts

Antonio Fernandez-Perez¹, Nikhil V. Munshi¹

¹Department of Internal Medicine- Cardiology, UT Southwestern Medical Center

JoVE 55456

Conditional Reprogramming of Pediatric Human Esophageal Epithelial Cells for Use in Tissue Engineering and Disease Investigation

Todd J. Jensen¹, Christopher Foster¹, Wael Sayej², Christine M. Finck³

¹Department of Pediatrics, UConn Health, ²Department of Gastroenterology, Connecticut Children's Medical Center, ³Department of Surgery, Connecticut Children's Medical Center

JoVE 55243

Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches

Lin Wang*¹, Malgorzata M. Kamocka*², Amy Zollman³, Nadia Carlesso³

¹Wells Center for Pediatric Research, Department of Pediatrics, Indiana University School of Medicine, ²Indiana Center for Biological Microscopy, Department of Medicine, Indiana University School of Medicine, ³Department of Pediatrics, Indiana University School of Medicine

JoVE 54253

Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions

Joni H. Ylostalo¹, Nikolay Bazhanov², Arezoo Mohammadipoor³, Thomas J. Bartosh⁴

¹Department of Biology, University of Mary Hardin-Baylor, ²Department of Pediatrics, University of Texas Medical Branch, ³Multi-Organ Support Technology Task Area, U.S. Army Institute of Surgical Research, ⁴Internal Medicine, Texas A&M University Health Science Center

JoVE 55126

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

Jianle Wang¹, Montserrat C. Anguera¹

¹Department of Biomedical Sciences, School of Veterinary Medicine, University of Pennsylvania

JoVE 55268

A Device for Performing Cell Migration/Wound Healing in a 96-Well Plate

Po Ying Poon¹, Patrick Ying Kit Yue¹, Ricky Ngok Shun Wong¹

¹Department of Biology, Hong Kong Baptist University

JoVE 55411

Efficient Differentiation of Pluripotent Stem Cells to NKX6-1⁺ Pancreatic Progenitors

Emily C. McGaugh^1,2,3, M. Cristina Nostro^1,2,3

¹Toronto General Research Institute, University Health Network, ²McEwen Centre for Regenerative Medicine, University Health Network, ³Department of Physiology, University of Toronto

JoVE 55265

Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells

Yu Wang¹, Sharmin Alhaque¹, Kate Cameron¹, Jose Meseguer-Ripolles¹, Baltasar Lucendo-Villarin¹, Hassan Rashidi¹, David C. Hay¹

¹MRC Centre for Regenerative Medicine, University of Edinburgh

JoVE 55355

Cell Lineage Analyses and Gene Function Studies Using Twin-spot MARCM

Hung-Chang Shen^1,2, Tsai-Chi Hsu¹, Pei-Chi Chung¹, Hung-Hsiang Yu^1,2

¹Institute of Cellular and Organismic Biology, Academia Sinica, ²Graduate Institute of Life Sciences, National Defense Medical Center

JoVE 55278

Isolation of Mouse Endometrial Epithelial and Stromal Cells for In Vitro Decidualization

Katrien De Clercq*¹, Aurélie Hennes*¹, Joris Vriens¹

¹Department of Development and Regeneration, Katholieke Universiteit Leuven (KU Leuven)

JoVE 55168

Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts

Mitchel C. Schiewe¹, Shane Zozula¹, Nancy Nugent¹, Kelley Waggoner¹, Jessica Borba¹, Lisa Gamboa¹, John B. Whitney¹

¹Ovation Fertility

JoVE 54871

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Gurpreet Kaur^1,2, Jaimo Ahn¹, Kurt D. Hankenson^1,3, Jason W. Ashley^1,4

¹Department of Orthopaedic Surgery, Perelman School of Medicine, University of Pennsylvania, ²Department of Biological Sciences, University of the Sciences, ³The Department of Small Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, ⁴Department of Biology, College of Science, Technology, Engineering, & Mathematics, Eastern Washington University

JoVE 55234

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

Enrica Bertin¹, Martina Piccoli¹, Chiara Franzin¹, Andras Nagy², Maria Mileikovsky², Paolo De Coppi³, Michela Pozzobon¹

¹Stem Cell and Regenerative Medicine Laboratory, Fondazione Istituto di Ricerca Pediatrica Citta della Speranza, ²Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, ³Stem Cells and Regenerative Medicine Section, Developmental Biology and Cancer Programme, UCL Institute of Child Health and Great Ormond Street Hospital

JoVE 54598

Culture of Adult Transgenic Zebrafish Retinal Explants for Live-cell Imaging by Multiphoton Microscopy

Manuela Lahne¹, Ryne A. Gorsuch¹, Craig M. Nelson^1,2, David R. Hyde¹

¹Department of Biological Sciences, University of Notre Dame, ²Department of Neurosurgery, Mayo Clinic

JoVE 55335

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Immunology and Infection

Biology

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