JoVE Journal

Neuroscience

JoVE Neuroscience includes methods and techniques for studying the brain and nervous system; also featuring potential treatments for neurological conditions and diseases.

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Construction of an Improved Multi-Tetrode Hyperdrive for Large-Scale Neural Recording in Behaving Rats

Li Lu¹, Briana Popeney¹, J. David Dickman¹, Dora E. Angelaki¹

¹Department of Neuroscience, Baylor College of Medicine

Preparing Fresh Retinal Slices from Adult Zebrafish for Ex Vivo Imaging Experiments

Michelle M. Giarmarco¹, Whitney M. Cleghorn¹, James B. Hurley^1,2, Susan E. Brockerhoff^1,2

¹Department of Biochemistry, University of Washington, ²Department of Ophthalmology, University of Washington

Novel Passive Clearing Methods for the Rapid Production of Optical Transparency in Whole CNS Tissue

Jiwon Woo^1,2,3, Eunice Yoojin Lee⁴, Hyo-Suk Park^1,3, Jeong Yoon Park^1,3, Yong Eun Cho^1,2,3

¹Department of Neurosurgery, Gangnam Severance Hospital, Yonsei University College of Medicine, ²Brain Korea 21 PLUS Project for Medical Science, Yonsei University, ³The Spine and Spinal Cord Institute, Biomedical Center, Gangnam Severance Hospital, Yonsei University College of Medicine, ⁴Columbia University College of Physicians and Surgeons

A Simple and Reproducible Method to Prepare Membrane Samples from Freshly Isolated Rat Brain Microvessels

Hrvoje Brzica*¹, Wazir Abdullahi*¹, Bianca G. Reilly¹, Patrick T. Ronaldson¹

¹Department of Pharmacology, College of Medicine, University of Arizona, Tucson

Making, Testing, and Using Potassium Ion Selective Microelectrodes in Tissue Slices of Adult Brain

J. Christopher Octeau¹, Guido Faas², Istvan Mody^1,2, Baljit S. Khakh^1,3

¹Department of Physiology, David Geffen School of Medicine, University of California Los Angeles, ²Department of Neurology, David Geffen School of Medicine, University of California Los Angeles, ³Department of Neurobiology, David Geffen School of Medicine, University of California Los Angeles

Levator Auris Longus Preparation for Examination of Mammalian Neuromuscular Transmission Under Voltage Clamp Conditions

Steven R. A. Burke¹, Eric J. Reed¹, Shannon H. Romer¹, Andrew A. Voss¹

¹Department of Biological Sciences, Wright State University

Neurovascular Network Explorer 2.0: A Simple Tool for Exploring and Sharing a Database of Optogenetically-evoked Vasomotion in Mouse Cortex In Vivo

Hana Uhlirova^1,2, Peifang Tian^3,4, Kıvılcım Kılıç^3,5, Martin Thunemann¹, Vishnu B. Sridhar⁶, Radim Chmelik^2,7, Hauke Bartsch¹, Anders M. Dale^1,3, Anna Devor^1,3,8, Payam A. Saisan³

¹Department of Radiology, University of California, San Diego, ²Central European Institute of Technology, Brno University of Technology, ³Department of Neurosciences, University of California, San Diego, ⁴Department of Physics, John Carroll University, ⁵Department of Biomedical Engineering, Boston University, ⁶Bioengineering Undergraduate Program, University of California, San Diego, ⁷Institute of Physical Engineering, Faculty of Mechanical Engineering, Brno University of Technology, ⁸Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School

Mitochondrial Ca²⁺ Retention Capacity Assay and Ca²⁺-triggered Mitochondrial Swelling Assay

Wei Li^1,2, Chen Zhang¹, Xiulian Sun³

¹Department of Neurology, Qilu Hospital of Shandong University, ²Department of Neurology, Qingdao Municipal Hospital, ³Brain Research Institute, Qilu Hospital of Shandong University

A Microbiomechanical System for Studying Varicosity Formation and Recovery in Central Neuron Axons

Dustin Servello¹, Yuanzheng Gu^2,3, Chen Gu^1,2

¹Molecular, Cellular, and Developmental Biology Program, The Ohio State University, ²Department of Biological Chemistry and Pharmacology, The Ohio State University, ³Biogen

Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains

Julie Mazzolini¹, Kelda Chia¹, Dirk Sieger¹

¹Centre for Discovery Brain Sciences, University of Edinburgh

Establishing Mouse Models for Zika Virus-induced Neurological Disorders Using Intracerebral Injection Strategies: Embryonic, Neonatal, and Adult

Stephanie A. Herrlinger¹, Qiang Shao², Li Ma², Melinda Brindley³, Jian-Fu Chen²

¹Biomedical and Health Sciences Institute, University of Georgia, ²Center for Craniofacial Molecular Biology, University of Southern California, ³Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia

An In Ovo Model for Testing Insulin-mimetic Compounds

Renate Haselgrübler*¹, Flora Stübl*¹, Verena Stadlbauer^1,2, Peter Lanzerstorfer¹, Julian Weghuber^1,2

¹University of Applied Sciences Upper Austria, ²Austrian Competence Center for Feed and Food Quality, Safety and Innovation

Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons

Lara Eid^1,2, Mathieu Lachance¹, Gilles Hickson^1,3, Elsa Rossignol^1,2,4

¹Centre de recherche du CHU Sainte-Justine, ²Department of Neuroscience, Université de Montréal, ³Department of pathology and cellular biology, Université de Montréal, ⁴Department of Pediatrics, Université de Montréal

Method for High Speed Stretch Injury of Human Induced Pluripotent Stem Cell-derived Neurons in a 96-well Format

Jack K. Phillips¹, Sydney A. Sherman^1,2, Sevan R. Oungoulian³, John D. Finan¹

¹Department of Neurosurgery, NorthShore University HealthSystem, ²Midwestern University/Chicago College of Osteopathic Medicine, ³Independent Contractor

Identifying Transcription Factor Olig2 Genomic Binding Sites in Acutely Purified PDGFRα+ Cells by Low-cell Chromatin Immunoprecipitation Sequencing Analysis

Xiaomin Dong¹, Raquel Cuevas-Diaz Duran¹, Yanan You¹, Jia Qian Wu¹

¹The Vivian L. Smith Department of Neurosurgery, Center for Stem Cell and Regenerative Medicine, University of Texas Health Science Center

Improving the Application of High Molecular Weight Biotinylated Dextran Amine for Thalamocortical Projection Tracing in the Rat

Dongsheng Xu*¹, Jingjing Cui*¹, Jia Wang¹, Zhiyun Zhang¹, Chen She¹, Wanzhu Bai¹

¹Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences

An Alternative Approach to Study Primary Events in Neurodegeneration Using Ex Vivo Rat Brain Slices

Emanuele Brai¹, Antonella Cogoni¹, Susan A. Greenfield¹

¹Neuro-Bio Ltd, Building F5, Culham Science Centre

Simultaneous Recordings of Cortical Local Field Potentials, Electrocardiogram, Electromyogram, and Breathing Rhythm from a Freely Moving Rat

Yu Shikano¹, Takuya Sasaki¹, Yuji Ikegaya^1,2

¹Graduate School of Pharmaceutical Sciences, The University of Tokyo, ²Center for Information and Neural Networks

Using Brain Activation (nir-HEG/Q-EEG) and Execution Measures (CPTs) in a ADHD Assessment Protocol

Debora Areces¹, Marisol Cueli¹, Trinidad García¹, Paloma González-Castro¹, Celestino Rodríguez¹

¹Department of Psychology, Oviedo University

Neuron-Macrophage Co-cultures to Activate Macrophages Secreting Molecular Factors with Neurite Outgrowth Activity

Hyeok Jun Yun^1,2, Eun-Hye Kim^1,2, Byung Gon Kim^1,2,3

¹Department of Brain Science, Ajou University School of Medicine, ²Neuroscience Graduate Program, Department of Biomedical Sciences, Ajou University School of Medicine, ³Department of Neurology, Ajou University School of Medicine

Kinematic Analysis Using 3D Motion Capture of Drinking Task in People With and Without Upper-extremity Impairments

Margit Alt Murphy^1,3, Steve Murphy², Hanna C. Persson^1,3, Ulla-Britt Bergström³, Katharina Stibrant Sunnerhagen¹

¹Institution of Neuroscience and Physiology, Rehabilitation Medicine, Sahlgrenska Academy, University of Gothenburg, ²Securesoft Sweden, ³Departement of Occupational Therapy and Physiotherapy, Sahlgrenska University Hospital

Visualization of Thalamocortical Axon Branching and Synapse Formation in Organotypic Cocultures

Naoyuki Matsumoto^1,2, Nobuhiko Yamamoto²

¹Department of Medical Neuroscience, Graduate School of Medical Sciences, Kanazawa University, ²Neuroscience Laboratories, Graduate School of Frontier Biosciences, Osaka University

Identification of Dopamine D1-Alpha Receptor Within Rodent Nucleus Accumbens by an Innovative RNA In Situ Detection Technology

Hailong Li¹, Jessica M. Illenberger¹, Kristen A. McLaurin¹, Charles F. Mactutus¹, Rosemarie M. Booze¹

¹Program in Behavioral Neuroscience, Department of Psychology, University of South Carolina

High-resolution Volume Imaging of Neurons by the Use of Fluorescence eXclusion Method and Dedicated Microfluidic Devices

Céline Braïni¹, Angelo Mottolese¹, Ivan Ferrante¹, Sylvain Monnier², Catherine Villard¹

¹Laboratoire Physico-Chimie Curie, Institut Curie, Institut Pierre-Gilles de Gennes pour la microfluidique, Université PSL, CNRS, ²UMR 144 Institut Curie, Université PSL, CNRS

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