JoVE Journal

Genetics

JoVE Genetics includes experimental approaches to study gene function, gene expression and regulation, epigenetics, genetic disorders, population genetics, and evolution.

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The TreadWheel: Interval Training Protocol for Gently Induced Exercise in Drosophila melanogaster

Kelsey E. Lowman¹, Brélahn J. Wyatt¹, Owen P. Cunneely¹, Laura K. Reed¹

¹Department of Biological Sciences, University of Alabama

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

Julia B. Carleton¹, Kristofer C. Berrett¹, Jason Gertz¹

¹Department of Oncological Sciences, Huntsman Cancer Institute, University of Utah

Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization

Caitlin E. Moss¹, Andrew Robson², Erol Fikrig³, Sukanya Narasimhan³

¹Department of Microbial Pathogenesis, Yale University School of Medicine, ²Program in Vertebrate Developmental Biology, Departments of Pediatrics and Genetics, Yale University School of Medicine, ³Department of Internal Medicine, Yale University School of Medicine

Adaptation of Hybridization Capture of Chromatin-associated Proteins for Proteomics to Mammalian Cells

Hector Guillen-Ahlers^1,2, Prahlad K. Rao¹, Danu S. Perumalla¹, Maria J. Montoya¹, Avinash Y.L. Jadhav¹, Michael R. Shortreed³, Lloyd M. Smith³, Michael Olivier^1,2

¹Department of Genetics, Texas Biomedical Research Institute, ²Department of Internal Medicine-Molecular Medicine, Wake Forest University School of Medicine, ³Department of Chemistry, University of Wisconsin

Measuring Exercise Levels in Drosophila melanogaster Using the Rotating Exercise Quantification System (REQS)

Louis P. Watanabe¹, Nicole C. Riddle¹

¹Department of Biology, University of Alabama at Birmingham

Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis

Jingxun Chen¹, David McSwiggen², Elçin Ünal¹

¹Department of Molecular and Cell Biology, Barker Hall, University of California, Berkeley, ²Department of Molecular and Cell Biology, Li Ka Shing Center, University of California, Berkeley

Canalostomy As a Surgical Approach to Local Drug Delivery into the Inner Ears of Adult and Neonatal Mice

Jing-Ying Guo¹, Lu He¹, Teng-Fei Qu¹, Yu-Ying Liu², Ke Liu¹, Guo-Peng Wang¹, Shu-Sheng Gong¹

¹Department of Otolaryngology-Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, ²Department of Otolaryngology-Head and Neck Surgery, Shanghai First People's Hospital, Shanghai Jiao Tong University

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Behnom Farboud*^1,2, Erin Jarvis*¹, Theodore L. Roth*^3,4,5,6, Jiyung Shin*^1,3, Jacob E. Corn^1,3, Alexander Marson^3,5,6,7,8,9, Barbara J. Meyer^1,2, Nipam H. Patel^1,10, Megan L. Hochstrasser³

¹Department of Molecular Cell Biology, University of California, Berkeley, ²Howard Hughes Medical Institute, University of California, Berkeley, ³Innovative Genomics Institute, University of California, Berkeley, ⁴Biomedical Sciences Graduate Program, University of California, San Francisco, ⁵Department of Microbiology and Immunology, University of California, San Francisco, ⁶Diabetes Center, University of California, San Francisco, ⁷Chan Zuckerberg Biohub, ⁸Department of Medicine, University of California, San Francisco, ⁹UCSF Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, ¹⁰Department of Integrative Biology, University of California, Berkeley

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

Christoph N. Schlaffner^1,2,3, Georg J. Pirklbauer², Andreas Bender³, Judith A.J. Steen¹, Jyoti S. Choudhary^2,4

¹Department of Neurobiology, F. M. Kirby Neurobiology Center, Boston Children's Hospital, Harvard Medical School, ²Proteomic Mass Spectrometry, Wellcome Trust Sanger Institute, Wellcome Genome Campus, ³Centre for Molecular Informatics, Department of Chemistry, University of Cambridge, ⁴Functional Proteomics Group, Chester Beatty Laboratories, Institute of Cancer Research

Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury

Zhihong Yuan^1,2, Brahmchetna Bedi¹, Ruxana T. Sadikot^1,2

¹Department of Veterans Affairs, Atlanta VAMC, ²Division of Pulmonary and Critical Care Medicine, Department of Medicine, Emory University

High-throughput Identification of Synergistic Drug Combinations by the Overlap² Method

Morgan A. Wambaugh¹, Jessica C. S. Brown¹

¹Microbiology and Immunology Division, Department of Pathology, University of Utah

Methodology for Accurate Detection of Mitochondrial DNA Methylation

Mie Mechta¹, Lars Roed Ingerslev¹, Romain Barrès¹

¹The Novo Nordisk Foundation for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast

Hogyu David Seo¹, Daeyoup Lee¹

¹Department of Biological Sciences, Korea Advanced Institute of Science and Technology

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

Seoghyun Lee*¹, Minho Won*², Ran Hee Hwang³, Gang Min Hur², Hyunju Ro¹

¹Department of Biological Sciences, College of Bioscience and Biotechnology, Chungnam National University, ²Department of Pharmacology, College of Medicine, Chungnam National University, ³Department of Nursing, Gwangju Women's University

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

Olivier Loudig^1,2,3, Christina Liu^1,2, Thomas Rohan³, Iddo Z. Ben-Dov⁴

¹Department of Research, Hackensack University Medical Center, ²Department of Medical Sciences, Seton Hall University, ³Department of Epidemiology and Population Health, Albert Einstein College of Medicine, ⁴Department of Nephrology and Hypertension, Hadassah - Hebrew University Medical Center

Investigation of RNA Synthesis Using 5-Bromouridine Labelling and Immunoprecipitation

Rikke H. Kofoed¹, Cristine Betzer¹, Søren Lykke-Andersen², Ewa Molska², Poul H. Jensen¹

¹DANDRITE - Danish Research Institute of Translational Neuroscience, Dept. of Biomedicine, Aarhus University, ²Dept. of Molecular Biology and Genetics, Aarhus University

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Tuesday Simmons¹, Daniel F. Caddell¹, Siwen Deng¹, Devin Coleman-Derr^1,2

¹Department of Plant and Microbial Biology, University of California, Berkeley, ²Plant Gene Expression Center, USDA ARS

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

Jose Antonio Escudero, R Craig MacLean, Alvaro San Millan

¹Department of Animal Health, Universidad Complutense de Madrid, ²Visavet Health Surveillance Centre, Universidad Complutense de Madrid, ³Department of Zoology, University of Oxford, ⁴Department of Microbiology, Hospital Universitario Ramon y Cajal (IRYCIS) and CIBERESP

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Harriet Prior*¹, Lauren MacConnachie*¹, Jose L. Martinez¹, Georgina C.B. Nicholl¹, Asim A. Beg¹

¹Department of Pharmacology, University of Michigan

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

Joseph C. Siefert^1,2, Emily A. Clowdus^1,2, Duane Goins¹, Amnon Koren³, Christopher L. Sansam^1,2

¹Cell Cycle and Cancer Biology Research Program, Oklahoma Medical Research Foundation, ²Department of Cell Biology, University of Oklahoma Health Sciences Center, ³Department of Molecular Biology and Genetics, Cornell University

A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel

Ling Huang*¹, Xiaohui Deng*¹, Ronghua Li¹, Yanshi Xia¹, Guihua Bai², Kadambot H.M. Siddique³, Peiguo Guo¹

¹College of Life Sciences, Guangzhou University, ²Hard Winter Wheat Genetics Research Unit, United States Department of Agriculture - Agricultural Research Service, ³The UWA Institute of Agriculture, University of Western Australia

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

Diego Marin*^1,2, Alexandra L. Nguyen*³, Richard T. Scott, Jr.^1,2, Karen Schindler³

¹IVI-RMA New Jersey, ²Jefferson College of Biomedical Sciences, Thomas Jefferson University, ³Department of Genetics, Rutgers, The State University of New Jersey

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Manon Torres¹, Denis Becquet¹, Séverine Guillen¹, Bénédicte Boyer¹, Mathias Moreno¹, Marie-Pierre Blanchard², Jean-Louis Franc¹, Anne-Marie François-Bellan¹

¹CNRS, CRN2M-UMR7286, Faculté de Médecine Nord, Aix-Marseille Université, ²Plate-forme d'imagerie MRI, UMS3426, CNRS 141, Institut de Génétique Humaine

Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9

Lorenzo Brunetti*^1,2,3, Michael C. Gundry*^1,2,4, Ayumi Kitano⁴, Daisuke Nakada^1,2,4, Margaret A. Goodell^1,2,4,5

¹Stem Cells & Regenerative Medicine Center, Baylor College of Medicine, ²Center for Cell and Gene Therapy, Baylor College of Medicine, ³Centro di Ricerca Emato-Oncologica (CREO), University of Perugia, ⁴Department of Molecular & Human Genetics, Baylor College of Medicine, ⁵Texas Children's Hospital & Houston Methodist Hospital

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