JoVE Journal

Genetics

JoVE Genetics includes experimental approaches to study gene function, gene expression and regulation, epigenetics, genetic disorders, population genetics, and evolution.

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Chromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines

Benedetto Daniele Giaimo¹, Francesca Ferrante¹, Tilman Borggrefe¹

¹Institute of Biochemistry, University of Giessen

JoVE 55907

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Erin L. Sternburg¹, Kristen C. Dias¹, Fedor V. Karginov¹

¹Cell Biology and Neuroscience, University of California, Riverside

JoVE 55903

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Santhilal Subhash¹, Meena Kanduri²

¹Department of Medical Genetics, Institute of Biomedicine, Gothenburg University, ²Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine, Gothenburg University

JoVE 55773

Protocols for C-Brick DNA Standard Assembly Using Cpf1

Shi-Yuan Li¹, Guo-Ping Zhao¹, Jin Wang¹

¹Institute of Plant Physiology and Ecology, Shanghai Insititutes for Biological Sciences, Chinese Academy of Sciences

JoVE 55775

Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow

Gajendra W. Suryawanshi*^1,2, Song Xu*³, Yiming Xie¹, Tom Chou³, Namshin Kim⁴, Irvin S. Y. Chen^1,5, Sanggu Kim⁶

¹UCLA AIDS Institute, University of California at Los Angeles (UCLA), ²Department of Microbiology, Immunology, & Molecular Genetics, University of California at Los Angeles (UCLA), ³Departments of Biomathematics and Mathematics, University of California at Los Angeles (UCLA), ⁴Personalized Genomic Medicine Research Center, Division of Strategic Research Groups, Korea Research Institute of Bioscience and Biotechnology, ⁵Department of Medicine, University of California at Los Angeles (UCLA), ⁶Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University (OSU)

JoVE 55812

Rapid Deletion Production in Fungi via Agrobacterium Mediated Transformation of OSCAR Deletion Constructs

Scott E. Gold¹, Zahi Paz², María D. García-Pedrajas³, Anthony E. Glenn¹

¹Toxicology and Mycotoxin Research Unit, NPRC, USDA-ARS, ²Hazera Seeds LTD, Brurim, ³Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora” - Universidad de Málaga - Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Estación Experimental “La Mayora”

JoVE 55239

Flow-sorting and Exome Sequencing of the Reed-Sternberg Cells of Classical Hodgkin Lymphoma

Jonathan B. Reichel¹, Jason McCormick², Jonathan R. Fromm³, Olivier Elemento⁴, Ethel Cesarman⁵, Mikhail Roshal⁶

¹Innovation Laboratory, Center for Molecular Oncology, Memorial Sloan Kettering Cancer Center, ²Flow-Sorting Core Facility, Weill Cornell Medical College, ³Department of Laboratory Medicine, University of Washington, ⁴Department of Physiology and Biophysics, Institute for Computational Biomedicine, Weill Cornell Medical College, ⁵Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, ⁶Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center

JoVE 54399

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Aintzane Apraiz*¹, Jone Mitxelena*^2,3, Ana Zubiaga²

¹Department of Cell Biology and Histology, University of the Basque Country, UPV/EHU, ²Department of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country, UPV/EHU, ³Department of Molecular Mechanisms of Disease, University of Zurich

JoVE 55745

Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae

Sebastian Grünberg¹, Gabriel E. Zentner²

¹Basic Sciences Division, Fred Hutchinson Cancer Research Center, ²Department of Biology, Indiana University

JoVE 55836

Quantifying Abdominal Pigmentation in Drosophila melanogaster

Omid Saleh Ziabari¹, Alexander W. Shingleton¹

¹Department of Biology, Lake Forest College

JoVE 55732

Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions

Oriana Romano^1,2, Ingrid Cifola³, Valentina Poletti⁴, Marco Severgnini³, Clelia Peano³, Gianluca De Bellis³, Fulvio Mavilio^1,4, Annarita Miccio^1,2,4,5

¹Center for Genome Research, Department of Life Sciences, University of Modena and Reggio Emilia, ²Laboratory of Chromatin and Gene Regulation During Development, Imagine Institute, ³Institute for Biomedical Technologies, CNR, ⁴Généthon, ⁵Sorbonne Paris Cité - Université Paris Descartes

JoVE 55919

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Jonas Gross*¹, Nicolas Wirth*¹, Ingrid Tessmer¹

¹Rudolf Virchow Center for Experimental Biomedicine, University of Würzburg

JoVE 55501

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Jong Ghut Ashley Aw¹, Yang Shen², Niranjan Nagarajan², Yue Wan¹

¹Stem Cell and Regenerative Biology, Genome Institute of Singapore, A*STAR, ²Computational and Systems Biology, Genome Institute of Singapore, A*STAR

JoVE 55255

Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved (Non-model) Organisms

Blake L. Joyce^1,2, Asher K. Haug-Baltzell³, Jonathan P. Hulvey⁴, Fiona McCarthy⁵, Upendra Kumar Devisetty^1,6, Eric Lyons^1,2,3

¹BIO5 Institute, University of Arizona, ²The School of Plant Sciences, University of Arizona, ³Genetics GIDP, University of Arizona, ⁴Biology Department, University of Massachusetts Amherst, ⁵School of Animal and Comparative Biomedical Sciences, University of Arizona, ⁶CyVerse, University of Arizona