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JoVE Journal

Genetics

JoVE Genetics includes experimental approaches to study gene function, gene expression and regulation, epigenetics, genetic disorders, population genetics, and evolution.

‹1 2 3 4 5 6 7 8 9…14 › 332 VIDEO ARTICLES

Methylated RNA Immunoprecipitation Assay to Study m⁵C Modification in Arabidopsis

Eleftheria Saplaoura*¹, Valentina Perrera*², Friedrich Kragler¹

¹Max Planck Institute of Molecular Plant Physiology, ²Department of Molecular Medicine, Medical School, University of Padua

Direct Liquid-Culture Screening for Evaluating the Production of Heterologous Proteins Using an Auxotrophic Mutant of Aspergillus Oryzae

Satoru Shinkawa¹, Shigenobu Mitsuzawa¹

¹Honda Research Institute Japan Co., Ltd.

Introducing Point Mutations into Human Pluripotent Stem Cells using Seamless Genome Editing

Yu Wang¹, Andrew J. H. Smith¹, David C. Hay¹

¹MRC Centre for Regenerative Medicine, University of Edinburgh

Methods Collection

Exon Skipping in Directly Reprogrammed Myotubes Obtained from Human Urine-Derived Cells

Hotake Takizawa¹, Mitsuto Sato¹, Yoshitsugu Aoki¹

¹Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry

Identification of Enhancer-Promoter Contacts in Embryoid Bodies by Quantitative Chromosome Conformation Capture (4C)

Tian V. Tian^1,2,3, Enrique Vidal^1,2, Thomas Graf^1,2, Grégoire Stik^1,2

¹Center for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, ²Universitat Pompeu Fabra, ³Vall d'Hebron Institute of Oncology

Engineering Intracellular Protein Sensors in Mammalian Cells

Marina Duhkinova¹, Claudia Crina¹, Ron Weiss², Velia Siciliano¹

¹Istituto Italiano di Tecnologia, ²Synthetic Biology Center, Department of Biological Engineering, Massachusetts Institute of Technology

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Hirofumi Nishizono*^1,2,3, Mohamed Darwish*^4,5, Hideki Uosaki^6,7, Nanami Masuyama^8,9,10, Motoaki Seki^8,11, Hiroyuki Abe³, Nozomu Yachie^8,9,10,12,13, Ryohei Yasuda¹

¹Max Planck Florida Institute for Neuroscience, ²Life Science Research Center, University of Toyama, ³Department of Biochemical Engineering, Graduate School of Science and Engineering, Yamagata University, ⁴Graduate School of Innovative Life Science, University of Toyama, ⁵Department of Biochemistry, Faculty of Pharmacy, Cairo University, ⁶Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, ⁷Division of Stem Cell Research and Drug Development, Center for Development of Advanced Medical Technology, Jichi Medical University, ⁸Synthetic Biology Division, Research Center for Advanced Science and Technology, University of Tokyo, ⁹Institute for Advanced Biosciences, Keio University, ¹⁰Graduate School of Media and Governance, Keio University, ¹¹Department of Molecular Oncology, Graduate School of Medicine, Chiba University, ¹²Department of Biological Sciences, School of Science, University of Tokyo, ¹³College of Arts and Sciences, University of Tokyo

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Krzysztof J. Skowronek¹, Matthias Bochtler¹

¹International Institute of Molecular and Cell Biology, Warsaw (IIMCB)

Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage

Cheryl A. Hobbs*¹, Leslie Recio¹, John Winters¹, Kristine L. Witt²

¹Toxicology Program, ILS, Inc., ²Division of the National Toxicology Program, National Institute of Environmental Health Sciences

A Screening Method for Identification of Heterochromatin-Promoting Drugs Using Drosophila

Lin Zhang¹, Kenny Dao¹, Angela Kang¹, Andre C. Loyola¹, Robin Shang¹, Jinghong Li¹, Willis X. Li¹

¹Department of Medicine, University of California San Diego

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Bas B. Oude Munnink¹, David F. Nieuwenhuijse¹, Reina S. Sikkema¹, Marion Koopmans¹

¹ErasmusMC, Department of Viroscience, WHO Collaborating Centre for Arbovirus and Viral Hemorrhagic Fever Reference and Research, Erasmus University Medical Center

Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat (Fagopyrum tataricum)

Yaolei Mi^1,2, Zhihui Zhu³, Guangtao Qian³, Yu Li⁴, Xiangxiao Meng¹, Jianping Xue³, Qingfu Chen⁵, Wei Sun¹, Yuhua Shi^1,2

¹Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, ²Artemisinin Research Center, China Academy of Chinese Medical Sciences, ³College of Life Science, Huaibei Normal University, ⁴Economic Crop Research Institute Sichuan Academy of Agriculture Sciences, ⁵Research Center of Buckwheat Industry Technology, Guizhou Normal University

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Rafael J. L. Morcillo¹, Achen Zhao^1,2, María I. Tamayo-Navarrete³, José M. García-Garrido³, Alberto P. Macho¹

¹Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, ²University of Chinese Academy of Sciences, ³Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín (CSIC)

Combining Laser Capture Microdissection and Microfluidic qPCR to Analyze Transcriptional Profiles of Single Cells: A Systems Biology Approach to Opioid Dependence

Sean J. O'Sullivan^1,2, Beverly A.S. Reyes³, Rajanikanth Vadigepalli¹, Elisabeth J. Van Bockstaele³, James S. Schwaber¹

¹Daniel Baugh Institute for Functional Genomics and Computational Biology, Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, ²Sidney Kimmel Medical College, Thomas Jefferson University, ³Department of Pharmacology & Physiology, Drexel University College of Medicine

Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells

Francis Migneault^1,2,3, Frédéric Gagnon^2,3, Emmanuelle Brochiero^1,3, Yves Berthiaume^2,3, André Dagenais^2,3

¹Centre de Recherche du Centre hospitalier de l'Université de Montréal (CRCHUM), ²Institut de Recherches Cliniques de Montréal (IRCM), ³Département de médecine, Faculté de médecine, Université de Montréal

Preparation of Meiotic Chromosome Spreads from Zebrafish Spermatocytes

Yana P. Blokhina^1,2, Ivan Olaya^1,2, Sean M. Burgess¹

¹Department of Molecular and Cellular Biology, University of California, Davis, ²Integrative Genetics and Genomics Graduate Group, University of California, Davis

Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids

Anne-Marlen Gaebler¹, Alexander Hennig¹, Katharina Buczolich¹, Jürgen Weitz¹, Thilo Welsch¹, Daniel E. Stange¹, Kristin Pape¹

¹Department of Visceral-, Thoracic and Vascular Surgery, University Hospital Carl Gustav Carus, Technische Universität Dresden

Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA

Nandaraj Taye¹, Sarah Stanley¹, Dirk Hubmacher¹

¹Orthopaedic Research Laboratories, Leni & Peter W. May Department of Orthopaedics, Icahn School of Medicine at Mount Sinai

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

Jinxia Shi¹, Yijuan Jia¹, Di Fang¹, Shidan He¹, Peng Zhang^1,2, Yushuang Guo³, Yongli Qiao¹

¹Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, ²College of Agriculture, Yangtze University, ³Laboratory of Molecular Genetics, China National Tobacco Corporation, Guizhou Institute of Tobacco Science

LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles

Snehadri Sinha*^1,2, Bettina Mannerström*^1,2, Riitta Seppänen-Kaijansinkko^1,2, Sippy Kaur^1,2

¹Department of Oral and Maxillofacial Diseases, University of Helsinki, ²Helsinki University Hospital

Generation of Chimeric Axolotls with Mutant Haploid Limbs Through Embryonic Grafting

Lucas D. Sanor¹, G. Parker Flowers¹, Craig M. Crews¹

¹Department of Molecular, Cellular, and Developmental Biology, Yale University

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells

Federica Marasca¹, Alice Cortesi¹, Lara Manganaro¹, Beatrice Bodega¹

¹Istituto Nazionale di Genetica Molecolare "Romeo ed Enrica Invernizzi", INGM

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

Ying Wang*^1,2, Zheng Kuang*^1,2, Lei Li², Xiaozeng Yang¹

¹Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, ²State Key Laboratory of Protein and Plant Gene Research, Peking-Tsinghua Center for Life Sciences, School of Advanced Agricultural Sciences and School of Life Sciences, Peking University

Discovering CsgD Regulatory Targets in Salmonella Biofilm Using Chromatin Immunoprecipitation and High-Throughput Sequencing (ChIP-seq)

Melissa B. Palmer^1,2, Yejun Wang³, Aaron P. White^1,2

¹Vaccine and Infectious Disease Organization-International Vaccine Centre, ²Department of Biochemistry, Microbiology and Immunology, University of Saskatchewan, ³Department of Cell Biology and Genetics, Shenzhen University Health Science Center

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