JoVE Journal

Neuroscience

JoVE Neuroscience includes methods and techniques for studying the brain and nervous system; also featuring potential treatments for neurological conditions and diseases.

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Preparations and Protocols for Whole Cell Patch Clamp Recording of Xenopus laevis Tectal Neurons

Zhenyu Liu¹, Katelynne B. Donnelly¹, Kara G. Pratt¹

¹Department of Zoology and Physiology and Program in Neuroscience, University of Wyoming

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices

Olivier Camiré^1,2, Lisa Topolnik^1,2

¹CHU de Québec-Université Laval Research Center, Université Laval, ²Department of Biochemistry, Microbiology and Bioinformatics, Université Laval

Combining Optogenetics with Artificial microRNAs to Characterize the Effects of Gene Knockdown on Presynaptic Function within Intact Neuronal Circuits

Agnes Thalhammer¹, Fanny Jaudon¹, Lorenzo A. Cingolani¹

¹Center for Synaptic Neuroscience and Technology, Istituto Italiano di Tecnologia

Monitoring Cell-to-cell Transmission of Prion-like Protein Aggregates in Drosophila Melanogaster

Kirby M. Donnelly¹, Margaret M. P. Pearce¹

¹Department of Biological Sciences, University of the Sciences

An In Vivo Assessment of Blood-Brain Barrier Disruption in a Rat Model of Ischemic Stroke

Hamdollah Panahpour¹, Mehdi Farhoudi², Yadollah Omidi³, Javad Mahmoudi²

¹Department of Physiology, Medical School, Ardabil University of Medical Sciences, ²Neurosciences Research Center, Tabriz University of Medical Sciences, ³Research Center for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences

Isolation and Culture of Rodent Microglia to Promote a Dynamic Ramified Morphology in Serum-free Medium

Hannah Y. Collins¹, Christopher J. Bohlen¹

¹Department of Neurobiology, Stanford University

Adult Mouse DRG Explant and Dissociated Cell Models to Investigate Neuroplasticity and Responses to Environmental Insults Including Viral Infection

Michele Fornaro¹, Harsh Sharthiya¹, Vaibhav Tiwari²

¹Department of Anatomy, Chicago College of Osteopathic Medicine (CCOM), Midwestern University, ²Department of Microbiology and Immunology, Chicago College of Osteopathic Medicine (CCOM), Midwestern University

Piezo High Accuracy Surgical Osteal Removal (PHASOR): A Technique for Improved Cranial Window Surgery in Mice

Samuel D. Clark¹, Rachel Mikofsky*¹, Jacqueline Lawson*¹, David Sulzer¹

¹Departments of Psychiatry, Neurology, & Pharmacology, Columbia University Medical Campus

An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers

Kavi Devraj^1,2, Sylvaine Guérit¹, Jakranka Macas¹, Yvonne Reiss¹

¹Institute of Neurology (Edinger Institute), Goethe University Hospital, ²Pharmazentrum Frankfurt, Institute for General Pharmacology and Toxicology, Goethe University Hospital

Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method

Jonathan T. Ting*¹, Brian R. Lee*¹, Peter Chong¹, Gilberto Soler-Llavina¹, Charles Cobbs², Christof Koch¹, Hongkui Zeng¹, Ed Lein¹

¹Cell Types Program, Allen Institute for Brain Science, ²The Ben and Catherine Ivy Center for Advanced Brain Tumor Treatment, Swedish Neuroscience Institute

Retrograde Neuroanatomical Tracing of Phrenic Motor Neurons in Mice

Jean-Michel Vandeweerd¹, Fanny Hontoir¹, Alexis De Knoop¹, Kathleen De Swert², Charles Nicaise²

¹URVI, NARILIS, Université de Namur, ²URPhyM, NARILIS, Université de Namur

Monitoring the Effect of Osmotic Stress on Secretory Vesicles and Exocytosis

Hoda Fathali¹, Johan Dunevall¹, Soodabeh Majdi², Ann-Sofie Cans¹

¹Department of Chemistry and Chemical Engineering, Chalmers University of Technology, ²Department of Chemistry and Molecular Biology, University of Gothenburg

Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans

Holger Fehlauer*¹, Adam L. Nekimken*^1,2, Anna A. Kim^1,2, Beth L. Pruitt^1,2,3, Miriam B. Goodman^1,2, Michael Krieg⁴

¹Department of Molecular and Cellular Physiology, Stanford University, ²Department of Mechanical Engineering, Stanford University, ³Department of Bioengineering, Stanford University, ⁴Group of Neurophotonics and Mechanical Systems Biology, The Institute of Photonic Sciences (ICFO)

Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation

Jessica C. Stark*¹, Euan Wallace^1,2, Rebecca Lim¹, Bryan Leaw*¹

¹The Ritchie Centre, Hudson Institute of Medical Research, ²Department of Obstetrics & Gynaecology, Monash University

Examining Monosynaptic Connections in Drosophila Using Tetrodotoxin Resistant Sodium Channels

Xiaonan Zhang¹, Quentin Gaudry¹

¹Department of Biology, University of Maryland

Establishing a Mouse Model of a Pure Small Fiber Neuropathy with the Ultrapotent Agonist of Transient Receptor Potential Vanilloid Type 1

Yi-Chen Lee^1,2, Shui-Chin Lu³, Yu-Lin Hsieh^1,2

¹Department of Anatomy, School of Medicine, College of Medicine, Kaohsiung Medical University, ²Department of Medical Research, Kaohsiung Medical University Hospital, ³Department of Medical Research, Ultrastructural Laboratory, Kaohsiung Medical University Hospital

Online Transcranial Magnetic Stimulation Protocol for Measuring Cortical Physiology Associated with Response Inhibition

Michael D. Guthrie¹, Donald L. Gilbert², David A. Huddleston², Ernest V. Pedapati^2,3, Paul S. Horn², Stewart H. Mostofsky⁴, Steve W. Wu²

¹College of Medicine, University of Cincinnati, ²Division of Neurology, Cincinnati Children's Hospital Medical Center, ³Division of Psychiatry, Cincinnati Children's Hospital Medical Center, ⁴Center for Neurodevelopmental and Imaging Research, Kennedy Krieger Institute

Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans

Bhavya Ravi*¹, Layla M. Nassar*^1,2, Richard J. Kopchock III*², Pravat Dhakal*², Michael Scheetz², Kevin M. Collins^1,2

¹Neuroscience Program, University of Miami School of Medicine, ²Department of Biology, University of Miami

In Vivo Electrophysiological Measurement of the Rat Ulnar Nerve with Axonal Excitability Testing

Brandon M. Wild¹, Renée Morris¹, Mihai Moldovan², Christian Krarup², Arun V. Krishnan³, Ria Arnold¹

¹School of Medical Science, University of New South Wales, ²Department of Clinical Neurophysiology, Rigshospitalet and the Institute of Neuroscience and Pharmacology, University of Copenhagen, ³Prince of Wales Clinical School, University of New South Wales

Simple Generation of a High Yield Culture of Induced Neurons from Human Adult Skin Fibroblasts

Shelby Shrigley¹, Karolina Pircs¹, Roger A. Barker^1,2, Malin Parmar¹, Janelle Drouin-Ouellet¹

¹Department of Experimental Medical Science, Wallenberg Neuroscience Center, Division of Neurobiology and Lund Stem Cell Center, Lund University, ²John van Geest Centre for Brain Repair & Department of Neurology, Department of Clinical Neurosciences and Cambridge Stem Cell Institute, University of Cambridge

A Novel In Vitro Live-imaging Assay of Astrocyte-mediated Phagocytosis Using pH Indicator-conjugated Synaptosomes

Youkyeong Gloria Byun¹, Won-Suk Chung¹

¹Department of Biological Sciences, Korea Advanced Institute of Science and Technology

Neuroimaging Field Methods Using Functional Near Infrared Spectroscopy (NIRS) Neuroimaging to Study Global Child Development: Rural Sub-Saharan Africa

Kaja K. Jasińska^1,2, Sosthène Guei³

¹Linguistics and Cognitive Science Department, University of Delaware, ²Haskins Laboratories, ³Centre de Recherche et d'Action pour la Paix

The Mouse Hindbrain As a Model for Studying Embryonic Neurogenesis

Mathew Tata¹, Christiana Ruhrberg¹

¹UCL Institute of Ophthalmology

Measurement & Analysis of the Temporal Discrimination Threshold Applied to Cervical Dystonia

Rebecca B Beck*¹, Eavan M McGovern*^1,2,3, John S Butler⁴, Dorina Birsanu¹, Brendan Quinlivan¹, Ines Beiser^1,2,3, Shruti Narasimham¹, Sean O'Riordan^2,3, Michael Hutchinson^2,3, Richard B Reilly^1,5

¹School of Engineering, Trinity College Dublin, The University of Dublin, ²Department of Neurology, St. Vincent's University Hospital, ³School of Medicine and Medical Sciences, University College Dublin, ⁴School of Mathematical Sciences, Dublin Institute of Technology, ⁵School of Medicine Trinity College Dublin, The University of Dublin

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