JoVE Journal

Medicine

JoVE Medicine includes methods and experimental approaches for biomedical research and clinical medicine, including case studies, clinical procedures, surgeries, clinical trials, and animal models of disease.

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Complete and Partial Aortic Occlusion for the Treatment of Hemorrhagic Shock in Swine

Aaron M. Williams*¹, Umar F. Bhatti*¹, Isabel S. Dennahy¹, Kiril Chtraklin¹, Panpan Chang¹, Nathan J. Graham¹, Basil M. Baccouche¹, Shalini Roy¹, Mohammed Harajli¹, Jing Zhou¹, Vahagn C. Nikolian¹, Qiufang Deng¹, Yuzi Tian¹, Baoling Liu¹, Yongqing Li¹, Gregory L. Hays^1,2, Julia L. Hays^1,2, Hasan B. Alam¹

¹Department of Surgery, University of Michigan, ²Hays Innovations

Designing CAD/CAM Surgical Guides for Maxillary Reconstruction Using an In-house Approach

Toshiaki Numajiri¹, Daiki Morita², Hiroko Nakamura³, Ryo Yamochi¹, Shoko Tsujiko⁴, Yoshihiro Sowa¹

¹Department of Plastic and Reconstructive Surgery, Kyoto Prefectural University of Medicine, ²Department of Plastic and Reconstructive Surgery, Showa University Fujigaoka Hospital, ³Department of Plastic and Reconstructive Surgery, Fukuchiyama City Hospital, ⁴Department of Plastic and Reconstructive Surgery, Saiseikai Shiga Hospital

Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography

Jie Sun¹, Guang Ming Yang², Tao Tao¹, Li Sha Wei¹, Yang Pan², Min Sheng Zhu¹

¹Model Animal Research Center and MOE Key Laboratory of Model Animal and Disease Study, Nanjing University, ²School of Pharmacy, Nanjing University of Chinese Medicine

An In Vitro Approach to Photodynamic Therapy

Evan Austin^1,2, Jared Jagdeo^1,2,3

¹Department of Dermatology, University of California, Davis, ²Dermatology Service, Sacramento VA Medical Center, ³Department of Dermatology, State University of New York, Downstate Medical Center

Taste Exam: A Brief and Validated Test

Jennifer E. Douglas^1,2, Corrine J. Mansfield², Charles J. Arayata², Beverly J. Cowart², Lauren R. Colquitt², Ivy W. Maina^1,2, Mariel T. Blasetti³, Noam A. Cohen³, Danielle R. Reed²

¹Perelman School of Medicine at the University of Pennsylvania, ²Monell Chemical Senses Center, ³Department of Otorhinolaryngology-Head and Neck Surgery, Division of Rhinology, Hospital of the University of Pennsylvania

The Generation of Closed Femoral Fractures in Mice: A Model to Study Bone Healing

Justin N. Williams¹, Yong Li¹, Anuradha Valiya Kambrath¹, Uma Sankar¹

¹Department of Anatomy and Cell Biology, Indiana University School of Medicine

The Perinatal Asphyxiated Lamb Model: A Model for Newborn Resuscitation

Payam Vali*¹, Sylvia Gugino*², Carmon Koenigsknecht², Justin Helman², Praveen Chandrasekharan², Munmun Rawat², Satyan Lakshminrusimha¹, Jayasree Nair²

¹Department of Pediatrics, University of California Davis, ²Department of Pediatrics, University at Buffalo

Fracture Apparatus Design and Protocol Optimization for Closed-stabilized Fractures in Rodents

Robert L Zondervan^1,2, Mitch Vorce³, Nick Servadio⁴, Kurt D. Hankenson²

¹College of Osteopathic Medicine, Michigan State University, ²Department of Orthopaedic Surgery, University of Michigan Medical School, ³Lymann Briggs College, Michigan State University, ⁴College of Engineering, Michigan State University

Transplantation of Adipose Tissue-Derived Stem Cell Sheet to Reduce Leakage After Partial Colectomy in A Rat Model

Panithi Sukho^1,2,3, Geesien S.A. Boersema⁴, Nicole Kops⁵, Johan F. Lange⁴, Jolle Kirpensteijn^1,6, Jan Willem Hesselink¹, Yvonne M. Bastiaansen-Jenniskens⁵, Femke Verseijden^1,5

¹Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, ²Department of Otorhinolaryngology, Erasmus MC University Medical Center, ³Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, ⁴Department of Surgery, Erasmus MC University Medical Center, ⁵Department of Orthopaedics, Erasmus MC University Medical Center, ⁶Hill's Pet Nutrition Inc.

In Vivo Photolabeling of Cells in the Colon to Assess Migratory Potential of Hematopoietic Cells in Neonatal Mice

Caryn Porter¹, Maria Ennamorati², Nitya Jain²

¹Center for Computational and Integrative Biology, Massachusetts General Hospital, ²Mucosal Immunology and Biology Research Center, Massachusetts General Hospital

A Mobile Outside-in Technique of Transforaminal Lumbar Endoscopy for Lumbar Disc Herniations

Hyeun Sung Kim¹, Nitin Adsul¹, Ankur Kapoor¹, Sung Ho Choi², Jeong Hoon Kim², Ki Joon Kim², Jeong Soo Bang¹, Kyun Hoong Yang¹, Seok Han¹, Jae Hyun Lim¹, Jee-Soo Jang², II-Tae Jang¹, Seong-Hoon Oh³

¹Department of Neurosurgery, Nanoori Hospital, ²Department of Neurosurgery, Nanoori Suwon Hospital, ³Department of Neurosurgery, Nanoori Incheon Hospital

Assessment of Plasma Coagulation on Liver Tissue in a Large Animal Model In Vivo

Tim R. Glowka¹, Pascal Paschenda², Michael Czaplik³, Jörg C. Kalff¹, René H. Tolba²

¹Department of Surgery, University of Bonn, ²Institute for Laboratory Animal Science & Experimental Surgery, RWTH Aachen University, ³Department of Anesthesiology, RWTH Aachen University

Adeno-Associated Virus-Mediated Delivery of CRISPR for Cardiac Gene Editing in Mice

Li Xu¹, Yandi Gao¹, Yeh Siang Lau¹, Renzhi Han¹

¹Department of Surgery, Davis Heart and Lung Research Institute, Biomedical Sciences Graduate Program, Biophysics Graduate Program, Ohio State University Wexner Medical Center

Invasive Hemodynamic Characterization of the Portal-hypertensive Syndrome in Cirrhotic Rats

Philipp Königshofer^1,2, Ksenia Brusilovskaya^1,2, Philipp Schwabl^1,2, Bruno K. Podesser³, Michael Trauner¹, Thomas Reiberger^1,2

¹Div. of Gastroenterology and Hepatology, Dept. of Internal Medicine III, Medical University of Vienna, Vienna, Austria, ²Vienna Hepatic Hemodynamic Laboratory, Medical University of Vienna, Vienna, Austria, ³Center of Biomedical Research, Medical University of Vienna, Vienna, Austria

Bedside Ultrasound for Guiding Fluid Removal in Patients with Pulmonary Edema: The Reverse-FALLS Protocol

Danielle Nichole O’Hara¹, Vikram Chabra², Sahar Ahmad²

¹Stony Brook University School of Medicine, ²Stony Brook University Medical Center, Stony Brook University School of Medicine

Isolation of Atrial Cardiomyocytes from a Rat Model of Metabolic Syndrome-related Heart Failure with Preserved Ejection Fraction

David Bode^1,2, Tim Guthof¹, Burkert M. Pieske^1,2, Frank R. Heinzel^1,2, Felix Hohendanner^1,2

¹Department of Internal Medicine and Cardiology, Charité University Medicine, ²German Center for Cardiovascular Research (DZHK)

Quantitative Micro-CT Analysis of Aortopathy in a Mouse Model of β-aminopropionitrile-induced Aortic Aneurysm and Dissection

Brittany O. Aicher¹, Subhradip Mukhopadhyay¹, Xin Lu², Selen C. Muratoglu¹, Dudley K. Strickland¹, Areck A. Ucuzian^1,3

¹Center for Vascular and Inflammatory Diseases, University of Maryland School of Medicine, ²Department of Diagnostic Radiology and Nuclear Medicine, University of Maryland School of Medicine, ³Division of Vascular Surgery, University of Maryland School of Medicine

Image-Guided Resection of Glioblastoma and Intracranial Implantation of Therapeutic Stem Cell-seeded Scaffolds

Kevin T. Sheets¹, Juli R. Bagó¹, Ivory L. Paulk¹, Shawn D. Hingtgen^1,2

¹Division of Pharmacoengineering and Molecular Pharmaceutics, UNC Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, ²Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill

Home-Based Transcranial Direct Current Stimulation Device Development: An Updated Protocol Used at Home in Healthy Subjects and Fibromyalgia Patients

Fabiana Carvalho^1,2, Aline Patrícia Brietzke^1,2, Assunta Gasparin^1,2, Franciele Pereira dos Santos^2,3, Rafael Vercelino⁴, Rafael Firmino Ballester^2,3, Paulo Roberto Stefani Sanches⁵, Danton Pereira da Silva Jr⁵, Iraci L. S. Torres^1,6, Felipe Fregni^7,8, Wolnei Caumo^1,2,9,10

¹Post-Graduate Program in Medical Sciences, School of Medicine, Universidade Federal do Rio Grande do Sul (UFRGS), ²Laboratory of Pain & Neuromodulation, Hospital de Clínicas de Porto Alegre (HCPA), ³School of Medicine, Universidade Federal do Rio Grande do Sul (UFRGS), ⁴Faculdade de Desenvolvimento do Rio Grande do Sul (FADERGS), Health and Wellness School Laureate International Universities, ⁵Biomedical Engineering Department, Hospital de Clínicas de Porto Alegre (HCPA), ⁶Pharmacology Department, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), ⁷Department of Physical Medicine and Rehabilitation, Harvard Medical School, ⁸Spaulding Neuromodulation Center, Spaulding Rehabilitation Hospital, ⁹Pain and Palliative Care Service, Hospital de Clínicas de Porto Alegre (HCPA), ¹⁰Department of Surgery, School of Medicine, Universidade Federal do Rio Grande do Sul (UFRGS)

A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation

Guihao Chen¹, Yuejin Yang¹, Chuansheng Xu², Shuo Gao²

¹State Key Laboratory of Cardiovascular Disease, Department of Cardiology, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, ²State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College

Murine Distal Colostomy, A Novel Model of Diversion Colitis in C57BL/6 Mice

Annabel Kleinwort¹, Paula Döring², Christine Hackbarth¹, Maciej Patrzyk¹, Claus-Dieter Heidecke¹, Tobias Schulze¹

¹Department of General Surgery, Visceral, Thoracic and Vascular Surgery, Universitätsmedizin Greifswald, ²Institute of Pathology, Universitätsmedizin Greifswald

Corneal Epithelial Abrasion with Ocular Burr As a Model for Cornea Wound Healing

Solja Kalha¹, Alison Kuony¹, Frederic Michon^1,2

¹Helsinki Institute of Life Science, Institute of Biotechnology, University of Helsinki, ²School of Medicine and Institute for Science and Technology in Medicine, Keele University

Assessment of Respiratory Function in Conscious Mice by Double-chamber Plethysmography

Samuel Mailhot-Larouche¹, Louis Deschênes¹, Katherine Lortie¹, Morgan Gazzola¹, David Marsolais¹, David Brunet², Annette Robichaud², Ynuk Bossé¹

¹Institut Universitaire de Cardiologie et de Pneumologie de Québec, Université Laval, ²SCIREQ Scientific Respiratory Equipment Inc.

Objective Nociceptive Assessment in Ventilated ICU Patients: A Feasibility Study Using Pupillometry and the Nociceptive Flexion Reflex

Davina Wildemeersch¹, Jens Gios², Philippe G. Jorens³, Guy H. Hans²

¹Multidisciplinary Pain Center, Anesthesiology and Critical Care Medicine, Antwerp University Hospital (UZA), University of Antwerp (UA), ²Multidisciplinary Pain Center (PCT), Antwerp University Hospital (UZA), ³Critical Care Medicine, Antwerp University Hospital (UZA), University of Antwerp (UA)

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