JoVE Journal

Immunology and Infection

JoVE Immunology and Infection includes methods and techniques for studying the immune system, mechanisms of infection, biological responses to pathogens, and therapeutic agents for treating immunological disease.

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Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4⁺ T Cells Isolated from Rhesus macaques

Andrey Tokarev¹, Matthew Creegan¹, Michael A. Eller¹, Mario Roederer², Diane L. Bolton¹

¹US Military HIV Research Program, Henry M. Jackson Foundation, Walter Reed Army Institute of Research, ²Vaccine Research Center, NIAID, NIH

Dynamic Adhesion Assay for the Functional Analysis of Anti-adhesion Therapies in Inflammatory Bowel Disease

Emily Becker¹, Sebastian Schramm¹, Marie-Theres Binder¹, Clarissa Allner¹, Maximilian Wiendl¹, Clemens Neufert¹, Imke Atreya¹, Markus Neurath¹, Sebastian Zundler¹

¹Department of Medicine 1, University of Erlangen-Nuremberg

Immunostimulatory Agent Evaluation: Lymphoid Tissue Extraction and Injection Route-Dependent Dendritic Cell Activation

Jun-O Jin^1,2, Soyeong Jang³, Hyehyun Kim⁴, Junghwan Oh⁴, Sungbo Shim⁵, Minseok Kwak^3,4, Peter C.W. Lee⁶

¹Shanghai Public Health Clinical Center, Shanghai Medical College, Fudan University, ²Department of Medical Biotechnology, Yeungnam University, ³Department of Chemistry, Pukyong National University, ⁴Marine-integrated Bionics Research Center, Pukyong National University, ⁵Department of Biochemistry, Chungbuk National University, ⁶Department of Biomedical Sciences, University of Ulsan College of Medicine, Asan Medical Center

Mouse Body Temperature Measurement Using Infrared Thermometer During Passive Systemic Anaphylaxis and Food Allergy Evaluation

Yu Kawakami¹, Rachel Sielski¹, Toshiaki Kawakami¹

¹Division of Cell Biology, La Jolla Institute for Allergy and Immunology

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors

Emily A. Rex¹, Dahee Seo¹, Don B. Gammon¹

¹Department of Microbiology, University of Texas Southwestern Medical Center

Visual and Microscopic Evaluation of Streptomyces Developmental Mutants

Jennifer A. Bennett¹, Garrett V. Kandell¹, Sean G. Kirk¹, Joseph R. McCormick²

¹Department of Biology and Earth Science, Biochemistry and Molecular Biology Program, Otterbein University, ²Department of Biological Sciences, Duquesne University

In Vitro Differentiation of Mouse Granulocyte-macrophage-colony-stimulating Factor (GM-CSF)-producing T Helper (T_HGM) Cells

Yi Lu^1,2,3, Xin-Yuan Fu³, Yongliang Zhang^1,2

¹Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, ²Immunology Programme, Life Sciences Institute, National University of Singapore, ³Cancer Science Institute of Singapore, Yong Loo Lin School of Medicine, National University of Singapore

Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay

Ruby Cheung¹, Gregory W. deHart¹, Lynne Jesaitis¹, Stephen J. Zoog¹, Andrew C. Melton¹

¹BioMarin Pharmaceutical Inc

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Almin I. Lalani¹, Sining Zhu¹, Ping Xie^2,3

¹Department of Cell Biology and Neuroscience and Graduate Program in Cellular and Molecular Pharmacology, Rutgers University, ²Department of Cell Biology and Neuroscience, Rutgers University, ³Rutgers Cancer Institute of New Jersey

Stereotactic Adoptive Transfer of Cytotoxic Immune Cells in Murine Models of Orthotopic Human Glioblastoma Multiforme Xenografts

Ulrich Jarry*^1,2, Noémie Joalland*^1,2, Cynthia Chauvin*^1,2, Béatrice Clemenceau^1,2,3, Claire Pecqueur^1,2, Emmanuel Scotet^1,2

¹INSERM, CNRS, Université d'Angers, Université de Nantes, CRCINA, ²Immunotherapy, Graft, Oncology, LabEx IGO, ³Hopital de Nantes, Hotel Dieu

In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy

Ronald H.L. Li¹, Fern Tablin²

¹Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, ²Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California, Davis

Isolation and Identification of Extravascular Immune Cells of the Heart

Laura Aronoff^1,2, Slava Epelman^1,2,3,4,5, Xavier Clemente-Casares^1,2

¹Toronto General Hospital Research Institute, University Health Network (UHN), ²Dept of Laboratory Medicine and Pathobiology, University of Toronto, ³Dept of Immunology, University of Toronto, ⁴Peter Munk Cardiac Centre, ⁵Ted Rogers Centre for Heart Research

An In Vivo Mouse Model to Measure Naïve CD4 T Cell Activation, Proliferation and Th1 Differentiation Induced by Bone Marrow-derived Dendritic Cells

Raquel Toribio-Fernandez¹, Virginia Zorita¹, Beatriz Herrero-Fernandez², Jose M. Gonzalez-Granado^1,2,3

¹LamImSys Lab, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), ²LamImSys Lab, Instituto de Investigación Sanitaria Hospital 12 de Octubre (imas12), ³CIBER de Enfermedades Cardiovasculares

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy

Kyle Taruc¹, Charles Yin¹, Daniel G. Wootton^2,3, Bryan Heit¹

¹Department of Microbiology and Immunology and the Center for Human Immunology, University of Western Ontario, ²Institute of Infection and Global Health, University of Liverpool, ³Department of Respiratory Research, Aintree University Hospital NHS Foundation Trust

Imaging Cell Interaction in Tracheal Mucosa During Influenza Virus Infection Using Two-photon Intravital Microscopy

Miguel Palomino-Segura*^1,2, Tommaso Virgilio*^1,2, Diego Morone¹, Diego U. Pizzagalli^1,3, Santiago F. Gonzalez¹

¹Faculty of Biomedical Sciences, Institute for Research in Biomedicine, Università della Svizzera italiana (USI), ²Graduate School of Cellular and Molecular Sciences, Faculty of Medicine, University of Bern, ³Institute of Computational Science, Università della Svizzera italiana (USI)

A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion

Anthony M. Esposito^1,2, Alexandra Y. Soare¹, Foramben Patel¹, Namita Satija¹, Benjamin K. Chen¹, Talia H. Swartz¹

¹Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, Immunology Institute, ²Department of Biology, New Jersey City University

Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin

Dechun Zhang*¹, Xianzhao Kan*², Sarah Elizabeth Huss³, Lan Jiang², Li-Qing Chen³, Yibing Hu⁴

¹Key Laboratory of Three Gorges Regional Plant Genetics and Germplasm Enhancement (CTGU)/Biotechnology Research Center, China Three Gorges University, ²The Institute of Bioinformatics, College of Life Sciences, Anhui Normal University, ³Department of Plant Biology, University of Illinois at Urbana-Champaign, ⁴College of Resources & Environmental Sciences, Nanjing Agricultural University

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo

Line L. Holm^1,2,3, Milica Vukmanovic-Stejic⁴, Thomas Blauenfeldt¹, Thomas Benfield^2,3, Peter Andersen¹, Arne N. Akbar⁴, Morten Ruhwald¹

¹Department of Infectious Disease Immunology, Center for Vaccine Research, Statens Serum Institut, ²Department of Infectious Diseases, Hvidovre Hospital, ³Institute of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, ⁴Division of Infection and Immunity, University College London

Generation of Large Numbers of Myeloid Progenitors and Dendritic Cell Precursors from Murine Bone Marrow Using a Novel Cell Sorting Strategy

Peter B. Rogers¹, Elizabeth Hiltbold Schwartz¹

¹Department of Biological Sciences, Auburn University

Assessment of the Synaptic Interface of Primary Human T Cells from Peripheral Blood and Lymphoid Tissue

Maria Steblyanko¹, Nadia Anikeeva¹, Marcus Buggert^2,3, Michael R. Betts², Yuri Sykulev⁴

¹Department of Microbiology and Immunology, Thomas Jefferson University, ²Department of Microbiology and Institute for Immunology, Perelman School of Medicine, University of Pennsylvania, ³Department of Medicine Huddinge, Karolinska Institutet, Karolinska University Hospital Huddinge, ⁴Departments of Microbiology and Immunology and Medical Oncology, Sidney Kimmel Cancer Center, Thomas Jefferson University

Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow

Alberto Yáñez^1,2, Helen S. Goodridge^1,2

¹Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, ²Research Division of Immunology, Cedars-Sinai Medical Center

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

Jeanmarie Verchot¹, Aastha Thapa², Dulanjani Wijayasekara³, Peter R. Hoyt⁴

¹Texas A&M Agrilife Center at Dallas, ²Noble Research Center, Oklahoma State University, ³Department of Biology, College of Engineering and Natural Sciences, The University of Tulsa, ⁴Bioinformatics and Genomics Core Facility, Department of Biochemistry and Molecular Biology, Oklahoma State University

Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue

Bronson A. Haynes¹, Ryan W. Huyck¹, Ashley J. James¹, Meghan E. Carter¹, Omnia U. Gaafar¹, Marjorie Day¹, Avennette Pinto¹, Anca D. Dobrian¹

¹Department of Physiological Sciences, Eastern Virginia Medical School

Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens

Irati Romero-Garmendia*¹, Amaia Jauregi-Miguel*¹, Izortze Santin², Jose Ramón Bilbao¹, Ainara Castellanos-Rubio¹

¹Department of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country (UPV-EHU), Biocruces Health Research Institute, ²Endocrinology and Diabetes Research Group, Biocruces Health Research Institute, UPV-EHU, CIBERDEM

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