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JoVE Journal

Immunology and Infection

JoVE Immunology and Infection includes methods and techniques for studying the immune system, mechanisms of infection, biological responses to pathogens, and therapeutic agents for treating immunological disease.

‹1…4 5 6 7 8 9…50 › 1,179 VIDEO ARTICLES

Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR

Fei-Xue Ban¹, Tian-Yan Yin¹, Qi Guo¹, Li-Long Pan¹, Yin-Quan Liu¹, Xiao-Wei Wang¹

¹Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogen and Insects, Institute of Insect Sciences, Zhejiang University

Humanized NOG Mice for Intravaginal HIV Exposure and Treatment of HIV Infection

Anna H. F. Andersen^1,2, Stine S. F. Nielsen^1,2, Rikke Olesen^1,2, Katharina Mack¹, Frederik Dagnæs-Hansen³, Niels Uldbjerg^1,4, Lars Østergaard^1,2, Ole S. Søgaard^1,2, Paul W. Denton^1,2,5, Martin Tolstrup^1,2

¹Department of Clinical Medicine, Aarhus University, ²Department of Infectious Diseases, Aarhus University Hospital, ³Department of Biomedicine, Aarhus University, ⁴Department of Gynecology and Obstetrics, Aarhus University Hospital, ⁵Department of Biology, University of Nebraska at Omaha

Adoptive Immunotherapy of iNKT Cells in Glucose-6-Phosphate Isomerase (G6PI)-Induced RA Mice

Ming Meng*^1,2, Shengde Chen*^1,2, Xiang Gao*^1,2, Huifang Liu*^1,2, Yuanyuan Wang*^1,2, Jingnan Zhang*^1,2, Haiyang Dou*^1,2, Wenjuan Li*^1,2, Dongzhi Chen*^1,2

¹Medical School of Hebei University, ²Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-Autoimmune Diseases in Hebei Province

Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis

Gabrielle Keb¹, Kenneth A. Fields¹

¹Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky

Monitoring Extracellular pH in Cross-Kingdom Biofilms using Confocal Microscopy

Sebastian Schlafer¹, Mathilde Frost Kristensen¹

¹Department of Dentistry and Oral Health, Aarhus University

In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction

Hongzheng Bi^1,2, Samiksha Wasnik¹, David J. Baylink¹, Chenfan Liu^1,3,4, Xiaolei Tang^1,3

¹Division of Regenerative Medicine, Department of Medicine, Loma Linda University, ²Zhengzhou University, ³Department of Veterinary Biomedical Sciences, College of Veterinary Medicine, Long Island University, ⁴Jinan Infectious Disease Hospital, Shandong University

Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils

Jeffrey W. Chadwick^1,2, Michael Glogauer^1,2

¹Department of Dental Oncology and Maxillofacial Prosthetics, Princess Margaret Cancer Centre, University Health Network, ²Faculty of Dentistry, University of Toronto

Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses

Fengwei Zhang¹, Yanan Wang², Xuechai Shang¹, Shanshan Wang¹, Rong Xiao¹, Hongjuan Zhou¹, Long Cai¹

¹Central Laboratory, Hang Zhou Red Cross Hospital, ²Clinical Laboratory, Yuhang District Maternity and Child Health Care Hospital

NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells

Jonathan M. Mendez¹, A. Marijke Keestra-Gounder¹

¹Department of Immunology and Microbiology, University of Colorado Anschutz Medical Campus

Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection

Meagan E. Valentine¹, Brandon D. Kirby¹, Hongwei D. Yu^1,2

¹Progenesis Technologies, LLC, ²Department of Biomedical Sciences, Pediatrics, Joan C. Edwards School of Medicine at Marshall University

Quantifying the Cytotoxicity of Staphyloccus aureus Against Human Polymorphonuclear Leukocytes

Jennifer G. Dankoff¹, Kyler B. Pallister¹, Fermin E. Guerra¹, Alexander J. Parks¹, Kelly Gorham², Saul Mastandrea², Jovanka M. Voyich¹, Tyler K. Nygaard¹

¹Department of Microbiology and Immunology, Montana State University, ²University Communications, Montana State University

Imaging the Human Immunological Synapse

Ana Bello-Gamboa*^1,2, Juan Manuel Izquierdo^1,2, Marta Velasco^1,2, Solange Moreno^1,2, Alejandro Garrido^1,2, Laura Meyers^1,2, Juan Carlos Palomino³, Víctor Calvo^1,2, Manuel Izquierdo*^1,2

¹Instituto de Investigaciones Biomédicas Alberto Sols, CSIC-Universidad Autónoma de Madrid, ²Departamento de Bioquímica, Instituto de Investigaciones Biomédicas Alberto Sols CSIC-UAM, Facultad de Medicina, Universidad Autónoma de Madrid, ³Unidad de Audiovisuales, Facultad de Medicina, Universidad Autónoma de Madrid

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

Xin Hu¹, Jaimee R. Compton², Patricia M. Legler²

¹National Center for Advancing Translational Sciences, National Institutes of Health, ²United States Naval Research Laboratory

Implantation and Monitoring by PET/CT of an Orthotopic Model of Human Pleural Mesothelioma in Athymic Mice

Didier J. Colin¹, Olivia Bejuy¹, Stéphane Germain¹, Frédéric Triponez², Véronique Serre-Beinier²

¹MicroPET/SPECT/CT Imaging Laboratory, Centre for BioMedical Imaging (CIBM), Division of Nuclear Medicine and Molecular Imaging, University Hospitals and University of Geneva, ²Division of Thoracic and Endocrine Surgery, University Hospitals and University of Geneva

Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens

Geetika Bajpai¹, Kory J. Lavine^1,2,3

¹Department of Medicine, Washington University School of Medicine, ²Department of Immunology and Pathology, Washington University School of Medicine, ³Department of Developmental Biology, Washington University School of Medicine

Isolation, Transfection, and Culture of Primary Human Monocytes

Karlie Plaisance-Bonstaff^1,2, Celeste Faia², Dorota Wyczechowska¹, Duane Jeansonne¹, Cecilia Vittori^1,3, Francesca Peruzzi^1,2

¹Department of Medicine, Louisiana State University Health Sciences Center, ²Department of Microbiology, Immunology, and Parasitology, Louisiana State University Health Sciences Center, ³Department of Biomedical and Clinical Sciences L. Sacco, University of Milan

Visualization and Quantification of High-Dimensional Cytometry Data using Cytofast and the Upstream Clustering Methods FlowSOM and Cytosplore

Guillaume Beyrend¹, Koen Stam², Ferry Ossendorp¹, Ramon Arens¹

¹Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, ²Department of Parasitology, Leiden University Medical Center

Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry

Susann Hartung^1,2, Christopher Rauh², Sarah Böttcher¹, Mai Thi Ngoc Hoang^1,2, Susanne Jahreis^1,2, Silke Rummler³, Andreas Hochhaus², Marie von Lilienfeld-Toal^1,2

¹Infections in Hematology and Oncology, Leibniz Institute for Infection Biology and Natural Product Research, ²Department for Hematology and Medical Oncology, Jena University Hospital, ³Institute for Transfusion Medicine, Jena University Hospital

Chronic, Acute, and Reactivated HIV Infection in Humanized Immunodeficient Mouse Models

Federico Perdomo-Celis^1,2, Sandra Medina-Moreno¹, Alonso Heredia¹, Harry Davis¹, Joseph Bryant¹, Juan Carlos Zapata¹

¹Institute of Human Virology, School of Medicine, University of Maryland, ²Grupo Inmunovirología, Facultad de Medicina, Universidad de Antioquia

Multi-scale Analysis of Bacterial Growth Under Stress Treatments

Julien Cayron¹, Christian Lesterlin¹

¹Molecular Microbiology and Structural Biochemistry (MMSB), University of Lyon 1, CNRS, Inserm

Automated Image-Based Quantification of Neutrophil Extracellular Traps Using NETQUANT

Tirthankar Mohanty¹, Pontus Nordenfelt¹

¹Department of Clinical Sciences, Division of Infection Medicine, Lund University

Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria

Robert S. Brzozowski¹, Maria L. White¹, Prahathees J. Eswara¹

¹Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida

A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells

Biao Xi¹, Robert Berahovich², Hua Zhou², Shirley Xu², Yuehua Wei², Jasper Guan², Hizkia Harto², Jian Guan², Lijun Wu², David Santa Ana^1,3, Fabio Cerignoil¹, Brandon Lamarche¹, Yama A. Abassi¹, Vita Golubovskaya²

¹ACEA Biosciences, Inc, ²ProMab Biotechnologies. Inc, ³ACEA Therapeutics

Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA

Marc Herb¹, Alina Farid¹, Alexander Gluschko¹, Martin Krönke^1,2,3,4, Michael Schramm¹

¹Institute for Medical Microbiology, Immunology and Hygiene, Faculty of Medicine and University Hospital Cologne, University of Cologne, ²Center for Molecular Medicine Cologne, ³Cologne Cluster of Excellence on Cellular Stress Responses in Aging-associated Diseases (CECAD), ⁴German Center for Infection Research (DZIF)

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