JoVE Journal

Medicine

JoVE Medicine includes methods and experimental approaches for biomedical research and clinical medicine, including case studies, clinical procedures, surgeries, clinical trials, and animal models of disease.

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Body Composition and Metabolic Caging Analysis in High Fat Fed Mice

Graeme I. Lancaster¹, Darren C. Henstridge¹

¹Baker Heart and Diabetes Institute

Impact of Intracardiac Neurons on Cardiac Electrophysiology and Arrhythmogenesis in an Ex Vivo Langendorff System

Christiane Jungen^1,2, Katharina Scherschel^1,2, Nadja I. Bork^2,3, Pawel Kuklik¹, Christian Eickholt¹, Helge Kniep¹, Niklas Klatt^1,2, Stephan Willems^1,2, Viacheslav O. Nikolaev^2,3, Christian Meyer^1,2

¹Department of Cardiology-Electrophysiology, cNEP (cardiac Neuro- and Electrophysiology research group), University Heart Center, University Hospital Hamburg-Eppendorf, ²DZHK (German Center for Cardiovascular Research), ³Institute of Experimental Cardiovascular Research, University Medical Center Hamburg-Eppendorf

Emergency Undocking in Robotic Surgery: A Simulation Curriculum

Derek Ballas^1,2, Megan Cesta¹, G. Dante Roulette¹, Margaret Rusnak³, Rami Ahmed²

¹Department of Obstetrics & Gynecology, Summa Health, ²Department of Medical Education, Summa Health, ³Ohio University College of Osteopathic Medicine

Surgical Labyrinthectomy of the Rat to Study the Vestibular System

Mun Young Chang¹, Moo Kyun Park², So Hyeon Park², Myung-Whan Suh², Jun Ho Lee², Seung Ha Oh²

¹Department of Otolaryngology-Head and Neck Surgery, Chung-Ang University College of Medicine, ²Department of Otolaryngology-Head and Neck Surgery, Seoul National University Hospital

Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia

Amy Stieler Stewart¹, John M Freund¹, Anthony T Blikslager¹, Liara M Gonzalez¹

¹Department of Clinical Sciences, North Carolina State University

Development and Functional Characterization of Murine Tolerogenic Dendritic Cells

Hsi-Ju Wei¹, John J. Letterio^2,3, Tej K. Pareek^2,3

¹Department of Biochemistry, School of Medicine, Case Western Reserve University, ²Department of Pediatrics, Division of Pediatric Hematology/Oncology, Case Western Reserve University, ³Angie Fowler Cancer Institute, Rainbow Babies & Children's Hospital, University Hospitals, Cleveland

Evaluation of Exon Inclusion Induced by Splice Switching Antisense Oligonucleotides in SMA Patient Fibroblasts

Rika Maruyama¹, Aleksander Touznik¹, Toshifumi Yokota^1,2

¹Department of Medical Genetics, University of Alberta Faculty of Medicine and Dentistry, ²Muscular Dystrophy Canada Research Chair, Department of Medical Genetics, University of Alberta Faculty of Medicine and Dentistry

Use of Two Intracorporeal Ventricular Assist Devices As a Total Artificial Heart

Muath Bishawi¹, Jun-Neng Roan^1,2, Jordan Richards¹, Zachary Brown¹, Laura Blue¹, Mani A. Daneshmand¹, Jacob N. Schroder¹, Dawn E. Bowles³, Carmelo A. Milano¹

¹Division of Cardiothoracic Surgery, Department of Surgery, Duke University, ²Division of Cardiovascular Surgery, Department of Surgery, National Cheng Kung University Hospital and College of Medicine, ³Division of Surgical Sciences, Department of Surgery, Duke University

Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion

Despoina Aslanoglou¹, Emily W. George¹, Zachary Freyberg^1,2

¹Department of Psychiatry, University of Pittsburgh, ²Department of Cell Biology, University of Pittsburgh

Photoselective Vaporesection of the Prostate via an End-firing Lithium Triborate Crystal Laser

Xin Wang¹, Yao-Guang Zhang¹, Sheng-Cai Zhu¹, Ben Wan¹, Ming Liu¹, Jian-Ye Wang¹

¹Department of Urology, Beijing Hospital, National Center of Gerontology

Treatment of Liver Metastases Using an Internal Target Volume Method for Stereotactic Body Radiotherapy

Wei-Jun Wang^1,2, Jeng-Fong Chiou^1,2,3, Yaoru Huang^1,2,4

¹Department of Radiation Oncology, Taipei Medical University Hospital, ²Department of Radiology, School of Medicine, College of Medicine, Taipei Medical University, ³Taipei Cancer Center, Taipei Medical University, ⁴Graduate Institute of Biomedical Materials & Tissue Engineering, College of Biomedical Engineering, Taipei Medical University

Preparation Steps for Measurement of Reactivity in Mouse Retinal Arterioles Ex Vivo

Adrian Gericke¹, Evgeny Goloborodko¹, Norbert Pfeiffer¹, Caroline Manicam¹

¹Department of Ophthalmology, University Medical Center of the Johannes Gutenberg University Mainz

Induction of Nephrotic Syndrome in Mice by Retrobulbar Injection of Doxorubicin and Prevention of Volume Retention by Sustained Release Aprotinin

Bernhard N. Bohnert^1,2,3, Ferruh Artunc^1,2,3

¹Department of Internal Medicine, Division of Endocrinology, Diabetology, Vascular Disease, Nephrology and Clinical Chemistry, University Hospital Tübingen, ²Institute of Diabetes Research and Metabolic Diseases (IDM) of the Helmholtz Center Munich, University Tübingen, ³German Center for Diabetes Research (DZD), University Tübingen

In Vivo Gene Transfer to the Rabbit Common Carotid Artery Endothelium

Bradley K. Wacker¹, Lianxiang Bi¹, David A. Dichek¹

¹Department of Medicine, University of Washington

Intraportal Transplantation of Pancreatic Islets in Mouse Model

Rahul Khatri¹, Birte Hussmann¹, Divya Rawat¹, Ali Osman Gürol², Thomas Linn¹

¹Third Medical Department, Clinical Research Lab, ²Istanbul University Department of Immunology, Institute of Experimental Medicine (DETAE)

A Drosophila In Vivo Injury Model for Studying Neuroregeneration in the Peripheral and Central Nervous System

Dan Li*¹, Feng Li*¹, Pavithran Guttipatti¹, Yuanquan Song^1,2

¹Raymond G. Perelman Center for Cellular and Molecular Therapeutics, The Children's Hospital of Philadelphia, ²Department of Pathology and Laboratory Medicine, University of Pennsylvania

Murine Salivary Functional Assessment via Pilocarpine Stimulation Following Fractionated Radiation

Jomy J. Varghese¹, Isaac L. Schmale², Mollie Eva Hansen¹, Shawn D. Newlands², Danielle S.W. Benoit¹, Catherine E. Ovitt³

¹Department of Biomedical Engineering, University of Rochester, ²Department of Otolaryngology, University of Rochester Medical Center, ³Center for Oral Biology, University of Rochester Medical Center

Measuring the Carotid to Femoral Pulse Wave Velocity (Cf-PWV) to Evaluate Arterial Stiffness

Hongwei Ji¹, Jing Xiong¹, Shikai Yu¹, Chen Chi¹, Bin Bai¹, Jiadela Teliewubai¹, Yuyan Lu¹, Yi Zhang¹, Yawei Xu¹

¹Department of Cardiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine

Muscle Imbalances: Testing and Training Functional Eccentric Hamstring Strength in Athletic Populations

Petr Stastny¹, Michal Lehnert², James J. Tufano¹

¹Faculty of Physical Education and Sport, Charles University, ²Faculty of Physical Culture, Palacky University Olomouc

Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae

Tali Farine^1,2, Michael Parsons², Stephen Lye^1,2,3, Oksana Shynlova^1,2,3

¹Department of Physiology, University of Toronto, ²Lunenfeld-Tanenbaum Research Institute, Sinai Health System, ³Department of Obstetrics and Gynecology, University of Toronto

Cavernous Nerve Stimulation and Recording of Intracavernous Pressure in a Rat

Morten Hox¹, Travis Mann-Gow², Lars Lund^3,4, Peter Zvara^1,4

¹Biomedical Laboratory and the Research Unit of Urology, Department of Clinical Research, University of Southern Denmark, ²Department of Surgery, University of Vermont, ³Research Unit of Urology, Department of Clinical Research, University of Southern Denmark, ⁴Department of Urology, Odense University Hospital

An Experimental Model of Diet-Induced Metabolic Syndrome in Rabbit: Methodological Considerations, Development, and Assessment

Óscar Julián Arias-Mutis^1,2,3, Patricia Genovés^1,2,3, Conrado J. Calvo^1,2,4, Ana Díaz⁵, Germán Parra^2,3, Luis Such-Miquel⁶, Luis Such², Antonio Alberola², Francisco Javier Chorro^1,3, Manuel Zarzoso⁶

¹CIBERCV, Instituto de Salud Carlos III, ²Department of Physiology, Universitat de València, ³INCLIVA, ⁴Department of Electronic Engineering, Universidad Politécnica de Valencia, ⁵UCIM, Universitat de València, ⁶Department of Physiotherapy, Universitat de València

Spatial Quantification of Drugs in Pulmonary Tuberculosis Lesions by Laser Capture Microdissection Liquid Chromatography Mass Spectrometry (LCM-LC/MS)

Matthew Zimmerman¹, Landry Blanc¹, Pei-Yu Chen¹, Véronique Dartois¹, Brendan Prideaux¹

¹Public Health Research Institute, New Jersey Medical School, Rutgers, The State University of New Jersey

Generation of Human Nasal Epithelial Cell Spheroids for Individualized Cystic Fibrosis Transmembrane Conductance Regulator Study

John J. Brewington¹, Erin T. Filbrandt¹, Francis J. LaRosa III¹, Jessica D. Moncivaiz¹, Alicia J. Ostmann¹, Lauren M. Strecker¹, John P. Clancy¹

¹Department of Pediatrics, Division of Pulmonary Medicine, Cincinnati Children's Hospital Medical Center

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