JoVE Journal

Immunology and Infection

JoVE Immunology and Infection includes methods and techniques for studying the immune system, mechanisms of infection, biological responses to pathogens, and therapeutic agents for treating immunological disease.

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Measuring Naturally Acquired Phagocytosis-Inducing Antibodies to Plasmodium falciparum Parasites by a Flow Cytometry-Based Assay

Maria del Pilar Quintana¹, Nsoh Godwin Anabire^1,2,3, Lars Hviid^1,4

¹Centre for Medical Parasitology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, ²West African Centre for Cell Biology of Infectious Pathogens, Department of Biochemistry, Cell and Molecular Biology, University of Ghana, ³Department of Immunology, Noguchi Memorial Institute for Medical Research, ⁴Centre for Medical Parasitology, Department of Infectious Diseases, Rigshospitalet

JoVE 61538

Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions

Kaitlin D Yarrington*¹, Andrea Sánchez Peña*¹, Dominique H Limoli¹

¹Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa

JoVE 61376

Visualization of Pseudomonas aeruginosa within the Sputum of Cystic Fibrosis Patients

Lindsay Jackson¹, William DePas², Amanda J. Morris¹, Kevin Guttman¹, Yvonne C W Yau^1,3, Valerie Waters^1,4

¹Translational Medicine, Hospital for Sick Children, ²Center for Microbial Pathogenesis, Department of Pediatrics, University of Pittsburgh, ³Microbiology, Department of Pediatric Laboratory Medicine, Hospital for Sick Children, ⁴Infectious Diseases, Department of Pediatrics, Hospital for Sick Children

JoVE 61631

Neisseria meningitidis Infection of Induced Pluripotent Stem-Cell Derived Brain Endothelial Cells

Leo M. Endres¹, Alexandra Schubert-Unkmeir¹, Brandon J. Kim^1,2

¹Institute for Hygiene and Microbiology, University of Würzburg, ²Department of Biological Sciences, University of Alabama

JoVE 61400

Methods Collection

Detection of microRNA Expression in the Kidneys of Immunoglobulin a Nephropathic Mice

Shohei Kaneko¹, Katsunori Yanai¹, Hiroki Ishii¹, Akinori Aomatsu^1,2, Kiyonori Ito¹, Keiji Hirai¹, Susumu Ookawara¹, Kenichi Ishibashi³, Yoshiyuki Morishita¹

¹Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, ²Division of Intensive Care Unit, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, ³Department of Medical Physiology, Meiji Pharmaceutical University

JoVE 61535

An Ex vivo Assay to Study Candida albicans Hyphal Morphogenesis in the Gastrointestinal Tract

Ross Monasky¹, Sonia Villa², Shankar Thangamani³

¹College of Veterinary Medicine, Midwestern University, ²Masters in Biomedical Science Program, College of Graduate Studies, Midwestern University, ³Department of Pathology and Population Medicine, College of Veterinary Medicine, Midwestern University

JoVE 61488

Field Postmortem Rabies Rapid Immunochromatographic Diagnostic Test for Resource-Limited Settings with Further Molecular Applications

Stephanie Mauti¹, Monique Léchenne², Service Naïssengar³, Abdallah Traoré⁴, Vessaly Kallo^5,6, Casimir Kouakou⁷, Emmanuel Couacy-Hymann⁷, Morgane Gourlaouen⁸, Céline Mbilo^9,10, Pati Patient Pyana¹¹, Enos Madaye³, Ibrahima Dicko⁴, Pascal Cozette¹, Paola De Benedictis⁸, Hervé Bourhy¹, Jakob Zinsstag^9,10, Laurent Dacheux¹

¹Unit Lyssavirus Epidemiology and Neuropathology, National Reference Center for Rabies and WHO Collaborating Center for Reference and Research on Rabies, Institut Pasteur, ²Environment and Sustainability Institute, University of Exeter, Penryn Campus, ³Institut de Recherche en Elevage pour le Développement, ⁴Laboratoire Central Vétérinaire, ⁵Direction des Services Vétérinaires, ⁶Ecole Inter Etats de Sciences et de Médecine Vétérinaires de Dakar, ⁷Laboratoire Central Vétérinaire de Bingerville, Laboratoire National d'Appui au Développement Agricole Bingerville, ⁸FAO Reference Centre for Rabies, Istituto Zooprofilattico Sperimentale delle Venezie, ⁹Swiss Tropical and Public Health Institute, ¹⁰University of Basel, ¹¹Institut National de Recherche Biomédicale

JoVE 60008

Isolation of Mouse Kidney-Resident CD8⁺ T cells for Flow Cytometry Analysis

Wei Liao^1,2, Chaoyu Ma¹, Nu Zhang¹

¹Department of Microbiology, Immunology and Molecular Genetics, School of Medicine, University of Texas Health Science Center at San Antonio, ²Department of Dermatology, Hunan Key Laboratory of Medical Epigenomics, The Second Xiangya Hospital, Central South University

JoVE 61559

Methods Collection

Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice

Meng Wang*¹, Yuanyuan Huang*¹, Wangpeng Gu^2,3, Haikun Wang¹

¹CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, University of Chinese Academy of Sciences, ²School of Life Science, University of Science and Technology of China, ³State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences

JoVE 60523

A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks

Toni Luise Meister*¹, Mara Klöhn*¹, Eike Steinmann¹, Daniel Todt¹

¹Molecular and Medical Virology, Ruhr University Bochum

JoVE 61373

Optimization of the Cuff Technique for Murine Heart Transplantation

Yunhan Ma*^1,2, Baiyi Xie*^1,2, Helong Dai*^3,4,5, Chenxi Wang⁶, Shujiao Liu⁶, Tianshu Lan⁷, Shuangyue Xu^1,2, Guoliang Yan^1,2,6, Zhongquan Qi^1,2,8

¹Organ Transplantation Institute, School of Medicine, Xiamen University, ²Fujian Key Laboratory of Organ and Tissue Regeneration, ³Department of Kidney Transplantation, Center of Organ Transplantation, The Second Xiangya Hospital of Central South University, ⁴Clinical Research Center for Organ Transplantation in Hunan Province, ⁵Clinical Immunology Center, Central South University, ⁶School of Medicine, Xiamen University, ⁷Xiamen Medical College, ⁸Medical College, Guangxi University

JoVE 61103

Characterization of Immune Cells and Proinflammatory Mediators in the Pulmonary Environment

Kelly L. Monaghan*¹, Breanne Y. Farris*¹, Wen Zheng¹, Edwin C. K. Wan^1,2,3

¹Department of Microbiology, Immunology, and Cell Biology, West Virginia University, ²Department of Neuroscience, West Virginia University, ³Rockefeller Neuroscience Institute, West Virginia University

JoVE 61359

Analysis of Human Natural Killer Cell Metabolism

Javier Traba^1,2, Thomas A. Waldmann³, Olga M. Anton³

¹Cardiovascular Branch, National Heart, Lung and Blood Institute, National Institutes of Health, ²Departamento de Biología Molecular, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid (CSIC-UAM), ³Lymphoid Malignancies Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health

JoVE 61466

Supervised Machine Learning for Semi-Quantification of Extracellular DNA in Glomerulonephritis

Kim Maree O'Sullivan¹, Sarah Creed², Poh-Yi Gan¹, Stephen R. Holdsworth^1,3

¹Department of Medicine, Monash University, ²Monash Micro Imaging, Monash University, ³Immunology Department, Monash Health

JoVE 61180

Production of Germ-Free Fast-Growing Broilers from a Commercial Line for Microbiota Studies

Edouard Guitton¹, Arnaud Faurie¹, Sebastien Lavillatte¹, Thierry Chaumeil¹, Pauline Gaboriaud², Françoise Bussière², Fabrice Laurent², Sonia Lacroix-Lamandé², Rodrigo Guabiraba², Catherine Schouler²

¹INRAE, PFIE, UE-1277, ²INRAE, ISP, Université de Tours

JoVE 61148