JoVE Journal

Developmental Biology

JoVE Developmental Biology includes methods for the study of biological development at all levels, from cellular to whole organisms.

‹1…6 7 8 9 10 11…25 › 588 VIDEO ARTICLES

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

Taiki Nakajima¹, Hidetoshi Sakurai², Makoto Ikeya²

¹Department of Life Science Frontiers, Center for iPS Cells Research and Application, Kyoto University, ²Department of Clinical Application, Center for iPS Cells Research and Application, Kyoto University

JoVE 59359

Generation of Organoids from Mouse Extrahepatic Bile Ducts

Junya Shiota¹, Nureen H. Mohamad Zaki¹, Juanita L. Merchant^1,2, Linda C. Samuelson^1,2, Nataliya Razumilava¹

¹Departments of Internal Medicine, The University of Michigan, ²Molecular & Integrative Physiology, The University of Michigan

JoVE 59544

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells

Yena Kim^1,2, Ji Hyeon Ju^1,2,3

¹CiSTEM Laboratory, Catholic Induced Pluripotent Stem Cell (iPSC) Research Center, College of Medicine, The Catholic University of Korea, ²Department of Biomedicine & Health Science, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, ³Division of Rheumatology, Department of Internal Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea

JoVE 59297

Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence

Zhentao Zhang^1,2,3, Young-Jae Nam^1,2,3

¹Department of Medicine, Division of Cardiovascular Medicine, Vanderbilt University Medical Center, ²Department of Cell and Developmental Biology, Vanderbilt University, ³Vanderbilt Center for Stem Cell Biology, Vanderbilt University

JoVE 59413

Thawing, Culturing, and Cryopreserving Drosophila Cell Lines

Arthur Luhur¹, Kristin M. Klueg¹, Johnny Roberts¹, Andrew C. Zelhof¹

¹Drosophila Genomics Resource Center, Department of Biology, Indiana University Bloomington

JoVE 59459

Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells

Hannah C. Leeson^1,2, Tailoi Chan-Ling^3,4, Michael D. Lovelace^3,5,6, Jeremy D. Brownlie⁷, Michael W. Weible II*^1,4,7, Ben J. Gu*⁸

¹Griffith Institute for Drug Discovery, Griffith University, ²Australian Institute for Bioengineering and Nanotechnology, University of Queensland, ³Discipline of Anatomy and Histology, School of Medical Science, University of Sydney, ⁴Bosch Institute, University of Sydney, ⁵Applied Neurosciences Program, Peter Duncan Neurosciences Research Unit, St. Vincent's Centre for Applied Medical Research, ⁶School of Medical Sciences, The University of New South Wales (UNSW) Medicine, Sydney, New South Wales, ⁷School of Environment and Science, Griffith University, Brisbane, Queensland, ⁸Florey Institute of Neuroscience and Mental Health, University of Melbourne

JoVE 59313

Confocal Live Imaging of Shoot Apical Meristems from Different Plant Species

Yuan Geng^1,2, Yun Zhou^1,2

¹Department of Botany and Plant Pathology, Purdue University, ²Purdue Center for Plant Biology, Purdue University

JoVE 59369

Electroretinogram Recording in Larval Zebrafish using A Novel Cone-Shaped Sponge-tip Electrode

Jiaheng Xie¹, Patricia R. Jusuf¹, Patrick T. Goodbourn², Bang V. Bui³

¹School of Biosciences, University of Melbourne, ²Melbourne School of Psychological Sciences, University of Melbourne, ³Department of Optometry and Vision Sciences, University of Melbourne

JoVE 59487

Visualization of the Superior Ocular Sulcus during Danio rerio Embryogenesis

Kevin H. Yoon¹, Sonya A. Widen^1,2, Melissa M. Wilson¹, Jennifer C. Hocking^2,3,4,5, Andrew J. Waskiewicz^1,2,6

¹Department of Biological Sciences, University of Alberta, ²Women & Children's Health Research Institute, University of Alberta, ³Division of Anatomy, Department of Surgery, University of Alberta, ⁴Department of Cell Biology, University of Alberta, ⁵Department of Medical Genetics, University of Alberta, ⁶Neuroscience and Mental Health Research Institute, University of Alberta

JoVE 59259

Efficient Generation of Pancreas/Duodenum Homeobox Protein 1⁺ Posterior Foregut/Pancreatic Progenitors from hPSCs in Adhesion Cultures

Taro Toyoda¹, Azuma Kimura¹, Hiromi Tanaka¹, Kenji Osafune¹

¹Center for iPS Cell Research and Application (CiRA), Kyoto University

JoVE 57641

Studying Oxidative Stress Caused by the Mitis Group Streptococci in Caenorhabditis elegans

Ali Naji¹, Ali Al Hatem¹, Ransome van der Hoeven¹

¹Department of Diagnostic and Biomedical Sciences, School of Dentistry, University of Texas Health Science Center

JoVE 59301

Combining Histochemical Staining and Image Analysis to Quantify Starch in the Ovary Primordia of Sweet Cherry during Winter Dormancy

Erica Fadon¹, Javier Rodrigo¹

¹Instituto Agroalimentario de Aragón - IA2 (CITA-Universidad de Zaragoza), Centro de Investigación y Tecnología Agroalimentaria de Aragón

JoVE 58524

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells

Stefano Martellucci^1,2, Costantino Santacroce¹, Valeria Manganelli², Francesca Santilli^1,2, Luca Piccoli³, Michele Cassetta³, Roberta Misasi², Maurizio Sorice², Vincenzo Mattei^1,2

¹Laboratory of Experimental Medicine and Environmental Pathology, Sabina Universitas - Rieti University Hub, ²Department of Experimental Medicine, Sapienza University of Rome, ³Department of Science Dentistry and Maxillofacial, Sapienza University of Rome

JoVE 59282

Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation

Miles W. DeAngelis¹, Ruth I. Johnson¹

¹Department of Biology, Wesleyan University

JoVE 59299

In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines

Edwina P. Kisanga¹, Zhonghua Tang¹, Seth Guller¹, Shannon Whirledge¹

¹Department of Obstetrics, Gynecology and Reproductive Sciences, Yale School of Medicine

JoVE 58942

Wholemount In Situ Hybridization for Astyanax Embryos

Heidi Luc¹, Connor Sears¹, Andrew Raczka¹, Joshua B. Gross¹

¹Department of Biological Sciences, University of Cincinnati

JoVE 59114

Methods Collection

Assessment of Ultrastructural Neuroplasticity Parameters After In Utero Transduction of the Developing Mouse Brain and Spinal Cord

David Lutz*¹, Monika von Düring*¹, Franco Corvace¹, Luzie Augustinowski¹, Anne-Kathrin Trampe¹, Marzena Nowak², Eckart Förster¹

¹Department of Neuroanatomy and Molecular Brain Research, Ruhr University Bochum, ²Central Animal Facility of the Medical Faculty, Ruhr University Bochum

JoVE 59084

Microinjection-based System for In Vivo Implantation of Embryonic Cardiomyocytes in the Avian Embryo

Trevor Henley¹, Kandace Thomas¹, Michael Bressan¹

¹Department of Cell Biology and Physiology, McAllister Heart Institute, University of North Carolina at Chapel Hill

JoVE 59267

Isolation of Embryonic Tissues and Formation of Quail-Chicken Chimeric Organs Using The Thymus Example

Marta Figueiredo¹, Hélia Neves¹

¹Instituto de Histologia e Biologia do Desenvolvimento, Faculdade de Medicina, Universidade de Lisboa

JoVE 58965

Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants

Sota Iwatani¹, Makiko Yoshida², Keiji Yamana¹, Daisuke Kurokawa¹, Jumpei Kuroda¹, Khin Kyae Mon Thwin¹, Suguru Uemura¹, Satoru Takafuji¹, Nanako Nino¹, Tsubasa Koda, Masami Mizobuchi⁴, Masahiro Nishiyama¹, Kazumichi Fujioka¹, Hiroaki Nagase¹, Ichiro Morioka⁵, Kazumoto Iijima¹, Noriyuki Nishimura¹

¹Department of Pediatrics, Kobe University Graduate School of Medicine, ²Department of Pathology, Kobe Children's Hospital, ³Department of Pediatrics, Hyogo College of Medicine, ⁴Department of Developmental Pediatrics, Shizuoka Children's Hospital, ⁵Department of Pediatrics, Nihon University School of Medicine

JoVE 58806

Visualizing Cellular Gibberellin Levels Using the nlsGPS1 Förster Resonance Energy Transfer (FRET) Biosensor

Annalisa Rizza¹, Ankit Walia¹, Bijun Tang¹, Alexander M. Jones¹

¹Sainsbury Laboratory, University of Cambridge

JoVE 58739

Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells

Shino Mitsunaga¹, Keiko Shioda¹, Kurt J. Isselbacher¹, Jacob H. Hanna², Toshi Shioda¹

¹Center for Cancer Research, Massachusetts General Hospital, ²Department of Molecular Genetics, Weizmann Institute of Science

JoVE 58297

Induction of Endothelial Differentiation in Cardiac Progenitor Cells Under Low Serum Conditions

Michika Mochizuki*¹, Giacomo Della Verde*¹, Habiba Soliman¹, Otmar Pfister^1,2, Gabriela M. Kuster^1,2

¹Department of Biomedicine, University Hospital and University of Basel, ²Division of Cardiology, University Hospital Basel

JoVE 58370

An In Vitro Model of a Parallel-Plate Perfusion System to Study Bacterial Adherence to Graft Tissues

Bartosz Ditkowski¹, Tiago R Veloso¹, Martyna Bezulska-Ditkowska^1,2, Andreas Lubig³, Stefan Jockenhoevel³, Petra Mela³, Ramadan Jashari⁴, Marc Gewillig¹, Bart Meyns⁵, Marc F Hoylaerts², Ruth Heying¹

¹Cardiovascular Developmental Biology, Department of Cardiovascular Sciences, KU Leuven, ²Centre for Molecular and Vascular Biology, Department of Cardiovascular Sciences, KU Leuven, ³Department of Biohybrid & Medical Textiles, AME - Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, ⁴European Homograft Bank, Saint Jean Clinique, ⁵Division of Clinical Cardiac Surgery, Department of Cardiovascular Sciences, KU Leuven

JoVE 58476

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