JoVE Journal

Neuroscience

JoVE Neuroscience includes methods and techniques for studying the brain and nervous system; also featuring potential treatments for neurological conditions and diseases.

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An In Ovo Model for Testing Insulin-mimetic Compounds

Renate Haselgrübler*¹, Flora Stübl*¹, Verena Stadlbauer^1,2, Peter Lanzerstorfer¹, Julian Weghuber^1,2

¹University of Applied Sciences Upper Austria, ²Austrian Competence Center for Feed and Food Quality, Safety and Innovation

Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons

Lara Eid^1,2, Mathieu Lachance¹, Gilles Hickson^1,3, Elsa Rossignol^1,2,4

¹Centre de recherche du CHU Sainte-Justine, ²Department of Neuroscience, Université de Montréal, ³Department of pathology and cellular biology, Université de Montréal, ⁴Department of Pediatrics, Université de Montréal

Method for High Speed Stretch Injury of Human Induced Pluripotent Stem Cell-derived Neurons in a 96-well Format

Jack K. Phillips¹, Sydney A. Sherman^1,2, Sevan R. Oungoulian³, John D. Finan¹

¹Department of Neurosurgery, NorthShore University HealthSystem, ²Midwestern University/Chicago College of Osteopathic Medicine, ³Independent Contractor

Identifying Transcription Factor Olig2 Genomic Binding Sites in Acutely Purified PDGFRα+ Cells by Low-cell Chromatin Immunoprecipitation Sequencing Analysis

Xiaomin Dong¹, Raquel Cuevas-Diaz Duran¹, Yanan You¹, Jia Qian Wu¹

¹The Vivian L. Smith Department of Neurosurgery, Center for Stem Cell and Regenerative Medicine, University of Texas Health Science Center

Improving the Application of High Molecular Weight Biotinylated Dextran Amine for Thalamocortical Projection Tracing in the Rat

Dongsheng Xu*¹, Jingjing Cui*¹, Jia Wang¹, Zhiyun Zhang¹, Chen She¹, Wanzhu Bai¹

¹Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences

An Alternative Approach to Study Primary Events in Neurodegeneration Using Ex Vivo Rat Brain Slices

Emanuele Brai¹, Antonella Cogoni¹, Susan A. Greenfield¹

¹Neuro-Bio Ltd, Building F5, Culham Science Centre

Simultaneous Recordings of Cortical Local Field Potentials, Electrocardiogram, Electromyogram, and Breathing Rhythm from a Freely Moving Rat

Yu Shikano¹, Takuya Sasaki¹, Yuji Ikegaya^1,2

¹Graduate School of Pharmaceutical Sciences, The University of Tokyo, ²Center for Information and Neural Networks

Using Brain Activation (nir-HEG/Q-EEG) and Execution Measures (CPTs) in a ADHD Assessment Protocol

Debora Areces¹, Marisol Cueli¹, Trinidad García¹, Paloma González-Castro¹, Celestino Rodríguez¹

¹Department of Psychology, Oviedo University

Neuron-Macrophage Co-cultures to Activate Macrophages Secreting Molecular Factors with Neurite Outgrowth Activity

Hyeok Jun Yun^1,2, Eun-Hye Kim^1,2, Byung Gon Kim^1,2,3

¹Department of Brain Science, Ajou University School of Medicine, ²Neuroscience Graduate Program, Department of Biomedical Sciences, Ajou University School of Medicine, ³Department of Neurology, Ajou University School of Medicine

Kinematic Analysis Using 3D Motion Capture of Drinking Task in People With and Without Upper-extremity Impairments

Margit Alt Murphy^1,3, Steve Murphy², Hanna C. Persson^1,3, Ulla-Britt Bergström³, Katharina Stibrant Sunnerhagen¹

¹Institution of Neuroscience and Physiology, Rehabilitation Medicine, Sahlgrenska Academy, University of Gothenburg, ²Securesoft Sweden, ³Departement of Occupational Therapy and Physiotherapy, Sahlgrenska University Hospital

Visualization of Thalamocortical Axon Branching and Synapse Formation in Organotypic Cocultures

Naoyuki Matsumoto^1,2, Nobuhiko Yamamoto²

¹Department of Medical Neuroscience, Graduate School of Medical Sciences, Kanazawa University, ²Neuroscience Laboratories, Graduate School of Frontier Biosciences, Osaka University

Identification of Dopamine D1-Alpha Receptor Within Rodent Nucleus Accumbens by an Innovative RNA In Situ Detection Technology

Hailong Li¹, Jessica M. Illenberger¹, Kristen A. McLaurin¹, Charles F. Mactutus¹, Rosemarie M. Booze¹

¹Program in Behavioral Neuroscience, Department of Psychology, University of South Carolina

High-resolution Volume Imaging of Neurons by the Use of Fluorescence eXclusion Method and Dedicated Microfluidic Devices

Céline Braïni¹, Angelo Mottolese¹, Ivan Ferrante¹, Sylvain Monnier², Catherine Villard¹

¹Laboratoire Physico-Chimie Curie, Institut Curie, Institut Pierre-Gilles de Gennes pour la microfluidique, Université PSL, CNRS, ²UMR 144 Institut Curie, Université PSL, CNRS

An Objective and Reproducible Test of Olfactory Learning and Discrimination in Mice

Gary Liu*^1,2, Jay M. Patel*^2,3, Burak Tepe¹, Cynthia K. McClard^2,4, Jessica Swanson⁴, Kathleen B. Quast⁴, Benjamin R. Arenkiel^1,3,4,5

¹Program in Developmental Biology, Baylor College of Medicine, ²Medical Scientist Training Program, Baylor College of Medicine, ³Department of Neuroscience, Baylor College of Medicine, ⁴Department of Molecular and Human Genetics, Baylor College of Medicine, ⁵Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital

Tracking Drosophila Larval Behavior in Response to Optogenetic Stimulation of Olfactory Neurons

David A. Clark^1,2, Donovan Kohler¹, America Mathis¹, Eryn Slankster¹, Samipya Kafle¹, Seth R. Odell^1,2, Dennis Mathew^1,2

¹Department of Biology, MS-0314, University of Nevada, ²Integrated Neuroscience Graduate Program, University of Nevada

An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice

Nastasia K-H Lim^1,2, Visse Moestrup^3,4, Xiao Zhang¹, Wen-An Wang⁵, Arne Møller^3,4,6, Fu-De Huang^1,4

¹Shanghai Advanced Research Institute, University of Chinese Academy of Sciences, Chinese Academy of Science, ²Shanghai Institute of Materia Medica, University of Chinese Academy of Sciences, Chinese Academy of Science, ³Center of Functionally Integrative Neuroscience, Department of Clinical Medicine, Aarhus University, ⁴Sino-Danish Center for Education and Research (SDC), ⁵Department of Neurology, Xinhua Hospital Chongming Branch Affiliated to Shanghai Jiao Tong University School of Medicine, ⁶Department of Nuclear Medicine and PET-centre, Aarhus University Hospital

Preparations and Protocols for Whole Cell Patch Clamp Recording of Xenopus laevis Tectal Neurons

Zhenyu Liu¹, Katelynne B. Donnelly¹, Kara G. Pratt¹

¹Department of Zoology and Physiology and Program in Neuroscience, University of Wyoming

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices

Olivier Camiré^1,2, Lisa Topolnik^1,2

¹CHU de Québec-Université Laval Research Center, Université Laval, ²Department of Biochemistry, Microbiology and Bioinformatics, Université Laval

Combining Optogenetics with Artificial microRNAs to Characterize the Effects of Gene Knockdown on Presynaptic Function within Intact Neuronal Circuits

Agnes Thalhammer¹, Fanny Jaudon¹, Lorenzo A. Cingolani¹

¹Center for Synaptic Neuroscience and Technology, Istituto Italiano di Tecnologia

Monitoring Cell-to-cell Transmission of Prion-like Protein Aggregates in Drosophila Melanogaster

Kirby M. Donnelly¹, Margaret M. P. Pearce¹

¹Department of Biological Sciences, University of the Sciences

An In Vivo Assessment of Blood-Brain Barrier Disruption in a Rat Model of Ischemic Stroke

Hamdollah Panahpour¹, Mehdi Farhoudi², Yadollah Omidi³, Javad Mahmoudi²

¹Department of Physiology, Medical School, Ardabil University of Medical Sciences, ²Neurosciences Research Center, Tabriz University of Medical Sciences, ³Research Center for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences

Isolation and Culture of Rodent Microglia to Promote a Dynamic Ramified Morphology in Serum-free Medium

Hannah Y. Collins¹, Christopher J. Bohlen¹

¹Department of Neurobiology, Stanford University

Adult Mouse DRG Explant and Dissociated Cell Models to Investigate Neuroplasticity and Responses to Environmental Insults Including Viral Infection

Michele Fornaro¹, Harsh Sharthiya¹, Vaibhav Tiwari²

¹Department of Anatomy, Chicago College of Osteopathic Medicine (CCOM), Midwestern University, ²Department of Microbiology and Immunology, Chicago College of Osteopathic Medicine (CCOM), Midwestern University

Piezo High Accuracy Surgical Osteal Removal (PHASOR): A Technique for Improved Cranial Window Surgery in Mice

Samuel D. Clark¹, Rachel Mikofsky*¹, Jacqueline Lawson*¹, David Sulzer¹

¹Departments of Psychiatry, Neurology, & Pharmacology, Columbia University Medical Campus

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