JoVE Journal

Genetics

JoVE Genetics includes experimental approaches to study gene function, gene expression and regulation, epigenetics, genetic disorders, population genetics, and evolution.

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Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes

Yanina S. Bogliotti¹, Marcela Vilarino¹, Pablo J. Ross¹

¹Department of Animal Science, University of California, Davis

The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics

Silvia Dal Santo*¹, Mauro Commisso*¹, Erica D'Incà¹, Andrea Anesi^1,2, Matteo Stocchero³, Sara Zenoni¹, Stefania Ceoldo¹, Giovanni B. Tornielli¹, Mario Pezzotti¹, Flavia Guzzo¹

¹Biotechnology Department, University of Verona, ²Lab. of Bioorganic Chemistry, Physics Department, University of Trento, ³S-IN Soluzioni Informatiche

Delivery of Nucleic Acids through Embryo Microinjection in the Worldwide Agricultural Pest Insect, Ceratitis capitata

Paolo Gabrieli¹, Francesca Scolari¹

¹Department of Biology and Biotechnology, University of Pavia

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions

Kristin Bösch*^1,2, Lamprinos Frantzeskakis*¹, Miroslav Vraneš³, Jörg Kämper³, Kerstin Schipper^1,2, Vera Göhre^1,2,4

¹Institute for Microbiology, Heinrich-Heine University Düsseldorf, ²Bioeconomy Science Center (BioSC), ³Department of Genetics, Institute of Applied Biosciences, Karlsruhe Institute of Technology, ⁴Cluster of Excellence in Plant Sciences (CEPLAS), Heinrich-Heine University Düsseldorf

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Nitzan Samra^1,2, Yoav Arava¹

¹Faculty of Biology, Technion - Israel Institute of Technology, ²Department of Biomolecular Sciences, Weizmann Institute of Science

Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection

Po C. Liu¹, Yi T. Lee¹, Chun Y. Wang¹, Ya-Tang Yang¹

¹Electrical Engineering, National Tsing Hua University

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

Szu-I Yeh¹, Wei-Feng Fang¹, Chao-Jyun Huang¹, Tzu-Ming Wang¹, Jing-Tang Yang¹

¹Department of Mechanical Engineering, National Taiwan University

A Robotic Platform for High-throughput Protoplast Isolation and Transformation

Elizabeth M. Dlugosz¹, Scott C. Lenaghan^2,3, C. Neal Stewart, Jr.¹

¹Department of Plant Sciences, University of Tennessee, Knoxville, ²Center for Renewable Carbon, University of Tennessee, Knoxville, ³Department of Mechanical, Aerospace, and Biomedical Engineering, University of Tennessee, Knoxville

RNA Interference-based Investigation of the Function of Heat Shock Protein 27 during Corneal Epithelial Wound Healing

Aeri Yoo*¹, Hyun-Min Park*², Soon-Suk Kang², Eun-Soon Kim², Hungwon Tchah², Jae Yong Kim²

¹Department of Ophthalmology, Saevit Eye Hospital, ²Department of Ophthalmology, University of Ulsan College of Medicine, Asan Medical Center

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Ai-Qun Yu^1,2, Nina Pratomo^1,2, Tee-Kheang Ng^1,2, Hua Ling^1,2, Han-Saem Cho^1,2, Susanna Su Jan Leong^1,2,3, Matthew Wook Chang^1,2

¹Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, ²NUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), Life Sciences Institute, National University of Singapore, ³Food Science and Chemical Engineering, Singapore Institute of Technology

siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts

Adjimon Gatien Lokossou¹, Chirine Toufaily², Amandine Vargas³, Benoit Barbeau¹

¹Department of Biological Sciences, University of Quebec in Montreal, ²Department of Pharmacology and Therapeutics, McGill University, ³Department of Clinical Sciences, University of Montreal

In Vitro Transcription Assays and Their Application in Drug Discovery

Xiao Yang¹, Cong Ma^1,2

¹School of Environmental and Life Sciences, University of Newcastle, ²Department of Applied Biology and Chemical Technology, The State Key Laboratory of Chirosciences, The Hong Kong Polytechnic University

Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat

Haeyoung Jeong*¹, Sang J. Lee*², Pil Kim³

¹Super-Bacteria Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), ²Microbiomics and Immunity Research Center, Korea Research Institute of Bioscience and Bioengineering (KRIBB), ³Department of Biotechnology, The Catholic University of Korea

Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry

Hajime Miyaguchi¹

¹National Research Institute of Police Science

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