JoVE Journal

Immunology and Infection

JoVE Immunology and Infection includes methods and techniques for studying the immune system, mechanisms of infection, biological responses to pathogens, and therapeutic agents for treating immunological disease.

‹1 2 3 4 5 6 7 8 9…50 › 1,179 VIDEO ARTICLES

Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production

Aditi Upadhye¹, Melissa Marshall², James C. Garmey², Timothy P. Bender³, Coleen McNamara²

¹Department of Microbiology, Immunology, Cancer Biology, University of Virginia, ²Cardiovascular Research Center, University of Virginia, ³Beirne B. Carter Center for Immunology Research, University of Virginia

JoVE 61003

Identification and Characterization of Immunogenic RNA Species in HDM Allergens that Modulate Eosinophilic Lung Inflammation

Hamad H. Alanazi^1,2, Li She^1,3, Xiao-Dong Li¹

¹Department of Microbiology, Immunology and Molecular Genetics, Long School of Medicine, University of Texas Health San Antonio, ²Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, ³Department of Otolaryngology-Head and Neck Surgery, Xiangya Hospital, Central South University

JoVE 61183

Induction of Drug-Induced, Autoimmune Hepatitis in BALB/c Mice for the Study of Its Pathogenic Mechanisms

Dominic Thomas¹, Ting Yu Wu¹, Merylin Cottagiri¹, Maeva Nyandjo¹, Dolores B. Njoku^1,2

¹Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University, ²Department of Pediatrics and Pathology, Johns Hopkins University

JoVE 59174

In Vitro Microfluidic Disease Model to Study Whole Blood-Endothelial Interactions and Blood Clot Dynamics in Real-Time

Xue D. Manz¹, Hugo J. Albers^2,3, Petr Symersky⁴, Jurjan Aman¹, Andries D. van der Meer³, Harm Jan Bogaard¹, Robert Szulcek^1,5,6

¹Department of Pulmonary Diseases, Amsterdam UMC, VU University Medical Center, Amsterdam Cardiovascular Sciences (ACS), ²BIOS Lab-on-a-Chip group, University of Twente, ³Applied Stem Cell Technologies Group, University of Twente, ⁴Department of Cardio-thoracic Surgery, Amsterdam UMC, VU University Medical Center, ⁵Institute of Physiology, Charité-Universitätsmedizin, ⁶German Heart Center

JoVE 61068

Methods Collection

Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection

Sameera Vipat*¹, Greg Brennan*¹, Chorong Park¹, Sherry L. Haller², Stefan Rothenburg¹

¹Department of Medial Microbiology and Immunology, School of Medicine, University of California Davis, ²Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston

JoVE 61049

Assessment of Human Natural Killer Cell Events Driven by FcγRIIIa Engagement in the Presence of Therapeutic Antibodies

Annalise Petriello¹, Jacob A. Becerra², Jason Lay¹, Reem Husaini², Sarah L. Windler¹, Omar Duramad¹, Scot D. Liu¹

¹Department of Research and Development, iQ Biosciences, ²Department of Cellular Products, iQ Biosciences

JoVE 61144

Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO⁺ and Light Sheet Fluorescence Microscopy

Laurent Jacob*¹, Jose Brito*^1,2, Jean-Leon Thomas^1,3

¹Université Pierre et Marie Curie Paris, Sorbonne Université, Institut du Cerveau et de la Moelle Epinière, ²Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, ³Department of Neurology, Yale University School of Medicine

JoVE 61099

Assessing the Expression of Major Histocompatibility Complex Class I on Primary Murine Hippocampal Neurons by Flow Cytometry

Kristen E. Funk¹, Sarah K. Lotz¹

¹Department of Biological Sciences, University of North Carolina at Charlotte

JoVE 61436

Methods Collection

Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune cells

Krista D. DiSano¹, Michael R. Linzey^1,2, Nora C. Welsh^1,2, Joshua S. Meier¹, Andrew R. Pachner¹, Francesca Gilli¹

¹Department of Neurology, Geisel School of Medicine, Dartmouth-Hitchcock Medical Center, ²Program in Experimental and Molecular Medicine, Dartmouth College

JoVE 61166

Opsono-Adherence Assay to Evaluate Functional Antibodies in Vaccine Development Against Bacillus anthracis and Other Encapsulated Pathogens

Jennifer Chua*¹, Donald J. Chabot*¹, Tammy Putmon-Taylor^1,3, Arthur M. Friedlander²

¹Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, ²Headquarters Division, United States Army Medical Research Institute of Infectious Diseases, ³Bioqual

JoVE 60873

Measuring Erythrocyte Complement Receptor 1 Using Flow Cytometry

Aymric Kisserli^1,2, Sandra Audonnet³, Valérie Duret^2,4, Thierry Tabary^2,4, Jacques Henri Max Cohen², Rachid Mahmoudi^5,6

¹Oncogeriatric Coordination Unit, Reims University Hospitals, Maison Blanche Hospital, ²Faculty of Medicine, University of Reims Champagne-Ardenne, ³URCACyt, Flow cytometry technical platform, University of Reims Champagne-Ardenne, ⁴Department of Immunology, Reims University Hospitals, Robert Debre Hospital, ⁵Department of Internal Medicine and Geriatrics, Reims University Hospitals, Maison Blanche Hospital, ⁶Faculty of Medicine, University of Reims Champagne-Ardenne

JoVE 60810

Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Specific and Rapid Detection of Tilapia Lake Virus

Theerawut Phusantisampan¹, Pattarasuda Rawiwan^2,3, Sri Rajiv Kumar Roy², Malinee Sriariyanun⁴, Win Surachetpong^2,3

¹Department of Biotechnology, Faculty of Applied Science, King Mongkut's University of Technology North Bangkok (KMUTNB), ²Department of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, ³Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies, Kasetsart University, ⁴Department of Chemical and Process Engineering, The Sirindhorn Thai-German International Graduate School of Engineering (TGGS), King Mongkut's University of Technology North Bangkok (KMUTNB)

JoVE 61025

Generation of Human Regulatory T Cell Clones

Johannes Nowatzky¹, Olivier Manches^2,3

¹Department of Medicine, Division of Rheumatology, New York University School of Medicine, ²Immunobiology and Immunotherapy in Chronic Diseases, Institute for Advanced Biosciences, Université Grenoble Alpes, Inserm U1209/CNRS UMR 5309, ³Etablissement Français du Sang Auvergne-Rhône-Alpes

JoVE 61075

TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks

Yongliang Zhang*¹, Yuanyuan Li*^2,3, Xinxin Yang¹, Zhiyan Wen¹, Ugrappa Nagalakshmi², Savithramma P. Dinesh-Kumar^2,3

¹State Key Laboratory of Agro-Biotechnology and Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, ²Department of Plant Biology, College of Biological Sciences, University of California, Davis, ³Genome Center, College of Biological Sciences, University of California, Davis

JoVE 60728

Infection of Zebrafish Larvae with Aspergillus Spores for Analysis of Host-Pathogen Interactions

Savini Thrikawala¹, Emily E. Rosowski¹

¹Department of Biological Sciences, Clemson University

JoVE 61165

Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice

Yujia Lin^1,2, Jingna Xue², Shan Liao²

¹Department of General Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin Medical University, ²Inflammation Research Network, Department of Microbiology, Immunology and Infectious Diseases, Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary

JoVE 61178

Phage Therapy Application to Counteract Pseudomonas aeruginosa Infection in Cystic Fibrosis Zebrafish Embryos

Marco Cafora^1,2, Francesca Forti³, Federica Briani³, Daniela Ghisotti³, Anna Pistocchi²

¹Dipartimento di Scienze Cliniche e Comunità, Università degli Studi di Milano, ²Dipartimento di Biotecnologie Mediche e Medicina Traslazionale, Università degli Studi di Milano, LITA, ³Dipartimento di Bioscienze, Università degli Studi di Milano

JoVE 61275

Establishing a Porcine Ex Vivo Cornea Model for Studying Drug Treatments against Bacterial Keratitis

Katarzyna Okurowska^1,2, Sanhita Roy³, Praveen Thokala⁴, Lynda Partridge^1,5, Prashant Garg³, Sheila MacNeil^1,6, Peter N. Monk^1,7, Esther Karunakaran^1,2

¹Sheffield Collaboratorium for Antimicrobial Resistance and Biofilms (SCARAB), University of Sheffield, ²Department of Chemical and Biological Engineering, University of Sheffield, ³Hyderabad Eye Research Foundation, L V Prasad Eye Institute, ⁴Health Economics and Decision Science, School of Health and Related Research, University of Sheffield, ⁵Department of Molecular Biology and Biotechnology, University of Sheffield, ⁶Department of Materials Science and Engineering, University of Sheffield, ⁷Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield