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JoVE Journal

Genetics

JoVE Genetics includes experimental approaches to study gene function, gene expression and regulation, epigenetics, genetic disorders, population genetics, and evolution.

‹1…3 4 5 6 7 8 9…13 › 312 VIDEO ARTICLES

CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation

Linyuan Ma*¹, Lydia Jang*¹, Jian Chen², Jun Song¹, Dongshan Yang¹, Jifeng Zhang¹, Y. Eugene Chen¹, Jie Xu¹

¹Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical Center, ²Celetrix LLC

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis

Snorre Gulla¹, Saima Nasrin Mohammad¹, Duncan John Colquhoun^1,2

¹Fish Health Research Group, Norwegian Veterinary Institute, ²University of Bergen

Chromogenic In Situ Hybridization as a Tool for HPV-Related Head and Neck Cancer Diagnosis

Sophie Outh-Gauer¹, Jérémy Augustin¹, Marion Mandavit², Ophélie Grard², Thomas Denize¹, Marine Nervo¹, Charles Lépine¹, Marc Rassy², Eric Tartour^2,3, Cécile Badoual^1,2

¹Department of Pathology, Georges Pompidou European Hospital, Assistance publique - Hôpitaux de Paris (APHP), Paris Descartes University, ²Paris Cardiovascular Research Center (PARCC), Institut national de la santé et de la recherche médicale (Inserm) U970, ³Laboratory of Immunology, Georges Pompidou European Hospital, APHP, Paris Descartes University

Generation of Tumor Organoids from Genetically Engineered Mouse Models of Prostate Cancer

Kristine M. Wadosky¹, Yanqing Wang¹, Xiaojing Zhang¹, David W. Goodrich¹

¹Department of Pharmacology & Therapeutics, Roswell Park Comprehensive Cancer Center

The Use of Mouse Mammary Tumor Cells in an In Vitro Invasion Assay as a Measure of Oncogenic Cell Behavior

John A. Schmidt¹, Janice E. Knepper¹

¹Department of Biology, Villanova University

An Assay for Quantifying Protein-RNA Binding in Bacteria

Noa Katz*¹, Roni Cohen*¹, Orna Atar¹, Sarah Goldberg¹, Roee Amit^1,2

¹Department of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, ²Russell Berrie Nanotechnology Institute, Technion-Israel Institute of Technology

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Andreas Mueller¹, Kerstin Fischer², Roland Suluku³, Thomas Hoenen¹

¹Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, ²Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, ³Department of Animal Science, Njala University

A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome

Piotr Przanowski¹, Zeming Zheng¹, Urszula Wasko¹, Sanchita Bhatnagar^1,2

¹Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, ²Department of Neuroscience, University of Virginia School of Medicine

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Hyun Ah Seo¹, Cho Yean Hwang¹, Sokviseth Moeng¹, Jong Kook Park¹

¹Department of Biomedical Science and Research Institute for Bioscience & Biotechnology, Hallym University

RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level

Tiffany Kaul¹, Maria E. Morales¹, Emily Smither¹, Melody Baddoo^1,2, Victoria P. Belancio^1,3, Prescott Deininger^1,4

¹Tulane Cancer Center, Tulane University, ²Department of Pathology, Tulane University, ³Department of Structural and Cellular Biology, Tulane University, ⁴Department of Epidemiology, Tulane University

Designing Automated, High-throughput, Continuous Cell Growth Experiments Using eVOLVER

Zachary J. Heins^1,2, Christopher P. Mancuso^1,2, Szilvia Kiriakov^1,3, Brandon G. Wong^1,2, Caleb J. Bashor⁴, Ahmad S. Khalil^1,2,5

¹Biological Design Center, Boston University, ²Department of Biomedical Engineering, Boston University, ³Program in Molecular Biology, Cell Biology and Biochemistry, Boston University, ⁴Department of Bioengineering, Rice University, ⁵Wyss Institute for Biologically Inspired Engineering, Harvard University

Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates

Siming Liu^1,2, Mikako Harata^1,2, Joseph A. Promes^1,2, Anthony J. Burand^2,3, James A. Ankrum^2,3, Yumi Imai^1,2

¹Department of Internal Medicine, Carver College of Medicine, University of Iowa, ²Fraternal Order of Eagles Diabetes Research Center, University of Iowa, ³Roy J. Carver Department of Biomedical Engineering, University of Iowa

Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella

Jeff A. Shaw¹, Travis J. Bourret¹

¹Department of Medical Microbiology and Immunology, Creighton University School of Medicine

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Melvin Noe Gonzalez¹, Joan W. Conaway^1,2, Ronald C. Conaway^1,2

¹Stowers Institute for Medical Research, ²Department of Biochemistry and Molecular Biology, Kansas University Medical Center

Intraventricular Transplantation of Engineered Neuronal Precursors for In Vivo Neuroarchitecture Studies

Simone Chiola¹, Manuela Santo¹, Antonello Mallamaci¹

¹Laboratory of Cerebral Cortex Development, SISSA

Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells

Takahiro Yamakawa¹, Keiichi Itakura¹

¹Department of Molecular and Cellular Biology, Beckman Research Institute of City of Hope

Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting

Ana Korosec*¹, Sophie Frech*¹, Beate M. Lichtenberger¹

¹Skin, Endothelium Research Division, Department of Dermatology, Medical University of Vienna

Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses

Yuyang Wang*¹, Weidi Xu*^1,2, Huancheng Guo¹, Wenjie Gong¹, Biao He¹, Zhongzhong Tu¹, Changchun Tu^1,3, Ye Feng¹

¹Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, ²Jilin Agricultural University, ³Jiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious Diseases and Zoonoses

Methods Collection

Screening Cotton Genotypes for Reniform Nematode Resistance

John E. Erpelding¹, Salliana R. Stetina¹

¹Crop Genetics Research Unit, Agricultural Research Service, United States Department of Agriculture

Use of Single Molecule Fluorescent In Situ Hybridization (SM-FISH) to Quantify and Localize mRNAs in Murine Oocytes

Kelsey R. Timme¹, Jennifer R. Wood¹

¹Department of Animal Science, University of Nebraska-Lincoln

Manipulation of Gene Function in Mexican Cavefish

Bethany A. Stahl^1,2, James B. Jaggard^1,2, Jacqueline S.R. Chin^1,2, Johanna E. Kowalko^2,3, Alex C. Keene^1,2, Erik R. Duboué^2,3

¹Department of Biological Sciences, Florida Atlantic University, ²Jupiter Life Science Initiative, Florida Atlantic University, ³Harriet L. Wilkes Honors College, Florida Atlantic University

Methods Collection

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Vadir López-Salmerón*¹, Ann-Kathrin Schürholz*², Zhenni Li², Theresa Schlamp¹, Christian Wenzl³, Jan U. Lohmann³, Thomas Greb¹, Sebastian Wolf²

¹Department of Developmental Physiology, Centre for Organismal Studies (COS) Heidelberg, ²Department of Cell Biology, Centre for Organismal Studies (COS) Heidelberg, ³Department of Stem Cell Biology, Centre for Organismal Studies (COS) Heidelberg

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

Marie A. Brunet^1,2, Xavier Roucou^1,2

¹Department of Biochemistry, Université de Sherbrooke, ²PROTEO, Quebec Network for Research on Protein Function, Structure, and Engineering

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Kaiwen Ivy Liu¹, Norfala-Aliah Binte Sutrisnoh¹, Yuanming Wang^1,2, Meng How Tan^1,2

¹Genome Institute of Singapore, Agency for Science Technology and Research, ²School of Chemical and Biomedical Engineering, Nanyang Technological University

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