"Hanging drops" Protocols and Video Articles

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Shrinky-Dink Hanging Drops: A Simple Way to Form and Culture Embryoid Bodies

Chi-Shuo Chen¹, Jonathan Pegan¹, Jesus Luna¹, Bing Xia¹, Kara McCloskey¹, Wei-chun Chin¹, Michelle Khine¹

¹School of Engineering, University of California Merced - UC Merced

Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions

Joni H. Ylostalo¹, Nikolay Bazhanov², Arezoo Mohammadipoor³, Thomas J. Bartosh⁴

¹Department of Biology, University of Mary Hardin-Baylor, ²Department of Pediatrics, University of Texas Medical Branch, ³Multi-Organ Support Technology Task Area, U.S. Army Institute of Surgical Research, ⁴Internal Medicine, Texas A&M University Health Science Center

Differentiation of Atrial Cardiomyocytes from Pluripotent Stem Cells Using the BMP Antagonist Grem2

Jeffery B. Bylund^1,2, Antonis K. Hatzopoulos^1,3

¹Department of Medicine, Division of Cardiovascular Medicine, Vanderbilt University School of Medicine, ²Department of Pharmacology, Vanderbilt University, ³Department of Cell & Developmental Biology, Vanderbilt University School of Medicine

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells

Peter Szaraz*^1,2, Yarden S. Gratch*¹, Farwah Iqbal^1,2, Clifford L. Librach^1,2,3,4,5

¹Create Fertility Centre, ²Department of Physiology, University of Toronto, ³Department of Obstetrics and Gynecology, University of Toronto, ⁴Department of Physiology, University of Toronto, ⁵Department of Obstetrics and Gynecology, Women's College Hospital

Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies

Junning Yang¹, Chuanshen Wu², Ioana Stefanescu¹, Arie Horowitz^1,3

¹Department of Medicine, Cardeza Vascular Research Center, Sidney Kimmel Medical College, Thomas Jefferson University, ²Department of Molecular Cardiology, Cleveland Clinic Foundation, ³Department of Cancer Biology, Cardeza Vascular Research Center, Sidney Kimmel Medical College, Thomas Jefferson University

A Cancer Cell Spheroid Assay to Assess Invasion in a 3D Setting

Eric B. Berens¹, Jon M. Holy², Anna T. Riegel¹, Anton Wellstein¹

¹Lombardi Comprehensive Cancer Center, Department of Oncology, Georgetown University, ²Department of Biomedical Sciences, University of Minnesota

Characterizing Cell Migration Within Three-dimensional In Vitro Wound Environments

Seema Nandi^1,2, Ashley C. Brown^1,2

¹Joint Department of Biomedical Engineering, North Carolina State University and The University of North Carolina - Chapel Hill, ²Comparative Medicine Institute, North Carolina State University

Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram

Jonathan A. Coleman¹, Evan M. Green², Eric Gouaux^1,3

¹Vollum Institute, Oregon Health & Science University, ²Graduate Group in Biophysics, University of California, San Francisco, ³Howard Hughes Medical Institute, Oregon Health & Science University

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation

Emmanuel Bikorimana^1,2, Danica Lapid³, Hyewon Choi³, Richard Dahl^1,2,3

¹Harper Cancer Research Institute, ²Microbiology and Immunology, Indiana University School of Medicine, ³Department of Biological Sciences, University of Notre Dame

Robotic Production of Cancer Cell Spheroids with an Aqueous Two-phase System for Drug Testing

Stephanie Lemmo Ham¹, Ehsan Atefi¹, Darcy Fyffe¹, Hossein Tavana¹

¹Department of Biomedical Engineering, The University of Akron

The Multi-organ Chip - A Microfluidic Platform for Long-term Multi-tissue Coculture

Eva-Maria Materne*¹, Ilka Maschmeyer*^1,2, Alexandra K. Lorenz¹, Reyk Horland^1,2, Katharina M. S. Schimek¹, Mathias Busek³, Frank Sonntag³, Roland Lauster¹, Uwe Marx^1,2

¹Medical Biotechnology, Technische Universität Berlin, ²TissUse GmbH, ³Fraunhofer IWS

A Simple Hanging Drop Cell Culture Protocol for Generation of 3D Spheroids

¹Department of Surgery, UMDNJ-Robert Wood Johnson Medical School

A Paired Bead and Magnet Array for Molding Microwells with Variable Concave Geometries

Gi-Hun Lee¹, Youngjoon Suh¹, Joong Yull Park¹

¹School of Mechanical Engineering, College of Engineering, Chung-Ang University

Generation of Aggregates of Mouse Embryonic Stem Cells that Show Symmetry Breaking, Polarization and Emergent Collective Behaviour In Vitro

Peter Baillie-Johnson¹, Susanne Carina van den Brink^1,2, Tina Balayo¹, David Andrew Turner¹, Alfonso Martinez Arias¹

¹Department of Genetics, University of Cambridge, ²Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences

Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect

Stephanie Hutin*¹, Gianluca Santoni*¹, Ulrich Zander², Nicolas Foos¹, Sylvain Aumonier¹, Guillaume Gotthard¹, Antoine Royant^1,3, Christoph Mueller-Dieckmann¹, Gordon Leonard¹

¹European Synchrotron Radiation Facility, Structural Biology Group, ²European Molecular Biology Laboratory, ³Univ. Grenoble Alpes, CNRS, CEA, IBS (Institut de Biologie Structurale)

Video Coming Soon

In vitro Differentiation of Mouse Embryonic Stem (mES) Cells Using the Hanging Drop Method

Xiang Wang¹, Phillip Yang¹

¹Division of Cardiovascular Medicine, Stanford University

Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro

Katarzyna Falenta*¹, Sangeetha Gajendra*², Martina Sonego¹, Patrick Doherty¹, Giovanna Lalli¹

¹Wolfson Centre for Age-Related Diseases, King's College London, ²MRC Centre for Developmental Neurobiology, King's College London

Modified Mouse Embryonic Stem Cell based Assay for Quantifying Cardiogenic Induction Efficiency

Ada Ao¹, Charles H. Williams¹, Jijun Hao¹, Charles C. Hong^1,2,3,4

¹Division of Cardiovascular Medicine, Department of Medicine, Vanderbilt University School of Medicine, ²Department of Pharmacology, Vanderbilt University School of Medicine, ³Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, ⁴Research Medicine, Veterans Administration TVHS

Generation of Murine Cardiac Pacemaker Cell Aggregates Based on ES-Cell-Programming in Combination with Myh6-Promoter-Selection

Christian Rimmbach¹, Julia J. Jung¹, Robert David¹

¹Reference and Translation Center for Cardiac Stem Cell Therapy, University of Rostock

Isolating Mesangiogenic Progenitor Cells (MPCs) from Human Bone Marrow

Marina Montali*¹, Serena Barachini*¹, Simone Pacini¹, Francesca M. Panvini¹, Mario Petrini¹

¹Department of Clinical and Experimental Medicine, University of Pisa

Generation of High-Throughput Three-Dimensional Tumor Spheroids for Drug Screening

Lesley Mathews Griner¹, Kalyani Gampa¹, Toan Do², Huyen Nguyen², David Farley², Christopher J. Hogan², Douglas S. Auld², Serena J. Silver¹

¹Oncology Drug Discovery: Molecular Pharmacology, Novartis Institutes for Biomedical Research, ²Chemical Biology and Therapeutics, Novartis Institutes for Biomedical Research

Three-Dimensional Culture Assay to Explore Cancer Cell Invasiveness and Satellite Tumor Formation

Marie-France Côté¹, Audrey Turcotte^1,2, Charles Doillon^1,3, Stephane Gobeil^1,2

¹CHU de Québec Research Centre, ²Department of Molecular Medicine, Laval University, ³Department of Surgery, Laval University

Assaying the Ability of Diffusible Signaling Molecules to Reorient Embryonic Spinal Commissural Axons

Virginia M. Hazen*^1,2, Keith Phan*¹, Ken Yamauchi*^1,2, Samantha J. Butler^1,2

¹Department of Biological Sciences, University of Southern California, ²Neuroscience Graduate Program, University of Southern California

Aggregate Size Optimization in Microwells for Suspension-based Cardiac Differentiation of Human Pluripotent Stem Cells

Celine L. Bauwens*¹, Derek Toms*², Mark Ungrin²

¹Institute of Biomaterials and Biomedical Engineering, University of Toronto, ²Department of Comparative Biology and Experimental Medicine, University of Calgary

Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells

Deborah Merrick¹, Hung-Chih Chen¹, Dean Larner¹, Janet Smith¹

¹School of Biosciences, University of Birmingham

Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software

Koen J. C. Verbrugghe¹, Raymond C. Chan¹

¹Human Genetics, University of Michigan

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