JoVE Journal

Developmental Biology

JoVE Developmental Biology includes methods for the study of biological development at all levels, from cellular to whole organisms.

‹1…6 7 8 9 10 11…17 › 404 VIDEO ARTICLES

Acute and Chronic Models of Hyperglycemia in Zebrafish: A Method to Assess the Impact of Hyperglycemia on Neurogenesis and the Biodistribution of Radiolabeled Molecules

Anne-Claire Dorsemans¹, Christian Lefebvre d'Hellencourt¹, Imade Ait-Arsa², Emmanuelle Jestin^1,2, Olivier Meilhac^1,3, Nicolas Diotel¹

¹UMR 1188 Diabète athérothombose Thérapie Réunion Océan Indien (DéTROI), Saint-Denis de La Réunion, France, Université de La Réunion, INSERM, ²RIPA (Radiochimie et Imagerie du Petit Animal), Cyclotron de la Réunion Océan Indien CYROI, ³CHU de La Réunion

JoVE 55203

Chondrogenic Pellet Formation from Cord Blood-derived Induced Pluripotent Stem Cells

Yoojun Nam¹, Yeri Alice Rim¹, Ji Hyeon Ju²

¹CiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Division of Rheumatology, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, ²Division of Rheumatology, Department of Internal Medicine, Seoul St. Mary's Hospital, Institute of Medical Science, College of Medicine, The Catholic University of Korea

JoVE 55988

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Vishnu V. Krishnamurthy*¹, Aurora J. Turgeon*², John S. Khamo¹, Payel Mondal¹, Savanna R. Sharum¹, Wenyan Mei², Jing Yang², Kai Zhang^1,3,4

¹Department of Biochemistry, University of Illinois at Urbana-Champaign, ²Department of Comparative Biosciences, University of Illinois at Urbana-Champaign, ³Neuroscience Program, University of Illinois at Urbana-Champaign, ⁴Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign

JoVE 55823

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Fabien Delerue¹, Lars M. Ittner¹

¹Transgenic Animal Unit, Mark Wainwright Analytical Centre, University of New South Wales

JoVE 55765

Hypoxic Preconditioning of Marrow-derived Progenitor Cells As a Source for the Generation of Mature Schwann Cells

Yat-Ping Tsui*¹, Alan Kwan-Long Mung*¹, Ying-Shing Chan¹, Daisy Kwok-Yan Shum¹, Graham Ka-Hon Shea²

¹School of Biomedical Sciences, The University of Hong Kong, ²Department of Orthopaedics and Traumatology, The University of Hong Kong

JoVE 55794

Subcutaneous Neurotrophin 4 Infusion Using Osmotic Pumps or Direct Muscular Injection Enhances Aging Rat Laryngeal Muscles

Richard D. Andreatta¹, Joseph C. Stemple¹, Tanya S. Seward², Colleen A. McMullen²

¹Department of Rehabilitation Sciences, University of Kentucky, ²Department of Physiology, University of Kentucky

JoVE 55837

Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing

Francesca Pistollato¹, David Canovas-Jorda¹, Dimitra Zagoura¹, Anna Price¹

¹Directorate F - Health, Consumers and Reference Materials, Joint Research Centre

JoVE 55702

In Ovo Electroporation in the Chicken Auditory Brainstem

Ting Lu¹, Ariel Loren Cohen¹, Jason Tait Sanchez^1,2,3

¹Roxelyn and Richard Pepper Department of Communication Sciences and Disorders, Northwestern University, ²Knowles Hearing Research Center, Northwestern University, ³Department of Neurobiology, Northwestern University

JoVE 55628

Transfer of Mammary Gland-forming Ability Between Mammary Basal Epithelial Cells and Mammary Luminal Cells via Extracellular Vesicles/Exosomes

Meng-Chieh Lin*¹, Shih-Yin Chen*¹, Pei-Lin He¹, Wen-Ting Luo¹, Hua-Jung Li¹

¹Institute of Cellular and System Medicine, National Health Research Institutes

JoVE 55736

Precise Cellular Ablation Approach for Modeling Acute Kidney Injury in Developing Zebrafish

Rohan Datta¹, Ada Wong¹, Troy Camarata², Farhana Tamanna¹, Imran Ilahi¹, Aleksandr Vasilyev¹

¹Department of Biomedical Sciences, NYITCOM, ²Department of Basic Sciences, NYITCOM-A-State

JoVE 55606

Medium-scale Preparation of Drosophila Embryo Extracts for Proteomic Experiments

Liu Yang¹, Sayantanee Paul¹, Sarah DuBois-Coyne¹, Phillip Kyriakakis², Alexey Veraksa¹

¹Biology Department, University of Massachusetts Boston, ²Department of Bioengineering, University of California, San Diego

JoVE 55804

Analysis of Protein-protein Interactions and Co-localization Between Components of Gap, Tight, and Adherens Junctions in Murine Mammary Glands

Elham Dianati^1,2, Isabelle Plante¹

¹INRS, Institut Armand-Frappier, ²Biomed, UQAM

JoVE 55772

Isolation of Type I and Type II Pericytes from Mouse Skeletal Muscles

Abhijit Nirwane¹, Jyoti Gautam¹, Yao Yao¹

¹College of Pharmacy, University of Minnesota

JoVE 55904

Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis

Jiyeon Ohk¹, Hosung Jung^1,2

¹Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, ²Department of Anatomy, Brain Research Institute, Yonsei University College of Medicine

JoVE 55652

Histological Analyses of Acute Alcoholic Liver Injury in Zebrafish

Jillian L. Ellis¹, Chunyue Yin^1,2

¹Division of Gastroenterology, Hepatology and Nutrition, Cincinnati Children's Hospital Medical Center, ²Division of Developmental Biology, Cincinnati Children's Hospital Medical Center

JoVE 55630

Preparation of Plasma Membrane Vesicles from Bone Marrow Mesenchymal Stem Cells for Potential Cytoplasm Replacement Therapy

Li-qun Xu¹, Mei-jia Lin¹, Yun-pan Li¹, Shuang Li¹, Shao-jun Chen¹, Chi-ju Wei¹

¹Multidisciplinary Research Center, Shantou University

JoVE 55741

Dissection and Culture of Mouse Embryonic Kidney

Bejan Aresh¹, Christiane Peuckert²

¹Department of Neuroscience, Uppsala University, ²Department of Organismal Biology, Evolutionary Biology Center, Uppsala University

JoVE 55715

A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro

Zhaofeng Jia^1,2, Yujie Liang³, Bin Ma^4,5, Xiao Xu^2,6, Jianyi Xiong², Li Duan², Daping Wang^1,2

¹Guangzhou Medical University, ²Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopeadic Engineering, Department of Orthopedics, Shenzhen Second People's Hospital (The First Hospital Affiliated to Shenzhen University), ³Department of Chemistry, The Chinese University of Hong Kong, ⁴School of Biomedical Engineering, Shanghai Jiao Tong University, ⁵Renji Hospital Clinical Stem Cell Research Center, Shanghai Jiao Tong University School of Medicine, ⁶Shantou University Medical College

JoVE 55565

A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development

Charlotte Kirchhelle¹, Ian Moore¹

¹Department of Plant Sciences, University of Oxford

JoVE 55331

Electrophysiological Analysis of human Pluripotent Stem Cell-derived Cardiomyocytes (hPSC-CMs) Using Multi-electrode Arrays (MEAs)

Luca Sala¹, Dorien Ward-van Oostwaard¹, Leon G. J. Tertoolen¹, Christine L Mummery^1,2, Milena Bellin¹

¹Department of Anatomy and Embryology, Leiden University Medical Center, ²Department of Applied Stem Cell Technologies, University of Twente

JoVE 55587

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

Charles A. C. Williams¹, Nathanael S. Gray^2,3, Greg M. Findlay¹

¹The MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, ²Department of Cancer Biology, Dana-Farber Cancer Institute, ³Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School

JoVE 55515

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Federica Franciosi¹, Irene Tessaro^1,2, Rozenn Dalbies-Tran³, Cecile Douet³, Fabrice Reigner⁴, Stefan Deleuze⁵, Pascal Papillier³, Ileana Miclea⁶, Valentina Lodde¹, Alberto M. Luciano¹, Ghylene Goudet³

¹Department of Health, Animal Science and Food Safety, University of Milan, ²IRCCS. Istituto Ortopedico Galeazzi, ³PRC, CNRS, IFCE, Université de Tours, INRA, ⁴PAO, INRA, ⁵Clinique des Animaux de Compagnie et des Équidés, Université de Liège, ⁶University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania

JoVE 55242

Long-term Live Imaging of Drosophila Eye Disc

Chia-Kang Tsao^1,2, Hui-Yu Ku^1,2, Y. Henry Sun^1,2

¹Institute of Genomic Sciences, National Yang-Ming University, ²Institute of Molecular Biology, Academia Sinica

JoVE 55748

Maturation of Human Stem Cell-derived Cardiomyocytes in Biowires Using Electrical Stimulation

Xuetao Sun¹, Sara S. Nunes^1,2,3,4

¹Toronto General Research Institute, University Health Network, ²Institute of Biomaterials and Biomedical Engineering, University of Toronto, ³Heart & Stroke/Richard Lewar Centre of Excellence, University of Toronto, ⁴Laboratory of Medicine and Pathobiology, University of Toronto

JoVE 55373

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Immunology and Infection

Biology

Genetics

Neuroscience

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