JoVE Journal

Immunology and Infection

JoVE Immunology and Infection includes methods and techniques for studying the immune system, mechanisms of infection, biological responses to pathogens, and therapeutic agents for treating immunological disease.

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Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate

Roy Al Ahmar*¹, Brandon D. Kirby*², Hongwei D. Yu^1,2,3

¹Department of Biomedical Sciences, Joan C. Edwards School of Medicine, Marshall University, ²Progenesis Technologies LLC, Robert C. Byrd Biotechnology Science Center, ³Department of Pediatrics, Joan C. Edwards School of Medicine, Marshall University

In Vivo Infection with Leishmania amazonensis to Evaluate Parasite Virulence in Mice

Juliana Ide Aoki¹, Ahyun Hong¹, Ricardo Andrade Zampieri¹, Lucile Maria Floeter-Winter¹, Maria Fernanda Laranjeira-Silva¹

¹Department of Physiology, Institute of Bioscience, University of Sao Paulo

A Delayed Inoculation Model of Chronic Pseudomonas aeruginosa Wound Infection

Christiaan R. de Vries*¹, Johanna M. Sweere*^1,2, Heather Ishak^1,3, Vivekananda Sunkari¹, Michelle S. Bach¹, Dan Liu¹, Robert Manasherob¹, Paul L. Bollyky^1,2

¹Division of Infectious Diseases, School of Medicine, Stanford University, ²Stanford Immunology, Stanford University, ³Palo Alto Veterans Institute of Research

Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR

Suman K. Pradhan¹, Jerry Guzman¹, Carl Dargitz¹, Stephanie Switalski², Mark Landon¹, Sven Olek³, Bjoern Samans³, Ulrich Hoffmueler³, Uma Lakshmipathy¹

¹Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, ²California Polytechnic State University (Cal Poly), ³Epiontis GmbH, Precision for Medicine

Targeting Drugs to Larval Zebrafish Macrophages by Injecting Drug-Loaded Liposomes

Tanja Linnerz¹, Manju Kanamala², Jonathan W. Astin¹, Nicola Dalbeth³, Zimei Wu², Christopher J. Hall¹

¹Department of Molecular Medicine and Pathology, Faculty of Medical and Health Sciences, University of Auckland, ²School of Pharmacy, Faculty of Medical and Health Sciences, University of Auckland, ³School of Medicine, Faculty of Medical and Health Sciences, University of Auckland

Analyzing the Permeability of the Blood-Brain Barrier by Microbial Traversal through Microvascular Endothelial Cells

Veronika Weber¹, Kaya Bork¹, Rüdiger Horstkorte¹, Heidi Olzscha¹

¹Institute of Physiological Chemistry, Martin Luther University Halle-Wittenberg

Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR

Fei-Xue Ban¹, Tian-Yan Yin¹, Qi Guo¹, Li-Long Pan¹, Yin-Quan Liu¹, Xiao-Wei Wang¹

¹Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogen and Insects, Institute of Insect Sciences, Zhejiang University

Humanized NOG Mice for Intravaginal HIV Exposure and Treatment of HIV Infection

Anna H. F. Andersen^1,2, Stine S. F. Nielsen^1,2, Rikke Olesen^1,2, Katharina Mack¹, Frederik Dagnæs-Hansen³, Niels Uldbjerg^1,4, Lars Østergaard^1,2, Ole S. Søgaard^1,2, Paul W. Denton^1,2,5, Martin Tolstrup^1,2

¹Department of Clinical Medicine, Aarhus University, ²Department of Infectious Diseases, Aarhus University Hospital, ³Department of Biomedicine, Aarhus University, ⁴Department of Gynecology and Obstetrics, Aarhus University Hospital, ⁵Department of Biology, University of Nebraska at Omaha

Adoptive Immunotherapy of iNKT Cells in Glucose-6-Phosphate Isomerase (G6PI)-Induced RA Mice

Ming Meng*^1,2, Shengde Chen*^1,2, Xiang Gao*^1,2, Huifang Liu*^1,2, Yuanyuan Wang*^1,2, Jingnan Zhang*^1,2, Haiyang Dou*^1,2, Wenjuan Li*^1,2, Dongzhi Chen*^1,2

¹Medical School of Hebei University, ²Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-Autoimmune Diseases in Hebei Province

Preparation and Utilization of Freshly Isolated Human Detrusor Smooth Muscle Cells for Characterization of 9-Phenanthrol-Sensitive Cation Currents

John Malysz¹, Eric S. Rovner², Robert Wake³, Georgi V. Petkov^1,3,4

¹Department of Pharmaceutical Sciences, College of Pharmacy, University of Tennessee Health Science Center, ²Department of Urology, Medical University of South Carolina, ³Department of Urology, College of Medicine, University of Tennessee Health Science Center, ⁴Department of Pharmacology, College of Medicine, University of Tennessee Health Science Center

Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis

Gabrielle Keb¹, Kenneth A. Fields¹

¹Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky

Monitoring Extracellular pH in Cross-Kingdom Biofilms using Confocal Microscopy

Sebastian Schlafer¹, Mathilde Frost Kristensen¹

¹Department of Dentistry and Oral Health, Aarhus University

In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction

Hongzheng Bi^1,2, Samiksha Wasnik¹, David J. Baylink¹, Chenfan Liu^1,3,4, Xiaolei Tang^1,3

¹Division of Regenerative Medicine, Department of Medicine, Loma Linda University, ²Zhengzhou University, ³Department of Veterinary Biomedical Sciences, College of Veterinary Medicine, Long Island University, ⁴Jinan Infectious Disease Hospital, Shandong University

Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils

Jeffrey W. Chadwick^1,2, Michael Glogauer^1,2

¹Department of Dental Oncology and Maxillofacial Prosthetics, Princess Margaret Cancer Centre, University Health Network, ²Faculty of Dentistry, University of Toronto

Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses

Fengwei Zhang¹, Yanan Wang², Xuechai Shang¹, Shanshan Wang¹, Rong Xiao¹, Hongjuan Zhou¹, Long Cai¹

¹Central Laboratory, Hang Zhou Red Cross Hospital, ²Clinical Laboratory, Yuhang District Maternity and Child Health Care Hospital

NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells

Jonathan M. Mendez¹, A. Marijke Keestra-Gounder¹

¹Department of Immunology and Microbiology, University of Colorado Anschutz Medical Campus

Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection

Meagan E. Valentine¹, Brandon D. Kirby¹, Hongwei D. Yu^1,2

¹Progenesis Technologies, LLC, ²Department of Biomedical Sciences, Pediatrics, Joan C. Edwards School of Medicine at Marshall University

Quantifying the Cytotoxicity of Staphyloccus aureus Against Human Polymorphonuclear Leukocytes

Jennifer G. Dankoff¹, Kyler B. Pallister¹, Fermin E. Guerra¹, Alexander J. Parks¹, Kelly Gorham², Saul Mastandrea², Jovanka M. Voyich¹, Tyler K. Nygaard¹

¹Department of Microbiology and Immunology, Montana State University, ²University Communications, Montana State University

Imaging the Human Immunological Synapse

Ana Bello-Gamboa*^1,2, Juan Manuel Izquierdo^1,2, Marta Velasco^1,2, Solange Moreno^1,2, Alejandro Garrido^1,2, Laura Meyers^1,2, Juan Carlos Palomino³, Víctor Calvo^1,2, Manuel Izquierdo*^1,2

¹Instituto de Investigaciones Biomédicas Alberto Sols, CSIC-Universidad Autónoma de Madrid, ²Departamento de Bioquímica, Instituto de Investigaciones Biomédicas Alberto Sols CSIC-UAM, Facultad de Medicina, Universidad Autónoma de Madrid, ³Unidad de Audiovisuales, Facultad de Medicina, Universidad Autónoma de Madrid

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

Xin Hu¹, Jaimee R. Compton², Patricia M. Legler²

¹National Center for Advancing Translational Sciences, National Institutes of Health, ²United States Naval Research Laboratory

Implantation and Monitoring by PET/CT of an Orthotopic Model of Human Pleural Mesothelioma in Athymic Mice

Didier J. Colin¹, Olivia Bejuy¹, Stéphane Germain¹, Frédéric Triponez², Véronique Serre-Beinier²

¹MicroPET/SPECT/CT Imaging Laboratory, Centre for BioMedical Imaging (CIBM), Division of Nuclear Medicine and Molecular Imaging, University Hospitals and University of Geneva, ²Division of Thoracic and Endocrine Surgery, University Hospitals and University of Geneva

Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens

Geetika Bajpai¹, Kory J. Lavine^1,2,3

¹Department of Medicine, Washington University School of Medicine, ²Department of Immunology and Pathology, Washington University School of Medicine, ³Department of Developmental Biology, Washington University School of Medicine

Isolation, Transfection, and Culture of Primary Human Monocytes

Karlie Plaisance-Bonstaff^1,2, Celeste Faia², Dorota Wyczechowska¹, Duane Jeansonne¹, Cecilia Vittori^1,3, Francesca Peruzzi^1,2

¹Department of Medicine, Louisiana State University Health Sciences Center, ²Department of Microbiology, Immunology, and Parasitology, Louisiana State University Health Sciences Center, ³Department of Biomedical and Clinical Sciences L. Sacco, University of Milan

Visualization and Quantification of High-Dimensional Cytometry Data using Cytofast and the Upstream Clustering Methods FlowSOM and Cytosplore

Guillaume Beyrend¹, Koen Stam², Ferry Ossendorp¹, Ramon Arens¹

¹Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, ²Department of Parasitology, Leiden University Medical Center

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