JoVE Journal

Medicine

JoVE Medicine includes methods and experimental approaches for biomedical research and clinical medicine, including case studies, clinical procedures, surgeries, clinical trials, and animal models of disease.

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A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma

Wenting Luo*^1,2,3,4, Guichang Pan*⁵, Huimin Huang^1,2,3,4, Peiyan Zheng^1,2,3,4, Nili Wei^1,2,3,4, Yifei Zhang⁵, Guangqiao Zeng^1,2,3,4, Baoqing Sun^1,2,3,4

¹State Key Laboratory of Respiratory Disease, ²National Clinical Research Center of Respiratory Disease, ³Guangzhou Institute of Respiratory Diseases, ⁴Department of Allergy and Clinical Immunology, First Affiliated Hospital of Guangzhou Medical University, ⁵Department of Respiratory Medicine, Sixth Affiliated Hospital of Guangzhou Medical University

JoVE 55723

Preparation of Herbal Medicine: Er-Xian Decoction and Er-Xian-containing Serum for In Vivo and In Vitro Experiments

Shufen Liu¹, Yueli Sun¹, Ji Li¹, Jingcheng Dong², Qin Bian^1,2

¹Longhua Hospital, Shanghai University of Traditional Chinese Medicine, ²The Academy of Integrative Medicine of Fudan University

JoVE 55654

Quantitative Visualization of Leukocyte Infiltrate in a Murine Model of Fulminant Myocarditis by Light Sheet Microscopy

Linda Männ¹, Anika Klingberg², Matthias Gunzer², Mike Hasenberg³

¹Department of Translational Skin Cancer Research, University of Duisburg/Essen, ²Institute for Experimental Immunology and Imaging, University of Duisburg/Essen, ³Imaging Center Essen, Electron Microscopy Unit, University Hospital of Essen

JoVE 55450

An Anesthesia, Surgery, and Harvest Method for the Evaluation of Transpedicular Screws Using an In Vivo Porcine Lumbar Spine Model

Young Jae Moon¹, Jong-Kil Kim², Hong-Geun Oh³, Ji-Hun Kang⁴, Gun-Joo Park¹, Kwang-Bok Lee¹

¹Department of Orthopaedic Surgery, Chonbuk National University School of Medicine, Chonbuk National University Hospital, ²Department of Orthopaedic Surgery, University of Seonam College of Medicine, Presbyterian Medical Center, ³Wowanimal Hospital, ⁴Department of Emergency Medicine, Inje University Busan Paik Hospital

JoVE 55225

Establishment of a Clinic-based Biorepository

Sarah E. Belden¹, Chandana K. Uppalapati², Agnes S. Pascual³, McKale R. Montgomery³, Kathryn J. Leyva², Elizabeth E. Hull³, Richard L. Averitte¹

¹Affiliated Dermatology & Affiliated Laboratories, Midwestern University Osteopathic Postdoctoral Training Institute, Midwestern University, ²Department of Microbiology & Immunology, Arizona College of Osteopathic Medicine, Midwestern University, ³Biomedical Sciences Program, College of Health Sciences, Midwestern University

JoVE 55583

Automated Radiochemical Synthesis of [¹⁸F]3F4AP: A Novel PET Tracer for Imaging Demyelinating Diseases

Pedro Brugarolas¹, Mohammed Bhuiyan², Anna Kucharski², Richard Freifelder²

¹Department of Neurology, The University of Chicago, ²Department of Radiology, The University of Chicago

JoVE 55537

Assessment of Spontaneous Alternation, Novel Object Recognition and Limb Clasping in Transgenic Mouse Models of Amyloid-β and Tau Neuropathology

Christian J. Miedel¹, Jennifer M. Patton¹, Andrew N. Miedel¹, Edward S. Miedel¹, Jonathan M. Levenson²

¹Hilltop Laboratory Animals, ²Preclinical Research & Development, Proclara Biosciences, Inc.

JoVE 55523

Ovine Lumbar Intervertebral Disc Degeneration Model Utilizing a Lateral Retroperitoneal Drill Bit Injury

Kai-Zheong Lim*¹, Christopher D. Daly*^1,2,3, Peter Ghosh⁴, Graham Jenkin³, David Oehme⁵, Justin Cooper-White^6,7, Taryn Naidoo⁶, Tony Goldschlager^1,8

¹Department of Surgery, Monash University, ²Department of Neurosurgery, Monash University, ³The Ritchie Centre, Hudson Institute of Medical Research, ⁴Proteobioactives, Pty Ltd, ⁵Department of Neurosurgery, St Vincent's Hospital, ⁶Australian Institute for Bioengineering and Nanotechnology, University of Queensland, ⁷School of Chemical Engineering, University of Queensland, ⁸Department of Neurosurgery, Monash Health

JoVE 55753

Comparing the Effects of Electronic Cigarette Vapor and Cigarette Smoke in a Novel In Vivo Exposure System

Anthony N. Hage¹, Will Krause¹, Angela Mathues¹, Luke Krasner², Seth Kasten¹, Jonathan L. Eliason^1,3, Abhijit Ghosh¹

¹Jobst Vascular Research Laboratory, University of Michigan Medical School, ²Department of Engineering, Purdue University, ³Department of Surgery, Section of Vascular Surgery, University of Michigan Health System

JoVE 55672

Full-root Aortic Valve Replacement by Stentless Aortic Xenografts in Patients with Small Aortic Roots

Reza Tavakoli^1,2, Peiman Jamshidi³, Max Gassmann^1,2

¹Institute of Veterinary Physiology, University of Zurich, ²Zurich Center of Integrative Human Physiology, University of Zurich, ³Department of Cardiology, Canton Hospital Lucerne

JoVE 55632

Uncontrolled Hemorrhagic Shock Modeled via Liver Laceration in Mice with Real Time Hemodynamic Monitoring

Mitchell Dyer¹, Shannon Haldeman¹, Andres Gutierrez¹, Lauryn Kohut¹, Anirban Sen Gupta², Matthew D. Neal¹

¹Department of Surgery, University of Pittsburgh, ²Department of Biomedical Engineering, Case Western Reserve University

JoVE 55554

Evaluating the Procedure for Performing Awake Cystometry in a Mouse Model

Travis K. Mann-Gow*¹, Troy R. Larson*¹, Chrissie T. Wøien², Thomas M. Andersen², Karl-Erik Andersson^3,4, Peter Zvara^1,2

¹Department of Surgery, University of Vermont, ²Department of Urology and Biomedical Laboratory, University of Southern Denmark, ³Wake Forest Institute for Regenerative Medicine, Wake Forest University, ⁴Institute for Clinical Medicine, Aarhus University

JoVE 55588

Subcutaneous Trigeminal Nerve Field Stimulation for Refractory Facial Pain

Martin Jakobs¹, Sigrid Schuh-Hofer², Andreas Unterberg¹, Rezvan Ahmadi¹

¹Department of Neurosurgery, University Hospital Heidelberg, ²Center for Biomedicine and Medical Technology, University Hospital Mannheim

JoVE 55408

Expression of Exogenous Cytokine in Patient-derived Xenografts via Injection with a Cytokine-transduced Stromal Cell Line

Jacqueline S. Coats¹, Ineavely Baez¹, Cornelia Stoian¹, Terry-Ann M. Milford², Xiaobing Zhang³, Olivia L. Francis¹, Ruijun Su¹, Kimberly J. Payne¹

¹Department of Pathology and Human Anatomy, Loma Linda University, ²Department of Basic Sciences, Loma Linda University, ³Department of Medicine, Loma Linda University

JoVE 55384

Laparoscopic Technique for Serial Collection of Liver and Mesenteric Lymph Nodes in Macaques

Alexander S. Zevin¹, Cassie Moats², Drew May², Solomon Wangari², Charlene Miller¹, Joel Ahrens², Naoto Iwayama², Megan Brown², Debbie Bratt², Nichole R. Klatt¹, Jeremy Smedley²

¹Department of Pharmaceutics, Washington National Primate Research Center, University of Washington, ²Division of Primate Resources, Washington National Primate Research Center, University of Washington

JoVE 55617

Use of a Central Venous Line for Fluids, Drugs and Nutrient Administration in a Mouse Model of Critical Illness

Sarah Derde¹, Steven Thiessen¹, Chloë Goossens¹, Thomas Dufour¹, Greet Van den Berghe¹, Lies Langouche¹

¹Clinical Division and Laboratory of Intensive Care Medicine, Department of Cellular and Molecular Medicine, KU Leuven

JoVE 55553

Determining Glucose Metabolism Kinetics Using ¹⁸F-FDG Micro-PET/CT

Blake J. Cochran¹, William J. Ryder^2,3,4,5, Arvind Parmar⁶, Kerstin Klaeser^4,5, Anthonin Reilhac⁷, Georgios I. Angelis^4,5, Steven R. Meikle^4,5, Philip J. Barter^1,5, Kerry-Anne Rye^1,5

¹School of Medical Sciences, Faculty of Medicine, UNSW Australia, ²Department of Nuclear Medicine, Concord Hospital, ³National Imaging Facility, University of Sydney, ⁴Brain and Mind Centre, University of Sydney, ⁵Faculty of Health Sciences, University of Sydney, ⁶Life Sciences, ANSTO, ⁷CERMEP

JoVE 55184

Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells

Natalia Voronina¹, Heiko Lemcke¹, Frank Wiekhorst², Jens-Peter Kühn³, Markus Frank⁴, Gustav Steinhoff¹, Robert David¹

¹Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, ²Physikalisch-Technische Bundesanstalt, ³Department of Radiology and Neuroradiology, Ernst-Moritz-Arndt-University Greifswald, ⁴Electron Microscopy Center, University of Rostock

JoVE 55567

Nerve-sparing Mid-urethral Obstruction (NeMO) in Female Small Rodents

Martin Sidler^1,2,3, Karen J. Aitken¹, Jia Xin Jiang⁴, Darius J. Bägli^1,2,3

¹Developmental and Stem Cell Biology, Research Institute, The Hospital for Sick Children, ²Institute of Medical Science, University of Toronto, ³Pediatric Urology, The Hospital for Sick Children, ⁴Molecular Structure and Function, Research Institute, The Hospital for Sick Children

JoVE 55288

Direct Re-implantation of Left Coronary Artery into the Aorta in Adults with Anomalous Origin of Left Coronary Artery from the Pulmonary Artery (ALCAPA)

Reza Tavakoli¹, Peiman Jamshidi², Nassrin Yamani³, Max Gassmann¹

¹Institute of Veterinary Physiology and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, ²Department of Cardiology, Canton Hospital Lucerne, ³Department of Radiology, Canton Hospital Lucerne

JoVE 55590

A Method for Quantifying Upper Limb Performance in Daily Life Using Accelerometers

Catherine E. Lang^1,2,3, Kimberly J. Waddell¹, Joseph W. Klaesner^1,4,5, Marghuretta D. Bland^1,2,3

¹Program in Physical Therapy, Washington University School of Medicine, ²Program in Occupational Therapy, Washington University School of Medicine, ³Department of Neurology, Washington University School of Medicine, ⁴Mallinckrodt Institute of Radiology, Washington University School of Medicine, ⁵Department of Biomedical Engineering, Washington University

JoVE 55673

Transient Middle Cerebral Artery Occlusion Model of Neonatal Stroke in P10 Rats

Amara Larpthaveesarp¹, Fernando F. Gonzalez¹

¹Department of Pediatrics, University of California, San Francisco

JoVE 54830

Repeated Measurement of Respiratory Muscle Activity and Ventilation in Mouse Models of Neuromuscular Disease

Victoria N. Jensen*¹, Shannon H. Romer*², Sarah M. Turner³, Steven A. Crone³

¹Neuroscience Graduate Program, University of Cincinnati, ²Department of Biological Sciences, Wright State University, ³Division of Neurosurgery, Cincinnati Children's Hospital Medical Center

JoVE 55599

3D Whole-heart Myocardial Tissue Analysis

Hans Thijs Van den Broek¹, Leon De Jong^1,2, Pieter A. Doevendans¹, Steven A.J. Chamuleau¹, Frebus J. Van Slochteren*¹, René Van Es*¹

¹Department of Cardiology, Division Heart and Lungs, University Medical Center Utrecht, ²MIRA Institute, University Twente

JoVE 54974

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Bioengineering

Biology

Cancer Research

Immunology and Infection

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