JoVE Journal

Immunology and Infection

JoVE Immunology and Infection includes methods and techniques for studying the immune system, mechanisms of infection, biological responses to pathogens, and therapeutic agents for treating immunological disease.

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T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

Lael Werner^1,2, Chen Dor^3,4, Naomi Salamon¹, Meital Nagar^3,4, Dror S. Shouval^1,2

¹Pediatric Gastroenterology Unit, Edmond and Lily Safra Children's Hospital, Sheba Medical Center, ²Sackler Faculty of Medicine, Tel Aviv University, ³Cancer Research Center, Sheba Medical Center, ⁴Molecular Hematology Laboratory, Sheba Medical Center

JoVE 61792

A DNA/Ki67-Based Flow Cytometry Assay for Cell Cycle Analysis of Antigen-Specific CD8 T Cells in Vaccinated Mice

Sonia Simonetti*^1,2, Ambra Natalini*^1,2, Giovanna Peruzzi³, Alfredo Nicosia⁴, Antonella Folgori⁵, Stefania Capone⁵, Angela Santoni², Francesca Di Rosa¹

¹Institute of Molecular Biology and Pathology, National Research Council of Italy (CNR), ²Department of Molecular Medicine, University of Rome “Sapienza”, ³Center for Life Nano Science, Istituto Italiano di Tecnologia, ⁴Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, ⁵Reithera Srl

JoVE 61867

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Anna Kula-Pacurar¹, Jakub Wadas², Agnieszka Suder¹, Artur Szczepanski^1,2, Aleksandra Milewska^1,2, Marek Ochman^3,4, Tomasz Stacel^3,4, Krzysztof Pyrc¹

¹Malopolska Centre of Biotechnology, Jagiellonian University, ²Microbiology Department Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, ³Department of Cardiac, Vascular and Endovascular Surgery and Transplantology, The Medical University of Silesia in Katowice, ⁴Silesian Centre for Heart Diseases

JoVE 62067

Methods Collection

Quantifying the Effects of Antimicrobials on In vitro Biofilm Architecture using COMSTAT Software

Amanda J. Morris¹, Alvin Li¹, Lindsay Jackson¹, Yvonne C. W. Yau^1,2, Valerie Waters^1,3

¹Translational Medicine, Hospital for Sick Children, ²Microbiology, Department of Pediatric Laboratory Medicine, Hospital for Sick Children, ³Infectious Diseases, Department of Pediatrics, Hospital for Sick Children

JoVE 61759

Recurrent Escherichia coli Urinary Tract Infection Triggered by Gardnerella vaginalis Bladder Exposure in Mice

Valerie P. O'Brien^1,2,6, Matthew S. Joens^3,7, Amanda L. Lewis^1,2,4,8, Nicole M. Gilbert^2,5

¹Department of Molecular Microbiology, Washington University School of Medicine in Saint Louis, ²Center for Women’s Infectious Disease Research, Washington University School of Medicine in Saint Louis, ³Center for Cellular Imaging, Washington University School of Medicine in Saint Louis, ⁴Department of Obstetrics and Gynecology, Washington University School of Medicine in Saint Louis, ⁵Department of Pediatrics, Washington University School of Medicine in Saint Louis, ⁶Fred Hutchinson Cancer Research Center, ⁷TESCAN USA, Inc., ⁸University of California San Diego

JoVE 61967

Methods Collection

Live Imaging of Chemokine Receptors in Zebrafish Neutrophils During Wound Responses

Antonios Georgantzoglou¹, Caroline Coombs¹, Hugo Poplimont*¹, Hazel A. Walker*¹, Milka Sarris¹

¹Department of Physiology, Development and Neuroscience, University of Cambridge

JoVE 61679

An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding Performance

Luz DC. Martínez-Sánchez*¹, Rashmita Pradhan*¹, Phuong A. Ngo¹, Lena Erkert¹, Lukas S. Becker¹, Alastair J. Watson², Imke Atreya*¹, Markus F. Neurath*¹, Rocío López-Posadas*¹

¹Medical Clinic 1, University of Erlangen-Nuremberg, University Hospital of Erlangen, ²Norwich Medical School, University of East Anglia, Norwich Research Park

JoVE 60790

Profiling of the Human Natural Killer Cell Receptor-Ligand Repertoire

Elena Vendrame*¹, Julia L. McKechnie*^1,2, Thanmayi Ranganath¹, Nancy Q. Zhao^1,2, Arjun Rustagi¹, Rosemary Vergara¹, Geoffrey T. Ivison^1,2, Lisa M. Kronstad¹, Laura J. Simpson¹, Catherine A. Blish^1,2,3

¹Department of Medicine, Stanford University School of Medicine, ²Program in Immunology, Stanford University School of Medicine, ³Chan-Zuckerberg BioHub

JoVE 61912

Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune Encephalomyelitis

Zhe Ji^1,2, Chenghua Zhou¹, Hongshen Niu³, Jinshen Wang¹, Lei Shen³

¹Translational Medicine Research Center, Ruijin Hospital North, Shanghai Jiao Tong University School of Medicine, ²Department of Microbiology and Immunology, Tongji University School of Medicine, ³Shanghai Institute of Immunology, Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Shanghai Jiao Tong University School of Medicine

JoVE 61050

Differentiation of Monocytes into Phenotypically Distinct Macrophages After Treatment with Human Cord Blood Stem Cell (CB-SC)-Derived Exosomes

Wei Hu^1,2, Xiang Song¹, Haibo Yu¹, Jingyu Sun², Yong Zhao¹

¹Center for Discovery and Innovation, Hackensack Meridian Health Center, ²Department of Chemistry and Chemistry Biology, Stevens Institute of Technology

JoVE 61562

Nasal Brushing Sampling and Processing using Digital High Speed Ciliary Videomicroscopy – Adaptation for the COVID-19 Pandemic

Noemie Bricmont¹, Lionel Benchimol², Anne-Lise Poirrier², Christine Grignet³, Claire Seaton⁴, Mark A. Chilvers⁴, Marie-Christine Seghaye⁵, Renaud Louis^1,6, Philippe Lefebvre², Celine Kempeneers^1,7

¹Pneumology laboratory, I3 Group, GIGA Research Center, University of Liège, ²Department of ENT, University Hospital of Liège, ³Department for Occupational Safety and Health, Biosafety and Biosecurity Unit, University of Liège, ⁴Division of Respirology, Department of Pediatrics, University of British Columbia and British Columbia Children's Hospital, ⁵Division of Cardiology, Department of Pediatrics, University Hospital of Liège, ⁶Department of Pneumology, University Hospital of Liège, ⁷Division of Respirology, Department of Pediatrics, University Hospital of Liège

JoVE 61949

A Contemporary Warming/Restraining Device for Efficient Tail Vein Injections in a Murine Fungal Sepsis Model

Junko Yano¹, Elizabeth A. Lilly², Mairi C. Noverr², Paul L. Fidel¹

¹Center for Oral and Craniofacial Biology, Louisiana State University Health-School of Dentistry, ²Department of Microbiology and Immunology, Tulane University School of Medicine

JoVE 61961

Fecal (micro) RNA Isolation

Fyonn H. Dhang^1,2, Howard L. Weiner^1,2, Shirong Liu^1,2

¹Ann Romney Center for Neurologic Diseases, Department of Neurology, Partners Multiple Sclerosis Center, Brigham and Women's Hospital and Harvard Medical School, ²Evergrande Center for Immunologic Diseases, Harvard Medical School and Brigham and Women's Hospital

JoVE 61908

Feeding and Quantifying Animal-Derived Blood and Artificial Meals in Aedes aegypti Mosquitoes

Veronica Jové*¹, Krithika Venkataraman*¹, Thomas M. Gabel², Laura B. Duvall²

¹Laboratory of Neurogenetics and Behavior, The Rockefeller University, ²Department of Biological Sciences, Columbia University

JoVE 61835

Methods Collection

Cigarette Smoke Exposure in Mice using a Whole-Body Inhalation System

Daniel E. Morales-Mantilla^1,2, Xinyan Huang^3,7, Philip Erice^1,3, Paul Porter⁴, Yun Zhang^1,5, Mary Figueroa⁶, Joya Chandra⁶, Katherine Y. King², Farrah Kheradmand^4,8, Antony Rodríguez^3,8

¹Program in Immunology, Baylor College of Medicine, ²Department of Pediatrics, Section of Infectious Diseases, Baylor College of Medicine, ³Department of Medicine - Immunology Allergy and Rheumatology, Baylor College of Medicine, ⁴Department of Medicine, Pulmonary, Critical Care, Sleep Medicine, Baylor College of Medicine, ⁵Department of Pathology and Immunology, Baylor College of Medicine, ⁶Department of Pediatrics, Research and Department of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center, ⁷Division of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Sun Yat-sen University, ⁸Center for Translational Research on Inflammatory Diseases (CTRID), Michael E. DeBakey VA Medical Center