JoVE Journal

Medicine

JoVE Medicine includes methods and experimental approaches for biomedical research and clinical medicine, including case studies, clinical procedures, surgeries, clinical trials, and animal models of disease.

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A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids

Jorik M. van Rijn^1,2,3, Marliek van Hoesel^1,2, Sabine Middendorp^1,2

¹Division of Pediatrics, Department of Pediatric Gastroenterology, Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht University, ²Regenerative Medicine Center, University Medical Center Utrecht, Utrecht University, ³Science for Life Laboratory, Department of Medical Biochemistry and Microbiology, Uppsala University

Optimized Griess Reaction for UV-Vis and Naked-eye Determination of Anti-malarial Primaquine

Yalan Wu*¹, Shengjun Wu*¹, Xin-an Huang¹, Qingping Zeng¹, Tao Deng¹, Fang Liu¹

¹Institute of Tropical Medicine, Guangzhou University of Chinese Medicine

Use of Magnetic Resonance Imaging and Biopsy Data to Guide Sampling Procedures for Prostate Cancer Biobanking

Susan Heavey¹, Aiman Haider², Ashwin Sridhar³, Hayley Pye¹, Greg Shaw¹, Alex Freeman², Hayley Whitaker¹

¹Molecular Diagnostics and Therapeutics Group, University College London, ²Research Department of Pathology, University College London, ³Department of Uro-Oncology, UCLH NHS Foundation Trust

A Porcine Corneal Endothelial Organ Culture Model Using Split Corneal Buttons

Daniel A. Wenzel¹, Berenike C. Kunzmann², Nils A. Steinhorst¹, Martin S. Spitzer¹, Maximilian Schultheiss¹

¹Department of Ophthalmology, University Medical Center Hamburg-Eppendorf (UKE), ²University Eye Hospital, Center For Ophthalmology, University Hospital Tübingen

Generation of Porcine Testicular Organoids with Testis Specific Architecture using Microwell Culture

Sadman Sakib¹, Yang Yu^3,4, Anna Voigt², Mark Ungrin^2,3,4, Ina Dobrinski^1,2

¹Biochemistry and Molecular Biology, University of Calgary, ²Comparative Biology and Experimental Medicine, University of Calgary, ³Biomedical Engineering Graduate Program, University of Calgary, ⁴Alberta Diabetes Institute, University of Alberta

Precision Implementation of Minimal Erythema Dose (MED) Testing to Assess Individual Variation in Human Inflammatory Response

John A. Richey¹, Holly Sullivan-Toole², Marlene Strege¹, Corinne Carlton¹, Dylan McDaniel³, Matthew Komelski⁴, Amy Epperley⁵, Hongxiao Zhu⁶, Irving C. Allen^3,7

¹Department of Psychology, Virginia Tech, ²Graduate Program in Translational Biology, Medicine and Health, Virginia Tech, ³Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Tech, ⁴Department of Human Development and Family Science, Virginia Tech, ⁵Wellness Center, Virginia Tech, ⁶Department of Statistics, Virginia Tech, ⁷Department of Basic Science Education, Virginia Tech Carilion School of Medicine

Implantation of an Isoproterenol Mini-Pump to Induce Heart Failure in Mice

Shuxun Ren¹, Sunny Chang², Alex Tran³, Arianna Mandelli⁴, Yibin Wang¹, Jessica J. Wang²

¹Department of Anesthesiology, University of California, ²Department of Medicine, University of California, ³Department of Microbiology, Immunology & Molecular Genetics, University of California, ⁴Department of Molecular, Cell, and Developmental Biology, University of California

Integration of Brain Tissue Saturation Monitoring in Cardiopulmonary Exercise Testing in Patients with Heart Failure

Shu-Chun Huang^1,2,3, Carl PC Chen^1,3, Tieh-Cheng Fu⁴, Yu-Jen Chen^1,5,6

¹Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital, Linkou, ²Healthy Aging Research Center, Chang Gung University, ³School of Medicine, College of Medicine, Chang Gung University, ⁴Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital, Keelung, ⁵National Quemoy University, ⁶Kinmen Hospital, Ministry of Health and Welfare

Technical Detail for Robot Assisted Pancreaticoduodenectomy

Alex C. Kim¹, Rebecca C. Rist¹, Amer H. Zureikat¹

¹Department of Surgery, University of Pittsburgh Medical Center

Modified Single-Loop Reconstruction for Pancreaticoduodenectomy

Andreas M. Luu¹, Ilgar Aghalarov¹, Chris Braumann¹, Waldemar Uhl¹, Orlin Belyaev¹

¹Department of General and Visceral Surgery, St. Josef Hospital, Ruhr University Bochum

Scratch Migration Assay and Dorsal Skinfold Chamber for In Vitro and In Vivo Analysis of Wound Healing

Anouar Belkacemi¹, Matthias W. Laschke², Michael D. Menger², Veit Flockerzi¹

¹Institute of Experimental and Clinical Pharmacology and Toxicology, Center for Molecular Signaling (PZMS), Saarland University, ²Institute for Clinical and Experimental Surgery, Saarland University

Lung Fixation under Constant Pressure for Evaluation of Emphysema in Mice

Keiko Karasutani^1,2, Hario Baskoro¹, Tadashi Sato¹, Naoko Arano¹, Yohei Suzuki¹, Aki Mitsui¹, Naoko Shimada¹, Yuzo Kodama¹, Kuniaki Seyama¹, Yoshinosuke Fukuchi¹, Kazuhisa Takahashi¹

¹Department of Respiratory Medicine, Juntendo University Graduate School of Medicine, Juntendo University, ²Pharmaceutical Planning Group, Otsuka Pharmaceutical Co., Ltd.

Nanoscopic Imaging of Human Tissue Sections via Physical and Isotropic Expansion

Aleksandra Klimas¹, Octavian Bucur², Brigdet Njeri¹, Yongxin Zhao¹

¹Department of Biological Sciences, Carnegie Mellon University, ²QPathology

Protocol to Create Chronic Wounds in Diabetic Mice

Jane Hannah Kim¹, Manuela Martins-Green¹

¹Department of Molecular, Cell and Systems Biology, University of California, Riverside

A Clinical Trial Assessing the Safety, Efficacy, and Delivery of Olive-Oil-Based Three-Chamber Bags for Parenteral Nutrition

Zhenyi Jia¹, Jun Yang¹, Huanlong Qin^1,2

¹Department of General Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, ²Department of General Surgery, Shanghai Tenth People's Hospital Affiliated to Tongji University

A Cryoinjury Model to Study Myocardial Infarction in the Mouse

Dong Wang*^1,2, Grigol Tediashvili*^1,2, Xiaomeng Hu^1,2, Alessia Gravina², Sivan G. Marcus^1,2, Hao Zhang⁴, Jeffrey E Olgin⁴, Tobias Deuse^1,2,5, Sonja Schrepfer^1,2,3,5

¹Transplant and Stem Cell Immunobiology Lab, University Heart Center, ²Department of Surgery, Transplant and Stem Cell Immunobiology Lab, University of California San Francisco, ³Cardiovascular Research Center (CVRC) and DZHK German Center for Cardiovascular Research, ⁴Division of Cardiology, Cardiovascular Research Institute, University of California San Francisco, ⁵Cardiovascular Surgery, University Heart Center

Precision Measurements and Parametric Models of Vertebral Endplates

Hang Feng¹, Zhu Ziqi¹, Yu Bin¹, Xiaoming Liu¹, Shenbei Duo¹, Surendra Kumar Chaudhary¹, Wu Tongde¹, Xinhua Li¹, Zhaoyu Ba¹, Desheng Wu¹

¹Department of Spinal Surgery, Shanghai East Hospital, Tongji University School of Medicine

Immunofluorescence Labelling of Human and Murine Neutrophil Extracellular Traps in Paraffin-Embedded Tissue

Ulrike Abu Abed^1,2, Volker Brinkmann¹

¹Microscopy Core Facility, Max Planck Institute for Infection Biology, ²Cellular Microbiology, Max Planck Institute for Infection Biology

Rapid Evaluation of Toxicity of Chemical Compounds Using Zebrafish Embryos

Ashok Aspatwar¹, Milka Marjut Hammaren¹, Mataleena Parikka^1,2, Seppo Parkkila^1,3

¹Faculty of Medicine and Health Technology, Tampere University, ²Oral and Maxillofacial Unit, Tampere University Hospital, ³Fimlab Ltd, Tampere University Hospital

Murine Surgical Model of Topical Elastase Induced Descending Thoracic Aortic Aneurysm

Zachary Tyerman¹, Jolian Dahl¹, Alexander Shannon¹, W. Forrest Johnston², Nicholas H. Pope¹, Guanyi Lu³, Gilbert R. Upchurch Jr.³, Gorav Ailawadi^1,4, Morgan Salmon¹

¹Department of Surgery, University of Virginia School of Medicine, ²Department of Surgery, Ochsner Medical Center, ³Department of Surgery, University of Florida, ⁴The Robert M. Berne Cardiovascular Research Center, University of Virginia School of Medicine

Murine Appendectomy Model of Chronic Colitis Associated Colorectal Cancer by Precise Localization of Caecal Patch

Yaguang Li¹, Junhong Liu^2,3, Gaixia Liu¹, Zhenhong Pan^2,3, Mingxia Zhang¹, Yao Ma⁴, Qingxia Wei^1,5, Hongping Xia^1,6, Rui Xue Zhang^1,2, Junjun She¹

¹Department of General Surgery, The First Affiliated Hospital, Xi'an Jiaotong University, ²Institute of Medical Research, Northwestern Polytechnical University, ³School of Life Sciences, Northwestern Polytechnical University, ⁴Department of Medicine, Xi'an Jiaotong University, ⁵Princess Margaret Cancer Center, University of Health Network, ⁶Department of Pathology, Nanjing Medical University

Murine Myocardial Infarction Model using Permanent Ligation of Left Anterior Descending Coronary Artery

Jérôme Lugrin^1,2, Roumen Parapanov^1,2, Thorsten Krueger², Lucas Liaudet¹

¹Service of Adult Intensive Care Medicine, Department of Interdisciplinary Centers and Logistics, Lausanne University Hospital and Faculty of Biology and Medicine, Lausanne University, ²Service of Thoracic Surgery, Department of Surgery and Anesthesiology Services, Lausanne University Hospital and Faculty of Biology and Medicine, Lausanne University

Isolation and Purification of Murine Cardiac Pericytes

Linda L. Lee¹, Aarif Y. Khakoo², Vishnu Chintalgattu¹

¹Department of Cardiometabolic Disorders, Amgen Research and Discovery, Amgen Inc., ²Department of Translational Sciences, Amgen Research and Discovery, Amgen Inc.

Structured Motor Rehabilitation After Selective Nerve Transfers

Agnes Sturma^1,2,3, Laura A. Hruby^1,4, Dario Farina², Oskar C. Aszmann^1,5

¹Clinical Laboratory for Bionic Extremity Reconstruction, Medical University of Vienna, ²Bioengineering Department, Imperial College London, ³Master's Degree Program Health Assisting Engineering, University of Applied Sciences FH Campus Wien, ⁴Department of Orthopedics and Trauma Surgery, Medical University of Vienna, ⁵Division of Plastic and Reconstructive Surgery, Department of Surgery, Medical University of Vienna

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