"Issue 17" Protocols and Video Articles

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AC Electrokinetic Phenomena Generated by Microelectrode Structures

Robert Hart¹, Jonghyun Oh², Jorge Capurro², Hongseok (Moses) Noh²

¹Biomedical Engineering, Science and Health Systems, Drexel University, ²Mechanical Engineering and Mechanics, Drexel University

Culture of myeloid dendritic cells from bone marrow precursors

Jeanette Boudreau^1,2, Sandeep Koshy^2,3, Derek Cummings², Yonghong Wan²

¹Medical Sciences Program, McMaster University, ²Centre for Gene Therapeutics, McMaster University, ³Department of Chemical Engineering, University of Waterloo

In vitro Differentiation of Mouse Embryonic Stem (mES) Cells Using the Hanging Drop Method

Xiang Wang¹, Phillip Yang¹

¹Division of Cardiovascular Medicine, Stanford University

Neutrophil Isolation Protocol

Hana Oh¹, Brian Siano¹, Scott Diamond¹

¹Institute for Medicine and Engineering, University of Pennsylvania

Chromatin Immunoprecipitation from Human Embryonic Stem Cells

Stephane Bertani¹, Alice Kan¹, Frank Sauer¹

¹Department of Biochemistry, University of California - Riverside

Application of a C. elegans Dopamine Neuron Degeneration Assay for the Validation of Potential Parkinson's Disease Genes

Laura A. Berkowitz¹, Shusei Hamamichi¹, Adam L. Knight¹, Adam J. Harrington¹, Guy A. Caldwell¹, Kim A. Caldwell¹

¹Department of Biological Sciences, University of Alabama

Screening for Amyloid Aggregation by Semi-Denaturing Detergent-Agarose Gel Electrophoresis

Randal Halfmann^1,2,3, Susan Lindquist^1,2,3

¹Whitehead Institute for Biomedical Research, ²Department of Biology, MIT - Massachusetts Institute of Technology, ³Howard Hughes Medical Institute

Whole-cell Recordings of Light Evoked Excitatory Synaptic Currents in the Retinal Slice

Birgit Werner¹, Paul B. Cook^1,2, Christopher L. Passaglia^1,3

¹Program in Neuroscience, Boston University, ²Department of Biology, Boston University, ³Department of Biomedical Engineering, Boston University

Major Components of the Light Microscope

Victoria Centonze Frohlich¹

¹Core Optical Imaging Facility, University of Texas Health Science Center at San Antonio (UTHSCSA)

Interview: Protein Folding and Studies of Neurodegenerative Diseases

Susan Lindquist¹

¹Whitehead Institute for Biomedical Research, MIT - Massachusetts Institute of Technology

Single Cell Electroporation in vivo within the Intact Developing Brain

D. Sesath Hewapathirane^1,2, Kurt Haas^1,2

¹Brain Research Centre, University of British Columbia - UBC, ²Department of Cellular and Physiological Sciences, University of British Columbia - UBC

Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging

Melanie P. Matheu¹, Debasish Sen¹, Michael D Cahalan¹, Ian Parker²

¹Department of Physiology and Biophysics, University of California, Irvine (UCI), ²Department of Neurobiology and Behaviour, University of California, Irvine (UCI)

Staining Proteins in Gels

Sean Gallagher¹, Deb Chakavarti²

¹Keck Graduate Institute of Applied Life Sciences, UVP, LLC, ²Proteomic Center, Keck Graduate Institute of Applied Life Sciences

Freezing, Thawing, and Packaging Cells for Transport

Richard Ricardo¹, Katy Phelan¹

¹Molecular Pathology Laboratory Network, Inc

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