JoVE Journal
Genetics
Pooled shRNA Screen for Reactivation of MeCP2 on the Inactive X Chromosome
1Clinical Research Division, Fred Hutchinson Cancer Research Center, 2Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 3Epigenetics Program, Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, 4Departments of Medicine and Biochemistry, University of Washington
Genetics
Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay
1Department of Biotechnology and Animal Science, National Ilan University, 2Department of Agricultural Biology, College of Agriculture Life Sciences, Chonbuk National University, 3Department of Entomology, National Taiwan University
Genetics
Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection
1Department of Medicine II, Klinikum rechts der Isar, Technische Universität München, 2Department of Pneumology, Center for Medicine, Medical Center University of Freiburg
Genetics
Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
1Department of Biological Chemistry, School of Medicine, University of California, Irvine, 2Beckman Laser Institute and Medical Clinic, University of California, Irvine, 3Department of Developmental and Cell Biology, School of Biological Sciences, University of California, Irvine, 4Department of Biomedical Engineering and Surgery, University of California, Irvine
Genetics
Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA
1Genetic and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU), 2Department of Industrial and Environmental Biotechnology, National Institute of Genetics Engineering and Biotechnology (NIGEB), 3Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences & Natural Resources, 4RG Abiotic Stress Genomics/ RG Heterosis, Department Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)
Genetics
Microinjection of CRISPR/Cas9 Protein into Channel Catfish, Ictalurus punctatus, Embryos for Gene Editing
1School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, 2Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University, 3Anatomy and Embryology Department, Faculty of Veterinary Medicine, Cairo University, 4Department of Molecular Physiology and Biophysics, Vanderbilt University, 5Life Science Institute, University of Michigan
Genetics
Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts
1Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, 2Department of Biological Chemistry, David Geffen School of Medicine, 3Molecular Biology Institute Interdepartmental Program, University of California, Los Angeles
Genetics
Embryo Microinjection and Transplantation Technique for Nasonia vitripennis Genome Manipulation
1Department of Entomology and Riverside Center of Disease Vector Research, Institute for Integrative Genome Biology, University of California, Riverside, 2Section of Cell and Developmental Biology, Division of Biological Sciences, University of California, San Diego
Genetics
Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis
1Department of Microbiology and Immunology, Michael Smith Laboratories Centre for High-Throughput Biology, University of British Columbia, 2Canada's Michael Smith Genome Science Center, BC Cancer Agency
Genetics
A Universal Protocol for Large-scale gRNA Library Production from any DNA Source
1MCN Junior Research Group, Munich Center for Neurosciences, Ludwig-Maximilian-Universität, BioMedical Center, 2Epigenetic Engineering, Institute of Stem Cell Research, Helmholtz Zentrum, German Research Center for Environmental Health
Genetics
An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants
1Laboratory of Plant Genetics and Development, Noble Research Institute, 2Genetics Laboratory, University of Oklahoma Health Science Center, 3Medicine and Health School, Li Shui University
Genetics
Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning
1Department of Translational Research, Tsurumi University School of Dental Medicine, 2Endowed Department of International Oral Health Science (affiliated with Department of Translational Research), Tsurumi University School of Dental Medicine, 3Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University
Genetics
In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells
1Department of Environmental and Occupational Health, School of Public Health, NIEHS Center for Environmental Exposures and Disease, Environmental and Occupational Health Sciences Institute, Rutgers University, 2Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency
Genetics
