JoVE Journal

Developmental Biology

JoVE Developmental Biology includes methods for the study of biological development at all levels, from cellular to whole organisms.

‹1…6 7 8 9 10 11…18 › 418 VIDEO ARTICLES

Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging

Diana Passaro*¹, Ander Abarrategi*¹, Katie Foster¹, Linda Ariza-McNaughton¹, Dominique Bonnet¹

¹Haematopoietic Stem Cell Laboratory, The Francis Crick Institute

JoVE 55914

Live Imaging of Mouse Secondary Palate Fusion

Seungil Kim¹, Jan Prochazka², Jeffrey O. Bush¹

¹Department of Cell and Tissue Biology, Program in Craniofacial Biology and Institute for Human Genetics, University of California San Francisco, ²Laboratory of Transgenic Models Diseases, Czech Centre for Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences

JoVE 56041

A Standardized Method for Measuring Internal Lung Surface Area via Mouse Pneumonectomy and Prosthesis Implantation

Zhe Liu^1,2, Siling Fu^2,3, Nan Tang²

¹Department of Life Sciences, Peking University, ²National Institute of Biological Sciences, Beijing, ³Graduate School of Peking Union Medical College

JoVE 56114

Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement

Thomas Laumonier*¹, Stéphane Koenig*², Sophie Saüc^2,3, Maud Frieden³

¹Department of Orthopedic Surgery, Geneva University Hospitals & Faculty of Medicine, ²Department of Basic Neurosciences, Geneva Medical Center, ³Department of Cell Physiology and Metabolism, Geneva Medical Center

JoVE 55918

Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells

Yueying Li², Yong Hai¹, Jiayu Chen³, Tie Liu¹

¹Department of Orthopedics, Beijing Chao-Yang Hospital, Capital Medical University, ²Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, ³Clinical and Translational Research Center of Shanghai First Maternity & Infant Hospital, School of Life Sciences and Technology, Tongji University

JoVE 55722

Identification Of Erythromyeloid Progenitors And Their Progeny In The Mouse Embryo By Flow Cytometry

Lorea Iturri^1,2, Javier Saenz Coronilla¹, Yvan Lallemand¹, Elisa Gomez Perdiguero¹

¹Department of Developmental and Stem Cell Biology, CNRS UMR3738, Department of Immunology, Institut Pasteur, ²Cellule Pasteur UPMC, University Pierre et Marie Curie

JoVE 55305

A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry

Ana C. Lima*^1,2,3,4, Min Jung*¹, Jannette Rusch¹, Abul Usmani¹, Alexandra M. Lopes^3,4, Donald F. Conrad¹

¹Department of Genetics, Washington University School of Medicine, ²Graduate Program in Areas of Basic and Applied Biology (GABBA), Abel Salazar Institute of Biomedical Sciences, University of Porto, ³Instituto de Investigação e Inovação em Saúde, University of Porto, ⁴IPATIMUP - Instituto de Patologia e Imunologia Molecular da Universidade do Porto, ⁵Department of Pathology & Immunology, Washington University School of Medicine

JoVE 55913

Quantifying Branching Density in Rat Mammary Gland Whole-mounts Using the Sholl Analysis Method

Jason P. Stanko¹, Suzanne E. Fenton¹

¹National Toxicology Program Laboratory, Division of the National Toxicology Program, National Institute of Environmental Health Sciences

JoVE 55789

A Modified Trier Social Stress Test for Vulnerable Mexican American Adolescents

Megan M. Johnson¹, Julianna Deardorff¹, Kimberly Parra¹, Abbey Alkon², Brenda Eskenazi¹, Elizabeth Shirtcliff³

¹Center for Environmental Research and Children's Health (CERCH), Berkley School of Public Health, University of California, Berkeley, ²San Francisco (UCSF) School of Nursing, University of California, San Francisco, ³Human Development and Family Studies, Iowa State University

JoVE 55393

Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix

Elias Uhlin¹, Ana Marin Navarro^1,2, Harriet Rönnholm¹, Kelly Day¹, Malin Kele¹, Anna Falk¹

¹Department of Neuroscience, Karolinska Institutet, ²Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet

JoVE 56146

High-resolution Episcopic Microscopy (HREM) - Simple and Robust Protocols for Processing and Visualizing Organic Materials

Stefan H. Geyer¹, Barbara Maurer-Gesek¹, Lukas F. Reissig¹, Wolfgang J. Weninger¹

¹Division of Anatomy, Center for Anatomy and Cell Biology & MIC, Medical University of Vienna

JoVE 56071

Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting

Kyan Thelen¹, Nadia Ayala-Lopez², Stephanie W. Watts², G. Andres Contreras¹

¹Department of Large Animal Clinical Sciences, Michigan State University, ²Department of Pharmacology and Toxicology, Michigan State University

JoVE 55818

Induction of Hypoxia in Living Frog and Zebrafish Embryos

Helena Khaliullina-Skultety¹, Ngiam Zi Chao¹, William A. Harris¹

¹Department of Physiology, Development and Neuroscience, University of Cambridge

JoVE 55710

Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos

Lorena Benedetti^1,2,3, Anna Ghilardi⁴, Laura Prosperi⁴, Maura Francolini*¹, Luca Del Giacco*⁴

¹Department of Medical Biotechnology and Translational Medicine, Università degli Studi di Milano, ²Department of Neuroscience; Department of Cell Biology, Howard Hughes Medical Institute, Yale University School of Medicine, ³Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, ⁴Department of BioSciences, Università degli Studi di Milano

JoVE 55297

Acute and Chronic Models of Hyperglycemia in Zebrafish: A Method to Assess the Impact of Hyperglycemia on Neurogenesis and the Biodistribution of Radiolabeled Molecules

Anne-Claire Dorsemans¹, Christian Lefebvre d'Hellencourt¹, Imade Ait-Arsa², Emmanuelle Jestin^1,2, Olivier Meilhac^1,3, Nicolas Diotel¹

¹UMR 1188 Diabète athérothombose Thérapie Réunion Océan Indien (DéTROI), Saint-Denis de La Réunion, France, Université de La Réunion, INSERM, ²RIPA (Radiochimie et Imagerie du Petit Animal), Cyclotron de la Réunion Océan Indien CYROI, ³CHU de La Réunion

JoVE 55203

Chondrogenic Pellet Formation from Cord Blood-derived Induced Pluripotent Stem Cells

Yoojun Nam¹, Yeri Alice Rim¹, Ji Hyeon Ju²

¹CiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Division of Rheumatology, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, ²Division of Rheumatology, Department of Internal Medicine, Seoul St. Mary's Hospital, Institute of Medical Science, College of Medicine, The Catholic University of Korea

JoVE 55988

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Vishnu V. Krishnamurthy*¹, Aurora J. Turgeon*², John S. Khamo¹, Payel Mondal¹, Savanna R. Sharum¹, Wenyan Mei², Jing Yang², Kai Zhang^1,3,4

¹Department of Biochemistry, University of Illinois at Urbana-Champaign, ²Department of Comparative Biosciences, University of Illinois at Urbana-Champaign, ³Neuroscience Program, University of Illinois at Urbana-Champaign, ⁴Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign

JoVE 55823

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Fabien Delerue¹, Lars M. Ittner¹

¹Transgenic Animal Unit, Mark Wainwright Analytical Centre, University of New South Wales

JoVE 55765

Hypoxic Preconditioning of Marrow-derived Progenitor Cells As a Source for the Generation of Mature Schwann Cells

Yat-Ping Tsui*¹, Alan Kwan-Long Mung*¹, Ying-Shing Chan¹, Daisy Kwok-Yan Shum¹, Graham Ka-Hon Shea²

¹School of Biomedical Sciences, The University of Hong Kong, ²Department of Orthopaedics and Traumatology, The University of Hong Kong

JoVE 55794

Subcutaneous Neurotrophin 4 Infusion Using Osmotic Pumps or Direct Muscular Injection Enhances Aging Rat Laryngeal Muscles

Richard D. Andreatta¹, Joseph C. Stemple¹, Tanya S. Seward², Colleen A. McMullen²

¹Department of Rehabilitation Sciences, University of Kentucky, ²Department of Physiology, University of Kentucky

JoVE 55837

Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing

Francesca Pistollato¹, David Canovas-Jorda¹, Dimitra Zagoura¹, Anna Price¹

¹Directorate F - Health, Consumers and Reference Materials, Joint Research Centre

JoVE 55702

In Ovo Electroporation in the Chicken Auditory Brainstem

Ting Lu¹, Ariel Loren Cohen¹, Jason Tait Sanchez^1,2,3

¹Roxelyn and Richard Pepper Department of Communication Sciences and Disorders, Northwestern University, ²Knowles Hearing Research Center, Northwestern University, ³Department of Neurobiology, Northwestern University

JoVE 55628

Transfer of Mammary Gland-forming Ability Between Mammary Basal Epithelial Cells and Mammary Luminal Cells via Extracellular Vesicles/Exosomes

Meng-Chieh Lin*¹, Shih-Yin Chen*¹, Pei-Lin He¹, Wen-Ting Luo¹, Hua-Jung Li¹

¹Institute of Cellular and System Medicine, National Health Research Institutes

JoVE 55736

Precise Cellular Ablation Approach for Modeling Acute Kidney Injury in Developing Zebrafish

Rohan Datta¹, Ada Wong¹, Troy Camarata², Farhana Tamanna¹, Imran Ilahi¹, Aleksandr Vasilyev¹

¹Department of Biomedical Sciences, NYITCOM, ²Department of Basic Sciences, NYITCOM-A-State

JoVE 55606

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Immunology and Infection

Biology

Genetics

Neuroscience

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