JoVE Journal

Medicine

JoVE Medicine includes methods and experimental approaches for biomedical research and clinical medicine, including case studies, clinical procedures, surgeries, clinical trials, and animal models of disease.

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Intravital Microscopy of Tumor-associated Vasculature Using Advanced Dorsal Skinfold Window Chambers on Transgenic Fluorescent Mice

Ann L.B. Seynhaeve¹, Timo L.M. ten Hagen¹

¹Laboratory Experimental Surgical Oncology, Section Surgical Oncology, Department of Surgery, Erasmus MC

A Quantitative Sensory Testing Paradigm to Obtain Measures of Pain Processing in Patients Undergoing Breast Cancer Surgery

Noud van Helmond¹, Hans Timmerman¹, Søren S. Olesen², Asbjørn M. Drewes², Joris Kleinhans³, Oliver H. Wilder-Smith¹, Kris C. Vissers¹, Monique A. Steegers¹

¹Department of Anesthesiology, Pain, and Palliative Medicine, Radboud University Medical Center, ²Mech-Sense, Department of Gastroenterology & Hepatology, Aalborg University Hospital, ³Medical Technology & Clinical Physics, Radboud University Medical Center

Dissection and Explant Culture of Murine Allantois for the In Vitro Analysis of Allantoic Attachment

Kerstin Hadamek^1,2, Angelika Keller^1,2, Antje Gohla^1,2

¹Institute of Pharmacology and Toxicology, University of Würzburg, ²Rudolf Virchow Center for Experimental Biomedicine, University of Würzburg

Discovering Middle Ear Anatomy by Transcanal Endoscopic Ear Surgery: A Dissection Manual

Lukas Anschuetz^1,2, Livio Presutti², Daniele Marchioni³, Marco Bonali², Wilhelm Wimmer^1,4, Domenico Villari², Marco Caversaccio^1,4

¹Department of Otorhinolaryngology, Head and Neck Surgery, Inselspital, Bern University Hospital, University of Bern, ²Department of Otorhinolaryngology, Head & Neck Surgery, University Hospital of Modena, ³Department of Otorhinolaryngology, Head & Neck Surgery, University Hospital of Verona, ⁴Artificial Hearing Research, Artorg Center for Biomedical Engineering, University of Bern

Study of In Vivo Glucose Metabolism in High-fat Diet-fed Mice Using Oral Glucose Tolerance Test (OGTT) and Insulin Tolerance Test (ITT)

Csörsz Nagy¹, Elisa Einwallner¹

¹Department of Laboratory Medicine, Medical University of Vienna

Second Harmonic Generation Signals in Rabbit Sclera As a Tool for Evaluation of Therapeutic Tissue Cross-linking (TXL) for Myopia

Mariya Zyablitskaya¹, E. Laura Munteanu², Takayuki Nagasaki¹, David C. Paik¹

¹Department of Ophthalmology, Columbia University College of Physicians and Surgeons, ²Confocal and Specialized Microscopy Shared Resource, Herbert Irving Comprehensive Cancer Center, Columbia University

Studying the Hypothalamic Insulin Signal to Peripheral Glucose Intolerance with a Continuous Drug Infusion System into the Mouse Brain

Reni Ajoy^1,2, Szu-Yi Chou^1,2,3

¹The Ph.D. Program for Neural Regenerative Medicine, College of Medical Science and Technology, Taipei Medical University and National Health Research, ²Graduate Institute of Neural Regenerative Medicine, College of Medical Science and Technology, Taipei Medical University, ³TMU research center for Neurotrauma and Neuroregeneration, College of Medical Science and Technology, Taipei Medical University

Microscopy Based Methods for the Assessment of Epithelial Cell Migration During In Vitro Wound Healing

Sergio Liarte*¹, Ángel Bernabé-García*¹, David Armero-Barranco², Francisco José Nicolás¹

¹Laboratorio de Oncología Molecular y TGF-β, IMIB-Arrixaca, ²Departamento de Enfermería, Facultad Enfermería, Universidad de Murcia

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

Elisabeth Santigli¹, Martin Koller², Barbara Klug¹

¹Division of Oral Surgery and Orthodontics, Department of Dental Medicine and Oral Health, Medical University of Graz, ²Division of Preventive and Operative Dentistry, Periodontology, Prosthodontics and Restorative Dentistry, Department of Dental Medicine and Oral Health, Medical University of Graz

Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance

Chayanika Biswas*¹, Sharon C-A. Chen*^1,2,3, Catriona Halliday^1,2, Elena Martinez¹, Rebecca J. Rockett¹, Qinning Wang¹, Verlaine J. Timms¹, Rajat Dhakal¹, Rosemarie Sadsad¹, Karina J. Kennedy⁴, Geoffrey Playford^4,5, Deborah J. Marriott⁶, Monica A. Slavin⁷, Tania C. Sorrell^1,3, Vitali Sintchenko^1,2,3

¹Centre for Infectious Diseases and Microbiology-Public Health, Westmead Hospital, ²Centre for Infectious Diseases and Microbiology Laboratory Services, ICPMR, ³Marie Bashir Institute for Infectious Diseases and Biosecurity, The University of Sydney, ⁴Department of Microbiology and Infectious Diseases, Canberra Hospital and Health Services, Australian National University Medical School, ⁵Infection Management Services, Australian National University Medical School, ⁶Department of Microbiology and Infectious Diseases, St. Vincent's Hospital, ⁷Department of Infectious Diseases, Peter MacCallum Cancer Centre

Studying Diabetes Through the Eyes of a Fish: Microdissection, Visualization, and Analysis of the Adult tg(fli:EGFP) Zebrafish Retinal Vasculature

Lucas Moritz Wiggenhauser¹, Katharina Kohl², Nadine Dietrich², Hans-Peter Hammes², Jens Kroll¹

¹Department of Vascular Biology and Tumorangiogenesis, Center for Biomedicine and Medical Technology Mannheim (CBTM), Medical Faculty Mannheim, Heidelberg University, ²V. Medical Clinic, Medical Faculty Mannheim, Heidelberg University

In Vitro Enzyme Measurement to Test Pharmacological Chaperone Responsiveness in Fabry and Pompe Disease

Jan Lukas¹, Anne-Marie Knospe¹, Susanne Seemann¹, Valentina Citro², Maria V. Cubellis², Arndt Rolfs^1,3

¹Albrecht-Kossel-Institute, University Rostock Medical Center, ²Department of Biology, University Federico II, ³Centogene AG

Methodology for Sputum Induction and Laboratory Processing

Julien Guiot*¹, Sophie Demarche*^1,2, Monique Henket¹, Virginie Paulus¹, Sophie Graff¹, Florence Schleich¹, Jean-Louis Corhay¹, Renaud Louis¹, Catherine Moermans¹

¹Department of Respiratory Medicine, CHU Liege, GIGA I3 Research Group, University of Liege, ²Department of Clinical Pharmacy, CIRM (Center for Interdisciplinary Research on Medicines), University of Liege

Mouse Model for Pancreas Transplantation Using a Modified Cuff Technique

Benno Cardini*¹, Rupert Oberhuber*¹, Sven R Hein¹, Rebecca Eiter¹, Martin Hermann², Markus Kofler^1,3, Stefan Schneeberger¹, Gerald Brandacher^1,4, Manuel Maglione¹

¹Center of Operative Medicine, Department of Visceral, Transplant and Thoracic Surgery, Medical University Innsbruck, ²Department of Anesthesiology and Critical Care Medicine, Medical University Innsbruck, ⁴Department of Cardiac Surgery, Medical University Innsbruck, ³Department of Plastic and Reconstructive Surgery, Vascularized Composite Allotransplantation (VCA) Laboratory, Johns Hopkins University School of Medicine

Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation

Tobias M. Nowacki¹, Dominik Bettenworth¹, Markus Brückner¹, Friederike Cordes¹, Frank Lenze¹, Anne Becker², Moritz Wildgruber², Michel Eisenblätter²

¹Department of Medicine B, University Hospital Münster, ²Translational Research Imaging Center, Department of Clinical Radiology, University Hospital Münster

MRI-guided Focused Ultrasound Thalamotomy for Patients with Medically-refractory Essential Tremor

Ying Meng¹, Yuexi Huang², Benjamin Solomon², Kullervo Hynynen², Nadia Scantlebury¹, Michael L. Schwartz¹, Nir Lipsman¹

¹Division of Neurosurgery, Sunnybrook Health Sciences Centre, ²Sunnybrook Research Institute, Sunnybrook Health Sciences Centre

Standardized Technique of Aortic Valve Re-implantation for Valve-sparing Aortic Root Replacement

Reza Tavakoli^1,2, Guillaume Lebreton¹, Max Gassmann², Peiman Jamshidi³, Pascal Leprince¹

¹Department of Cardiovascular and Thoracic Surgery, Pitie Salpetriere University Hospital, Assistance Publique, Hopitaux de Paris (APHP), Institut de Cardiologie, ²Institute of Veterinary Physiology and Zurich Center for Integrative Human Physiology, University of Zurich, ³Herzzentrum Hirslanden, Klinik St Anna

Repeated Blood Collection from Tail Vein of Non-Anesthetized Rats with a Vacuum Blood Collection System

Wusong Zou¹, Yanqun Yang¹, Ye Gu¹, Pengfei Zhu¹, Mingjing Zhang¹, Zhong Cheng¹, Xiaoyan Liu¹, Yijun Yu¹, Xiaohong Peng¹

¹Heart Center at Puai Hospital, Wuhan Puai Hospital, Jianghan University

Evaluation of Vascular Control Mechanisms Utilizing Video Microscopy of Isolated Resistance Arteries of Rats

Kathleen M. Lukaszewicz¹, Matthew J. Durand², Jessica R.C. Priestley³, James R. Schmidt⁴, L. Adrienne Allen⁵, Aron M. Geurts³, Julian H. Lombard³

¹Department of Physical Therapy, Marquette University, ²Medical College of Wisconsin, ³Department of Physiology, Medical College of Wisconsin, ⁴Graduate Programs of Nurse Anesthesia, Texas Wesleyan University, ⁵Office of Research, Medical College of Wisconsin

Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction

Gunnar T.R. Hischebeth¹, Sascha Gravius², Johanna K. Buhr², Ernst Molitor¹, Matthias D. Wimmer², Achim Hoerauf¹, Isabelle Bekeredjian-Ding³, Thomas M. Randau²

¹Institute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, ²Department of Orthopaedics and Trauma Surgery, University Hospital Bonn, ³Division of EU Cooperation/Microbiology, Paul-Ehrlich-Institute

Ultrasonography in Experimental Reproductive Investigations on Rats

Tianjuang Wang^1,2, Lidia Oltra-Rodríguez³, Nuria García-Carrillo³, Anibal Nieto^4,5, Yunxia Cao*^1,2,6, María L. Sánchez-Ferrer*^4,5

¹Reproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, ²Institute of Reproductive Genetics, Anhui Medical University, ³Centro Experimental en Investigaciones Biomédica (CEIB), Campus Ciencias de la Salud, Universidad de Murcia, ⁴Department of Obstetrics and Gynecology, 'Virgen de la Arrixaca' University Clinical Hospital, ⁵Institute for Biomedical Research of Murcia, IMIB-Arrixaca, ⁶Anhui Provincial Engineering Technology Research Center for Biopreservation and Artificial Organs

An Optimized Hemagglutination Inhibition (HI) Assay to Quantify Influenza-specific Antibody Titers

Lukas Kaufmann*¹, Mohammedyaseen Syedbasha*¹, Dominik Vogt¹, Yvonne Hollenstein¹, Julia Hartmann¹, Janina E. Linnik^1,2,3, Adrian Egli^1,4

¹Applied Microbiology Research, Department of Biomedicine, University of Basel, ²Department of Biosystems Science and Engineering, ETH Zurich, ³Swiss Institute of Bioinformatics, ⁴Clinical Microbiology, University Hospital Basel

Electrophysiological Measurement of Noxious-evoked Brain Activity in Neonates Using a Flat-tip Probe Coupled to Electroencephalography

Noemi Klarer*¹, Hanna Rickenbacher*¹, Severin Kasser¹, Antoinette Depoorter², Sven Wellmann¹

¹Division of Neonatology, University of Basel Children's Hospital, ²Divison of Neuropediatrics and Developmental Medicine, University of Basel Children's Hospital

3D Ultrasound Imaging: Fast and Cost-effective Morphometry of Musculoskeletal Tissue

Guido Weide^1,2, Stephan van der Zwaard¹, Peter A. Huijing¹, Richard T. Jaspers*¹, Jaap Harlaar*²

¹Laboratory for Myology, Department of Human Movement Sciences, Faculty of Behavioural and Movement Sciences, Vrije Universiteit Amsterdam, Amsterdam Movement Sciences, ²Department of Rehabilitation Medicine, VU University Medical Center Amsterdam, Amsterdam Movement Sciences

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