JoVE Journal
Genetics
Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations
1Unit for Lymphopoieseis, Immunology Department, INSERM U1223, University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Institut Pasteur, 2Center for Translational Science, Institut Pasteur, INSERM UMS20
Genetics
From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
1Department of Pharmaceutical Biology and Biotechnology, Albert-Ludwigs-Universität Freiburg, Germany, 2Department of Chemistry and Biology, Universität Siegen
Genetics
Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice
1Division of Radiation Health, Department of Pharmaceutical Sciences, College of Pharmacy, University of Arkansas for Medical Sciences, 2Department of Environmental Health, Fay W. Boozman School of Public Health, University of Arkansas for Medical Sciences, 3Surgical Service, Central Arkansas Veterans Healthcare System
Genetics
Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation
1Department of Chemistry, York University, 2The Centre for Research on Biomolecular Interactions, York University
Genetics
Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions
1Department of Molecular and Cellular Biology, University of Guelph
Genetics
Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
1Entomology Department, University of Maryland, 2Program in Molecular and Cell Biology, University of Maryland
Genetics
Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System
1Department of Occupational and Environmental Health, University of Arkansas for Medical Sciences, 2Department of Environmental Health Sciences, University of Alabama at Birmingham, 3Division of Radiation Health, University of Arkansas for Medical Sciences
Genetics
Measurement of Differentially Methylated INS DNA Species in Human Serum Samples as a Biomarker of Islet β Cell Death
1Department of Pediatrics, IU Center for Diabetes and Metabolic Disease, Indiana University School of Medicine, 2Department of Pediartics, Omaha Children's Hospital and Medical Center, University of Nebraska Medical Center, 3Departments of Biochemistry and Molecular Biology, Medicine, and Cellular and Integrative Physiology, IU Center for Diabetes and Metabolic Disease, Indiana University School of Medicine, 4Indiana Biosciences Research Institute
Genetics
Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts
1Department of Cardiology, Boston Children's Hospital, 2Department of Pediatrics, Harvard Medical School, 3Department of Genetics, Harvard Medical School, 4Howard Hughes Medical Institute
Genetics
Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants
1Enzyme Research Team, RIKEN Center for Sustainable Resource Science
Genetics
High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii
1Laboratory of Cell Cycle Genetics, The Rockefeller University, 2Sainsbury Laboratory, University of Cambridge
Genetics
Isolation of Giant Lampbrush Chromosomes from Living Oocytes of Frogs and Salamanders
1Department of Embryology, Carnegie Institution for Science
Genetics
Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
1Digestive Disorders Unit, National Institute of Nursing Research, National Institutes of Health, DHHS, 2National Institutes of Health Research Scholar, Howard Hughes Medical Institute, 3Internal Medicine, Medical School, University of Michigan
Genetics
Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans
1Division of Biological Sciences, Section of Neurobiology, University of California in San Diego and Howard Hughes Medical Institute, 2Department of Cellular and Molecular Medicine, University of California in San Diego School of Medicine and Howard Hughes Medical Institute
Genetics
Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
1Department of Medical Specialties, Division of Endocrinology, Diabetes, Hypertension and Nutrition, Diabetes Center, University of Geneva Medical School, Institute of Genetics and Genomics in Geneva (iGE3), 2Population Epidemiology Unit (UEP), Community Medicine, Geneva University Hospital
Genetics
Tools to Study the Role of Architectural Protein HMGB1 in the Processing of Helix Distorting, Site-specific DNA Interstrand Crosslinks
1Division of Pharmacology and Toxicology, College of Pharmacy, The University of Texas at Austin
Genetics
Methyl-binding DNA capture Sequencing for Patient Tissues
1Department of Molecular Medicine, University of Texas Health Science Center at San Antonio, 2Greehey Children's Cancer Research Institute, University of Texas Health Science Center at San Antonio
Genetics
Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP)
1Institut für Medizinische Mikrobiologie, Universitätsmedizin Göttingen
Genetics
Monitoring Spatial Segregation in Surface Colonizing Microbial Populations
1Terrestrial Biofilms Group, Institute of Microbiology, Friedrich Schiller University, Jena
Genetics
In Situ Labeling of Mitochondrial DNA Replication in Drosophila Adult Ovaries by EdU Staining
1Lab of Molecular Genetics, National Heart, Lung and Blood Institute, National Institutes of Health
Genetics
Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts
1Department of Biomolecular Chemistry, University of Wisconsin-Madison, 2Department of Medicine, University of Wisconsin-Madison
Genetics
The Use of Induced Somatic Sector Analysis (ISSA) for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development
1School of Ecosystem and Forest Sciences, Faculty of Science, The University of Melbourne, 2Victorian AgriBiosciences Centre, La Trobe University R&D Park, 3College of Biological Sciences, Department of Plant Biology, University of California, Davis, 4Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria
Genetics
Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes
1Department of Animal Science, University of California, Davis
Genetics
