JoVE Journal

Genetics

JoVE Genetics includes experimental approaches to study gene function, gene expression and regulation, epigenetics, genetic disorders, population genetics, and evolution.

‹1…6 7 168 VIDEO ARTICLES

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Nicolaas H. Fourie¹, Ralph M. Peace^1,2, Sarah K. Abey¹, LeeAnne B. Sherwin¹, John W. Wiley³, Wendy A. Henderson¹

¹Digestive Disorders Unit, National Institute of Nursing Research, National Institutes of Health, DHHS, ²National Institutes of Health Research Scholar, Howard Hughes Medical Institute, ³Internal Medicine, Medical School, University of Michigan

Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans

Kentaro Noma¹, Yishi Jin^1,2

¹Division of Biological Sciences, Section of Neurobiology, University of California in San Diego and Howard Hughes Medical Institute, ²Department of Cellular and Molecular Medicine, University of California in San Diego School of Medicine and Howard Hughes Medical Institute

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Volodymyr Petrenko*¹, Camille Saini*², Laurent Perrin*¹, Charna Dibner¹

¹Department of Medical Specialties, Division of Endocrinology, Diabetes, Hypertension and Nutrition, Diabetes Center, University of Geneva Medical School, Institute of Genetics and Genomics in Geneva (iGE3), ²Population Epidemiology Unit (UEP), Community Medicine, Geneva University Hospital

Tools to Study the Role of Architectural Protein HMGB1 in the Processing of Helix Distorting, Site-specific DNA Interstrand Crosslinks

Anirban Mukherjee¹, Karen M. Vasquez¹

¹Division of Pharmacology and Toxicology, College of Pharmacy, The University of Texas at Austin

Methyl-binding DNA capture Sequencing for Patient Tissues

Rohit R. Jadhav¹, Yao V. Wang¹, Ya-Ting Hsu¹, Joseph Liu¹, Dawn Garcia², Zhao Lai², Tim H. M. Huang¹, Victor X. Jin¹

¹Department of Molecular Medicine, University of Texas Health Science Center at San Antonio, ²Greehey Children's Cancer Research Institute, University of Texas Health Science Center at San Antonio

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP)

Andreas E. Zautner¹, Raimond Lugert¹, Wycliffe O. Masanta¹, Michael Weig¹, Uwe Groß¹, Oliver Bader¹

¹Institut für Medizinische Mikrobiologie, Universitätsmedizin Göttingen

Monitoring Spatial Segregation in Surface Colonizing Microbial Populations

Theresa Hölscher¹, Anna Dragoš¹, Ramses Gallegos-Monterrosa¹, Marivic Martin¹, Eisha Mhatre¹, Anne Richter¹, Ákos T. Kovács¹

¹Terrestrial Biofilms Group, Institute of Microbiology, Friedrich Schiller University, Jena

In Situ Labeling of Mitochondrial DNA Replication in Drosophila Adult Ovaries by EdU Staining

Zhe Chen¹, Hong Xu¹

¹Lab of Molecular Genetics, National Heart, Lung and Blood Institute, National Institutes of Health

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

Keren B. Turton¹, Stephane Esnault², Larissa P. Delain², Deane F. Mosher^1,2

¹Department of Biomolecular Chemistry, University of Wisconsin-Madison, ²Department of Medicine, University of Wisconsin-Madison

The Use of Induced Somatic Sector Analysis (ISSA) for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development

Antanas Spokevicius¹, Lynette Taylor¹, Emma Melder¹, Kim Van Beveren¹, Josquin Tibbits², Nicky Creux^3,4, Gerd Bossinger¹

¹School of Ecosystem and Forest Sciences, Faculty of Science, The University of Melbourne, ²Victorian AgriBiosciences Centre, La Trobe University R&D Park, ³College of Biological Sciences, Department of Plant Biology, University of California, Davis, ⁴Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria

Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes

Yanina S. Bogliotti¹, Marcela Vilarino¹, Pablo J. Ross¹

¹Department of Animal Science, University of California, Davis

The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics

Silvia Dal Santo*¹, Mauro Commisso*¹, Erica D'Incà¹, Andrea Anesi^1,2, Matteo Stocchero³, Sara Zenoni¹, Stefania Ceoldo¹, Giovanni B. Tornielli¹, Mario Pezzotti¹, Flavia Guzzo¹

¹Biotechnology Department, University of Verona, ²Lab. of Bioorganic Chemistry, Physics Department, University of Trento, ³S-IN Soluzioni Informatiche

Delivery of Nucleic Acids through Embryo Microinjection in the Worldwide Agricultural Pest Insect, Ceratitis capitata

Paolo Gabrieli¹, Francesca Scolari¹

¹Department of Biology and Biotechnology, University of Pavia

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions

Kristin Bösch*^1,2, Lamprinos Frantzeskakis*¹, Miroslav Vraneš³, Jörg Kämper³, Kerstin Schipper^1,2, Vera Göhre^1,2,4

¹Institute for Microbiology, Heinrich-Heine University Düsseldorf, ²Bioeconomy Science Center (BioSC), ³Department of Genetics, Institute of Applied Biosciences, Karlsruhe Institute of Technology, ⁴Cluster of Excellence in Plant Sciences (CEPLAS), Heinrich-Heine University Düsseldorf

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Nitzan Samra^1,2, Yoav Arava¹

¹Faculty of Biology, Technion - Israel Institute of Technology, ²Department of Biomolecular Sciences, Weizmann Institute of Science

Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection

Po C. Liu¹, Yi T. Lee¹, Chun Y. Wang¹, Ya-Tang Yang¹

¹Electrical Engineering, National Tsing Hua University

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

Szu-I Yeh¹, Wei-Feng Fang¹, Chao-Jyun Huang¹, Tzu-Ming Wang¹, Jing-Tang Yang¹

¹Department of Mechanical Engineering, National Taiwan University

A Robotic Platform for High-throughput Protoplast Isolation and Transformation

Elizabeth M. Dlugosz¹, Scott C. Lenaghan^2,3, C. Neal Stewart, Jr.¹

¹Department of Plant Sciences, University of Tennessee, Knoxville, ²Center for Renewable Carbon, University of Tennessee, Knoxville, ³Department of Mechanical, Aerospace, and Biomedical Engineering, University of Tennessee, Knoxville

RNA Interference-based Investigation of the Function of Heat Shock Protein 27 during Corneal Epithelial Wound Healing

Aeri Yoo*¹, Hyun-Min Park*², Soon-Suk Kang², Eun-Soon Kim², Hungwon Tchah², Jae Yong Kim²

¹Department of Ophthalmology, Saevit Eye Hospital, ²Department of Ophthalmology, University of Ulsan College of Medicine, Asan Medical Center

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Ai-Qun Yu^1,2, Nina Pratomo^1,2, Tee-Kheang Ng^1,2, Hua Ling^1,2, Han-Saem Cho^1,2, Susanna Su Jan Leong^1,2,3, Matthew Wook Chang^1,2

¹Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, ²NUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), Life Sciences Institute, National University of Singapore, ³Food Science and Chemical Engineering, Singapore Institute of Technology

siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts

Adjimon Gatien Lokossou¹, Chirine Toufaily², Amandine Vargas³, Benoit Barbeau¹

¹Department of Biological Sciences, University of Quebec in Montreal, ²Department of Pharmacology and Therapeutics, McGill University, ³Department of Clinical Sciences, University of Montreal

In Vitro Transcription Assays and Their Application in Drug Discovery

Xiao Yang¹, Cong Ma^1,2

¹School of Environmental and Life Sciences, University of Newcastle, ²Department of Applied Biology and Chemical Technology, The State Key Laboratory of Chirosciences, The Hong Kong Polytechnic University

Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat

Haeyoung Jeong*¹, Sang J. Lee*², Pil Kim³

¹Super-Bacteria Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), ²Microbiomics and Immunity Research Center, Korea Research Institute of Bioscience and Bioengineering (KRIBB), ³Department of Biotechnology, The Catholic University of Korea

Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry

Hajime Miyaguchi¹

¹National Research Institute of Police Science

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