JoVE Journal

Developmental Biology

JoVE Developmental Biology includes methods for the study of biological development at all levels, from cellular to whole organisms.

‹1…7 8 9 10 11 12…18 › 414 VIDEO ARTICLES

Histological Analyses of Acute Alcoholic Liver Injury in Zebrafish

Jillian L. Ellis¹, Chunyue Yin^1,2

¹Division of Gastroenterology, Hepatology and Nutrition, Cincinnati Children's Hospital Medical Center, ²Division of Developmental Biology, Cincinnati Children's Hospital Medical Center

JoVE 55630

Preparation of Plasma Membrane Vesicles from Bone Marrow Mesenchymal Stem Cells for Potential Cytoplasm Replacement Therapy

Li-qun Xu¹, Mei-jia Lin¹, Yun-pan Li¹, Shuang Li¹, Shao-jun Chen¹, Chi-ju Wei¹

¹Multidisciplinary Research Center, Shantou University

JoVE 55741

Dissection and Culture of Mouse Embryonic Kidney

Bejan Aresh¹, Christiane Peuckert²

¹Department of Neuroscience, Uppsala University, ²Department of Organismal Biology, Evolutionary Biology Center, Uppsala University

JoVE 55715

A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro

Zhaofeng Jia^1,2, Yujie Liang³, Bin Ma^4,5, Xiao Xu^2,6, Jianyi Xiong², Li Duan², Daping Wang^1,2

¹Guangzhou Medical University, ²Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopeadic Engineering, Department of Orthopedics, Shenzhen Second People's Hospital (The First Hospital Affiliated to Shenzhen University), ³Department of Chemistry, The Chinese University of Hong Kong, ⁴School of Biomedical Engineering, Shanghai Jiao Tong University, ⁵Renji Hospital Clinical Stem Cell Research Center, Shanghai Jiao Tong University School of Medicine, ⁶Shantou University Medical College

JoVE 55565

A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development

Charlotte Kirchhelle¹, Ian Moore¹

¹Department of Plant Sciences, University of Oxford

JoVE 55331

Electrophysiological Analysis of human Pluripotent Stem Cell-derived Cardiomyocytes (hPSC-CMs) Using Multi-electrode Arrays (MEAs)

Luca Sala¹, Dorien Ward-van Oostwaard¹, Leon G. J. Tertoolen¹, Christine L Mummery^1,2, Milena Bellin¹

¹Department of Anatomy and Embryology, Leiden University Medical Center, ²Department of Applied Stem Cell Technologies, University of Twente

JoVE 55587

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

Charles A. C. Williams¹, Nathanael S. Gray^2,3, Greg M. Findlay¹

¹The MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, ²Department of Cancer Biology, Dana-Farber Cancer Institute, ³Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School

JoVE 55515

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Federica Franciosi¹, Irene Tessaro^1,2, Rozenn Dalbies-Tran³, Cecile Douet³, Fabrice Reigner⁴, Stefan Deleuze⁵, Pascal Papillier³, Ileana Miclea⁶, Valentina Lodde¹, Alberto M. Luciano¹, Ghylene Goudet³

¹Department of Health, Animal Science and Food Safety, University of Milan, ²IRCCS. Istituto Ortopedico Galeazzi, ³PRC, CNRS, IFCE, Université de Tours, INRA, ⁴PAO, INRA, ⁵Clinique des Animaux de Compagnie et des Équidés, Université de Liège, ⁶University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania

JoVE 55242

Long-term Live Imaging of Drosophila Eye Disc

Chia-Kang Tsao^1,2, Hui-Yu Ku^1,2, Y. Henry Sun^1,2

¹Institute of Genomic Sciences, National Yang-Ming University, ²Institute of Molecular Biology, Academia Sinica

JoVE 55748

Maturation of Human Stem Cell-derived Cardiomyocytes in Biowires Using Electrical Stimulation

Xuetao Sun¹, Sara S. Nunes^1,2,3,4

¹Toronto General Research Institute, University Health Network, ²Institute of Biomaterials and Biomedical Engineering, University of Toronto, ³Heart & Stroke/Richard Lewar Centre of Excellence, University of Toronto, ⁴Laboratory of Medicine and Pathobiology, University of Toronto

JoVE 55373

Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos

Frederic Strobl^1,2,3, Selina Klees^1,2,3, Ernst H. K. Stelzer^1,2,3

¹Physical Biology, Buchmann Institute for Molecular Life Sciences (BMLS), ²Cluster of Excellence Frankfurt, Macromolecular Complexes, ³Goethe-Universität Frankfurt am Main – Campus Riedberg

JoVE 55629

Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange

Tim Pieters^1,2,3,4, Lieven Haenebalcke^1,2, Kenneth Bruneel^1,2,4, Niels Vandamme^1,2,4, Tino Hochepied^1,2, Jolanda van Hengel⁵, Dagmar Wirth⁶, Geert Berx^1,2,4, Jody J. Haigh⁷, Frans van Roy^1,2,4, Steven Goossens^1,2,3,4

¹Department of Biomedical Molecular Biology, Ghent University, ²Inflammation Research Center, VIB, ³Center for Medical Genetics, Ghent University Hospital, ⁴Cancer Research Institute Ghent (CRIG), ⁵Department of Basic Medical Sciences, Faculty of Medicine and Health Sciences, Ghent University, ⁶Helmholtz Center for Infection Research, ⁷Mammalian Functional Genetics Laboratory, Division of Blood Cancers, Australian Centre for Blood Diseases, Department of Clinical Haematology, Monash University and Alfred Health Alfred Centre

JoVE 55575

Dissection of Larval Zebrafish Gonadal Tissue

Xinjian Wang*¹, Sijie Chen*¹, Wei Zhang¹, Yiyuan Ren¹, Quan Zhang¹, Gang Peng¹

¹Institutes of Brain Science, State Key Laboratory of Medical Neurobiology and Collaborative Innovation Center for Brain Science, Fudan University

JoVE 55294

Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies

Junning Yang¹, Chuanshen Wu², Ioana Stefanescu¹, Arie Horowitz^1,3

¹Department of Medicine, Cardeza Vascular Research Center, Sidney Kimmel Medical College, Thomas Jefferson University, ²Department of Molecular Cardiology, Cleveland Clinic Foundation, ³Department of Cancer Biology, Cardeza Vascular Research Center, Sidney Kimmel Medical College, Thomas Jefferson University

JoVE 55621

Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish

Elaine L. Welch*¹, Celeste C. Eno*¹, Sreelaja Nair², Robin E. Lindeman³, Francisco Pelegri¹

¹Laboratory of Genetics, University of Wisconsin-Madison, ²Department of Biological Sciences, Tata Institute of Fundamental Research, ³Department of Genetics, Cell Biology, and Development, University of Minnesota

JoVE 55213

Surgical Ablation Assay for Studying Eye Regeneration in Planarians

Jacob M. Morton*¹, Marwa A. Saad*¹, Wendy S. Beane¹

¹Department of Biological Sciences, Western Michigan University

JoVE 55594

Protocols for Visualizing Steroidogenic Organs and Their Interactive Organs with Immunostaining in the Fruit Fly Drosophila melanogaster

Eisuke Imura*¹, Yuto Yoshinari*¹, Yuko Shimada-Niwa*², Ryusuke Niwa³

¹Graduate School of Life and Environmental Sciences, University of Tsukuba, ²Life Science Center of Tsukuba Advanced Research Alliance, University of Tsukuba, ³Faculty of Life and Environmental Sciences, University of Tsukuba

JoVE 55519

Generation of iPSC-derived Human Brain Organoids to Model Early Neurodevelopmental Disorders

Elke Gabriel¹, Jay Gopalakrishnan^1,2

¹Center for Molecular Medicine Cologne, University of Cologne, ²Institute for Biochemistry I, Medical School of University of Cologne

JoVE 55372

Using Primary Neurosphere Cultures to Study Primary Cilia

Issei S. Shimada¹, Hemant Badgandi¹, Bandarigoda N. Somatilaka¹, Saikat Mukhopadhyay¹

¹Department of Cell Biology, University of Texas Southwestern Medical Center

JoVE 55315

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Kathryn E. Ware¹, Shivee Gilja¹, Shenghan Xu¹, Samantha Shetler¹, Mohit K. Jolly², Xueyang Wang³, Suzanne Bartholf Dewitt⁴, Alexander J. Hish¹, Sarah Jordan¹, William Eward⁵, Herbert Levine², Andrew J. Armstrong⁴, Jason A. Somarelli¹

¹Department of Medicine, Duke University, ²Department of Bioengineering, Rice University, ³Department of Molecular Genetics and Microbiology, Duke University, ⁴Solid Tumor Program and the Duke Prostate Center, Duke University Medical Center, ⁵Duke University Medical Center

JoVE 55520

Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta

Naimisha Beeravolu^1,2, Christina McKee^1,2, Ali Alamri^1,2, Sasha Mikhael³, Christina Brown^1,2, Mick Perez-Cruet^2,4, G. Rasul Chaudhry^1,2

¹Department of Biological Sciences, Oakland University, ²OU-WB Institute for Stem Cell and Regenerative Medicine, ³Department of Obstetrics and Gynecology, St. John Provindence - Providence Park Hospital, ⁴Department of Neurosurgery, Beaumont Health System

JoVE 55224

Live Confocal Imaging of Developing Arabidopsis Flowers

Nathanaël Prunet¹

¹Department of Biology and Biological Engineering, California Institute of Technology

JoVE 55156

In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences

Stefanie Dudczig^1,2, Peter D. Currie², Lucia Poggi³, Patricia R. Jusuf^1,2

¹School of Biosciences, The University of Melbourne, ²Australian Regenerative Medicine Institute (ARMI), Monash University, ³The David J Apple Center for Vision Research, Department of Ophthalmology, Heidelberg University

JoVE 55490

Assessing Cardiomyocyte Subtypes Following Transcription Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts

Antonio Fernandez-Perez¹, Nikhil V. Munshi¹

¹Department of Internal Medicine- Cardiology, UT Southwestern Medical Center

JoVE 55456

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Immunology and Infection

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