JoVE Journal

Developmental Biology

JoVE Developmental Biology includes methods for the study of biological development at all levels, from cellular to whole organisms.

‹1…6 7 8 9 10 11…24 › 553 VIDEO ARTICLES

Visualization of Tangential Cell Migration in the Developing Chick Optic Tectum

Yuji Watanabe¹, Chie Sakuma¹, Hiroyuki Yaginuma¹

¹Department of Neuroanatomy and Embryology, School of Medicine, Fukushima Medical University

JoVE 58506

Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells

Heidi Hongisto*¹, Meri Vattulainen*¹, Tanja Ilmarinen¹, Alexandra Mikhailova², Heli Skottman¹

¹BioMediTech Institute, Faculty of Medicine and Life Sciences, University of Tampere, ²Department of Ophthalmology, SILK, Faculty of Medicine and Life Sciences, University of Tampere

JoVE 58279

Cell Cycle Analysis in the C. elegans Germline with the Thymidine Analog EdU

Zuzana Kocsisova^1,2, Ariz Mohammad², Kerry Kornfeld¹, Tim Schedl²

¹Department of Developmental Biology, Washington University School of Medicine, St. Louis, Missouri, ²Department of Genetics, Washington University School of Medicine, St. Louis, Missouri

JoVE 58339

Micromanipulation of Chromosomes in Insect Spermatocytes

Nicolas K.H. Lin¹, Ryder Nance², Jane Szybist², Alan Cheville², Leocadia V. Paliulis³

¹Program in Cell Biology/Biochemistry, Bucknell University, ²Department of Electrical and Computer Engineering, Bucknell University, ³Biology Department, Bucknell University

JoVE 57359

Adult Zebrafish Injury Models to Study the Effects of Prednisolone in Regenerating Bone Tissue

Karina Geurtzen¹, Franziska Knopf^1,2

¹CRTD – Center for Regenerative Therapies Dresden, TU Dresden, ²Center for Healthy Aging, TU Dresden

JoVE 58429

Intravenous and Intra-amniotic In Utero Transplantation in the Murine Model

Nicholas J. Ahn¹, John D. Stratigis¹, Barbara E. Coons¹, Alan W. Flake¹, Hyun-Duck Nah-Cederquist², William H. Peranteau¹

¹Division of General, Thoracic, and Fetal Surgery, Center for Fetal Research, Children's Hospital of Philadelphia, ²Division of Plastic Reconstructive Surgery, Center for Fetal Research, Children's Hospital of Philadelphia

JoVE 58047

Use of Hematopoietic Stem Cell Transplantation to Assess the Origin of Myelodysplastic Syndrome

Yang Jo Chung¹, Ghanwa Khawaja¹, Karen M. Wolcott², Peter D. Aplan¹

¹Genetics Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, ²LGI Flow Cytometry Core Facility, Center for Cancer Research, National Cancer Institute, National Institutes of Health

JoVE 58140

A Hyperandrogenic Mouse Model to Study Polycystic Ovary Syndrome

Ping Xue¹, Zhiqiang Wang¹, Xiaomin Fu^1,2, Junjiang Wang^1,3, Gopika Punchhi¹, Andrew Wolfe^1,4, Sheng Wu^1,4,5

¹Department of Pediatrics, Johns Hopkins University School of Medicine, ²Department of Health, Beijing Military General Hospital, ³Southern Medical University, ⁴Department of Molecular and Cellular Physiology, Johns Hopkins University School of Medicine, ⁵Department of Gynecology and Obstetrics, Johns Hopkins University School of Medicine

JoVE 58379

Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells

Lin-Chen Li*^1,2, Xin-Xin Yu*^1,2, Yu-Wei Zhang*¹, Ye Feng*^1,3, Wei-Lin Qiu*^1,3, Cheng-Ran Xu¹

¹College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, ²Academy for Advanced Interdisciplinary Studies, Peking University, ³PKU-Tsinghua-NIBS Graduate Program, Peking University

JoVE 58000

An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing

Lijuan Du¹, Amy Zhou¹, Alex Sohr¹, Sougata Roy¹

¹Department of Cell Biology and Molecular Genetics, University of Maryland

JoVE 58268

Effectiveness of the Air Stripping in Two Salmonid Fish, Rainbow Trout (Oncorhynchus Mykiss) and Brown Trout (Salmo Trutta Morpha fario)

Radosław Kajetan Kowalski¹, Beata Sarosiek¹, Sylwia Judycka¹, Katarzyna Dryl¹, Joanna Grudniewska², Stefan Dobosz², Beata Irena Cejko¹

¹Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Science, ²Department of Salmonid Fish Research, The Stanisław Sakowicz Inland Fisheries Institute

JoVE 56894

A Familial Hypercholesterolemia Human Liver Chimeric Mouse Model Using Induced Pluripotent Stem Cell-derived Hepatocytes

Jiayin Yang*^1,2,6, Lai-Yung Wong*², Xiao-Yu Tian*³, Rui Wei^1,2, Wing-Hon Lai², Ka-Wing Au², Zhiwei Luo^4,5, Carl Ward^4,5, Wai-In Ho², David P. Ibañez^4,5, Hao Liu^4,5, Xichen Bao^4,5, Baoming Qin^4,5, Yu Huang³, Miguel A. Esteban^4,5,7, Hung-Fat Tse^1,2,6,7

¹Department of Medicine, University of Hong Kong-Shenzhen Hospital, ²The Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, University of Hong Kong, ³School of Biomedical Sciences, Institute of Vascular Medicine, Li Ka Shing Institute of Health Sciences, Chinese University of Hong Kong, ⁴Key Laboratory of Regenerative Biology of the Chinese Academy of Sciences, Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health and Guangzhou Medical University, ⁵Laboratory of RNA, Chromatin, and Human Disease, CAS Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, ⁶Research Centre of Heart, Brain, Hormone, and Healthy Ageing, Li Ka Shing Faculty of Medicine, University of Hong Kong, ⁷Hong Kong-Guangdong Stem Cell and Regenerative Medicine Research Centre, University of Hong Kong and Guangzhou Institutes of Biomedicine and Health

JoVE 57556

Engineering Transplantation-suitable Retinal Pigment Epithelium Tissue Derived from Human Embryonic Stem Cells

Karim Ben M'Barek^1,2,3, Walter Habeler^1,2,3, Alexandra Plancheron^1,2,3, Mohamed Jarraya⁴, Olivier Goureau⁵, Christelle Monville^1,2

¹U861, I-Stem, Association Française contre les Myopathies (AFM), Institut National de la Santé et de la Recherche Médicale (INSERM), ²U861, I-Stem, Association Française contre les Myopathies (AFM), Université Evry Val-d'Essonne (UEVE), ³I-Stem, Association Française contre les Myopathies (AFM), Centre pour L’Etude des Cellules Souches (CECS), ⁴Banque de tissus humain, Hôpital Saint Louis, Assistance Publique - Hôpitaux de Paris (AP-HP), ⁵Sorbonne Université, INSERM, CNRS, Institut de la Vision, F-75012

JoVE 58216

Defined Xeno-free and Feeder-free Culture Conditions for the Generation of Human iPSC-derived Retinal Cell Models

Amélie Slembrouck-Brec*¹, Céline Nanteau*¹, José-Alain Sahel¹, Olivier Goureau¹, Sacha Reichman¹

¹Institut de la Vision, Sorbonne Université, INSERM, CNRS, F-75012 Paris, France

JoVE 57795

Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization

Stephanie A. Michalski*¹, Sangappa B. Chadchan*¹, Emily S. Jungheim¹, Ramakrishna Kommagani¹

¹Department of Obstetrics and Gynecology, Center for Reproductive Health Sciences, Washington University School of Medicine

JoVE 57684

Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina

Ratnesh K. Singh¹, Pablo E. Diaz¹, François Binette¹, Igor O. Nasonkin¹

¹BioTime, Inc

JoVE 58274

Analysis of Epididymal Protein Synthesis and Secretion

Wei Zhou^1,2, Petra Sipilä³, Geoffry N. De Iuliis^1,2, Matthew D. Dun^2,4, Brett Nixon^1,2

¹Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, ²Hunter Medical Research Institute, ³Department of Physiology, Turku Center for Disease Modeling, Institute of Biomedicine, University of Turku, ⁴School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle

JoVE 58308

A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue

Zhenan Liu¹, Jie Wen^1,2, Xue Leng¹, Qian Zhou¹, Changkuo Zhou³, Huaqiang Zhao¹, Xunwei Wu^1,2

¹Shandong Provincial Key Laboratory of Oral Tissue Regeneration and Laboratory for Tissue Engineering and Regeneration, School of Stomatology, Shandong University, ²Suzhou Institute of Shandong University, ³Department of Urology, Qilu Hospital of Shandong University

JoVE 57784

Organotypic Culture Method to Study the Development Of Embryonic Chicken Tissues

Daniel D. T. Andrews¹, Tamara A. Franz-Odendaal¹

¹Department of Biology, Mount Saint Vincent University

JoVE 57619

A Cell-based Assay to Investigate Non-muscle Myosin II Contractility via the Folded-gastrulation Signaling Pathway in Drosophila S2R+ Cells

Kimberly A. Peters¹, Elizabeth Detmar¹, Liz Sepulveda², Corrina Del Valle², Ruth Valsquier², Anna Ritz², Stephen L. Rogers^1,3,4, Derek A. Applewhite²

¹Department of Biology, The University of North Carolina at Chapel Hill, ²Department of Biology, Reed College, ³Carolina Center for Genome Sciences, The University of North Carolina at Chapel Hill, ⁴Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill

JoVE 58325

Culture and Transfection of Zebrafish Primary Cells

Giulio Russo^1,2, Franziska Lehne¹, Sol M. Pose Méndez¹, Stefan Dübel², Reinhard W. Köster¹, Wiebke A. Sassen¹

¹Division of Cellular and Molecular Neurobiology, Zoological Institute, Braunschweig University of Technology, ²Department of Biotechnology, Institute of Biochemistry, Biotechnology and Bioinformatics, Braunschweig University of Technology

JoVE 57872

Synaptic Microcircuit Modeling with 3D Cocultures of Astrocytes and Neurons from Human Pluripotent Stem Cells

Caroline Cvetkovic¹, Nupur Basu¹, Robert Krencik¹

¹Center for Neuroregeneration, Department of Neurosurgery, Houston Methodist Research Institute

JoVE 58034

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence

Young Yeon Kim*^1,2, Jee-Hyun Um*^1,2, Jeanho Yun^1,2

¹Department of Biochemistry, College of Medicine, Dong-A University, ²Peripheral Neuropathy Research Center, College of Medicine, Dong-A University

JoVE 57890

Nanoparticle-mediated siRNA Gene-silencing in Adult Zebrafish Heart

Chenglu Xiao¹, Fang Wang², Junjie Hou¹, Xiaojun Zhu¹, Ying Luo², Jing-Wei Xiong¹

¹Beijing Key Laboratory of Cardiometabolic Molecular Medicine, State Key Laboratory of Natural and Biomimetic Drugs, Institute of Molecular Medicine, Peking University, ²Department of Biomedical Engineering, College of Engineering, Peking University

JoVE 58054

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