JoVE Journal

Neuroscience

JoVE Neuroscience includes methods and techniques for studying the brain and nervous system; also featuring potential treatments for neurological conditions and diseases.

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Real-Time fMRI Brain Mapping in Animals

Sangcheon Choi^1,2, Kengo Takahashi^1,2, Yuanyuan Jiang^1,3, Sascha Köhler⁴, Hang Zeng^1,2, Qi Wang^1,2, Yan Ma^1,2, Xin Yu^1,3

¹Max Planck Institute for Biological Cybernetics, ²Graduate Training Centre of Neuroscience, ³MGH/MIT/HMS Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Harvard Medical School, Massachusetts General Hospital, ⁴Bruker BioSpin

JoVE 61463

Methods Collection

Horizontal Hippocampal Slices of the Mouse Brain

Evelien Van Hoeymissen^1,2, Koenraad Philippaert², Rudi Vennekens², Joris Vriens*¹, Katharina Held*^1,2

¹Laboratory of Endometrium, Endometriosis and Reproductive Medicine, Department of Development and Regeneration, KU Leuven, ²Laboratory of Ion Channel Research, VIB-KU Leuven Center for Brain and Disease Research, Leuven, Belgium and Department of Molecular Medicine, KU Leuven

JoVE 61753

Methods Collection

Evaluating Postural Control and Lower-extremity Muscle Activation in Individuals with Chronic Ankle Instability

Lulu Yin¹, Zhangqi Lai², Xiaoyue Hu², Kun Liu³, Lin Wang²

¹Department of Critical Care Medicine, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, ²School of Kinesiology, Shanghai University of Sport, ³Department of Rehabilitation Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital

JoVE 61592

Methods Collection

Quantitative Approaches for Scoring in vivo Neuronal Aggregate and Organelle Extrusion in Large Exopher Vesicles in C. elegans

Meghan Lee Arnold*¹, Jason Cooper*¹, Barth D. Grant¹, Monica Driscoll¹

¹Department of Molecular Biology and Biochemistry, Rutgers University

JoVE 61368

Methods Collection

Implantation of Osmotic Pumps and Induction of Stress to Establish a Symptomatic, Pharmacological Mouse Model for DYT/PARK-ATP1A3 Dystonia

Lisa Rauschenberger¹, Susanne Knorr¹, Jens Volkmann¹, Chi Wang Ip¹

¹Department of Neurology, University Hospital of Würzburg

JoVE 61635

Preparing Acute Brain Slices from the Dorsal Pole of the Hippocampus from Adult Rodents

Sam A. Booker¹

¹Simons Initiative for the Developing Brain, Centre for Discovery Brain Sciences, The University of Edinburgh

JoVE 61699

Methods Collection

Quantifying Spontaneous Ca²⁺ Fluxes and their Downstream Effects in Primary Mouse Midbrain Neurons

Eric A. Bancroft¹, Rahul Srinivasan^1,2

¹Department of Neuroscience and Experimental Therapeutics, Texas A&M University Health Science Center, College of Medicine, ²Texas A&M Institute for Neuroscience

JoVE 61481

Methods Collection

Combined In Vivo Anatomical and Functional Tracing of Ventral Tegmental Area Glutamate Terminals in the Hippocampus

Amita Shrestha¹, Philip A. Adeniyi¹, Olalekan M. Ogundele¹

¹Department of Comparative Biomedical Sciences, Louisiana State University School of Veterinary Medicine

JoVE 61282

Sheath-Preserving Optic Nerve Transection in Rats to Assess Axon Regeneration and Interventions Targeting the Retinal Ganglion Cell Axon

Jiun L. Do¹, Salam Allahwerdy¹, Ryan C. David¹, Robert N. Weinreb¹, Derek S. Welsbie¹

¹Hamilton Glaucoma Center, Viterbi Family Department of Ophthalmology, Shiley Eye Institute, University of California San Diego

JoVE 61748

Unraveling the Role of Discrete Areas of the Rat Brain in the Regulation of Ovulation through Reversible Inactivation by Tetrodotoxin Microinjections

Carlos-Camilo Silva*^1,2, Montserrat Bolaños-Hurtado*¹, Cinthia Juárez-Tapia⁴, Angélica Flores^1,2, Isabel Arrieta-Cruz^3,5, María-Esther Cruz³, Roberto Domínguez^1,2,3

¹Chronobiology of Reproduction Research Lab, Biology of Reproduction Research Unit, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, ²Developmental Biology Lab, Biology of Reproduction Research Unit, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, ³Neuroendocrinology Lab, Biology of Reproduction Research Unit, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, ⁴Facultad de Ciencias, Universidad Nacional Autónoma de México, ⁵Department of Basic Research, Instituto Nacional de Geriatria, Ministry of Health

JoVE 61493

Intracerebroventricular Treatment with Resiniferatoxin and Pain Tests in Mice

Akihiro Fukushima^1,2, Moeko Fujii¹, Hideki Ono^1,2

¹Laboratory of Clinical Pharmacy and Pharmacology, Musashino University, ²Research Institute of Pharmaceutical Sciences, Musashino University

JoVE 57570

Imaging and Analysis of Neurofilament Transport in Excised Mouse Tibial Nerve

Nicholas P. Boyer¹, Maite Azcorra^1,2, Peter Jung^3,4, Anthony Brown¹

¹Department of Neuroscience, The Ohio State University, ²Present address: Interdepartmental Neuroscience Graduate Program and Department of Neurobiology, Northwestern University, ³Quantitative Biology Institute, Ohio University, ⁴Department of Physics and Astronomy, Ohio University

JoVE 61264

Methods Collection

Obtaining Human Microglia from Adult Human Brain Tissue

Ishan Agrawal¹, Shivanjali Saxena¹, Preethika Nair¹, Deepak Jha², Sushmita Jha¹

¹Department of Bioscience and Bioengineering, Indian Institute of Technology Jodhpur, ²Department of Neurosurgery, All India Institute of Medical Sciences Jodhpur

JoVE 61438

Double Direct Injection of Blood into the Cisterna Magna as a Model of Subarachnoid Hemorrhage

Martin Pedard¹, Mohamad El Amki¹, Antoine Lefevre-Scelles^1,2, Vincent Compère^1,2, Hélène Castel¹

¹University of Rouen Normandy, INSERM U1239, DC2N, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France, ²Department of Anesthesiology and Critical Care, Rouen University Hospital, Rouen, France

JoVE 61322

Acute Mouse Brain Slicing to Investigate Spontaneous Hippocampal Network Activity

Alexander C. Whitebirch¹

¹Department of Neuroscience, Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University

JoVE 61704

Methods Collection

Detrusor Underactivity Model in Rats by Conus Medullaris Transection

Xin Zheng*¹, Menghua Wu*¹, Jian Song², Jimao Zhao²

¹Department of Urology, Beijing YouAn Hospital, Capital Medical University, ²Department of Urology, Beijing Friendship Hospital, Capital Medical University

JoVE 61576

Establishment of Acute Pontine Infarction in Rats by Electrical Stimulation

Ming Luo¹, Xiangyue Tang¹, Juehua Zhu², Zhihua Qiu¹, Yongjun Jiang¹

¹The Second Affiliated Hospital of Guangzhou Medical University, ²Department of Neurology, The First Affiliated Hospital of Soochow University

JoVE 60783

Successful In vivo Calcium Imaging with a Head-Mount Miniaturized Microscope in the Amygdala of Freely Behaving Mouse

Han-Sol Lee^1,2, Jin-Hee Han^1,2

¹Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), ²KAIST Institute for BioCentury (KIB), Korea Advanced Institute of Science and Technology (KAIST)

JoVE 61659

An Emerging Target Paradigm to Evoke Fast Visuomotor Responses on Human Upper Limb Muscles

Rebecca A. Kozak^1,4, Aaron L. Cecala^2,4, Brian D. Corneil^1,2,3,4

¹Graduate Program in Neuroscience, Western University, ²Department of Physiology and Pharmacology, Western University, ³Department of Psychology, Western University, ⁴Robarts Research Institute

JoVE 61428

In Vitro Wedge Slice Preparation for Mimicking In Vivo Neuronal Circuit Connectivity

Matthew J. Fischl¹, Catherine J. C. Weisz¹

¹Section on Neuronal Circuitry, National Institute on Deafness and Other Communication Disorders, NIH

JoVE 61664

Brain Morphology of Cannabis Users With or Without Psychosis: A Pilot MRI Study

Giuseppe Delvecchio*¹, Lucio Oldani*², Gian Mario Mandolini², Alessandro Pigoni³, Valentina Ciappolino², Giandomenico Schiena², Matteo Lazzaretti², Elisabetta Caletti², Viviana Barbieri¹, Claudia Cinnante⁴, Fabio Triulzi^1,4, Paolo Brambilla^1,2